User Manual, Version 2.1

Luminex 100 IS User Manual Version 2.1 xMAP Technology
11 - 2 PN 89-00002-00-061 Rev. A
CL2 Refers to dyes embedded in the microsphere. Also see classification
channel.
classification channel A specific range of wavelengths in which light intensity is measured.
Includes the emission of a given classification dye. Classification
channels are abbreviated as CL1 and CL2.
control, assay Used to verify standards within the kit. Tells you that the curve or
thresholds are correct.
control, system Microspheres used to verify the calibration and optical integrity for
the analyzer.
cuvette Principal fluid pathway within the optics component of the system
through which the sample is read.
delta cal temperature The difference between the current temperature of the Doublet
Discriminator APD and its temperature at your last calibration. The
system displays this value on the Diagnostics tab within the software.
DD temperature The current temperature of the doublet discriminator avalanche photo
diode.
emission spectrum Wavelength range that an excited fluorochrome emits when its
electrons fall from a higher to a lower energy state. Expressed in
nanometers (nm).
event Occurs when the signal processor determines that a particle is being
observed.
excitation spectrum Wavelength range that excites a molecule’s electrons to a higher
energy state. Expressed in nanometers (nm).
fluorescence Light emission that occurs when the electrons of a fluorochrome
drop to a lower energy state.
fluorochrome A fluorescent molecule.
fluorophore See fluorochrome.
immunofluorescence A technique which uses a covalently linked fluorochrome-antibody
complex to detect or quantify a particular antigen.