Leica M125 Leica M165 C Leica M205 C Leica M205 A Manual
General Instructions Safety concept Before using your microscope for the first time, please read the "Safety concept" brochure included with your instrument. It contains additional information about handling and care. Use in clean rooms The Leica M series can be used in clean rooms without any problems. Cleaning ★★ Do not use any unsuitable cleaning agents, chemicals or techniques for cleaning. ★★ Never use chemicals to clean colored surfaces or accessories with rubberized parts.
Safety concept The individual modules of the Leica M stereomicroscopy series include an interactive CD-ROM with all relevant user manuals in 20 other languages. Keep it in a safe place, and readily accessible to the user. User manuals and updates are also available for you to download and print from our web site at www.stereomicroscopy.com.
Symbols used Warning of a danger This symbol indicates especially important information that must be read and complied with. Failure to comply can cause the following: • ★★ Hazards to personnel ★★ Functional disturbances or damaged instruments Warning of hazardous electrical voltage This symbol indicates especially important information that, if not observed, can cause the following: ★★ Hazards to personnel ★★ Functional disturbances or damaged instruments.
Safety regulations Description The individual modules fulfill the highest requirements for observation and documentation of Leica stereo microscopes of the M series. non-Leica components that are outside of the scope of this manual.
Safety regulations (continued) Repair, service work ★★ See “Safety concept” booklet ★★ Only original Leica Microsystems spare parts may be used. Installation in third-party products ★★ See “Safety concept” booklet Disposal ★★ See “Safety concept” booklet ★★ Before opening the instruments, switch off the power and unplug the power cable. ★★ Touching the live circuit can cause injury.
Safety regulations (continued) Direct contact with eyepieces can be a potential transmission path for bacterial and viral infections of the eye. The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups. Light sources: safety instructions Protective measures of the manufacturer: ★★ UV protection screen in front of the specimen plane prevents the user from looking directly into the UV rays.
Safety regulations (continued) Supply unit Always unplug the supply unit from the power supply: ★★ When installing and disassembling the lamp housing ★★ Before opening the lamp housing ★★ When replacing the high-pressure mercury lamp and other parts, such as the heat-absorbing filter or the collector Lamp housing ★★ Never open the lamp housing while the lamp is switched on. Risk of explosion, UV exposure, blinding! ★★ Before opening the lamp housing, allow it to cool off for at least 15 minutes.
Table of Contents General Instructions Safety concept Symbols used Safety regulations 2 3 4 5 The Leica M Series Congratulations! A Step Towards Infinity The Electronics: Comfort, Convenience and Safety The Modular Design: Everything is Relative Maximum Compatibility On We Go 13 14 15 16 17 18 Assembly Base and Focusing Column Optics carrier Tube Eyepieces Objective Objective Nosepiece – Assembly Objective Nosepiece – Adjusting Parfocality AX Carrier – Preparation AX Carrier – Assembly
Eyepieces Magnification Factors of the Eyepieces Health Notes Dioptric correction Dioptric Correction and Parfocality Graticules 58 59 60 61 62 Photography & Video Photography & Video Photo tubes and C-mounts Trinocular video/phototube 50% Trinocular video/phototube 100% 64 65 66 67 Microscope Carrier The Microscope Carrier AX The Objective Nosepiece 69 71 Objectives andoptical accessories The Different Types of Objectives Leica M series 73 Bases Transmitted-light Base TL ST: Controls
Accessories Leica PSC Controller 95 Dimensional Drawings Leica M125 / M165 C Leica M205 C Leica LED5000 MCI Leica LED5000 RL 97 101 104 105 Specifications for the Bases Transmitted-light Base TL ST Transmitted-light Base TL BFDF TL RC™ / TL RCI™ IsoPro™ Motorized XY Stage 107 108 109 110 Appendix Calculating the Total Magnification and Field of View Diameter Care, Maintenance, Contact Persons 112 113 Leica M series Manual 11
The Leica M Series Leica M series Manual 12
Congratulations! Congratulations on obtaining your new Leica M series stereomicroscope. We are convinced it will exceed your expectations, as never before have we applied our decades of experience in the areas of optics, mechanical engineering and ergonomics in such an uncompromising manner. The M series embodies all the qualities you associate with the name Leica Microsystems: excellent objectives, high-quality engineering, and reliability.
A Step Towards Infinity Ever since their introduction by Horatio S. Greenough, stereomicroscopes have worked according to the optical principles based primarily on Ernst Abbe's research. For over a century, ingenious optics designers and engineers have worked to push magnification, resolution and image fidelity to the limit permitted by optics.
The Electronics: Comfort, Convenience and Safety for your Experiments Never before have electronics been used as extensively in a Leica series as in the new M series. Optics carrier, tripod, base and illuminator are all connected using electrical contacts—which provides a number of advantages. Reliability for your experiments Exact reproducibility of results is an increasingly common demand, particularly in research and development.
The Modular Design: Everything is Relative The Leica M series provides maximum flexibility in choosing equipment, thanks primarily to the modular configuration and the compatibility that Leica has painstakingly maintained for decades. The optics carriers, eyepieces, bases and more can be combined in any way you choose, allowing you to create the microscope that best suits your needs. Leica M series Despite this, you will notice that the controls and individual components do not differ significantly.
Maximum Compatibility Leica engineers were careful to ensure that the new M series—like its predecessors—remains compatible with existing series. This means that objectives, bases, tubes and so on can be reused. Objectives All new objectives of the M series are parfocal, meaning that when used with the objective nosepiece, they can be replaced while keeping the specimen in perfect focus. If you prefer, you can continue to use the previous objective series. In this case, parfocality is no longer guaranteed.
On We Go If your new Leica microscope has already been assembled and commissioned by your Leica consultant, click here to skip through the installation instructions and go directly to the Quick Start Guide on page 46. Leica M series If, on the other hand, you are assembling the microscope yourself, continue with the "Assembly" chapter, which begins on page 19.
Assembly Leica M series Manual 19
Base and Focusing Column The first step is to connect the focusing column of the M series to the corresponding base. Assembling the column adapter 1. Securely install the column adapter on the column using the four included screws. Assembling the focusing drive 2. Securely screw the focusing column to the base using the six included screws.
Optics carrier Tools used ★★ Hex socket screwdriver, 4 mm Leica M series Assembling the optics carrier 1. Place the optics carrier on the focusing column so that the screw fit into the thread provided and the lug fits into the groove. Manual 2. Press the optics carrier backwards to the focusing column and screw it in place using your other hand.
Tube All intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner. Tools used ★★ No tools required. Assembling the tube 2. Push the tube (for example, the inclined binocular tube) into the dovetail ring and rotate it slightly in both directions until the positioning screw meshes with the guide groove. 3. While holding the tube only slightly, carefully tighten the positioning screw. It is automatically brought to the correct position. Preparations 1.
Eyepieces Tools used ★★ No tools required. Magnification range You can extend the overall magnification range using available 10×, 16×, 25× and 40× widefield eyepieces for persons wearing glasses. Preparation 1. If you want to use an optional graticule, insert it now (page 62). Inserting the eyepieces 3. Push the eyepieces into the tubes as far as they will go and check to ensure that they fit tightly and accurately. 2. Remove the plastic tube guard. 4. Securely tighten the clamping screws.
Objective Tools used ★★ No tools required. Preparation 1. Remove the protective cap on the optics carrier by turning it. Attaching the objective Hold the objective firmly during assembly and disassembly so that it does not fall onto the stage plate. This applies particularly to the 2× planapochromatic objective, which is very heavy. Remove all specimens from the stage plate first. • Alternative fastening options ★★ If using the objective nosepiece, read the instructions on page 25.
Objective Nosepiece – Assembly Preparations Hold the objectives firmly during assembly and disassembly so that they do not fall onto the stage plate. • 2. Rotate the moving part by 90° and attach the objective nosepiece to the drive housing from the front (!). Screw the objective nosepiece firmly into place. ★★ Move the drive housing all the way upwards and remove the optics carrier, if the carrier has already been installed. Assembly 1. Remove the transport anchor from the objective nosepiece. 4.
Objective Nosepiece – Adjusting Parfocality The following procedure only has to be carried out once. Afterwards, both objectives are parfocal, meaning that the specimen remains in focus when the objective nosepiece is rotated. Adjustment 1. Rotate the 2× objective into the beam path and set it to the lowest magnification. 2. Focus on the specimen. This procedure must be repeated if you replace either of the two objectives with another.
AX Carrier – Preparation Tools ★★ Hex socket screwdriver, 2.5 mm ★★ If the switch position corresponds to the optics carrier used, skip the following steps. Otherwise, proceed as follows: Preparing the AX carrier Before the AX carrier is installed, it may first have to be prepared for the optics carrier to be used (Leica M125, M205 C or M165 C). 2. Unscrew the two screws that hold the switch in position. 4. Important: push the switch all the way into the mount while reinserting the screws.
AX Carrier – Assembly Tools ★★ Hex socket screwdriver, 4 mm ★★ Philips screwdriver 2. Install the AX carrier on the focusing column. 4. Unscrew the three holding screws and remove the adapter ring on the optics carrier. Once the AX carrier has prepared for the corresponding optics carrier, it can be installed. 1. Move the slide of the AX carrier into mid position. 3. Screw the objective to the AX carrier. 5. Install the optics carrier on the AX carrier.
Transmitted-light Base TL ST Unpacking the base The base is delivered with the adapter plate installed. Make sure the devices are unpacked on a flat, sufficiently dimensioned, and non-slip surface. Leica M series Focusing drive and column 1. Unscrew the extension plate from the base using the Allen key provided. 2. Attach your focusing drive column to the bottom using the 4 Allen screws. 3. Reattach the adapter plate to its original position using the 6 Allen screws.
Transmitted-light Base TL BFDF: Before First Use Removing the transport anchors Before you can use the transmittedlight base for the first time, it is absolutely necessary to remove the two transport anchors as follows.
Transmitted-light Base TL BFDF Standard delivery The base is delivered with the adapter plate installed. The selected stage (IsoPro™ crossstage or standard stage 10 447 269), and the focusing drive will have to be mounted later. Stage assembly The TL BFDF transmitted-light base can be equipped with three different stages. The selected stage is mounted on the base before commissioning. You can switch between the stages at any time with just a few hand movements. 2.
TL RC™ / TL RCI™ The base is delivered with the adapter plate installed. The selected stage (IsoPro™ crossstage or standard stage 10 447 269), and the focusing drive will have to be mounted later. Standard stage 1. Take the glass plate from the rectangular gap in the standard stage. 2. Ensure that the instruments are unpacked on a level, adequately sized, and nonskid underlay. Stage assembly The transmitted-light base TL RC™/ RCI™ can be equipped with three different stages.
IsoPro™ Manual Cross-stage: Assembly IsoPro™ Cross-stage Before the IsoPro™ cross-stage is mounted to the base, the axis containing the control buttons is attached either on the left or the right side of the cross-stage. Before the IsoPro™ cross-stage is mounted to the base, the axis containing the control buttons is attached either on the left or the right side of the cross-stage.
IsoPro™ Manual Cross-stage: Assembly (continued) 1. Take the glass plate from the cross-stage and turn it around. 2. Change the gear rod from the left to the right-hand side. Control assembly 1. Take the glass plate from the cross-stage and turn it around. 2. Attach the axis with the control buttons to the desired side. The fastener snaps into the cross-stage magnetically. Cross-stage assembly 1. Place the cross-stage on the base. 2.
IsoPro™ Manual Cross-stage: Assembly (continued) 3. Screw the cross-stage evenly onto the three threaded holes. 4. Push the cross-stage all the way back towards the column. 5. Reinsert the glass plate back into the standard stage. Leica M series Focusing drive and column 1. Unscrew the extension plate from the base using the Allen key provided. 2. Attach your focusing drive column to the bottom using the three Allen screws. 3.
IsoPro™ Motorized Cross-stage: Assembly Basics The transmitted light bases of the TL series (TL BFDF, TL RC™, TL RCI™) are supplied with an installed extension plate. The selected stage (IsoPro™ cross-stage or standard stage 10 447 269), and the focusing drive will have to be mounted later. 1. Unpack the cross-stage from the transport packaging and position it on the transmitted-light base. 1 The motorized cross-stage is a sensitive precision instrument.
IsoPro™ Motorized Cross-stage: Assembly (continued) 3. Remove the two M3 screws and the holder from the cross-stage. 4. Remove the two M4 screws and the sleeve from the cross-stage. 5. Remove the four shock-absorbing cartons from the cross-stage. After removal, keep all the transport anchors in the plastic bag for future transport.
IsoPro™ Motorized Cross-stage: Assembly (continued) The bases The TL BFDF, TL RC™ and RCI™ transmitted light bases can be equipped with three different stages: standard stage, manual and IsoPro™ automated cross-stage. The selected stage is mounted on the base before commissioning. You can switch between the stages at any time with just a few hand movements. The following paragraph assumes use of the base without the stage mounted. Disassembly is performed in reverse order of the following steps.
IsoPro™ Motorized Cross-stage: Assembly (continued) Cross-stage for X-Y Stage Control Module 1. Plug the CTL2 plug of the cross-stage motor into one of the three available CTL2 interfaces. As soon as all instruments have been plugged into the control box and it has been plugged into the power supply, the cross-stage initializes and automatically drives to the mid position. 2. Plug the CTL2 plug of the Leica PSC controller into another CTL2 interface. 4.
Cables: Terminals The new Leica M-series features extensive encoding with which various microscope data and settings can be read out, transferred to the PC and reproduced later. ★★ The interface to the optics carrier is on the column. The Terminals The connection to the PC and to other instruments is made using the terminals on the rear side of the column: 1 3 2 1. 2×CTL2 terminals for auxiliary equipment such as the ring illuminator, the base and other accessories from the Leica product range. 2.
Cables: Cable Duct The integrated cable duct in the column enables a neat cable layout around the microscope. For example, the USB or FireWire cables of the camera can be stowed in the cable duct. Feeding the cables 1. Unscrew the three screws on the cable duct. 2. Remove the cover of the cable duct. Leica M series Manual 3. Place the cables in the cable duct and screw the cover on tightly. Tip: Estimate the length of the cable ends you will need before screwing on the cover.
Cables: Diagram Alternative installation Please use the Leica Application Suite to configure the functions of the system. LED5000 RL LED5000 MCI™ 115/230 V 115/230 V DCI Controlbox Power/USB 115/230 V Footswitch 2... Footswitch 1 (Connect to free CTL2-port on last footswitch.
Leica LED5000 MCI™ The Leica LED5000 MCI™ (for "Multi Contrast Illumination") is installed using two screws. For optimum accessibility, the optics carrier should be removed during installation (see page 15.) Assembly 1. Hold the LED5000 MCI™ with one hand and tightly screw the retraining stirrups on both top holes on the drive housing. • 2. Connect the CAN-bus cable to either of the two sockets. (The flat part of the plug must be facing downwards.) 3.
Leica LED5000 MCI™: Alternative Assembly Under certain circumstances, the light source must not be moved along with the optics carrier. A typical example is multifocus images in which the Z-stack changes while the angle of incidence of the light must remain the same. For such purposes, the Leica LED5000 MCI™ is directly fastened to the column. 2. Screw the retaining stirrup into the column at the notch using the single screw.
Leica LED5000 RL The ring illuminator, the Leica LED5000 RL ("Ring Light"), is installed on the objective using a single screw. It has been optimized for a working distance between 60 and 70 mm. Constraints The Leica LED5000 RL can be used in conjunction with the planapochromat 1× and planapochromat 0.63× objectives. With all other objectives, the working distance is too low for adequate illumination. Assembly 1. Connect the CAN-bus cable to the ring illuminator.
Quick Start Guide Leica M series Manual 46
The Fastest Route to Success Your Leica stereomicroscope has been delivered in completely assembled condition by your Leica partner, and naturally you want to get right to work. Therefore, your next step should be to study the Quick Start Guide, which outlines the most important steps at a glance. This manual will then familiarize you with the finer details of your microscope. The following pages contain important, practical information that makes using it every day easier.
Overview of an M series Microscope Click an entry or a position on the microscope to go directly to the corresponding part of the manual.
The Correct Interpupillary Distance The interpupillary distance is correctly set if you see a single circular image field when looking at a specimen. If you are still a novice microscope user, you may need a short time to become accustomed to this. Not to worry—after a little while, it will become automatic. Adjusting the Interpupillary Distance 1. Look into the eyepieces. 3. Slowly approach the eyepieces with your eyes until you can see the complete image field without corner cutting. 2.
Using the Eyepieces The eyepieces form the connection between the tube and the eye of the observer. Simply push them into the tube and they are ready to use. If you do not wear glasses: 1. Hold the eyepiece firmly and rotate the eyecups forwards counterclockwise. 2. If an eyepiece is equipped with the inte- If you wear glasses: 1. Hold the eyepiece firmly and rotate the eyecups counterclockwise towards the rear, as otherwise the viewing distance is too great.
Focusing Focusing raises or lowers the stereomicroscope using the focusing drive. The specimen detail is brought into sharp focus as soon as it is in the focal point of the objective. ★★ The focusing drive can be operated either left- or right-handed. Focusing ★★ The inner, coarse adjustment is used for covering great distances. Coarse/fine adjustment The sharpness is adjusted using the coarse/fine adjustment. ★★ The outer, fine adjustment is used for fine focusing. • load of up to 15 kg.
Adjusting the resistance of the focus drive Adjusting the resistance Is the focus movement too loose or too tight? Does the outfit tend to slide downwards? The resistance can be adjusted individually depending on the equipment weight and personal preferences as follows: 1. Grip the outer drive knobs with both hands and turn them towards each other until the desired resistance is reached during focusing.
Changing the magnification (zoom) All M series microscopes have an integrated zoom. The name indicates the zoom range covered: Zooming 1. Look into the eyepieces. 2. Focus on the specimen. ★★ Leica M125 = 16.5:1 ★★ Leica M165 C = 16.5:1 ★★ Leica M205 C = 20.5:1 3. Rotate the magnification changer until the desired magnification is configured. The rotary knob for the zoom can be used either left or right-handed.
Ratchet Steps and Magnification Levels The zoom button can optionally be operated either with or without ratchet steps. Continuous zoom is possible when the ratchet steps are disabled, which many users find convenient. On the other hand, when the ratchet steps are enabled, photographs, measurement results etc. can be reproduced more accurately. Leica M series Enabling and disabling ratchet steps 1. Push the top button downwards to disable the ratchet steps. 2.
Parfocality: More Comfort and Convenience for Your Work All Leica stereomicroscopes are parfocally matched, meaning that you can view a focused specimen from the lowest to the highest magnification without having to refocus. There is no need to readjust the focus to view of specimen location that is located higher or lower. Requirements for parfocal work ★★ If you are using an eyepiece with dioptric correction, the procedure differs from this description. For more information, refer to page 61.
Iris Diaphragm The iris diaphragm in the optics carrier of your M series microscope has the same purpose as those in a camera shutter: it regulates the available light, which changes the depth of field. The "depth of field" is the area of a specimen that is brought into sharp focus. Leica M series Closing the iris diaphragm ★★ Close the iris diaphragm by turning the knob to the left. The subject appears darker and the depth of field increases.
Eyepieces Leica M series Manual 57
Magnification Factors of the Eyepieces An eyepiece not only makes it possible to look passively into the microscope, but also has a critical effect on the maximum magnification. The magnification factor is between 10x and 40x.
Health Notes Potential sources of infection Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the eye. The risk can be kept to a minimum by using individual eyepieces or detachable eyecups. Eyecups can be ordered separately. Please contact your Leica partner. • Separate eyecups are an effective way of preventing infections.
Dioptric correction All Leica eyepieces are also available with built-in dioptric correction, allowing the microscope to be used without glasses even by those with vision problems. The correction comprises ±5 diopter settings. Using the Dioptric Correction 1. Set the dioptric correction of both eyepieces to the mid position ("0" diopter settings. 2. While wearing your glasses, look through the eyepieces and focus on the specimen.
Dioptric Correction and Parfocality Leica stereomicroscopes are parfocally matched. The prerequisite for this is the correct setting of the diopters and the parfocality. The following adjustments only have to be carried out once by each user. Adjusting 1. Set the dioptric correction for both eyepieces to "0". Preparations ★★ Move the lever of the video/phototube to the "observation" position and open the diaphragm. 3. Select the highest magnification and readjust the sharpness.
Graticules Use Graticules make length measurements and counting easier, particularly for workstations that are not equipped with a digital camera and LAS software. 2. Clamp the graticule on the insert, applying moderate pressure. Ensure that the graticule fits tightly. 4. You can now align the graticule by rotating the eyepiece in the tube and then tightening it using the clamping screw. The graticules for length measurements and numbering are fitted in mounts and are inserted into the eyepieces. 1.
Photography & Video Leica M series Manual 63
Photography & Video For most microscope users, digital documentation has become an invaluable part of their work. Research results can be presented in an attractive manner; measurements on the digital image provide clarity and, in conjunction with the motorized IsoPro™ cross-stage, even images of large specimens can be captured step by step and automatically joined to create a new complete image.
Photo tubes and C-mounts Application All Leica DFC cameras are equipped with a standardized C-mount interface. In turn, the C-mount adapter for the respective trinocular tube is connected to this interface. This adapter creates a solid mechanical connection between the microscope and camera and ensures optimum rendering of the microscopic image on the image sensor of the camera.
Trinocular video/phototube 50% Use With its third beam path, the trinocular video/ phototube 50% enables you to simultaneously view and photograph a specimen. The available light is divided as follows: ★★ 50% is available for the two eyepieces. ★★ 50% of the light is diverted to the video/ photo beam path. Assembly Fasten the "trinocular tube 50%" to the optics carrier instead of the binocular observation tube (refer to page 22).
Trinocular video/phototube 100% Use With its third beam path, the trinocular video/ phototube 100% enables you to either view or photograph a specimen. This means that 100% of the light is available to one or the other beam path. The other beam path remains opaque or black. Assembly Fasten the "trinocular tube 100%" to the optics carrier instead of the binocular observation tube (refer to page 22).
Microscope Carrier Leica M series Manual 68
The Microscope Carrier AX Users who intend to do a great deal of photography, take measurements or work with polarization should select the microscope carrier AX for stereoscopic/axial observation. The parallaxfree imaging by means of a perpendicular beam path leads to results without parallax error. Stereoscopic viewing A stereoscopic view is required for examining spatial objects. For this purpose, the microscope carrier AX must be brought to its home position. 1. Unscrew the clamping screw.
The Microscope Carrier AX (continued) Parallax-free images The parallax-free imaging produces more precise results in photography, measuring and polarization. 1. Unscrew the clamping screw. 2. Push the microscope towards the left as far as it will go and tighten the clamping screw. Leica M series The objective is now located directly beneath either the left or the right beam path.
The Objective Nosepiece Use The objective nosepiece enables you to switch between two objectives using just one hand movement, for example between a 1× and a 2× planapochromat. Parfocal work With the new M series objectives, the parfocality is maintained even when objectives are changed, meaning that the specimen remains in sharp focus during the change.
Objectives and optical accessories Leica M series Manual 72
The Different Types of Objectives To meet the various requirements regarding imaging properties, there is a choice of highquality interchangeable planachromatic and planapochromatic objectives and also lowerpriced interchangeable achromatic objectives. ★★ Achromatic objectives are particularly suited for specimens with high-contrast structures. ★★ Flat-field (planachromatic) objectives are particularly well suited for studying flat objects such as wafers and thin sections. Achromatic objectives The 0.
Bases Leica M series Manual 74
Transmitted-light Base TL ST: Controls 1 2 1 3 4 1 2 3 4 3 Extension plate of the Transmitted-light Base TL ST Rear side of the transmitted-light base TL ST 1 Screws for changing the halogen lamp 2 Power connection socket 3 Power switch Adapter plate for easy assembly of focusing drives Removable glass plate Controller for light intensity Adjustment for deflection mirror Leica M series 2 Manual 75
Transmitted-light Base TL ST: Operation Light intensity control The left control adjusts the intensity of the 12 V/20 W halogen illumination. 1. Switch on the illumination of the base at the power switch. Transmitted-light control The transmitted-light base TL BFDF has a slider that automatically moves the deflection mirror in the base when moved. The mirror is kept in the correct position at all times and permits smooth changeover between bright field and opaque transmitted light. 3.
Transmitted-light Base TL ST: Lamp Replacement Changing the Halogen Lamp Before you change the lamp, it is absolutely necessary to unplug the power plug from the base to prevent the risk of electric shock! • Changing bulbs 1. Unscrew the two screws on the cooling unit and pull the cooling unit out, along with the lamp. Precautionary measures When inserting the lamp, ensure that the cables are inside the two metal clamps. This prevents the cables from getting caught during insertion.
Transmitted-light Base TL BFDF: Controls 1 2 Adapter at the focusing drive 3 1 2 3 Extension plate of the Transmitted-light Base TL BFDF Adapter plate for easy assembly of focusing drives Standard stage 10 447 269 Button to toggle between bright field and dark field Button to toggle between bright field/ dark field Leica M series Manual Connector for cold light sources (light conductor active f = 10 mm, end tube f = 13 mm) 78
Transmitted-light Base TL BFDF: Operation Light intensity control Please observe the user manual—in particular, all safety regulations—from the manufacturers of the light guide and cold light source. • ★★ Switch on the cold light source according to the manufacturer's user manual and adjust the brightness. Bright field Bright field is suitable for examining translucent objects featuring contrasting structures.
TL RC™ / TL RCI™: Controls 1 2 1 2 3 4 5 6 7 3 45 Vertical column - transmitted-light base adapter plate 1 2 3 4 5 6 7 Heat sink of the integrated halogen illumination (only TL RCI™) Extension plate for focusing drives Standard stage 10 447 269 Filter holder Control of top and bottom flaps of the Rottermann Contrast™ Button for mirror and horizontal movement of the mirror Transmitted-light base Leica M series Manual 1 2 3 4 5 6 6 Power switch Power connection socket USB socket, type B USB socket
TL RCI™: The Deflection Mirror 1 Inverted world? Depending on the properties of the specimen (refractive index of the environment) and the perception of the viewer, it is possible that the switches described below for positive and inverted relief contrast are to be operated in reverse. This means that the lower switch, rather than of the upper switch, controls inverted relief contrast and vice-versa.
TL RCI™: Color Intensity and Temperature The transmitted-light base TL RCI™ has two electronic potentiometers that control the color intensity (1) and color temperature (2). The controller for the color temperature simultaneously serves as an electronic shutter: ★★ To interrupt your work, click the potentio meter (2).
TL RC™ / TL RCI™: Operation Setting the relief contrast The two switches on the left side of the transmitted-light base TL RC™/TL RCI™ activate two built-in flaps. The outer switch (1) controls the inverted relief contrast, and the inner switch (2) adjusts the positive relief contrast. 1 1 2 3 2 Depending on the flap position, a part of the opening of the built-in Fresnel lenses are covered, which results in the different contrast effects.
TL RCI™: Methods in Transmitted Light Vertical bright-field illumination Suitable for stained amplitude specimens with sufficient contrast. The light beams are deflected vertically through the specimen. This results in an accurate bright field with maximum brightness. Leica M series Inclined transmitted light Suitable for semitransparent, opaque specimens such as foraminifera and fish eggs. Move the deflection mirror until the desired data are visible.
TL RCI™: Relief Images Starting position 1. Push the deflection mirror all the way back towards the column. 2. Turn the deflection mirror into the notch position at an angle of 45°. Leica M series Positive relief contrast Suitable for semitransparent and transparent specimens. The phase structures look like hills. The effect can be strengthened or weakened by gently tilting the deflection mirror. Negative relief contrast Suitable for semitransparent and transparent specimens.
TL RCI™: Relief Images (continued) Dynamic relief contrast Suitable for semitransparent and transparent specimens. By tilting the deflection mirror slightly, you can move the gaps over the entire field of view and quickly toggle between positive and negative relief images. The dynamic effect makes it easy to distinguish phase structures from amplitude structures. Leica M series Constraints The relief methods provide good results from mid-zoom to high magnifications and with 1×, 1.6× and 2× magnifications.
Using Filters Filters for TL RC™ and TL RCI™ The transmitted-light bases TL RC™ and TL RCI™ can be equipped with up to three filters—available as accessories—simultaneously. By customer request, the filters are also available as one-off items. 2. Take the empty filter from an available filter slot in the filter holder. Daylight filter for TL ST A daylight filter is also available for the transmitted-light Base TL ST. 3. Insert the desired filter. 1.
IsoPro™ (Non-motorized): Controls Operating the cross-stage IsoPro™ 1. To move the stage in X direction, rotate the outer knob. 2. To move the stage in Y direction, rotate the inner control ring.
IsoPro™ (Motorized): Controls IsoPro X-Y Stage DCI module 1 Leica PSC Controller 1 2 2 3 1 1 2 Motorized cross-stage Leica IsoPro™ Housing with motorization Leica M series 1 2 3 4 2 3 4 Sub-D interface for Leica SmartMove™ 3 CTL2 interfaces USB interface (type B) Socket for grounded power cable Manual 1 2 3 Quick control/memory function Fine control in X direction Fine control in Y direction 89
System Illumination Leica M series Manual 90
Leica LED5000 MCI™ Use The Leica LED5000 MCI™ (for "Multi Contrast Illumination") is a universal high-output illuminator. Three groups of 3 LEDs each can be arranged as desired around the specimen. The targeted control of the LEDs enables various illumination scenarios to be implemented. Preparation Hold the MCI with both hands and pull it downwards until the illuminator clicks into place on the bottom end of the guide rods.
Leica LED5000 MCI™ (continued) Using the keyboard ★★ Use the key to switch the illumination on or off. The illumination scenarios Use the " " and " " keys to toggle between the illumination scenarios shown below. ★★ Use the "+" and "–" keys to adjust the brightness in 10 increments. Touch either of the two buttons to adjust the intensity in small increments. Hold a key to change the intensity more quickly.
Leica LED5000 RL Use The Leica LED5000 RL ("Ring Light") generates a very bright and homogenous incident light. It has a diameter of 80 mm and illuminates the specimen with 48 LEDs that can be switched on and off completely or in various combinations. It is controlled using either the integrated or via the Leica Application Suite (LAS). Using the keyboard ★★ Use the key to switch the illumination on or off. ★★ Use the "+" and "–" keys to adjust the brightness in 10 increments.
Accessories Leica M series Manual 94
Leica PSC Controller Use The Leica PSC controller gives you precision control of the Leica IsoPro™ motorized crossstage. Passing over the specimen quickly ★★ Move the joystick in any desired direction (including diagonally) to quickly control the cross-stage. Control by LAS The instructions for controlling the Leica IsoPro™ using the Leica Application Suite are provided in the software's help file. Connection Connect the Leica PSC controller and the Leica IsoPro™ motorized cross-stage.
Dimensional Drawings Leica M series Manual 96
Leica M125 / M165 C with incident light base and new focusing column (dimensions in mm) 120 120 24 455 Leica M125 / M165 C 120 120 24 455 400 292 217 277 Manual 97 0 Leica M series
431 537 Leica M125 / M165 C (continued) 154 154 356 85 106 Leica M125 / M165 C with transmitted-light base TL ST and manual focus (dimensions in mm) 430 537 431 292 217 154 85 106 154 356 Manual 30 Leica M series 340 98
547 431 Leica M125 / M165 C (continued) Leica M125 / M165 C with transmitted-light stand TL RC™ and manual focus (dimensions in mm) 154 154 95 116 385 385 Leica M series 154 95 116 154 440 390 547 431 292 217 340 Manual 99
547 431 Leica M125 / M165 C (continued) 497 Leica M125 / M165 C with transmitted-light stand TL RC™, manual cross-stage Leica IsoPro™ and manual focus 154 (dimensions in mm) 95 116 187 max. 370 300 95 116 187 249 154 497 Y = +-50 mm 547 431 292 217 max.
547 431 Leica M205 C Leica M205 C with transmitted-light stand TL ST and manual focusing drive (dimensions in mm) 154 154 90 116 370 390 547 431 292 217 370 Leica M series 154 90 116 154 340 Manual 101
547 431 Leica M205 C (continued) Leica M205 C with transmitted-light stand TL RCI™ and manual focusing drive (dimensions in mm) 154 154 95 116 385 154 95 116 154 385 Leica M series 440 390 547 431 292 217 340 Manual 102
547 431 Leica M205 C (continued) 497 187 95 116 Dimensions of Leica M205 C with transmitted-light stand TL RCI™, manual cross-stage Leica IsoPro™ and manual focus 154 (dimensions in mm) max. 410 max.
45 90 112 157 Leica LED5000 MCI 45 Leica LED5000 MCI (dimensions in mm) 45 132 83.5 36 36 112 112 22 22 132 90 90 112 112 157 157 45 45 275.
ø70 ø80 153 153 Leica LED5000 RL 30 120 Leica M series Manual 105
Specifications for the Bases Leica M series Manual 106
Transmitted-light Base TL ST Light source Quick illuminant change Illuminated area Power supply unit Connections Weight Illumination types Bright field Dark field Oblique light Relief Contrast System (RC™) CCIC (Constant Color Intensity Control) Internal shutter/lamp control Integrated filter holder Coated optics for increasing the color temperature Matching of high num.
Transmitted-light Base TL BFDF Light source Illuminated area Connections Weight External via cold light source 40 mm Connection for cold light guide, active f=10 mm, end tube f=13 mm 5.8 kg Illumination types Bright field Yes Dark field Yes Oblique light No Relief Contrast System (RC™) No CCIC (Constant Color Intensity Control) No Internal shutter/lamp control Yes* Integrated filter holder No Coated optics for increasing the color No temperature Matching of high num.
TL RC™ / TL RCI™ Light source Quick illuminant change Illuminated area Power supply unit Connections Weight Leica TL RC™ External via cold light source – 35 mm – Connection for cold light guide, active f=10 mm, end tube f=13 mm 6.0 kg Leica TL RCI™ Halogen lamp 12V/20W Yes 35 mm 100 – 240 V~, frequency 50 – 60 Hz energy consumption 30 W max., ambient temperature 10–40 °C 1×USB type A, 1×USB type B, 2×CAN-BUS 7.
IsoPro™ Motorized XY Stage Compatibility Leica incident light base and TL bases (TL BFDF, TL RC™, TL RCI™) Stage size (L × W × H) 335.5 mm × 370 mm × 41.5 mm Travel 152 mm × 102 mm (6" × 4") Speed X,Y 30 mm/s Motor resolution X,Y 0.
Appendix Leica M series Manual 111
Calculating the Total Magnification and Field of View Diameter Parameter Example MO Magnification of objective MO 1× objective ME Magnification of eyepiece ME 25×/9.5 eyepiece z Magnification changer position z Zoom position 4 q Tube factor, e.g. 1.5× for coaxial incident light, 1.6× for 45° ErgoTube™ q Coaxial reflected light 1.5×, tube factor r Factor 1.25× r Factor 1.
Care, Maintenance, Contact Persons We hope you enjoy using your high-performance microscope. Leica microscopes are renowned for their robustness and long service life. Observing the following care and cleaning tips will ensure that even after years and decades, your Leica microscope will continue to work as well as on the very first day. Warranty benefits The guarantee covers all faults in materials and manufacture. It does not, however, cover damage resulting from careless or improper handling.
Care, Maintenance, Contact Persons (continued) Protection from dirt Dust and dirt will affect the quality of your results. ★★ Put a dust cover over the instrument when it will not be used for a long time. Cleaning polymer components Some components are made of polymer or are polymer-coated. They are, therefore, pleasant and convenient to handle.
