Quick Guide xPONENT® for FLEXMAP 3D® Software Version 4.
© Luminex Corporation, 2012. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, or translated into any language or computer language, in any form or by any means without prior express, written consent of Luminex Corporation. LUMINEX CORPORATION 12212 Technology Boulevard Austin, Texas 78727-6115 U.S.A. Voice: (512) 219-8020 Fax: (512) 219-5195 xPONENT® for FLEXMAP 3D® Software Version 4.2 Quick Guide 89-00002-00-402 Rev.
Table of Contents StartingxPONENT® ...............................................................................................................................1 Start-up Indicators .................................................................................................................................1 Common Daily Activities .................................................................................................................1 Software Overview .......................................
xPONENT™ for FLEXMAP 3D® iv
StartingxPONENT® • On the PC desktop, click the Luminex® xPONENT® icon, or click Start > All Programs > Luminex > xPONENT > Luminex xPONENT. • If you have a trial license, contact Luminex® Technical Support to obtain a full license, or click OK in the dialog box to continue. • If this is the first time you have started the software, the User License Agreement may display. Read the license agreement. Select I accept the terms of this license agreement, then click OK.
Software Overview xPONENT® software is organized into a series of pages. As you click each page, applicable tabs open on the left-hand side of the window. xPONENT® opens to the Home page. You can access most tasks from the Home page.
Results - Analyze previously acquired batches; view current and saved reports Protocols - Displays protocols, Stds & Ctrls, and Lot information. Maintenance - Calibrate, verify, and maintain the application Admin - Perform administrative tasks Log Off - Log out of software Help - Access help file System Monitor The System Monitor is displayed at the bottom of all xPONENT® windows. It displays the physical state of the Luminex® system. Values are reported directly from the Luminex® system.
System Status Button - This button has two functions: When clicked, it opens the system log. It also displays the current status of the system. If there are no warnings or errors, the System Status button is green with a check mark. If there is a warning, out of calibration condition, or other important user notification, the button is yellow with an exclamation point. Connection Display - Displays the connection status. Cal/Ver Ok - This will be lit up if the calibrations and verifications are connected.
Adjusting the Sample Probe Height Adjust the sample probe height to ensure that the probe drops far enough into the well to acquire sample. NOTE: Ensure that there is no liquid in the wells or reservoirs before adjusting the sample probe height. NOTE: When you adjust and save the probe height settings for all three areas under a plate name, all areas retain the adjustment. WARNING: Correct sample probe height is critical to successful sample acquisition and calibration.
7. Type a name for the plate in the Plate Name box, or select a saved plate from the Plate Name list. NOTE: If a saved plate is selected the results of the new calibration will override the previous calibration. 8. In the Plate section, click D6 for the 96-well plate. Click H12 for the 384-well plate. 9. In the Reservoir section, click RB1. 10. In the Strip-Wells section, click SD1. 11. Click Auto Adjust Height. The probe automatically adjusts itself and saves it to the plate you selected.
Revive After Storage Routine NOTE: The Revive After Storage routine is necessary when the system has been idle for more than a week. After you have adjusted the sample probe height, run the Revive After Storage (Luminex) routine. 1. Open the Maintenance page, then the Cmds & Routines tab. 2. Select Revive After Storage (Luminex) from the Routine Name drop-down list.
7. Load wells with at least five drops of the Calibration, Verification, and Fluidics beads, as indicated on the plate image and in the kit insert instructions. NOTE: To ensure you get the necessary bead count, invert the calibrator and verifier vials perpendicular to the plate as you add drops to the wells. This ensures the maximum fluid drop size is dispensed into the wells. 8. Click Retract. 9. Click Run.
4. Vortex the xMAP calibrator, verification, and fluidics containers for 30 seconds to ensure homogeneity. Do not dilute xMAP calibrator, verification, or fluidics agents. 5. Click Eject on the status bar. 6. Place the off-plate reagent block on the plate holder. Load a strip well into the block. 7. Load wells with at least five drops of the Calibration, Verification, and Fluidics beads, as indicated on the plate image and in the kit insert instructions.
To run, print, and/or save a report: 1. Open the Results page. 2. Click the Reports tab. 3. In the Report drop-down list, select the report that you want to view. 4. In the Type drop-down list, select the type of report you want to view. NOTE: Select dates if applicable. 5. Select the item for which you want to generate the report. If you are creating a Batch Report, select the analytes to include in the report. 6. Click Generate. 7.
You can create each quantitative, qualitative, and allele call protocols using one product composed of a definable number of standards and controls. There are three steps to creating a protocol. All three are accessible from the Protocols page, Protocols tab. The three steps are: • Define settings. • Select the analysis type. • Define the plate layout. To create a protocol: 1. Open the Protocols page. 2. Click Create New Protocol. The Settings tab opens. 3.
6. Select Fit of all Standards or Mean of Replicates. 7. Select Analyze results while acquiring samples if you want to view real-time analysis. 8. Click Next to open the Select Analytes tab. Select analytes by clicking the bead number. The table allows you to name the analyte and to select the analysis type, the units to be measured, and the count. 9. Click Change to change the analysis settings. 10. Click Next. The Plate Layout tab opens.
11. Add standards, controls, unknowns, and maintenance steps to the plate layout. 12. Click Save to save the protocol. Creating a Kit To create a kit: 1. Open the Protocols page, then open the Protocols tab. 2. Select the protocol that you want to use for the kit, then click New Std/Ctrl. The Std/Ctrl Details tab opens. 3.
Create a Batch There are three batch creation options: • Create a New Batch from an Existing Protocol (uses a protocol; described in this section). • Create a New Batch from a New Protocol • Create a New Multi-batch (runs multiple batches at one time). To create a new batch from an existing protocol: 1. Open the Batches page. 2. Click Create a New Batch from an Existing Protocol. 3. Type the batch name in the Batch Name box. 4. Type a description of the batch in the Enter Optional Description box.
2. Select the Current Batch or Saved Batches tab. 3. If you want to open a saved batch, highlight the batch and click Open. Shut Down the Instrument Run the daily shutdown routine to prevent clogs and crystallization of salt in the sample probe. Clogs and crystallization of salt in the sample probe may cause problems with calibration, verification, and data acquisition; they may also cause sample splashing. Shut down the system properly to ensure system integrity. 1. On the Home page, click Shutdown.
By Phone Inside the U.S. and Canada Contact Luminex Technical support at: • Toll Free: 1-877-785-BEAD (-2323) • Fax: 512-219-5114 By Phone Outside the U.S. and Canada Contact Luminex Technical support at: • International Toll Free: +800 2939 4959 • Direct Phone: +1 512-381-4397 • Fax: +1 512-219-5114 Email Email questions to support@luminexcorp.com.
