® Luminex Salmonella Analysis Tool User Instructions
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Table of Contents Salmonella Analysis Tool User Instructions .................................................................................................1 Purpose .................................................................................................................................................1 Requirements ........................................................................................................................................1 Application Installation ....................
Luminex® Salmonella Analysis Tool iv
Salmonella Analysis Tool User Instructions Purpose The Salmonella Analysis Tool is intended as an aid for analysis of data generated with the Luminex® xMAP® Salmonella Serotyping Assay. The analysis produced by this application is used to determine the possible Salmonella serotypes based on the Kauffman-White algorithm for Salmonella serotyping and user input of positive antigen/probe data. NOTE: This application is for research use only. Performance characteristics have not been evaluated.
Application Overview The Salmonella Analysis Tool displays three drop-down boxes for selection of O group antigens (1) and Phase 1 and 2 of H antigens (2). A fourth drop-down box allows you to select a specific Salmonella serotype (3). A Search box is also available as an alternative for data input (4). Upon initial launch of the application, all possible Salmonella serotypes per Kauffman-White scheme are displayed by default. The list displays 10 entries at a time.
2. Select the applicable phase 1 and 2 antigens from the H Group drop-down boxes. The analysis tool further analyzes the data and only displays the serotypes associated with the selected H antigens in addition to the previously selected O Group antigen(s). The selected antigens for O and H display in blue below the drop-down boxes. 3. To deselect an antigen, hover over the entry with the mouse. An “X” displays next to the entry and the font changes to red. Click on the “X” to deselect the antigen.
assay. Use the same approach to narrow down the serotype output for samples that do not produce a positive antigen result for the H Group targets covered by the assay. Some serotypes contain antigens that are not included in the current Luminex® xMAP® Salmonella Serotyping Assay. If partial information is obtained from the assay for these serotypes, the Analysis Tool can be used to produce a list of potential serotypes, which reduces the number of other tests needed to generate a complete serotype identity.
