Leica StereoZoom® User Manual
Contents Page Information Safety rules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7 Description: Features, lens system . . . . . . . . . . . . . . . . . . .8 Models . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10 Design System diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12 Incident-light equipment . . . . . .
Dear Customer Thank you for the trust you have shown in choosing one of our products. We hope you will have much enjoyment and success with your new stereomicroscope from Leica Microsystems. With its new StereoZoom® line the Swiss Stereomicroscopy Business Unit offers a comprehensive range of stereomicroscopes for every application. The Leica L2 cold-light illuminator – also new – transforms any of the instruments into a compact, streamlined complete outfit of modern design.
Safety rules General instructions Read the instructions for use and safety instructions before putting the stereomicroscope into operation. Intended application Leica S4 E, S6 E, S6, S6 T, S6 D and S8 APO stereomicroscopes are precision optical instruments for making technical and scientific objects, object details or specimens more easily visible by means of magnification. Stands, illuminators and accessories complete the equipment.
Safety regulations Workplaces with stereomicroscopes facilitate and improve the viewing task, but they also impose high demands on the eyes and holding muscles of the user. Dependent upon the duration of uninterrupted activity, this may lead to asthenic trouble and muscular-skeletal pain so that suitable measures must be taken to reduce the strain: – perfect design of workplace, work content and work process (frequently changing activities).
Mains voltage Connections Changing lamps Make sure that the instrument is set for the correct mains voltage. Incorrect setting can damage the instrument. Only devices with the correct power rating may be connected to the transformers. Overloading can result in damage to the instrument. • • • Statutory regulations 6 Disconnect the mains cable from the mains supply before changing lamps. Never put your hand in the lamp housing or transmittedlight stand when the instrument is connected to the mains.
Symbols User Manual Your instrument is accompanied by a printed English user manual. Additional language versions and information can be found on the interactive CD-ROM. User manuals and updates are available for download on our homepage at www.stereozoom.com. You will find the address of the closest local representative as well as valuable information about products and services from Leica Microsystems on our homepage at www.leica-microsystems.
Description Greenough optical system The optical system of the Leica StereoZoom® line consists of two beam paths converging at 12°. Since the pairs of objectives are very close together, the stereomicroscopes can be constructed very “slim” towards the base. This has a number of advantages: less space required for use on bonders and machines, unimpeded working on the object, plenty of room for tools, free view of the object field.
World first Leica StereoZoom® S8 APO is the first stereomicroscope on the market with completely apochromatic corrected Greenough system. Apochromatic optics corrects perfectly chromatic aberrations, eliminates interfering color fringes and renders even the finest details ultra sharp. Contrast, brilliance, sharpness, resolution, color fidelity and reproduction accuracy are unsurpassed.
StereoZoom® Leica S4 E StereoZoom® Leica S6 E StereoZoom® Leica S6 Leica Design by Christophe Apothéloz 10 Leica StereoZoom User Manual – Description
StereoZoom® Leica S6 T StereoZoom® Leica S6 D StereoZoom® Leica S8 APO Leica StereoZoom User Manual – Description 11
Design 3 Low Eyepoint Eyepieces Eyepieces, fixed Standard 10×/23 16×/16 10 446 354 20×/12 10 446 356 1 StereoZoom® Optics carriers Eyepieces, adjustable* Standard ergonomic 10×/23 10 447 131 16×/16 10 446 355 10 447 133 20×/12 10 446 357 10 447 135 ergonomic 10 447 130 10 447 132 10 447 134 * Adjustable eyepieces accept reticles 2 Objectives 3 Eyepieces 4 Carriers/ Focus arms Leica StereoZoom® System diagram 1 5 Focus columns Leica S4 E 10 446 293 6 Incident-light bases Leica S6 E 10 446 294 Le
High Eyepoint Eyepieces Eyepieces, fixed ergonomic 10×/23 10 447 136 16×/15 10 447 138 Eyepieces, adjustable* ergonomic 10×/23 10 447 137 16×/15 10 447 139 * Adjustable eyepieces accept reticles 10 447 036 Spacing Ring required with eyepieces 10 445 302, 10 445 303 25×/9.5B 10 445 302 40×/6B 10 445 303 Photo / Video documentation Leica DC cameras 2 Leica S6 T 10 446 296 Leica S6 D 10 446 297 Leica S8 APO 10 446 298 Objectives for S8 APO 0.32× 10 446 334 APO 0.63× 10 446 335 APO 1.
Design, Basic equipment The components 1 Incident-light base with stage plate 6 2 Transmitted-light base with glass stage plate 3 Focus column with microscope carrier 4 StereoZoom® optics carrier 4 5 Optional additional objective 6 Eyepieces, fixed and/or adjustable 5 3 1 2 14 Leica StereoZoom User Manual
Assembly Never loosen the 3 screws on the right-hand side of the focus column. Focus column ➔ Incident-light base 왘 Remove stage plate. 왘 Insert 3 hexagon head screws through the underside of the baseplate and screw tight in the focus column. 왘 Replace stage plate. Transmitted-light base ➔ Incident-light base 왘 Remove glass stage plate. 왘 Undo locking device. 왘 Place incident-light stand on the transmitted-light base and engage in the connecting screw. 왘 Close locking device.
Assembly Optics carrier ➔ Stand 왘 Insert optics carrier carefully in the microscope carrier. 왘 Fix optics carrier in the desired position with the clamping screw. Additional objective (optional) ➔ Optics carrier 왘 Screw selected objective tight in counterclockwise direction. Lens shield (optional) 왘 Screw the lens shield tightly into the thread on the StereoZoom® or onto the additional objective. Eyepieces ➔ Eyepiece tube 왘 Push eyepiece into the eyepiece tube as far as it will go.
Soft eyecups The eyepieces are supplied with soft eyecups, which can be attached to achieve the following: – Prevent eye infections if several users are working at the same instrument (see p. 5). – Protect your glasses from being scratched. Leica L2 cold-light source ➔ Stand With its fibre-optic light guides the Leica L2 cold-light source is the illumination of choice for the Leica S4 E, S6 E, S6 and S6 T stereomicroscopes.
Assembly Graticule ➔ Adjustable eyepieces The following graticules and stage micrometers are available for calibration: Graticule 10mm/0.1mm Graticule 5mm/0.1mm Graticule 5mm/0.05mm Graticule 100 div./0.002" Graticule 100 div./0.001" Graticule 150 div./0.0005" Crosshair Stage micrometer 50mm, 0.1/0.01mm divisions Stage micrometer 1", 0.001" divisions For photography, a 10× format reticle is available.
왘 Pick up the graticules at the edge so as not to leave fingerprints and slide into the holder from the side. 왘 Put the insert back into the eyepiece and press firmly in place. 왘 Insert the eyepiece into the eyepiece tube and line up the graticule by turning the eyepiece in the eyepiece tube. The measuring process is described in the “Measuring” instructions.
Assembly 1”, 2/3”, 1/2”, 1/3” C-Mount, CCD cameras Leica DC cameras SLR camera housings Leica MPS30 / MPS60 Photoautomats 1” 3-Chip 2/3” 3-Chip 1/2” C-Mount B-Mount B-Mount CCD camera CCD camera CCD camera 11 543 703 11 543 702 11 543 706 Adapter SLR 10 162 226 10 445 541 10 446 261 2/3", 0,63× 11 541 006 1”, 1× 11 541 007 2/3”, 0,63× 10 445 929 1/2”, 0,5× 10 446 307 2/3", 0,8× 11 541 038 0,55× – 1,1× 10 446 120 8× 10 445 304 10× 10 445 305 16× 10 446 175 10 404 207 10 445 932 10 445 928 1/
Camera assembly for Leica S6 D and S8 APO Leica StereoZoom® S6 D and S8 APO are equipped with a built-in video/photo tube that allows for simple, quick setting up of digital, film and analog video cameras. Please ask your Leica advisor about the options. You can find detailed information about Leica camera systems, accessories and image management software in the corresponding manuals. 왘 Remove the dust cover from the video/photo output.
Operating Overview 6 5 8 1 2 4 7 9 Standard controls and their functions 7 3 1 Magnification changer, right-hand pinion knob with magnification scale 2 S6 models: zoom limiter stop 3 Focusing drive 4 Fixing screw fixes the optics carrier in the microscope carrier 5 Adjustable eyepiece tubes: Interpupillary distance adjustable from 55–75mm 6 Eyepieces and eyecups 7 Lamp bracket mounting thread (on both sides and at rear) 8 Socket for connecting for the Leica L2 universal light guide (not for Leica S8 A
Preconditions for working comfortably • Pay great attention when setting up your stereomicroscope. You will only benefit fully from the outstanding optical and ergonomic advantages of the instrument if you set it up precisely in accordance with the directions already given. • Arrange your workplace as well as possible, and pay attention to the heights of chair and table. • When sitting, use the entire seat area and also the back support of the chair. • Support your forearms.
Operating Eyecups The eyepieces are supplied with detachable eyecups (see also p. 5). If you do not wear spectacles and want the eyecups to fit snugly round your eyes: 왘 Attach the eyecups to the eyepieces. You wear spectacles for your work. 왘 Fold the eyecups downwards. • The folded-down eyecups will protect your spectacle lenses against scratching.
Tension adjustment Is the focusing drive too stiff/too loose or does the set-up slip down unassisted? You can tension the focusing drive to suit the weight of the set-up and your own needs: 왘 Take one pinion knob in each hand and turn in opposing directions until the desired tension is reached. Tilting the optics carrier to a lateral position The optics carrier can be tilted sideways if the user wants to work from a lateral position: 왘 Loosen the clamping bolt.
Operating Setting the zoom limiter On the S6 models and for the S8 APO it is possible to fix the maximum and minimum zoom. The same function can be used to set a fixed level of magnification. Example: Setting a zoom range of 1 to 3.2 1. Set the minimum zoom level at 1 using the stop on the left-hand pinion knob: 왘 Loosen the hexagon head screws on the left-hand pinion knob using the Allen key supplied. 왘 Turn the right-hand pinion knob to 1.
2. Set the maximum zoom level at 3.2 using the stop on the right-hand pinion knob: 왘 Loosen the hexagon head screws on the right-hand pinion knob. 왘 Turn the right-hand pinion knob to 3.2. 3.2 왘 Move the stop on the righthand pinion knob backwards until it touches the built-in zoom stop. 왘 Tighten the hexagon head screws.
Operating Diopter adjustment and setting parfocality If you adjust the diopters at the adjustable eyepiece as described below, the focus will remain constant, or parfocal, from minimum to maximum magnification. In other words, you will not have to refocus when you change magnification, but only when you want to examine a higher- or lower-positioned object segment. Make full use of this feature, which is not available on every stereo microscope. • Diopter adjustment is possible within a range from +5 to –5.
Diopter adjustment at the adjustable eyepiece 왘 Without looking into the eyepieces, turn the eyelens of the adjustable eyepiece as far as possible in the “+” direction. 왘 Close the eye that is looking into the fixed eyepiece and look into the adjustable eyepiece with the other eye. 왘 View the test object and slowly turn the eyelens clockwise (the “–” direction) until the object is in focus. 왘 Set the microscope to maximum magnification.
Special notes Setting up diopter with two adjustable eyepieces Preparations 왘 For Leica S6 D and S8 APO, set pinion knob to Vis position. 왘 Set approximate working distance by means of focusing drive (working distances of various objectives see p.38–40). 왘 Set up lighting 왘 Set viewing distance (p.23). 왘 Set ‘0’ diopters on both eyepieces. Focusing on test object 왘 Place flat test object under the objective. 왘 Set lowest magnification.
Regulating diopters 왘 Set lowest magnification. 왘 Do not look into the eyepiece! 왘 Turn lens counterclockwise in the ‘+’ direction until it stops. 왘 Now look into the eyepiece. 왘 Close one eye. 왘 With the other eye, observe the test object and turn the lens slowly clockwise in the ‘–’ direction, until this eye sees the object clearly. 왘 Set the diopter for the other eye in the same way. 왘 Select highest magnification. 왘 View object and, if necessary, gently refocus.
Operating Photography with Leica S6 D and S8 APO It is possible to switch between the observation and photo beam paths. Light distribution: – in ‘Vis’ position: 100% light in both eyepieces/no light in the video/photo beam path – in ‘Doc’ position: 100% light in the right eyepiece/no light in the left eyepiece/100% light in the video/photo beam path DOC VIS Focusing and framing down the left eyepiece (video/photo beam path).
Special notes What do I do if … Remedy … the field of vision is in shadow? • Adjust interpupillary distance and ensure that it is correct (p.23). • Check pupil position (p.23). … the image will not stay in focus? Remedy • Insert eyepieces correctly (p.16). • Correct diopter adjustment exactly as described (p.28–31). … the focusing drive slides down or is difficult to turn? Remedy • Adjust tension (p.24).
Care In this section we would like to explain how you can take care of your microscope and give you a few tips on cleaning. Protect your microscope • against moisture, vapours, acids, alkalis and caustic substances. Never keep chemicals close to your microscope. • against improper handling. Never fit other equipment connectors or dismantle optical systems and mechanical parts unless the user manual gives express instructions for doing so. • against oil and grease.
Cleaning plastic parts Various components are made of plastic or are plasticcoated to make them pleasant to touch and handle. However, incorrect cleaning with unsuitable detergents can damage the plastic. Please observe the following do’s and don’ts: Never clean plastics • in an ultrasonic cleaner. The plastic may become brittle and eventually break. • with caustic or acetone-containing substances such as ether substitute. • with any other solvents except ethanol and isopropanol.
Special notes We guarantee quality You are working with a high-performance precision instrument, whose quality we guarantee. The warranty covers manufacturing and materials faults, but not damage resulting from negligence or incorrect handling. If you treat your valuable optical instrument with due care, it will repay you with the decades of reliable precision for which Leica instruments are renowned.
Calculating total magnification and field diameter MO ME z NFOV Objective magnification Eyepiece magnification Magnification changer setting Eyepiece field number. Field numbers are printed on the eyepieces: 10×/23, 16×/16, 20×/12, 10×/23B, 16×/14B, 25×/9.5B, 40×/6B Example: MO 1.6× objective ME 20×/12 eyepiece z Zoom position 4.0 Magnification in the binocular tube: MTOT VIS = MO × ME × z 1.6 × 20 × 4 = 128 Field diameter in the object: NFOV ∅OF = __________ MO × z 12 __________ 1.
Optical Data, Leica S4 E, S6 E, S6, S6 T, S6 D with additional objectives 110mm 40×/6B 10 445 303 38 Field diameter mm 10 445 302 Total magnification 25×/9.5B Field diameter mm 10 445 301 Total magnification 16×/14B Field diameter mm 10 446 356 10 446 357 Total magnification 20×/12 Field diameter mm 10 446 354 10 446 355 1.6× 55mm Total magnification 16×/16 0.75× 130mm Field diameter mm 10 446 326 10 446 329 0.63× 155mm Total magnification 10×/23 10×/23B 0.
with additional objectives Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm 98mm Total magnification 0.7×–1.0× 48mm Field diameter mm 0.7×–1.0× 137mm Total magnification 0.6×– 0.75× 77mm Field diameter mm 0.6×– 0.75× 350mm Total magnification 0.3×– 0.4× 200mm Field diameter mm 0.3×–0.4× 35mm Total magnification 2.0× 12.6 16.0 20.0 25.0 32.0 40.0 50.0 64.0 80.0 18.3 14.4 11.5 9.2 7.2 5.8 4.6 3.6 2.
Optical Data, StereoZoom® S8 APO Apochromats 75mm 10 445 303 40 1.0 1.25 1.6 2.0 2.5 3.2 4.0 5.0 6.3 8.0 10.0 12.5 16.0 20.0 25.0 32.0 40.0 50.0 63.0 80.0 23.0 18.4 14.4 11.5 9.2 7.2 5.8 4.6 3.7 2.9 6.3 7.9 10.1 12.6 15.8 20.2 25.2 31.5 39.7 50.4 36.5 29.1 22.8 18.3 14.6 11.4 9.1 7.3 5.8 4.6 16.0 20.0 25.6 32.0 40.0 51.2 64.0 80.0 100.8 128.0 14.4 11.5 9.0 7.2 5.8 4.5 3.6 2.9 2.3 1.8 20.0 25.0 32.0 40.0 50.0 64.0 80.0 100.0 126.0 160.0 11.5 9.2 7.2 5.8 4.6 3.6 2.9 2.3 1.8 1.4 3.2 4.0 5.1 6.4 8.
Dimensions, Leica S4 E, S6 E, S6 T with incident-light stand 38° 161 46 Dimensions in mm 134 ø76 23 107 22 110 ø58 223 81 351 25 Leica S4 E, S6 E, S6 T 278 393 144 104 113 148 278 393 205 205 Leica StereoZoom User Manual 41
Dimensions, Leica S4 E, S6 E, S6 T with transmitted-light stand 38° 161 46 Dimensions in mm 134 ø76 23 65 22 110 ø58 107 278 393 144 104 113 148 205 278 393 220 205 42 Leica StereoZoom User Manual 223 81 351 25 Leica S4 E, S6 E, S6 T
Dimensions, Leica S6 Dimensions, Leica S6 D with inclining focusing drive with incident-light stand 46 Leica S6 D 207 38 60° 120 134 ø76 223 351 110 ø15.8 ø58 25 25 ø76 35 107 148 23 26 22 278 393 144 104 Dimensions in mm 144 104 Dimensions in mm 150.
Dimensions, Leica S6 D with incident-light stand 171 Leica S6 D 223 Dimensions in mm 351 81 25 38 207 134 ø76 23 65 22 110 ø58 107 278 393 144 104 113 148 205 278 393 220 205 44 Leica StereoZoom User Manual
Dimensions, Leica S8 APO with incident-light stand 38 207 23 107 223 134 ø58 ø76 22 75 116 351 25 171 Leica S8 APO 278 393 Dimensions in mm 144 104 113 148 278 393 205 205 Leica StereoZoom User Manual 45
Dimensions, Leica S8 APO with transmitted-light stand 38 207 23 223 134 ø58 ø76 65 22 75 116 351 25 171 Leica S8 APO 107 Dimensions in mm 278 393 144 104 113 148 205 278 393 220 205 46 Leica StereoZoom User Manual
Leica Microsystems worldwide Leica Microsystems is active in microscopy, sample preparation, image analysis, confocal laser technology, medical equipment and equipment for the semiconductor industry. With headquarters in Wetzlar, Germany, this international technology group has grown from five companies with a long tradition – Wild, Leitz, Reichert, Jung and Cambridge Instruments.
Leica Microsystems – the brand for outstanding products Leica, the leading brand for microscopes and scientific instruments, developed from five brand names, all with a long tradition: Wild, Leitz, Reichert, Jung and Cambridge Instruments. Yet Leica symbolizes innovation as well as tradition.
