User Manual, Version 2.1

Luminex 100 IS User Manual Version 2.1 xMAP Technology
5 - 4 PN 89-00002-00-061 Rev. A
Accuracy and Precision • Sample uptake volume ± 5%
• Classification of microspheres > 80%
• Misclassification of microspheres < 0.5%
• Temperature control 0°C to +2°C of target
• Internal sample carry over < 0.9%
• Soluble background fluorescence emission at 575 nm
automatically subtracted from fluorescence intensity values
Sensitivity • Detect 1000 fluorochromes phycoerythrin (PE) per microsphere
• Reporter channel dynamic range: 3.5 decades
Capacity The specifications below reflect minimum capacity values:
• 10 GB hard drive
• Store data for up to 240,000 test results
• 1.4 MB 3½” floppy disk
• 100 MB read/write CD ROM
• Analyze multiple 96-well plates per batch
• Analyze multiple assay templates per plate
• Distinguish a minimum of 1 to a maximum of 100 unique xMAP
microsphere sets in a single sample
• Detect and distinguish surface reporter fluorescence emissions at
575 nm on the surface of 1-100 unique xMAP microspheres sets
in a single sample
• Reporter channel range: 3.5 decades of detection
• Sample core: 15 - 20 µm core at 1 µL/sec sample inject rate
• Maintain samples at a constant temperature from 35°C to 55°C
(95°F to 131°F)
• Automatic sampling from a 96-well plate
• Start sampling from any well position
• Sheath container and waste container hold enough volume to run
up to two 96-well plates between refills
• Microtiter plates with 96 wells must be compatible with the XYP
instrument plate holder. The following microtiter plate types are
compatible with the XYP instrument plate holder: flat bottom,
conical, round, filter bottom, half plates [overall height no more
than 0.75” (19 mm)], any color
• Microtiter plates with 96 wells must be compatible with XYP
instrument heater block temperature from 35°C to 55°C (95°F to
131°F) when performing heated assays and using the heater
block.