® xPONENT 3.1 Rev.
© Luminex Corporation, 2011. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, or translated into any language or computer language, in any form or by any means without prior express, written consent of Luminex Corporation. LUMINEX CORPORATION 12212 Technology Boulevard Austin, Texas 78727-6115 U.S.A. Voice: (512) 219-8020 Fax: (512) 219-5195 xPONENT® 3.1 Rev. 2 Software Manual PN 89-00002-00-235 Rev.
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PROFITS, FAILURE TO REALIZE SAVINGS, LOSS OF PRODUCTS OF BUYER OR OTHER USE OR ANY LIABILITY OF BUYER TO A THIRD PARTY ON ACCOUNT OF SUCH LOSS, OR FOR ANY LABOR OR ANY OTHER EXPENSE, DAMAGE OR LOSS OCCASIONED BY SUCH PRODUCT INCLUDING PERSONAL INJURY OR PROPERTY DAMAGE UNLESS SUCH PERSONAL INJURY OR PROPERTY DAMAGE IS CAUSED BY SELLER'S GROSS NEGLIGENCE. 3.
and Buyer receives no rights to such intellectual property rights by virtue of its purchase of Product other than as expressly set forth herein. Buyer shall have no right to use any trademarks owned or licensed to Luminex without the express written permission of Luminex. 4.
End-User License Agreement (EULA) for Luminex® xPONENT® Software This Luminex End-User License Agreement (“EULA”) is a legal agreement between you (either an individual or a single entity, also referred herein as “you”) the end-user and Luminex Corporation (“Luminex”) regarding the use of the xPONENT software product provided to you above, which includes computer SOFTWARE and online or electronic documentation and may include associated media and printed materials (if any) (“SOFTWARE”).
3. GRANT OF LICENSE. Subject to the terms and conditions of this EULA, Luminex hereby grants to you a nonexclusive, nontransferable, nonassignable license (without right to sublicense) under Luminex's copyrights and trade secrets to use the SOFTWARE on a single computer running with a single unit of a specific model of Luminex instrument, as such model is identified on the packaging included with the SOFTWARE. You may make one (1) copy of the SOFTWARE for backup or archival purposes only.
5. TERM AND TERMINATION. Your rights under this EULA are effective until termination. You may terminate this EULA at any time by destroying the SOFTWARE, including all computer programs and documentation, and erasing any copies residing on your computer equipment. Luminex may terminate this EULA upon thirty (30) days written notice to you. Your rights under this EULA automatically terminate without further action on the part of Luminex if you do not comply with any of the terms or conditions of this EULA.
xPONENT® 3.1 Rev.
Table of Contents Chapter 1 Introduction ........................................................................................................1 Software Packages ......................................................................................................................................1 About this Guide ..........................................................................................................................................1 Warnings, Notes and Symbols .........................
Auto Maint Tab ....................................................................................................................................60 Lot Management Tab ..........................................................................................................................61 Cmds & Routines Tab .........................................................................................................................62 Probe and Heater Tab .....................................................
Delete Batch ......................................................................................................................................104 Edit Batch ..........................................................................................................................................104 Running a Pending Batch ..................................................................................................................104 Performing Analysis ..............................................
xPONENT® 3.1 Rev.
Chapter 1: Introduction The Luminex system was developed to improve workflow and efficiency in the clinical laboratory and in research and development. End users in the laboratory must run microtiter plates using xMAP®-based assay kits developed for use with xMAP technology. Software Packages xPONENT® software has different levels of functionality: • Allows you to perform analysis, create protocols and batches, review and print reports, and perform Luminex system maintenance.
The conventions in this document assume a basic familiarity with computers and a knowledge of Microsoft® Windows® software. Commands are often available through more than one method, such as from the toolbar and from menus that appear when right-clicking an area of the screen. However, for ease of use the individual procedures in this manual describe only one method for accessing commands. This guide is formatted as a PDF, and can be printed.
Use of unauthorized third-party software with xPONENT software may result in corruption or failure of the xPONENT software. Use third-party software at your own risk. If using a screen saver on the xPONENT system, be aware that xPONENT will prevent it from activating. A dialog box will display each time xPONENT is launched, recommending that the screen saver and any power management settings are turned off.
medical advice immediately and show the product label or container to your medical provider. A Material Safety Data Sheet is available upon request. WARNING: Reagents may contain sodium azide as a preservative. Sodium azide may react with lead and copper plumbing to form highly explosive metal azides. It is also highly toxic and rates a material safety data sheet (MSDS) health hazard 4. On disposal, flush drains with a generous amount of cold water to prevent azide build-up.
TABLE 2. Reporter Fluorchromes Wavelengths R-Phycoerythrin Alexa 532 Formula weight (Daltons) 240,000 470 Absorbance max (nm) 480, 546, 565 531 Extinction max (M-1cm-1) 1,960,000 83,800 Emission max (nm) 578 554 Quantum yield 0.82 0.8 Sample Volume Sample volumes or sample sizes range from 10 to 200 µL. Ensure that some sample remains in the well after aspiration; about 25 µL greater than the sample volume. This amount may vary depending on the type of plate used.
Gating information may change with a new lot of xMAP beads. Each time you receive a new lot of xMAP beads, evaluate them with the current protocols. If gating information changes, create a new protocol identical to the current protocol, but with a new version number and new gating information. If running an xMAP-based kit, follow the instructions found on the kit’s product insert or use the provided software protocol.
Chapter 2: Touring the Software This chapter explores each section of the software and its functionality. For information about using the software, see Using the Software. This chapter describes default views. Please note that views can be changed and functionality can be disabled by the xPONENT® system administrator. Log In To log in, type your user ID at the System Login tab. If you are using the secure version of the software, type your password. Once you have logged in, the Home page opens.
FIGURE 1. 1 Help Icons System-wide Help 2 Help for Current Window Log Off Click Log Off to log out of the software, then click OK in the Confirm Logout dialog box. Exit After you log out, click Exit, then Yes to exit the software completely. Screen Elements This section details the screen elements and the common terms used in this manual to describe them. xPONENT® 3.1 Rev.
FIGURE 2. 1 Page 2 Tab Screen Elements 3 Right-click Menu Page The main elements at the top of the window are Pages. Click a page to go to that page in the software. All of the pages except the Home page, and in some cases the Admin page, can be moved or deleted. For additional information, see the System Setup Tab section. Tab The elements on the left side of the window are Tabs. Click a tab to display information and tasks on that tab.
• Export - Opens a File Dialog dialog box. Click Browse to select a location, file name, and file type (.txt or .csv) for the export. This exports all data from the right-clicked item • Cut - Cuts the selected data • Copy All - Copies all data • Copy - Copies only selected data • Paste - Pastes previously copied text or data into the box • Delete - Erases text or data from the selection The histogram and dot plot within the Results page have specialty right-click menus.
• Pause - Pauses the system after the current command completes. Pause does not stop the system in the middle of running a command. You cannot run another command while the system is paused. • Stop - Stops the system, regardless of command status. • Eject - Ejects the plate. Once the plate is ejected, the Eject button changes to Retract. Retract retracts the plate, and the Retract button changes back to Eject.
FIGURE 4. Home Page Return to the Home page at any time by clicking Home in the Navigation toolbar. This page contains the following: • Click to Create a new Batch using the highlighted Protocol below - Creates a new batch using a selected protocol from the Installed Protocols list. For more information about creating a batch from an existing protocol, see Create a New Batch from an Existing Protocol. • Installed Protocols - Displays a list of protocols.
• View - Opens the Settings tab of the Protocols page to view the selected protocol. This tab enables viewing the settings, analytes, and plate layout for the selected protocol. • Daily Activities - Contains shortcut buttons to common commands in the xPONENT software: • System Initialization - Opens the System Initialization command in the Auto Maint tab on the Maintenance page. For more information about this tab, see Auto Maint Tab.
Sample Lists - Contains a list of protocols, including the version number and the number of samples associated with each protocol. Details - Opens the Edit Samples tab to view or edit sample details for the selected protocol. Create Batch - Opens the Protocol tab to create a batch. Create Sample Tab Use this tab to type and view sample information. FIGURE 5. Create Sample Tab This tab contains the following: • Protocol - Displays the protocol selected in the Samples tab.
The following Delete, New, Edit, and Undo buttons only display depending on actions taken in the Create Sample tab. • Delete - Deletes a highlighted sample. • New - Creates a new sample. • Edit - Edits a highlighted sample. • Undo - Reopens the Create Sample tab without saving any changes made using the Edit or New buttons. • Save - Saves changes made to the Sample list. • Close - Returns to the Samples tab.
FIGURE 6. Batch Plate Layout Report • Import - Imports a batch. • Export - Exports a batch. • Delete - Deletes a batch. • Edit - Edits a batch. • Run - Runs a batch. Create a New Batch from an existing Protocol Creates a New Batch from an existing protocol. The Protocol tab opens. Protocol Tab Use this tab to name a batch, type a description, select a protocol, and view active reagents. xPONENT® 3.1 Rev.
FIGURE 7. Protocol Tab This tab contains the following: Batch Name/Description - Contains the protocol name, version, manufacturer, and creation date for each protocol. Select a Protocol - Contains the protocol name, version, manufacturer, and creation date for each protocol. Active Reagents - Displays assay and control lots/kits associated with the selected protocol.
FIGURE 8. Stds & Ctrls Tab This tab contains the following: Apply Std/Ctrl Kit - Opens the Select Std/Ctrl Kit dialog box. The dialog box displays the Std/Ctrl Kit Lot #, Std/Ctrl Kit Name, Expiration, and Manufacturer for the kit. Select a Std/Ctrl kit from the list and then clickOK to close the dialog box. The kit information will display in the boxes to the right of the Apply Std/Ctrl Kit button. The selected kit must be associated with the same analyte names.
• Dilution - Contains the following dilution options: • 1:2 - Halves the standard from each previous iteration. • 1:10 (Log) - Computes a value of one-tenth of the standard from each previous iteration. • 1/2 Log - Creates a 1:3.16 dilution, or half of each 1:10 (Log) from each previous iteration. • Alternatively, type a number for your own dilution factor. • Apply Dilution - Applies the dilution selected in the Dilution list. NOTE: Click a column header to resort the display.
FIGURE 9. Plate Layout Tab Create a New Batch From a New Protocol Click Create a New Batch from a new Protocol to create a new batch without using a protocol. The Settings tab opens. Raw data results write to a.CSV file. You can define parameters for samples, gates, regions, events, and commands. NOTE: If the batch spans more than one plate, the tray ejects automatically when all defined wells have been acquired. A dialog box displays prompting you to insert the next plate.
FIGURE 10.
• Name and Description boxes - Type a name and a description in the appropriate boxes. • Acquisition Settings - Use this section to set the following: • Bead Type - Select either MicroPlex (standard) or MagPlex (magnetic) beads. • Volume - This is the volume the instrument will aspirate into the system for analysis. Type the desired sample volume in microliters. Use values from 10 to 200 µl for for 96well plates. To avoid air intake, add at least 25 µl to the sample well in addition to the sample size.
• Analysis Settings - Use this section to set the analysis type, set the number of standards and controls, select an external analysis program, and choose whether to analyze results while acquiring samples. Analysis Type - Use this list to choose from the following analysis types: • None - No analysis. Select if you have your own data post-processing program and want to obtain only fluorescent intensity results. You cannot apply standards or controls when you select None.
• Min MFI Enabled - Select this box to enable a minimum MFI for the Allele Call analysis. Type a value in this box to set the minimum MFI for analysis. • Number of Standards - Click to type the number of standards for the protocol. • Number of Controls - Click to type the number of controls for the protocol. • Fit of all Standards - The standard curve will be determined by using each individual standard replicate when calculating the standard curve.
FIGURE 11. Analysis Settings Dialog Box If you selected Quantitative on the Settings tab, the default analysis formula is 5P Weighted. To change the default, select one of the following from the Method list: • No Analysis • Cubic Spline • Linear Fit • Logistic 4P • Logistic 5P If you selected Logistic 4P or Logistic 5P, select a weight type of either None or 1/y2. If you selected Qualitative on the Settings tab, the default analysis is Luminex Qualitative.
not enough bead events were acquired. If you select bead sets that are not present, the analyzer continues to acquire, trying to reach the number of events per bead for bead sets that are not in the sample. Therefore, choose only the bead sets present in your sample. Apply All - Applies the information in the Units and Counts boxes to all analytes. Total Count - Select Stop after bead count reaches: to stop acquisition when the bead count reaches a certain number determined by the user.
• Select a method from the Method list. • If necessary, select a weight type from the Weight Type list. • Apply the analysis to all analytes in the list by clicking Apply to All Analytes. • Select Mark as Intra-Well Normalization Bead to make the analyte an intra-well normalization bead. • Add a range to the analysis by clicking Add Range. • Select Use Threshold Ranges to enable ranges for the analysis. • Click Add Range to add a range.
Plate Layout Tab Use this tab to select wells to add samples and maintenance commands to the plate. For more information about the functionality of this tab, see “Plate Layout Tab” section. FIGURE 14. Plate Layout Tab Create a New Multi-Batch Batches > Batches > New Multibatch Use this button to add or remove pending batches to the multi-batch set up and to run a multi-batch. A multi-batch is composed of one or more pending batches which, together, will fit on one plate.
This page contains the following: Multi-Batch Name - Use this field to create a name for new multi-batches. Select Pending Batch - Contains a list of all pending batches. The list includes name, protocol, protocol version, date, and status information for each pending batch. Select the batch you want to add to the plate. Click OK. A plate layout diagram automatically populates the wells for the batch. Click Add to open this box again and add additional batches. FIGURE 15.
Plate Layout - Depicts where batches are located on the plate. Multi-Batch - Lists pending batches selected for the multi-batch. The list includes name and “Start at” well. Plate Layout - Opens the Multibatch Report dialog box that contains: • Page - Use these arrows to scroll through the report pages. • Zoom - Select from the list to change the report magnification. • Print - Prints report. • Save - Saves report. • Close - Closes report dialog box.
• LIS Results • Reports Current Batch Tab Use this tab to view results, statistics, and log information related to the current batch, and to perform statistical analysis on batch results. This tab offers real-time monitoring of batch sampling during acquisition through a display of sample bead statistics and analytes, histogram, and dot plot data. The statistics available on this tab are intrawell bead statistics. They do not describe replicate well assay results.
• % CV of microspheres - The measure of relative dispersion within the distribution. %CV = 100 x Std Dev / Mean • Standard Deviation - For calculating sample variability or dispersion, Luminex uses the standard deviation formula. • Peak - The value that is equal to the largest number of data points within the distribution. For example, in data set {1,2,2,3,3,3,4,5}, 3 is the peak because it occurs the largest number of times in the distribution list.
Both the histogram and the dot plot have buttons that enable you to change the default view: Click the maximize button to maximize the graph to fill the whole window. In the maximized view, use the sliders to zoom in or zoom out. To return to the standard view, click the minimize button. If the histogram or dot plot display have been changed to another view (e.g. Progress, Well Report), click Default to return to the default histogram and dot plot displays.
Right-click inside the dot plot area to access the following options: X Axis - Changes the X axis to the following: Doublet Discriminator, Reporter 1, Classification 1, and Classification 2 NOTE: You can change the X axis of the dot plot for troubleshooting purposes. Use only the default settings in all other instances. • Y Axis - Changes the Y axis to the following: Doublet Discriminator, Reporter 1, Classification 1, and Classification 2 • View - Choose to view all events or only gated events.
Default - Returns to the histogram and dot plot display. Saved Batches Tab Use this tab to open a batch that has been run and view its details, and export, approve, or replay a batch. FIGURE 17. Saved Batches Tab Completed Batches - Displays a list of completed batches that includes the Name, Protocol, Protocol Version, Date, Status, and User information, for each batch. Batches that have not been run are not included. Filter - Opens the Filter Setup dialog box.
FIGURE 18. Filter Setup Dialog Box This dialog box lets you choose the saved batch that you want to display in the Completed Batches list, based on the options you select or clear in the following check boxes: • Batch Name • Protocol • Batch Status • Lot ID • Kit ID • Analyte • Sample ID • First Name • Last Name • User ID • Date Reset - Clears all check boxes. OK - Closes the dialog box and applies any changes you made. Cancel - Closes the dialog box and cancels any changes you made.
FIGURE 19. Batch Plate Layout Report Approve - Opens the Batch Approval Confirmation dialog box to approve the selected batch. Only approved batches can be transmitted to the LIS. If your software is licensed for LIS use, you can transmit batches to the LIS from the Sample Results tab. Once you have approved a batch, the status of the batch changes to Approved in the Completed Batches list. Exp Results - Opens the Save As dialog box to choose an export destination for the .
Replay Batch - Uses the data stored in the run files from the initial acquisition to reprocess a batch, creating a new batch output file. A batch can be reprocessed multiple times. NOTE: The initial batch data and output file always remain intact and unchanged. Each time you replay a batch, the system handles it as if it is a new batch, creating a separate batch entry and output file. Replay a batch to replay the raw bead data files.
Results Tab FIGURE 20. Results Tab The Results tab has the same Save Image, Progress, and Well Report buttons as the Current Batch tab. For more information about these buttons, see Current Batch Tab. The following buttons are added to the Results tab: • Formula - Opens the Change Analysis dialog box with a list of analytes used in the batch. Click an analyte to open the Analysis Settings dialog box from which you can select a new analysis setting for the analyte.
• Analyze - Analyzes data that has been invalidated. This button only appears if you have clicked Invalidate on a portion of the data, or if you have clicked Validate on an invalidated portion of the data. This will analyze the data again with the invalidated standard removed. • Close - Closes the batch and reopens the Saved Batches tab. Settings Tab Use this tab to view the acquisition parameters of the selected saved batch and print the batch settings report. FIGURE 21.
FIGURE 22. Log Tab This tab displays the following information about each activity: • Date • Message • Code Log entries display yellow if a well was acquired but there was a possible problem. They display red if a problem occurred during the acquisition. Print - Prints the log. Export - Opens the Save As dialog box to save the batch log file. Select a location and click Save. Close - Reopens the Saved Batches tab. Sample Details Tab Use this tab to view sample results.
FIGURE 23. Sample Details Tab Arrows - Scrolls through the sample details. Transmit - For systems configured for LIS transmission, select a single analyte or the entire sample and click Transmit to send the results. Close - Reopens the Saved Batches tab. LIS Results Tab This tab displays saved batches containing LIS samples. xPONENT® 3.1 Rev.
FIGURE 24. LIS Results Tab Filter - Opens the Filter Setup dialog box. Clear - Click to turn off the filter. Completed Samples - Displays Name, Protocol, Sample Count, Date, Status, and User information for each batch displayed in this list. Transmit - Transmits a batch to an LIS if xPONENT is connected to one. Details - Opens the Sample Details tab to view sample results. Reports Tab Use this tab to view, generate, and print reports.
FIGURE 25. Reports Tab Report and Type lists. Choose a report from one of the following categories: Batch Reports - A list of batches appears below the Type list. The list includes Name, Protocol, Protocol Version, Date, Status, and User information. Choose from the following reports: • Data Interpretation • Batch Settings • Plate Layout • Batch Audit • Patient Report If you choose Data Interpretation, a Select Analytes section appears on the right and displays a list of the analytes in the batch.
Print All - Click to print the information for all the analytes in the report. Save - Click to open the Save As dialog box. Select a location and click Save. This saves only the analyte information currently being viewed. Print - Click to print the analyte information currently being viewed. New Report - Click to return to the main Reports window.
Protocols The Protocols page enables you to add standards and controls. This page contains the following tabs: • Protocols • Settings • Analytes • Plate Layout • Stds & Ctrls Protocols Tab Use this tab to import, export, or delete a protocol. You can also add new assay standard and or control information using one of the installed protocols. FIGURE 26. Protocols Tab This tab contains the following: Create New Protocol - Opens the Settings tab to create a new protocol.
New Std/Ctrl - Opens the Std/Ctrl Details tab. For more information, see the “Std/Ctrl Details Tab” section. Plate Layout - Opens the Report dialog box, which includes the Plate Layout Report. For an example, see the Protocols Plate Layout Report image below. FIGURE 27. Protocols Plate Layout Report Delete - Opens the Delete Protocol dialog box to delete a protocol selected in the Installed Protocol list. Click Yes to delete the selected protocol. Import - Opens the Open dialog box to select a protocol (.
FIGURE 28. Settings Tab This tab contains the following: xPONENT® 3.1 Rev.
• Name and Description boxes - Type a name and a description in the appropriate boxes. • Acquisition Settings - Use this section to set the following: • Bead Type - Select either MicroPlex (standard) or MagPlex (magnetic) beads. • Volume - This is the volume the instrument will aspirate into the system for analysis. Type the desired sample volume in microliters. Use values from 10 to 200 µl for for 96well plates. To avoid air intake, add at least 25 µl to the sample well in addition to the sample size.
• Analysis Settings - Use this section to set the analysis type, set the number of standards and controls, select an external analysis program, and choose whether to analyze results while acquiring samples. Analysis Type - Use this list to choose from the following analysis types: • None - No analysis. Select if you have your own data post-processing program and want to obtain only fluorescent intensity results. You cannot apply standards or controls when you select None.
• Min MFI Enabled - Select this box to enable a minimum MFI for the Allele Call analysis. Type a value in this box to set the minimum MFI for analysis. • Number of Standards - Click to type the number of standards for the protocol. • Number of Controls - Click to type the number of controls for the protocol. • Fit of all Standards - The standard curve will be determined by using each individual standard replicate when calculating the standard curve.
FIGURE 29. Analysis Settings Dialog Box If you selected Quantitative on the Settings tab, the default analysis formula is 5P Weighted. To change the default, select one of the following from the Method list: • No Analysis • Cubic Spline • Linear Fit • Logistic 4P • Logistic 5P If you selected Logistic 4P or Logistic 5P, select a weight type of either None or 1/y2. If you selected Qualitative on the Settings tab, the default analysis is Luminex Qualitative.
not enough bead events were acquired. If you select bead sets that are not present, the analyzer continues to acquire, trying to reach the number of events per bead for bead sets that are not in the sample. Therefore, choose only the bead sets present in your sample. Apply All - Applies the information in the Units and Counts boxes to all analytes. Total Count - Select Stop after bead count reaches: to stop acquisition when the bead count reaches a certain number determined by the user.
• Select a method from the Method list. • If necessary, select a weight type from the Weight Type list. • Apply the analysis to all analytes in the list by clicking Apply to All Analytes. • Select Mark as Intra-Well Normalization Bead to make the analyte an intra-well normalization bead. • Add a range to the analysis by clicking Add Range. • Select Use Threshold Ranges to enable ranges for the analysis. • Click Add Range to add a range.
Grouping - Selects the sequence in which the replicates are laid out in plate wells. The options are: • 123123123. . . Lays out one of each replicate set at a time in numerical order. • 111222333. . . Lays out all the replicates in a set before moving on to the next set in numerical order. You can assign the following well commands. Each command is associated with a color.
Off Plate Area - Designates an alternate location for maintenance commands in the Commands: list. Highlight a command, and then click the reservoir in the Off Plate Area as the location for the command. Cancel - Returns to the main Protocols tab without saving. Back - Returns to the Analytes tab. Save - Saves the protocol and returns to the main Protocols tab. Stds & Ctrls Tab Use this tab to delete, edit, export, import, and create standards and controls. FIGURE 31.
• Reagent • Lot # • Expiration • Name • Manufacturer • Protocol • Version • Std/Ctrl Kit# • Std/Ctrl Kit Name Select Std/Ctrl Kits Only in the Show selections to display the following information about the installed Std/Ctrl Kits: • Std/Ctrl Kit # • Std/Ctrl Kit Name • Expiration • Manufacturer • Created with Protocol • Version Delete - Deletes a selected lot. The Delete Lot(s) Confirmation dialog box opens. Click Yes to delete the lot. Edit - Opens the Std/Ctrl Details tab to edit the lot information.
FIGURE 32. Std/Ctrl Details Tab This tab contains the following: Apply Std/Ctrl Kit - Opens the Select Std/Ctrl Kit dialog box. The dialog box displays the Std/Ctrl Kit Lot #, Std/Ctrl Kit Name, Expiration, and Manufacturer for the kit. Select a Std/Ctrl kit from the list and then click OK to close the dialog box. The kit information will display in the boxes to the right of the Apply Std/Ctrl Kit button. The selected kit must be associated with the same analyte names.
Assay Standard Information - Displays the selected standard reagents in a list. The list displays the Reagent, Name, Lot #, Expiration, Manufacturer, and expected concentration value of each analyte. • Apply Std Lot - Opens the Select Lot dialog box. Select a lot from the list and then click OK to apply the lot. • Apply Values - Applies a value across or down the Reagent,Name, Lot #, Expiration, and Analyte fields.
• System Status • Schedule • Support Utility Auto Maint Tab This tab contains routines to initialize, verify, and maintain the analyzer. FIGURE 33. Auto Maint Tab Automated Maintenance Options - Provides buttons for commonly used maintenance routines for the machine. Select from the following: • System Initialization - Select this routine to initialize the system. • Performance Verification - Select this routine to use with the Performance Verification kit.
Reagents - Displays the reagents and well locations necessary for the selected routine. You can select kits for the routines from the Calibration Kit and Performance Verification Kit lists. In the lower right section of the Reagents area is a depiction of the Automated Maintenance Plate. Place reagents in the wells of the Automated Maintenance Plate the way they are placed in the depiction. Report - Opens the Reports tab, with the Performance Verification Reports selection displayed in the Report: list.
Active Reagents - Use this section to change calibration or performance verification kits, lot numbers, and expiration dates, or to delete a kit. • Calibration Kit - Select a calibration kit from this list. Details of the selected kit display in the Lot Type, Active Lot Number, and Expiration Date fields. • Performance Verification Kit - Select a performance verification kit from this list. Details of the selected kit display in the Lot Type, Active Lot Number, and Expiration Date fields.
FIGURE 35. Maintenance Commands and Routines Tab This tab contains the following: Routine Name - This list of pre-set routines and commands can be used for system maintenance. Some of these commands are also available on the Auto Maint tab. Additionally, you can create routines on this tab, which display in the Routine Name list once you save the routine. To create a routine, start with None and add the desired commands. You can also modify a Luminex routine and save it as a new routine.
Commands - This section contains the following commands: • Wash - Sends distilled water through the fluidics lines in the system. It pulls the fluid from a well or from the reservoir and runs it completely through the system to the waste receptacle. • Soak - Prevents salt crystals from forming in the probe due to air exposure. Soaking the probe replaces sheath fluid in the probe with water. You should perform the soak function at the end of each day. The system uses at least 250 µL of distilled water.
NOTE: When you modify an existing routine, by adding or clearing a command, the routine name defaults to None in the Routine Name dropdown menu and the Save As button appears. Remember to save your new routine to make it available for future use. Command Sequence - When you select a routine, the individual commands in the routine appear in order in the Command Sequence list. The list includes the name of each command, location, status, and any additional information.
FIGURE 36. Probe and Heater Tab Move Probe Down - Moves the probe down in the specified well(s). Plate Type - Defines the selection of either a 96-well plate or an Automated Maintenance plate. Plate Image - Identifies the location to be used to adjust the probe height. Clicking a well selects it and marks it with a blue pin. Plate Heater - Sets the temperature of the plate to the number typed in the Set Temperature box.
FIGURE 37.
• Last CON2 Verification • Delta Calibration Temp • System Temperature • DD Temperature • CL1 Temperature • CL2 Temperature • CL1 Voltage • CL2 Voltage • DD Voltage • RP1 Voltage Items in this list relating to CAL/Verification, CON calibration, and controls have one of the following states: • Passed - Indicates that the process completed successfully. • Failed - Indicates that the process was not completed successfully. Failed items appear in red. • Not Current - Indicates that verifiers are not current.
FIGURE 38. System Status Tab This tab contains the following: Search By Log Type - Filters the type of log information. Select All, Maintenance, Security, or Warnings and Errors. Search By Log Date - Enables using a date range to display a log of system activities. Advanced - Displays the following added information in the system log: • Log Type • User ID • Error Level Log - Displays a list of information for each system process.
Schedule Tab Use this tab to view reminders for scheduled maintenance due to be performed on the analyzer. FIGURE 39. The Schedule Tab The Reminders section displays Subject, Reminder, Next Alert Date, Alert Time, and Notes. This schedule can be changed using the Schedule tab of the Admin page. Support Utility Tab Use this tab to type information that will be useful in the event you need to contact Luminex Technical Support.
FIGURE 40. Support Utility Tab This tab contains the following: Name, Phone, Company, and Email - Type your information in these boxes so that Luminex Technical Support can contact you. Clear - Clears all information in the Name, Phone, Company, Email, and Comment boxes. Comment - Type any comments in this box. Include Batch info - Includes batch information from a selected batch in the Support Utility.zip export folder. Select Batch - Displays a list of all batches.
• User Setup • Batch Options • Alert Options • CSV Options • Archive • Licensing • Schedule • Report Options System Setup Tab Use this tab to configure system settings. FIGURE 41. System Setup Tab Application Settings These settings change some of the basic ways that xPONENT® works, for graphical user interface preferences. Enable Virtual Keyboard - Enables the touchscreen virtual keyboard.
LIS Settings This setting is only for those using xPONENT® with a Lab Information System (LIS). Connect to LIS - Enables connection the LIS. Browse - Opens the Browse for Folder dialog box. Select a location for the system to watch for incoming orders. External Analysis Program Settings This option is for those using a program other than xPONENT to analyze collected data. Installed Analysis Programs - Lists the analysis programs currently installed.
Disable Automatic launching of External Third Party Analysis when batches complete for all protocols - Disables the automatic launch of the third-party analysis program automatically after batch acquisition. Arrange Main Navigation buttons These options enable you to rearrange the main page headers at the top of the xPONENT® window. Arrange Main Navigation Buttons - Select or clear the check boxes to display or remove main page headers. The Home page header cannot be removed.
FIGURE 43. Group Setup Tab Users are assigned to groups. These users then have permissions granted to their group. Do not assign permissions directly to an individual user. On this tab, you can reinstate users who lock themselves out of the system at the System Login tab. This tab contains the following: Group Profile - The following user groups are predefined. • Administrator • Supervisor • Service • Technician2 • Technician1 • Reviewer The user will belong to the group you select.
The Allowed check box next to the desired permission in the Features section enables the selected group to perform that task. The Signature Required check box next to the desired permission requires a digital signature whenever a user in the selected group performs that task. Clear Allow and select Signature Required to require the electronic signature of another user whose account is configured to allow the action.
Lot and Std/Ctrl Kit Management • Create Std/Ctrl Kit and Lots • Edit Std/Ctrl Kit and Lots • Delete Std/Ctrl Kit and Lots Import and Export Data • Export Batch, Protocol, Std/Ctrl Kit/Lot Files • Import Batch, Protocol, Std/Ctrl Kit/Lot Files Archiving • Backup/Restore • Import/Archive When you perform an action that requires an electronic signature, the Electronic Signature dialog box opens. The user ID autopopulates. Type your password and any comments.
Create User Account Screen Use this tab to set the options for a new user. Any user with access to this tab can assign rights to any and all groups. FIGURE 45. Create User Account Screen This tab contains the following: User ID - Type a user ID in this box. The user ID is not case-sensitive. You can change the required number of characters on the User Setup tab. Once you create and delete a user ID, you cannot use that user ID again in the future. User - Type the user’s name.
Global User Settings - This section contains the following: • Password Expiration - Type a length of time in days. The default is 180 days. • Unsuccessful Login Attempts - The default number of allowed unsuccessful logins is three attempts. • Automatic Logoff - (seconds) - How many seconds pass until an automatic logoff. • Minimum User ID Length - The default minimum User ID length is six characters. • Minimum Password Length - The default minimum password length is six characters.
• Allow running a batch with expired reagents - Allows batches to run with expired reagents. • Allow running a batch if XY temperature is not in range - Allows batches to run if the XY temperature is not in range. • Allow running a batch if the instrument is not calibrated or verified - Allows batches to run if the instrument is not calibrated. • Use Weighting as default option for quantitative analysis - Sets the weighting for quantitative analysis to default to ON for new protocols.
FIGURE 47. Alert Options Tab Alert Options - Offers three check boxes for each alert - Dialog, Email, and Sound. This section displays the following events: • Locked User - The user is locked out of the login. • Batch Complete - The batch is finished. • Warmup Complete - The laser is warmed up. • Heater In Range - The plate heater is warmed to the designated range. • Scheduled Maintenance - A scheduled maintenance routine is now due.
Enable or disable email notification by selecting or clearing the Email Active check box. Contact your network administrator to determine the information needed in the Mail Server Host, From Email Address, From Email Password, and Mail Server Port boxes, and if you need to select the Enable SSL (Secure Sockets Layer) check box. The Test button tests the email settings. Select Apply to initiate the settings you’ve selected, or click OK to use the settings and exit the dialog box.
CSV and Batch Export Options Automatically export results CSV file when batch is complete - Automatically exports the .CSV file when the system finishes analyzing the batch. This allows running programs on exported data without having to manually start the export. Automatically export batch when batch is complete - Exports batch information automatically when a batch has completed. Maximum number of data columns in CSV file - Sets the number of columns into which your CSV output file will be organized.
FIGURE 49. Archive Tab This tab contains the following: Archive - Use this section to define archive locations for specific file types. • File Type - Choose from Protocols, Batches, Std/Ctrl Kits, Lots, Patient Samples, or Log. The selected information appears in the list below the Archive Folder path. • Keep data after archive - Retains data instead of deleting after you archive it.
Import - Select a file type to import in the File Type list and click Import to import all files of that type from the archive. The imported files will then display in the list below the Archive Folder path. Archive - Select a file type in the File Type list, and then select a file in the list below the Archive Folder path and click Archive to archive the file. If you have selected Keep data after archive, the file will still display in the list, otherwise it will no longer be displayed.
License - Opens the Change License dialog box. • Type the file path for the license file in the License File box, or use the Browse button to display the Open License dialog box and navigate to the file. You can also update the xPONENT® license status by manually typing the license code in the License Code box. It is not necessary to update both the license file and the license code. Updating either will serve to update the license.
Report Options Tab Use this tab to change the look of reports generated by the system. FIGURE 52. Report Options Tab • Company - Type the company name you wish to display on reports. • Info - Type any information you wish to display below the company name. • Import Logo - Displays the Open dialog box and selects the file you wish to use for the logo at the top of reports. • Clear Logo - Returns to the default logo. • Cancel - Cancels changes. • Save - Saves changes.
xPONENT® 3.1 Rev.
Chapter 3: Using the Software This chapter describes the steps needed to successfully use the xPONENT software. Starting the Software When you turn on the computer for your Luminex system, xPONENT® should launch automatically. If it does not, click the Luminex xPONENT icon on the PC desktop, or click Start > Programs > Luminex > xPONENT > Luminex xPONENT. If you have a trial license, a dialog box opens. Click OK to close the dialog box.
Setting Administrative Options Click Admin on the toolbar to display the tabs used to set administrative options. System Setup You must have xPONENT administrative privileges to perform System Setup tasks. Application Settings Use Application Settings to define how the software runs and displays. 1. Select the options you want to enable in the software. For a description of each option, see the Application Settings section. 2. Click Save.
5. Type the command line parameter for what parameters you want xPONENT to use with the external analysis program. If the information is supplied with the external analysis program documentation, use that information. Otherwise, you can type the following parameters built into xPONENT, in any order: • #c - Output.csv, full file path. • #p - Protocol name. • #b - Batch name. • #u - Logged in user name. To keep the default command line settings, leave Command Line Parameters blank. 6.
Group Setup You must have xPONENT administrative privileges and be using the Secure package of the software to perform group setup tasks. Additionally, you must have the 21 CFR Part 11 package to require electronic signatures. 1. In the Group Profile list, click the group profile you want to set up. 2. In the Group Features list, click which group features you want to set for the group profile you selected. 3.
Editing User Permissions In the Users list, click the user ID, then click Edit User. In the Edit User Account screen, edit the desired information, then click Save. Restoring Account Status If users attempt to log in unsuccessfully more than the number of times allowed, they will be locked out. In the User list, click the user ID, then click Edit User. Clear the Account status: Locked check box, then click Save.
3. In the Test Sort Order box, select the method that you want to use to sort the tests in the protocols. 4. Click Save. Archive Use the settings on the Archive tab to set options for backing up, exporting and restoring files. To archive a file: 1. In the File Type list, click the file type you want to archive. 2. Click the files you want to archive in the list below the Archive Folder box (this list displays all files of the selected file type in the internal database). 3.
1. Click Restore. A Warning dialog box displays. CAUTION: All data and settings will be overwritten, and xPONENT will restart once the restore task completes. 2. Click OK to continue restoring the system. The File Dialog box opens. The most recent backup file displays in the File Name box. To use a different backup file, click Browse to navigate to a different file. 3. Click OK to start the restore. A progress bar displays, followed by a Warning dialog box stating that xPONENT needs to be restarted. 4.
1. Type a company name in the Company box, and any additional information in the Info: box. 2. Click Import Logo and then Open after navigating to the desired file to import a graphic for the report header. 3. Click Clear Logo to remove the logo. 4. Click Save. Adjusting the Sample Probe Adjust the sample probe to ensure that it drops far enough into the well to acquire sample without impacting the plate. NOTE: Ensure that there is no liquid in the wells or reservoirs.
11. Tighten the probe adjustment screw. NOTE: Use caution not to move the probe up accidently when tightening the probe adjustment screw. 12. Click Move Probe Up. 13. Replace the plastic shield that covers the sample probe area. 14. Click Eject to eject the plate holder. Remove the alignment tools from the plate. System Initialization xPONENT® Software contains a pre-defined startup routine to prepare the analyzer for data acquisition.
Run System Initialization 1. On the Home page, click System Initialization. The Auto Maint page opens. 2. Select the performance verification kit from the Performance Verification kit list at the right of the tab. 3. Select the calibration kit from the Calibration Kit list. 4. Vortex the xMAP calibrator, verification, and fluidics containers to ensure homogeneity. Do not dilute xMAP calibrator or verification agents. 5. Click Eject on the status bar, then place the microtiter plate on the plate holder. 6.
5. Type the kit name for the exported kit into the File Name box. 6. Click Save. The dialog box closes. Delete CAL and VER Kit Information 1. Open the Maintenance page, then open the Lot Management tab. 2. In the Active Reagents section, select the kit you want to delete from the Calibration Kit or Performance Verification Kit lists. 3. Click Delete Kit. CAUTION: There is no confirmation dialog box when you delete a kit. Create Calibration Reports 1. Open the Results page, then open the Reports tab. 2.
NOTE: Samples can also be added via a LIS if your software is licensed for LIS. Edit Sample Lists 1. Open the Samples page. 2. In the Samples Lists section, choose the protocol you want to edit, then click Details. The Edit Samples tab opens. NOTE: If the number in the Number of Samples column is 0, there are no samples attached to the protocol. 3. Click a sample, then use the Move arrows to move it up or down in the sample list, changing the order in which they will be acquired. 4.
Create a New Batch from an existing Protocol Read the instructions provided with the assay kit you are using. 1. Open the Batches page. 2. Click Create a New Batch from an existing Protocol. 3. Type the batch name in the Batch Name box. 4. Type a description about the batch in the Enter Optional Description box. 5. Click a protocol you wish to use in the Select a Protocol list. 6. Click Next. If the protocol uses standards, controls, or both, the next tab that appears is the Stds & Ctrls tab.
9. To change the Default analysis, click Change. The Analysis Settings dialog box opens. 10. Select the analysis method from the Method list. Click OK to change the default analysis for analytes to be selected. Click Apply to All Analytes to apply the selection to all analytes. The Analysis dialog box closes. 11. Type the units of measurement you want to use in the Units box. If you click Apply All, this choice is applied to all analytes.
plate direction) as long as space remains on the plate. You can also select a well first, which places the next batch in your chosen location. If space limitations create an overlap, an error message appears. Results for each batch are saved as individual batch files. For more information, see the Create a Multi-Batch section. 1. Open the Batches page. 2. Click Create a New Multi-Batch. The New MultiBatch tab and Select Pending Batch dialog box open simultaneously. 3.
3. Click Browse. The Select File dialog box opens. 4. Navigate to the location in which you want to save the file, then click Save. Click OK in the Export Batch dialog box. NOTE: When exporting a large batch and including the LXP files, such as a batch with hundreds of wells or hundreds of analytes, the export process may take ten minutes or more. Delete Batch You can only delete unprocessed batches. Batches are deleted from the Open Batch list and moved to the Open Incomplete Batch list. 1.
You can analyze an acquired batch using the analysis features of Qualitative, Quantitative, and Allele Call algorithms. Qualitative Analysis - Determines results as either positive or negative, reactive or nonreactive, and so on. The system is flexible in defining custom result ranges, such as negative, low positive, and high positive. Quantitative Analysis - Determines the sample concentrations from standard curves using regression methods, such as 4P or 5P logistic curve fitting.
9. On the Stds & Ctrls tab, type the appropriate information in the Standard Information and Control Information sections. The number of Standards and/or Controls in these sections is defined on the Settings tab in the Analysis Settings section. If your batch uses controls, enter the appropriate values for Expected Values. Click Low Value from the Show list, and enter the low value for each analyte. Click High Value from the Show list, and enter the high value for each analyte.
Invalidate Standards and Controls Your xPONENT system administrator must give you privileges to invalidate standards if you are using the Secure xPONENT package. You can invalidate or remove a standard if doing so improves the curve fit. CAUTION: Observe caution when invalidating standards. Doing so greatly affects the curve fit and subsequent sample results. For further instruction on assay standard curves and the appropriateness of invalidating or removing standards, contact the assay kit manufacturer.
View and Print Reports The Luminex xPONENT software can format your batch or multi-batch results in a variety of export formats and provide different types of information in different types of reports. Three types of reports are available. See Reports Tab for a description of available reports. NOTE: Install the printer before initiating the Print command. 1. Open the Results page, then open the Reports tab. 2. In the Report list, click the report you want to view. 3.
Creating a Qualitative Assay Protocol You define each Qualitative assay protocol using a single kit. The kit must contain one standard. The standard is assigned a “Quali” value for each test. Controls are optional, but are recommended for judging the acceptability of batch results. To create a Qualitative Assay Protocol: 1. Open the Protocols page, then open the Protocols tab. Click Create New Protocol. The Settings tab opens. 2. In the Name box, type the name of the protocol. 3.
13. Click Next. The Plate Layout tab opens. • To add well commands, highlight the appropriate wells and mark them as unknown, standard, control, background, or wash. You can also delete commands that you’ve added, and change the starting location on the plate. If you want to run in replicate, change the Replicate Count to the appropriate number and the grouping to your preferred grouping method. • To add maintenance commands, choose a command from the list.
9. Check Mark as Intra-Well Normalization Bead if you want to use a normalization bead. The normalization bead is a microsphere set that is included in the assay as an internal control. It controls for sample variation and can be used to normalize data between samples in a run. An analyte used as a normalization bead will appear blue in the analyte grid. 10. Type a name for the new formula in the Formula Name box. 11. Click Save Formula. The new formula displays in the Formulas list. 12.
18. Select a weight type in the Weight Type list (Weight Type may not display, depending on the analysis method selected in the Method list). 19. Check Mark as Intra-Well Normalization Bead if you want to use the analyte as a normalization bead. The normalization bead is a microsphere set that is included in the assay as an internal control. It controls for sample variation and can be used to normalize data between samples in a run. 20. Check Use Threshold Ranges if you want to use a range for the analysis.
27. Click Next. The Plate Layout tab opens. • To add well commands, highlight the appropriate wells and mark them as unknown, standard, control, background, or wash. You can also delete commands that you’ve added, and change the starting location on the plate. If you wish to run in replicate, change the Replicate Count to the appropriate number and the Grouping to your preferred grouping method. • To add maintenance commands, choose a command from the list.
NOTE: For more information about the commands and settings on the Plate Layout tab, see the Plate Layout Tab. Editing Quantitative Analysis Settings To edit quantitative analysis settings: 1. Open the Protocols page, then open the Protocols tab. Select a protocol and click Edit. 2. From the Analytes tab, click an analyte’s Analysis field to open the Analysis Settings dialog box. 3. From the Method list, click No Analysis, Cubic Spline, Linear Fit, Logistic 4P, or Logistic 5P. 4.
9. Click the desired analyte (bead ID) in the numbered grid. Information about the analyte appears in a list on the right side of the grid. For an allele call analysis, you must select a group of two, three, or four analytes. 10. Click Group to group the analytes for the allele call. The grouped analytes display in a list to the right. Select more analytes and then click Group again if you want to add another group to the analysis. 11.
4. Click Apply Std/Ctrl Kit to associate a kit with the protocol. If you are not using a kit, type the appropriate Standard and Controls information in the Assay Standard Information and Assay Control Information sections. 5. Click Save. Delete Protocol 1. Open the Protocols page, then open the Protocols tab. 2. Select a protocol. 3. Click Delete. The Delete Protocol dialog box opens. 4. Click Yes. Export Protocol 1. Open the Protocols page, then open the Protocols tab. 2. Select a protocol. 3.
4. Click Apply Std Lot if you wish to apply a standard lot. The Select Lot dialog box opens. Click a lot and select OK. 5. Click Apply Ctrl Lot to apply a control lot. The Select Lot dialog box opens. Click a lot and click OK. 6. Alternatively, type the appropriate information in the Assay Standard Information and Assay Control Information sections. The number of standards and/or controls in these sections is defined in the protocol.
Edit Lot 1. Open the Protocols page, then open the Protocols tab. Click the Stds & Ctrls tab. 2. In the Installed Kits And Lots section, select a lot and then click Edit. The Std/Ctrl Details tab opens. Change the lot information as appropriate. Performing System Maintenance Performing Individual Maintenance Commands Open the Maintenance page, then click the Auto Maint tab to display the automated maintenance options.
5. Click Save As to save the new routine. The Save Routine dialog box opens. 6. Type the routine name in the Routine Name box, then click OK. Edit Routine You can also edit routines. 1. Open the Maintenance page, then open the Cmds & Routines tab. Click the routine that you want to edit from the Routine Name list. 2. Click a command in the Command Sequence list that you wish to edit. Click Clear to delete the command, or click in a different well in the plate image to change the location of that command.
NOTE: If you haven’t previously saved a routine, the Export button will not display. Support Utility Use the options on the Support Utility tab to create a support file to send to Luminex Technical Support. 1. Type your name in the Name box. 2. Type your company name in the Company box. 3. Type your phone number in the Phone box. 4. Type your e-mail address in the Email box. 5. In the Comment box, type a detailed description about the problem you are experiencing. 6.
Technical Support Luminex Technical Support representatives are ready to help you, particularly when the system or software cause any questions or problems. If the question or problem relates to materials from the assay kit, you should contact the kit provider directly. Luminex Technical Support is available to users in the U.S. and Canada by calling 1-877-785-BEAD (2323). Users outside of the U.S. and Canada can contact us at +1 512-381-4397. Inquiries may also be sent by email to support@luminexcorp.com.
xPONENT® 3.1 Rev.
Appendix A: Converting IS 2.3 .idt Files to xPONENT 3.1 .lxt Files Overview: Converting IS 2.3 .idt Files to xPONENT 3.1 .lxt Files When upgrading from the IS 2.3 system to the xPONENT® 3.1 system, the IS 2.3 (template) .idt files become incompatible with the next generation xPONENT 3.1 .lxt (protocol) file format. The following steps provide assistance in converting the IS 2.3 .idt files to xPONENT .lxt files for use with the xPONENT 3.1 system.
Edit the IS 2.3 as .idt Files (Template) NOTE: For additional information about .idt file editing, see the Overview: Converting IS 2.3 .idt Files to xPONENT 3.1 .lxt Files section. From the IS 2.3 system, edit a used template by perform the following steps: 1. From the Acq. Detail tab, click Edit Template on the Developer Workbench toolbar. An Open Template dialog box opens. NOTE: Associated batches will display a "lock" icon in the ID column. FIGURE 53. Open Template Dialog Box 2.
Exporting Templates from IS 2.3 as .idt Files NOTE: Before starting the export process, make note of the files that are to be exported to the xPONENT 3.1 system. 1. Click the Acq. Detail tab. FIGURE 55. Acq. Detail Tab 2. Click the Export Template icon. FIGURE 56. Export Template Icon 3. Click to select the template to be converted and then click the Export button. FIGURE 57. Select Template to Export Converting IS 2.3 .idt Files to xPONENT 3.1 .
4. Once the template has been successfully exported, make a note of the directory where the exported file was saved to and then click the OK button. FIGURE 58. Template Export Confirmation 5. Browse to the directory where the template was been exported to as an .idt file (C: \Program Files\Luminex\Luminex 100 IS\ExportedTemplates). FIGURE 59. Template Export Location 6. Copy the .idt file from the IS 2.3 computer to the xPONENT 3.1 computer. NOTE: The xPONENT 3.
1. From the system taskbar, click Start, All Programs, Luminex, xCONVERT, then Luminex xCONVERT. FIGURE 60. Launch the xCONVERT Utility The xCONVERT Utility window opens. 2. Click the ... button to the right of the Source file: field. FIGURE 61. xCONVERT Utility Source File Converting IS 2.3 .idt Files to xPONENT 3.1 .
3. Browse to the directory containing the exported IS 2.3 template (.idt) file and click to select the template file to be converted. Click the Open button. FIGURE 62. Select Template to Convert xCONVERT will automatically detect the format of the file to verify that it can be converted. Once detection is complete, the last line in the status window will show that the file is an IS 2.3 template file. FIGURE 63. xPONENT® 3.1 Rev. 2 128 IS 2.
4. Click the … button to the right of the Destination Folder: field to change the directory where the converted file will be saved to. FIGURE 64. Change Destination Folder for Conversion 5. Click to select the folder to which the new file will be saved to and then click the OK button. FIGURE 65. Browse to the Folder for Storage Converting IS 2.3 .idt Files to xPONENT 3.1 .
6. Click the Convert button to begin the file conversion. FIGURE 66. Begin the Conversion Process 7. Once complete, the status window confirms the file conversion. Click the OK button in the Success dialog box FIGURE 67. File Conversion Confirmation xPONENT 3.1 on the xPONENT 3.1 Computer Importing Converted Protocols into xPONENT NOTE: Step 4 of this procedure begins on the xPONENT 3.1 system. xPONENT® 3.1 Rev.
1. From within the xPONENT 3.1 program, click the Protocols tab from the tab bar at the top of the window, click the Protocols tab on the left side of the window, then click the Import button. FIGURE 68. Protocols Tabs 2. Navigate to the folder containing the newly-converted protocol (.lxt file) then click to select the file and click the Open button. FIGURE 69. New Protocol 3. Once the import has succeeded, the new protocol is shown in the Installed Protocols list.
FIGURE 70. Import Protocol File Confirmation Once the import is complete, the newly converted protocol is ready to be used for batch acquisition in the xPONENT 3.1 system. xPONENT® 3.1 Rev.
