Living up to Life User Manual Leica M125 B Leica M165 C B
Copyright All rights to this documentation are held by Leica Microsystems CMS GmbH. Reproduction of text or illustrations (in whole or in part) by print, photocopy, microfilm or other method (including electronic systems) is not allowed without express written permission from Leica Microsystems CMS GmbH. The instructions contained in the following documentation reflect state-of-the-art technology. We have compiled the texts and illustrations as accurately as possible.
Function of the Microscopes The Leica M125 B and Leica M165 C B microscopes, for which this User Manual has been written, are designed for routine examinations of cell and tissue cultures, liquids, and sediments. This includes examining specimens taken from the human body for the purpose of gaining information about physiological or pathological conditions or inborn anomalies, or testing for safety and compatibility for potential recipients, or for monitoring therapeutic measures.
General Notes Use in clean rooms The Leica M series can be used in clean rooms without any problems. Cleaning ★★ Do not use any unsuitable cleaning agents, chemicals or techniques for cleaning. ★★ Never use chemicals to clean colored surfaces or accessories with rubberized parts. This could damage the surfaces, and products could be contaminated by abraded particles.
Important Safety Notes User Manual This User Manual describes the special functions of the individual modules of the Leica M stereomicroscopy series and contains important instructions for their operational safety, maintenance, and accessories. You can combine individual system articles with articles from external suppliers (e.g. cold light sources, etc.). Please read the User Manual and the safety instructions from the supplier.
Symbols Used Warning! Safety hazard! This symbol indicates especially important information that is mandatory to read and observe. • Danger due to hot surface. This symbol warns against touching hot surfaces, e.g. those of light bulbs. Failure to comply can cause the following: ★★ Hazards to personnel ★★ Functional disturbances or damaged instruments Important information This symbol indicates additional information or explanations that are intended to provide clarity.
Safety Instructions Description ★★ The individual modules fulfill the highest requirements for observation and documentation of Leica stereomicroscopes of the M series. Intended use of instrument ★★ Leica Microsystems microscopes are optical instruments for improving the visibility of objects or specimens through magnification. Accessories such as optical accessories, stands, illumination, cameras etc. supplement the equipment configuration.
Safety Instructions (continued) Responsibilities of person in charge of instrument ★★ These Safety Instructions must be available at the workplace. Ensure that: ★★ The M series stereomicroscopes and accessories are operated, maintained and repaired by authorized and trained personnel only. ★★ All operators have read, understood and observe this User Manual, and particularly the safety regulations. Repairs, service work ★★ Repairs may only be carried out by Leica Microsystems-trained service technicians.
Safety Instructions (continued) Legal regulations ★★ Observe the generally applicable statutory and country-specific regulations for accident prevention and environmental protection. EC Declaration of Conformity ★★ Electrically operated accessories are constructed based on the state of the art of technology and are provided with an EC Declaration of Conformity.
Safety Instructions (continued) Light sources: safety regulations ★★ Light sources pose a potential risk from glare. Therefore, lamps have to be operated in closed housings and in installed condition. External power supply for the focusing column (Leica M165 C B) Permitted power supply: SINPRO SPU46-110 ★★ Never look directly into the beam path (blinding hazard). Specifications: Input: 100-240 V AC 47-63 Hz 1.2 A Output: 33 V DC 1.51 A For indoor use only.
Contents Copyright 2 Function of the Microscopes 3 General Notes 4 Important Safety Notes 5 Symbols Used 6 Safety Instructions 7 The Leica M Series Congratulations! 14 A Step Towards Infinity 15 The Electronics: Comfort, Convenience and Safety for your Experiments 16 Maximum Compatibility 17 On We Go 18 Assembly Base and Focusing Column 20 Optics carrier 21 Tube 22 Eyepieces 23 Objective 24 TL5000 Ergo transmitted light base 25 Wiring: Connections (Leica M165 C B) 26 Cables: Cable Duct 27 Lei
Optical Accessories The Different Types of Objectives 46 Bases Leica TL5000 Ergo Transmitted Light Base: Controls Leica TL5000 Ergo Transmitted Light Base: Operation 48 49 Technical Data Leica TL5000 Ergo Transmitted Light Base 52 Appendix Calculating the Total Magnification and Field of View Diameter Care, Maintenance, Contact Persons 54 55 Leica M series User Manual 12
The Leica M Series Leica M series User Manual 13
Congratulations! Congratulations on obtaining your new Leica M series stereomicroscope. We are convinced it will exceed your expectations, as never before have we applied our decades of experience in the areas of optics, mechanical engineering and ergonomics in such an uncompromising manner. The Leica M series embodies all the qualities you associate with the name Leica Microsystems: excellent objectives, high-quality engineering, and reliability.
A Step Towards Infinity Ever since their introduction by Horatio S. Greenough, stereomicroscopes have worked according to the optical principles based primarily on Ernst Abbe's research. For over a century, ingenious optics designers and engineers have worked to push magnification, resolution and image fidelity to the limit permitted by optics.
The Electronics: Comfort, Convenience and Safety for your Experiments Never before have electronics been used as extensively in a Leica series as in the new M series lineup. Optics carrier, tripod, base and illuminator are all connected using electrical contacts—which provides a number of advantages. Fewer cables A large part of the cables have been routed in the interior of the column. Data are also transferred via the interface between the column, the optics carrier and the carrier.
Maximum Compatibility Leica engineers were careful to ensure that the new Leica M series—like its predecessors— remains compatible with existing series. This means that objectives, bases, tubes and so on can be reused. Objectives All new objectives of the Leica M series are parfocal. If you prefer, you can continue to use the previous Leica objective series. In this case, parfocality is no longer guaranteed.
On We Go If your new Leica microscope has already been assembled and commissioned by your Leica consultant, click here to skip through the installation instructions and go directly to the Quick Start Guide on page 28. Leica M series If, on the other hand, you are assembling the microscope yourself, continue with the "Assembly" chapter, which begins on page 19.
Assembly Leica M series User Manual 19
Base and Focusing Column The first step is to connect the focusing column of the M series to the corresponding base. Assembling the column adapter 1. Securely install the column adapter on the column using the four included screws. Assembling the focusing drive 2. Securely screw the focusing column to the base using the six included screws.
Optics carrier Tools used ★★ Allen key, 4 mm Leica M series Assembling the Optics Carrier 1. Place the optics carrier on the focusing column so that the screw fits into the thread provided and the lug fits into the groove. User Manual 2. Press the optics carrier backwards to the focusing column and screw it in place using your other hand.
Tube All intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner. Tools used ★★ No tools required. Assembling the tube 2. Push the tube (for example, the inclined binocular tube) into the dovetail ring and rotate it slightly in both directions until the positioning screw meshes with the guide groove. 3. While holding the tube only slightly, carefully tighten the positioning screw. It is automatically brought to the correct position. Preparations 1.
Eyepieces Tools used ★★ No tools required. Magnification range You can extend the overall magnification range using available 10×, 16×, 25× and 40× widefield eyepieces for persons wearing glasses. Preparation 1. If you want to use an optional graticule, insert it now (page 44). Inserting the eyepieces 3. Push the eyepieces into the tubes as far as they will go and check to ensure that they fit tightly and accurately. 2. Remove the plastic tube guard. 4. Securely tighten the clamping screws.
Objective Tools used ★★ No tools required. Preparation 1. Remove the protective cap on the optics carrier by turning it. Attaching the objective Hold the objective firmly during assembly and disassembly so that it does not fall onto the stage plate. This applies particularly to the 2x planapochromatic objective, which is very heavy. Remove all specimens from the stage plate first. • 2. Screw the objective clockwise into the optics carrier.
TL5000 Ergo transmitted light base Unpacking the base The base is delivered with the adapter plate installed. Make sure the instruments are unpacked on a flat, sufficiently dimensioned, and non-slip surface. Focusing drive and column 1. Unscrew the extension plate from the base using the Allen key provided. 2. Attach your focusing drive column to the bottom using the 4 Allen screws. 3. Reattach the adapter plate to its original position using the 6 Allen screws.
Wiring: Connections (Leica M165 C B) The new Leica M series features extensive encoding with which various microscope data and settings can be read out and reproduced later. The terminals The connection to other instruments is made using the terminals on the rear side of the column: 1 ★★ The interface to the optics carrier is on the column. 2 Leica M series User Manual 1.
Cables: Cable Duct The integrated cable duct in the column enables a neat cable layout around the microscope. For example, the USB or FireWire cables of the camera can be stowed in the cable duct. Leica M series Feeding the cables 1. Unscrew the three screws on the cable duct. 3. Place the cables in the cable duct and screw the cover on tightly. 2. Remove the cover of the cable duct. Tip: Estimate the length of the cable ends you will need before screwing on the cover.
Quick Start Guide Leica M series User Manual 28
The Fastest Route to Success Your Leica stereomicroscope has been delivered in completely assembled condition by your Leica partner, and naturally you want to get right to work. Therefore, your next step should be to study the Quick Start Guide, which outlines the most important steps at a glance. This manual will then familiarize you with the finer details of your microscope. The following pages contain important, practical information that makes using it every day easier.
Overview of an M Series Microscope 11 1 2 3 4 5 6 7 8 9 10 Switch click stops on/off Magnification changer (zoom) Coarse/fine focusing Transmitted light base Interchangeable objective Built-in iris diaphragm Fastening screw for the binocular tube (or accessories) Adjustable eyepiece tubes Fastening screws for the eyepieces Eyepieces for spectacle wearers with dioptric correction and eyecups 11 Trinocular tube 10 9 8 7 1 2 3 6 5 4 Leica M series User Manual 30
The correct interpupillary distance The interpupillary distance is correctly set if you see a single circular image field when looking at a specimen. If you are still a novice microscope user, you may need a short time to become accustomed to this. Not to worry—after a little while, it will become automatic. Adjusting the interpupillary distance 1. Look into the eyepieces. 3. Slowly approach the eyepieces with your eyes until you can see the complete image field without corner cutting. 2.
Using the Eyepieces The eyepieces form the connection between the tube and the eye of the observer. Simply push them into the tube and they are ready to use. If you do not wear glasses: 1. Hold the eyepiece firmly and rotate the eyecups forwards counterclockwise. 2. If an eyepiece is equipped with the inte- If you wear glasses: 1. Hold the eyepiece firmly and rotate the eyecups counterclockwise towards the rear, as otherwise the viewing distance is too great.
Focusing Focusing raises or lowers the stereomicroscope using the focusing drive. The specimen detail is brought into sharp focus as soon as it is in the focal point of the objective. ★★ The focusing drive can be operated either left- or right-handed. Focusing ★★ The inner, coarse adjustment is used for covering great distances. Coarse/fine adjustment The sharpness is adjusted using the coarse/fine adjustment. ★★ The outer, fine adjustment is used for fine focusing. • load of up to 15 kg. 2.
Adjusting the Resistance of the Focus Drive Adjusting the resistance Is the focusing drive too loose or too tight? Does the equipment tend to slide downwards? The resistance can be adjusted individually depending on the equipment weight and personal preferences as follows: 1. Grip the outer drive knobs with both hands and turn them towards each other until the desired resistance is reached during focusing.
Changing the Magnification (Zoom) All M series microscopes have an integrated zoom. The name indicates the zoom range covered: Zooming 1. Look into the eyepieces. 2. Focus on the specimen. ★★ Leica M125 B = 12.5:1 ★★ Leica M165 C B = 16.5:1 3. Rotate the magnification changer until the desired magnification is configured. The rotary knob for the zoom can be used either left or right-handed.
Click Stops and Magnifications The zoom button can optionally be operated either with or without ratchet steps. Continuous zoom is possible when the click stops are disabled, which many users find convenient. On the other hand, when the click stops are enabled, photographs, measurement results etc. can be reproduced more accurately. Leica M series Enabling and disabling click stops 1. Push the top button downwards to disable the ratchet steps. 2. Push the bottom button upwards to enable the ratchet steps.
Parfocality: More Comfort and Convenience for Your Work All Leica stereomicroscopes are parfocally matched, meaning that you can view a focused specimen from the lowest to the highest magnification without having to refocus. The focus needs to be readjusted only if you want to view a specimen detail that is located higher or lower. Parfocality 1. Enlarge the view to the maximum level.
Iris Diaphragm The iris diaphragm in the optics carrier of your M series microscope has the same purpose as those in a camera shutter: it regulates the available light, which changes the depth of field. The "depth of field" (or "depth of focus") is the area of a specimen that is brought into sharp focus. Leica M series Closing the iris diaphragm ★★ Close the iris diaphragm by turning the knob to the left. The subject appears darker and the depth of field increases.
Eyepieces Leica M series User Manual 39
Magnification Factor of the Eyepieces An eyepiece not only makes it possible to look passively into the microscope, but also has a critical effect on the maximum magnification. The magnification factor is between 10x and 40x.
Health Notes Potential sources of infection Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the eye. The risk can be kept to a minimum by using individual eyepieces or detachable eyecups. Eyecups can be ordered separately. Please contact your Leica partner. • Separate eyecups are an effective way of preventing infections.
Dioptric Correction All Leica eyepieces are also available with builtin dioptric correction, allowing the microscope to be used without glasses even by those with vision problems. The correction comprises ±5 diopter settings. Using the Dioptric Correction 1. Set the dioptric correction of both eyepieces to the mid position ("0" diopter settings). 2. While wearing your glasses, look through the eyepieces and focus on the specimen.
Dioptric Correction and Parfocality Leica stereomicroscopes are parfocally matched. The prerequisite for this is the correct setting of the diopters and the parfocality. The following adjustments have to be carried out only once by each user. Adjusting 1. Set the dioptric correction for both eyepieces to "0". Preparations ★★ Move the lever of the video/phototube to the "observation" position and open the diaphragm. 3. Select the highest magnification and readjust the sharpness. 2.
Graticules Use Leica graticules make counting easier, particularly for workstations that are not equipped with a digital camera. 2. Clamp the graticule on the insert, applying moderate pressure. Ensure that the graticule fits tightly. 4. You can now align the graticule by rotating the eyepiece in the tube and then tightening it using the clamping screw. The Leica graticules for length measurements and numbering are fitted in mounts and are inserted into the eyepieces. 1.
Objectives and Optical Accessories Leica M series User Manual 45
The Different Types of Objectives To meet the various requirements regarding imaging properties, there is a choice of highquality interchangeable planachromatic and planapochromatic objectives and also lowerpriced interchangeable achromatic objectives. ★★ Achromatic objectives are particularly suited for specimens with high-contrast structures. ★★ Flat-field (planachromatic) objectives are particularly well suited for studying flat objects such as wafers and thin sections. Achromatic objectives The 0.
Bases Leica M series User Manual 47
Leica TL5000 Ergo Transmitted Light Base: Controls 1 2 Rear side of the TL5000 Ergo 1 2 Power switch for the base Power supply connection 1 2 3 45 Left side of the TL5000 Ergo 1 2 3 4 Refer also to the separately provided User Manual for the TL5000 Ergo transmitted light base. Leica M series 5 LED display for opening and positioning the aperture or for balance in DF mode.
Leica TL5000 Ergo Transmitted Light Base: Operation The transmitted light base must only be connected to a grounded socket with a faultless power cable! Failure to observe these warnings may result in serious personal injury or even death! Switching the transmitted light base on and off 1. Switch on the transmitted light base with the power switch on the rear side. 2. Press the on/off switch on the right side once to turn on the illumination.
Leica TL5000 Ergo Transmitted Light Base: Operation (Continued) 3. Look through the eyepiece and adjust the light intensity using the control on the right side. The intensity of the illumination is visualized by the LED scale. 4 The following assumes that the power switch on the rear side of the device is always switched on. This switch is not mentioned in the remaining part of this User Manual. Press the on/off switch on the right side once again to turn off the illumination on the base.
Technical Data Leica M series User Manual 51
Leica TL5000 Ergo Transmitted Light Base Light source Light source Illuminated field: Bright field ∅ Illuminated field: Dark field ∅ Relief Contrast System (RC™) Internal shutter/lamp control Integrated filter holder Matching of high num. aperture Remote control options AntiShock™ Pads Dimensions (W×H×D) Power supply Input Output Connections Power supply Leica M series LED 65 mm 40 mm Yes Yes Yes Yes Yes Yes 412×341×46 mm 100–240 VAC 47 – 63 Hz 3.2 A 33 VDC 3.
Appendix Leica M series User Manual 53
Calculating the Total Magnification and Field of View Diameter Example Parameter MO Magnification of objective MO 1× objective ME Magnification of eyepiece ME 25×/9.5 eyepiece z Magnification changer position z Zoom position 4 q Tube factor, e.g. 1.5× for coaxial incident light, 1.6× for 45° ErgoTube™ q Coaxial incident light 1.5×, tube factor r Factor 1.25× r Factor 1.
Care, Maintenance, Contact Persons We hope you enjoy using your high-performance microscope. Leica microscopes are renowned for their robustness and long service life. Observing the following care and cleaning tips will ensure that even after years and decades, your Leica microscope will continue to work as well as it did on the very first day.
Care, Maintenance, Contact Persons (continued) Protection from dirt Dust and dirt will affect the quality of your results. ★★ Put a dust cover over the microscope when it will not be used for a long time. Cleaning polymer components Some components are made of polymer or are polymer-coated. They are, therefore, pleasant and convenient to handle. The use of unsuitable cleaning agents and techniques can damage polymers.
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