Owner manual

x
M
89-3
0
This
Q
inten
d
Rea
g
1
Rea
g
2

M
AP
®
A
0
000-00-361
Q
uick Guide is a
d
ed to replace th
e
g
ent Prep:
1
. 
A
g
ent Calcu
l
2
. 
C
a. Beads
Desire
d
Conce
n
b. Activa
t
i. 4
0
ii. 4
8
c. Sulfo-
N
i. 5
0
ii. 1
0
d. EDC f
o
i. 5
0
ii. 1
0
e. Antib
o
D
e
D
e
S
t
f. Activa
t
Total
r
i. 1
0
ii. 5
0
Volum
e
Volum
e
bC Kit
Rev.
A
checklist of all o
f
e
kit
u
ser manu
a
A
llow kit to eq
u
l
ations:
C
alculate the
v
for coupling rea
d
number of Be
a
n
tration of stock
Volume o
f
Volume o
f
t
ion Buffer for
A
0
0 μL for reactio
n
8
0 μL for reactio
n
N
HS for Activati
o
0
μL for reaction
s
0
μL for reaction
s
o
r Activation ste
p
0
μL for reaction
s
0
μL for reaction
s
o
dy for coupling
s
e
sired number o
e
sired Antibod
y
t
ock Antibody c
o
Volume of
A
Volume of
A
t
ion Buffer for
c
r
eaction volum
e
0
00 μL for reacti
o
0
0 μL for reactio
n
e
of Activation B
u
e
of Activation B
u
Quick
f
the steps for p
e
a
l (89-00002-00-
3
u
ilibrate to ro
o
v
olumes requ
c
tion:
a
ds to couple:
vial:
f
stock need
e
f
stock need
e
A
ctivation step:
n
s of more than
5
n
s of 5x10
6
Bea
d
o
n step:
s
of more than 5
x
s
of 5x10
6
Bead
s
p
:
s
of more than 5
x
s
of 5x10
6
Bead
s
s
tep:
f Beads to coup
y
concentration:
o
ncentration:

A
b needed =

A
b needed =
c
oupling step:
e
:
o
ns of more tha
n
n
s of 5x10
6
Bea
d
u
ffer needed = t
o
u
ffer needed =

Guide
e
rforming one co
3
19). Please re
fe
o
m temperat
u
ired for each


# of be
e
d =
conc
o


___
_
e
d =
_
____
_
5
x10
6
Beads
d
s or less
x
10
6
Beads
s
or less
x
10
6
Beads
s
or less
le:

# of beads to
S
t


6
_
___ 10 b
e
μg
_
___

µL
n
5x10
6
Beads
d
s or less
o
tal reaction vo
upling reaction
u
fe
r to the user m
a
u
re for 20 to
3
reagent:
x10
6
x10
6
B

a
ds to coupl
e
o
f stock vial


_
__beads
beads
_
_
mL
x10
6
µg/1
x
µg/m
L

couple Desir
e
t
ock Ab conc


6
___
μ
e
ads
110 b
1 m L
mL
1000 μL









lume – volume
o
µL -
u
sing the xMAP
A
a
nual for compl
e
3
0 minutes
B
eads/mL

e
x
10
6
Beads
L

e
d Ab conc
μ
g
b
eads






o
f Antibody
µL
A
ntibody Coupli
n
e
te instructions,
w
V
o
l
th
e
V
ol
u
nee
d
V
ol
u
acti
v
V
ol
u
acti
v
V
ol
u
cou
p
V
ol
u
nee
d
n
g kit (40-50016
)
w
arnings, and p
re
l
ume of Bead s
t
e
coupling reac
t
u
me of Activati
o
d
ed for activati
o
u
me of Sulfo-N
H
v
ation (Step 11
)
u
me of EDC ne
e
v
ation (Step 14
)
u
me of Antibod
y
p
ling (Step 21):
u
me of Activati
o
d
ed for couplin
g
_
Page 1 of
2
)
and is not
re
cautions.
t
ock needed fo
r
t
ion (Step 4):
m
o
n Buffer
o
n (Step 8):
µ
H
S needed for
)
:
µ
e
ded for
)
:
µ
y
needed for
µ
o
n Buffer
g
(Step 20):
_
___________u
2
r
L
L
L
L
L
L