Owner manual

x
M
89-3
0
Mic
r
3
4
5
6
7
A
ct
i
8
9
1
1
1
1
1
1
Inc
u
1
Mic
r
1
1
1
Co
u
2
2
2
Inc
u
2
Mic
r
2
2
2
2
2
2
M
AP
®
A
0
000-00-361
r
osphere
W
3
. 
R
a. 1
b. 4
4
. 
D
5
. W
A
SH S
T
a.

b.

c.

d.

6
. 
R
7
. 
R
i
vation:
8
. 
A
9
. 
V
1
0. 
V
1
1. 
A
1
2. 
A
1
3.  I
n
1
4. 
A
1
5. 
V
u
bation #1:
1
6.  S
a. I
n
b. I
n
r
osphere
W
1
7. WASH S
T
a.

b.

c.

d.

1
8. 
R
1
9. 
R
u
pling:
2
0. 
A
2
1. 
A
2
2. 
V
u
bation #2:
2
3.  S
a.
R
b.
R
r
osphere
W
2
4. WASH S
T
a.

b.

c.

d.

2
5. 
R
2
6. 
R
2
7. 
A
2
8. 
V
2
9.  P
bC Kit
Rev.
A
W
ash #1:
R
esuspend th
e
mL – vortex
mL – rotate
f
D
ispense the
d
T
EP – use on

 Place

 Remo
v


A
dd 5
0

 Vorte
x
R
epeat WAS
H
R
emove liquid
A
dd Activatio
n
V
ortex and so
n
V
ortex Sulfo-
N
A
dd Sulfo-NH
A
dd 250 μL of
n
vert EDC vi
a
A
dd EDC solu
t
V
ortex and so
n
hield from lig
n
cubation sta
r
n
cubation en
d
W
ash #2:
T
EP – use on

 Place

 Remo
v


A
dd 5
0

 Vorte
x
R
epeat WAS
H
R
emove liquid
A
dd Activatio
n
A
dd Antibod
y
V
ortex the rea
c
hield from lig
R
otation start
t
R
otation end t
i
W
ash #3:
T
EP – use on

 Place

 Remo
v


A
dd 5
0

 Vorte
x
R
epeat WAS
H
R
emove liquid
A
dd 1mL of W
V
ortex and so
n
rotect from li
g
Quick
e
stock Bead
and sonicate
f
or 15 minute
s
d
esired volu
m
e disposable
reaction tub
e
v
e supernata
0
0 μL of Acti
v
x
and sonicat
e
H
STEP, for a
from
Beads
n
Buffer to r
e
n
icate reactio
N
HS tube for
S to reaction
Activation
B
a
l and vortex
f
t
ion to reacti
o
n
icate reactio
ht and rotate
r
t time:
d
time:
e disposable
reaction tub
e
v
e supernata
0
0 μL of Acti
v
x
and sonicat
e
H
STEP, for a
from
Beads
n
Buffer to t
h
y
to the reacti
o
c
tion tube for
ht and rotate
t
ime:
i
me:
e disposable
reaction tub
e
v
e supernata
0
0 μL of Wa
s
x
and sonicat
e
H
STEP, for a
from
Beads
ash Buffer t
o
n
icate reactio
g
ht and store
Guide
s
for 10 secon
s
at 15-30 rp
m
m
e of Beads
(
pipette per r
e
e
into magnet
i
nt with transf
e
v
ation Buffe
e
reaction tu
b
total of two
w
w/ magnetic
e
action tube
(
n tube for 10
10 seconds
tube (from
S
t
B
uffer to 10
m
f
or 10-12 sec
o
o
n tube (from
n tube for 10
the reaction
t
pipette per r
e
e
into magnet
i
nt with transf
e
v
ation Buffe
e
reaction tu
b
total of three
w/ magnetic
h
e reaction tu
o
n tube (from
10 seconds
the reaction
t
pipette per r
e
e
into magnet
i
nt with transf
e
s
h Buffer int
o
e
reaction tu
b
total of three
w/ magnetic
o
the reactio
n
n tube for 10
at 2-8 ºC un
t
ds
m
(
from Step 2
a
e
action tube
i
c separator (
e
r pipette
r into reactio
n
b
e for 10 sec
o
w
ashes
separator (1-
(
from Step 2
b
seconds
t
ep 2c )
m
g vial of ED
C
o
nds
Step 2d )
seconds
t
ube for 20 ±
e
action tube
i
c separator (
e
r pipette
r into reactio
n
b
e for 10 sec
o
washes
separator (1-
be (from
Ste
p
Step 2e )
t
ube for 2 ho
u
e
action tube
i
c separator (
e
r pipette
o
reaction tub
e
b
e for 10 sec
o
washes
separator (1-
n
tube
seconds
t
il needed
a
) to the reac
t
1-2 min)
n
tube
o
nds
2 min) and d
i
b
)
C
.
2 minutes @
1-2 min)
n
tube
o
nds
2 min) and d
i
p
2f )
u
rs ± 5 minut
e
1-2 min)
e
o
nds
2 min) and d
i
t
ion tube
i
sposable pip
@
15-30 rpm
i
sposable pip
e
s @ 15-30
r
i
sposable pip
ette
ette
r
pm
ette
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