User Manual (supplied by BioRad) Owner's manual

Bio-Plex Manager Incorrect bead regions Compare bead regions in the assay
has detected a problem with selected in the protocol with those selected in the protocol.
bead selection.
Incorrect regions selected Verify regions chosen during assay
when preparing the assay preparation.
Too few beads in the assay Verify that the correct number of
beads in one or more regions
were used.
Bio-Plex Manager has Most likely:
detected a problem with
assignment of beads into Calibration performed before Perform 30 min Warm Up and
regions. the array reader was warmed recalibrate.
up
Improper calibration Check that the target values of the
CAL beads match values entered in
the software, then recalibrate.
Microbubbles present in Perform Remove Bubbles.
cuvette.
Less likely:
Calibration beads are Recalibrate with new Cal1 beads.
photobleached (do not
expose to light for more
than 1 hr)
Array reader was calibrated Clean MCV plate and recalibrate.
with a dirty MCV plate
Misalignment of optics Perform Optical Validation.
Contact Technical Support if values
are not within range.
Bio-Plex Manager has Clumped beads present Vortex plate at 900 rpm for 1 min.
detected a problem with
aggregated beads. Sheath reservoir is empty Refill sheath reservoir. Perform
Start Up.
Waste reservoir is Empty waste and reconnect.
overfilled
Problem with doublet Use default DD gate setting. Run
discrimination (DD) Classify Validation to check DD
efficiency.
Incompatible suspension Check hardware manual for buffer
buffer used compatibility.
Check Link in status bar of Array reader or microplate Turn on array reader and microplate
software. platform not turned on platform.
Software is not communicating Close and restart Bio-Plex Manager
with array reader software.
Cables from computer to Check cables for proper connections.
array reader or microplate
platform are loose/not
connected
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Message/Problem Causes Cause Solution