User Manual

Luminex 100 IS Developer Guide to xMAP Technology Version 2.1 xMAP Technology
2 PN 89-00002-00-062 Rev. A
Performance
Specifications
Speed • Speed: greater than or equal to 933 MHz Pentium III processor
with 256 MB RAM
• USB cabling for fast data transfer
• Automatic transfer of assay templates and new reagent
information into the system using a 3½” inch floppy disk or large
capacity read/write CD
• Installation < 4 hours
• System calibration < 10 minutes
• System controls < 10 minutes
• Barcode reader entry of sample IDs
• Automatic post analysis
• Analyze one 96-well plate/hour depending on manufacturer’s kit
• Up to 100 xMAP microsphere sets per sample
• Sheath flowrate 90 µL/sec. ± 5 µL
• Sample injection rate into detector area 1 µL/sec. ± 0.05 µL
• System warmup 30 minutes: Systems that remain inactive for at
least four hours will require a warm-up to restart the lasers. After
acquiring sample, running system calibrators, running system
controls, and warming up the instrument, the system resets the
four-hour internal clock.
Accuracy and Precision • Sample uptake volume accuracy ± 5%
• Classification of xMAP microspheres > 80.0%
• Misclassification of xMAP microspheres < 0.5%
• Temperature control 0°C to ± 2°C of target
• Internal sample carry over < 0.9%
• Soluble background fluorescence emission at 575 nm
automatically subtracted from fluorescence intensity value
Sensitivity • Detect 1000 fluorochromes Phycoerythrin (PE) per microsphere
• Reporter channel dynamic range: 3.5 decades