User Manual (supplied by BioRad) Owner's manual

Section 4

Care and Maintenance

Table 6. Summary of Care and Maintenance.

Daily Startup, calibrate, wash between plates, shut down

Weekly Sonicate needle, unclog, check for leaks

Monthly Validation, clean exterior surface

6 months Replace syringe seal, clean ventilation filter

Yearly Replace sheath filter, replace air intake filter

Note

: Two long needles are included with the Bio-Plex

200 system. While one is being

cleaned, the other can be installed to prevent any downtime from this weekly maintenance.

Proper maintenance and cleaning should be performed in order to preserve the longevity and

reliability of the system.

Regularly As Required

Preventing clogs in the The Start Up, Shut Down, and Wash Between Plates

fluidics system procedures must be strictly followed to prevent

clogging of the fluidics system.

Sheath fluid and waste fluids Replace the sheath fluid and empty the waste container

as required. You must manually monitor the waste

container level. Release the instrument’s pressure by

removing the lid from the sheath fluid container. Add

sheath fluid, filling the sheath fluid container to just

below the air intake. Discard waste fluid by appropriate

means. After closing containers, remove air from the

lines by performing a Start Up.

One must refill sheath and empty the waste after two

full plates are run.

Run a Wash Between Plates function after each plate

is run.

Check for leaks Open all instrument doors and visually inspect for

leaks. If a leak or spill is observed, verify whether it is

coming from the sample needle assembly. Check to

see that the knurled tubing connector is tightened

properly (see Section 3.8). If this is not the source of

the leak, turn off all power to the system, disconnect all

power cords, and contact Bio-Rad Technical Support

for further information.

Sanitize Fill MCV plate with a solution of 10% household

bleach. Place MCV plate in microplate platform.

Choose Shut Down.