Manual
xMAP Technology Troubleshooting
PN 89-00002-00-069 Rev. A 8 - 7
If you power the off, you must calibrate and verify the system
when the system properly connects.
Locked Out • Close all software applications other than the Luminex 100 IS
2.2 software.
Refill Sheath A warning displays in red under the temperature and pressure gauges
on the Run Batch tab.
• Refill the sheath container and select Resume.
Running: Sheath
Empty
• Replace or fill the sheath fluid container with sheath fluid. From
the Maintenance tab, click Prime 2 times.
Sample Error Messages The following error messages are displayed:
• On the Diagnostics tab in the Message Log
•In Reports
• On the Errors tab in a batch analysis.
Insufficient Bead
Count
• Do not select Cancel during sample acquisition. This causes an
error message to occur.
• Verify that you select the correct template.
• If applicable, verify that all of the tests (beads) were added to the
samples.
• Verify that the samples were properly mixed before they were
dispensed into the wells.
• Verify that you loaded the sample to the correct well on the
plate.
• Verify that the correct sample volume is added to the well.
• Verify that the samples were completely resuspended prior to
running on the analyzer.
• Remove and sonicate or flush the Luminex XYP instrument
Sample Probe to remove possible clogs.
• Verify that the Luminex XYP instrument Sample Probe is
correctly aligned.
• Complete the following analyzer functions from the Maintenance
Tab: Backflush, Drain, Alcohol Flush and Water Wash.
• Run the system xMAP control microspheres.
Note: Do not install
unapproved software
applications on the Luminex
100 IS 2.2 system.