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middle of the microscope field and try to find the ruler clearly. Set the Unit to Pixel
and the video Resolution to the maximum one (640X480 0.35M camera) and Zoom
ratio to 100% (see );
5. Choosing Options>Calibrate••• or clicking “ ” on the toolbar, a red line with
pixels number and 0.000um length is overlaid on the video. At the same time, a
dialog called Calibrate will be displayed over the Video window;
6. Try to align the two ends of the red line with the video ruler scale (Try to pull the
red line as long as possible to keep the calibration accuracy);
7. Enter or select the current microscope objective lens Magnification in the
Magnification field. The current Magnification is 10X;
8. Read the overlaid (by the red line) micro-ruler actual length and fill it in the Actual
Length field, the current Actual Length is 450um. This Actual Length will also be
displayed in the middle of the red line just behind the pixel number. At the same time,