User Manual (supplied by BioRad) Owner's manual

ManualsBrandsLuminex ManualsEquipmentBioPlex XYP, LX200

5.2 Troubleshooting Guide for Vacuum Manifold

Message/Problem Causes Solution

No Flow/no vacuum. Lid on plate Remove lid.

All wells not wet, or Wet unused wells with Milli-Q

unused wells not covered water. (You can reuse these wells

or sealed later.) Or, tape the unused wells

with sealing tape. If you need to seal

partial rows or columns, seal the

unused rows or columns with tape

and leave the adjacent unused row

partially sealed. Then wet with buffer

since it is difficult to make seals at the

row edge.

Poor alignment of plates Align plates.

with gasket

Vacuum trap filled Empty trap.

Filter on pump clogged Replace filter.

Pump not turned on Turn on pump.

Manifold ON/OFF valve in Turn to ON position.

OFF position

Manifold pressure gauge Turn up to higher value.

turned to the lowest value

Damaged gasket Replace gasket.

Gauge plug missing Replace gauge plug in side of

manifold ring using hex key wrench.

Wells do not empty at the Lid on plate Remove lid.

same time/uneven flow.

Vacuum line turned off or Clear line and repeat.

clogged

Debris in sample Remove cellular debris prior to

adding to wells.

Bad plate-to-vacuum To ensure a good plate-to-manifold

manifold seal seal, put pressure on corners of

plate.

Too many beads Check to ensure that proper bead

concentration was used.

Leakage during incubation. High surfactant Lower the concentration.

concentration

Failure to blot under-drain Blot under-drain.

after filtration and before

incubation

Under-drain contacting Place plate on smooth, flat surface

surface so nothing touches the underdrain

spouts.

Absorbent material Place on flat, nonabsorbent

contacting under-drain material (such as lid).

Excessive agitation or Mix using orbital table at a lower

vibration volume (maximum 200 µl on shaker,

340 µl without a shaker) or use lower

speed.