Lum/nex ® Luminex 100™ User Manual Version 1.
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Luminex 100 User Manual Version 1.7 Addendum This Addendum to the Luminex® 100TM User Manual Version 1.7 contains information that became available after the release of the manual. Some of the topics presented in this addendum were not published in the previous release; some topics replace information that exists in the previous manual. Please read through this addendum to familiarize yourself with the new content.
xMAP Technology Luminex 100 User Manual Version 1.7 Addendum Safety and Regulatory Symbols New safety and regulatory symbols have been added to the Luminex 100 system and manuals. Please become familiar with these symbols. These symbols describe warnings, cautions, and general information used in the operation of this instrument.
xMAP Technology Storing the Analyzer Luminex 100 User Manual Version 1.7 Addendum To prepare the system for storage: 1. Run a Sanitize command with 10% to 20% household bleach solution. 2. Run a Sanitize command with distilled water. 3. Run four Wash commands with distilled water. 4. Remove the sample probe from the instrument, flush with distilled water from the narrow end out through the larger end, replace it in the sample arm and wrap the end with Parafilm.
xMAP Technology Luminex 100 User Manual Version 1.7 Addendum To minimize the chance of acquiring air, be sure that the sample acquisition volume is at least 25 µL less than the total volume in the well. Cleaning the Analyzer Perform the following steps during Startup: 1. Warmup 2. Prime 3. Alcohol Flush with 70% isopropanol or 70% ethanol 4. Wash twice with distilled water Perform the following steps during Shutdown 1. Sanitize with 10% to 20% household bleach solution. 2.
xMAP Technology Luminex 100 User Manual Version 1.7 Addendum contacting Technical Support. You can move the line temporarily for cleaning and maintenance purposes. Sheath Delivery System Setup When adjusting the Luminex 100 regulator, turn it clockwise as far as it will go to the fully closed position, then turn it back 1/2 of a turn. Be careful not to force the regulator past the fully closed position. Do not move the Sheath Delivery (SD) system or the sheath line while the system is in use.
Luminex 100 User Manual Version 1.7 Addendum xMAP Technology Be sure the plate you are using is completely flat – warped plates can lead to incorrect probe height adjustment. In a 96-well microtiter plate where overall height is no more than 0.75 inches (19 mm), place the appropriate alignment tool in the plate: • • • • • Sheath Fluid 6 For a standard plate with flat-bottomed wells – stack two of the larger (5.08 mm diameter) alignment discs together and place them into the selected well.
