User Manual

xMAP Technology Luminex xMAP Technology

PN 89-00002-00-062 Rev. A 3

Capacity The following specifications reflect minimum capacity values:

• 10 GB hard drive

• Store data for up to 240,000 test results

• 1.4 MB 3½” inch floppy disk

• 100 MB read/write CD ROM

• Analyze multiple 96-well plates per batch

• Analyze multiple assay templates per plate

• Distinguish a minimum of 1 to a maximum of 100 unique xMAP

microsphere sets in a single sample

• Detect and distinguish surface reporter fluorescence emissions at

575 nm on the surface of 1 - 100 unique xMAP microsphere sets

in a single sample

• Reporter channel range: 3.5 decades of detection

• Collect from 10 to 100,000 events per xMAP microsphere set

specified

• 2.5 x 10

microspheres/mL maximum bead concentration

• Sample uptake volumes from 20 to 200 µL

• Sample core: 15-20 µm core at 1 µL/sec. sample injection rate

• Sample timeout limit from 1 to 400 seconds

• Automatic sampling from a 96-well plate

• Start sampling from any well position

• Sheath container and waste container hold enough volume to run

up to two 96-well plates between refills

• 96-well microtiter plates types:

Must be compatible with XYP instrument plate holder: Flat

bottom, conical, round, filter bottom, half-well plates with

overall height no more than 0.75 inches (19 mm). Use black

opaque plates if possible to reduce photobleaching of beads.

• Microtiter plates with 96 wells must be compatible with XYP

instrument heater block temperature from 35°C to 55°C

(tolerance 0°C to ± 2°C) (95°F to 131°F) when performing

heated assays and using the heater block