Leica ST 5020 Multistainer Instructions for Use Leica ST5020 V1.9 English - 12/2012 Order No. 14 0475 80101 RevD Always keep this manual together with the instrument. Read this Instructions for Use carefully before working with the instrument.
NOTE The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state-of-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual. For erroneous statements, drawings, technical illustrations etc.
Table of Contents 1. Important information ................................................................................................................ 6 1.1 1.2 1.3 1.4 2. Safety ............................................................................................................................................ 7 2.1 2.2 3. Installation site requirements ......................................................................................................... 17 Installing the instrument ...
Table of Contents 5.4.4 5.4.5 5.4.6 5.4.7 5.4.8 5.4.9 5.4.10 5.5 5.5.1 5.5.2 5.5.3 5.5.4 5.6 5.6.1 5.6.2 5.6.3 5.7 5.8 5.8.1 5.8.2 5.8.3 5.8.4 6. Cleaning and maintenance .................................................................................................... 75 6.1 6.2 6.3 7. General ............................................................................................................................................... 78 Correcting errors .............................................
1. Important information 1.1 Symbols used in this manual and their meaning Warnings appear in a grey box and are marked by a warning triangle . Notes, i.e. important user information appear in a grey box and are marked by an information symbol . Flammable solvents and reagents are marked with this symbol. Instrument surfaces which become hot during operation are marked with this symbol. (5) Figures in brackets refer to item nos. in drawings.
2. Safety Make sure to comply with the safety instructions and warnings in this chapter. Make sure to read these instructions, even if you are already familiar with the operation and use of other Leica products. 2.1 Safety instructions This Instructions for Use includes important information related to the operating safety and maintenance of the instrument and it is an important part of the product.
2. Safety Warnings - Markings on the instrument itself Markings on the instrument showing the warning triangle indicate that the correct Instructions for Use (as defined in this manual) must be followed when operating or replacing the item marked. Failure to adhere to these instructions may result in an accident, personal injury, damage to the instrument or accessory equipment. Some instrument surfaces, which become hot during operation are marked with this warning label.
2. Safety Warnings - Operating the instrument The Leica ST5020 may only be operated by trained laboratory personnel, according to its designated use and per the present Instructions for Use. In case of emergency switch off mains and unplug the power chord. While working with reagents (filling / emptying the reagent stations, working on the instrument while the lid/s is/are open) appropriate protective gear (lab coat, gloves, safety goggles) must be worn.
3. Instrument components and specifications 3.1 Overview - Instrument components Activated carbon filter Transfer arm Single (Double) carrier Oven modules Lid Wash stations Reagent container Color touch screen Mains switch Transfer to Coverslipper Leica CV5030 (optional) Load drawer Exit drawer Slot for PCMCIA card Fig. 1 10 Instructions for Use, V1.
3. Instrument components and specifications Rear of instrument Fan electronic Data connections Power supply inlet (electronic) Power supply outlet Mains Power supply inlet Transfer to Coverslipper (optional) Secondary fuses Drain hose Cover for Oven-Modules adjustable foot Drain outlet Exhaust air duct DI-Water inlet Station 7 Water inlet Stations 8-12 Fig.
3. Instrument components and specifications 3.2 Instrument specifications • • • • • • • • • • • • High specimen throughput (at maximum 12 racks simultaneously) Simultaneously processing of multiple different programs Color TFT-Touch screen Graphical and intelligent user Interface Context-sensitive On-line Help Graphical process representation on color screen. Reagent Management System. Multilingual. Maximum of 40 stations. Load drawer with max. 4 stations. Exit drawer with max. 4 stations.
3. Instrument components and specifications 3.3 Standard delivery - packing list The Leica ST5020 standard delivery consists of the following items: 1 Leica ST5020 basic instrument 31 Reagent vessels, assy. (with handles and lids) 5 Wash vessels, assy. 2 Activated carbon filter 1 Set of power cords: 1 Power cord "D" 1 Power cord "UK" ST-BU F-5A 1 Power cord "USA-C-J" 1 Accessory-Kit consisting off: 1 Jumper cable - mains 3 Reagent vessels, assy.
3. Instrument components and specifications 3.
3. Instrument components and specifications Dimensions and weight Basic instrument (W x D x H), in mm: Dry weight, unpacked: Weight, packed: 1060 x 750 x 540 ca. 90 kg ca.
3. Instrument components and specifications 3.5 Container system 6 1 Reagent container The Reagent containers can be individually removed for filling. For use, fill the reagent containers to the line marked on the inside (approx. 450 ml capacity) and place into position in the instrument consistent with the programs you wish to run. Ensure that the reagent containers are correctly seated and that the handles (1) are over to the side and will not obstruct slide rack movement.
4. Setting up the instrument 4.1 Installation site requirements • Stable, exactly horizontal laboratory bench with even surface of a minimum width of 1.60 m and minimum depth of 0.80 m. • Tap water supply located a maximum of 2.50 m and waste water drain pipe a maximum of 2.00 m away from the corresponding in-/outlets at the rear panel of the instrument. • If the instrument is to be operated with air evacuation hose, a fume cupboard at a distance of maximum 3.50 m from the instrument is required.
4. Setting up the instrument 4.3 Tap water supply connection Water filter In addition to the filter sieve supplied (item 3 in Fig. 6) Leica recommends installing a sediment filter between the water extraction outlet and ST5020, to guarantee that the instrument is only operated with clean water. This sediment filter should have the following properties: Filter fineness: 25 μm Rate of flow: approx.
4. Setting up the instrument • Screw tap water inlet hose (7 in Fig. 8) for tap water stations 8 - 12 onto water tap and insert the other end into inlet (8 in Fig. 7) . Instrument rear panel - tap water inlets 8 • If station no. 7 (Fig. 3) will also be used as a tap water station, connect a second tap water inlet hose (optional accessory) to inlet (9). 9 10 Fig. 7 Inlet (9) only provides water (or any other medium supplied via a separate connection) to station no. 7.
4. Setting up the instrument Connecting the waste water hose • Outlet connection piece (10) can be turned to point into different directions, depending on whether the drain water pipe is located to the left or to the right of the instrument. 7 13 • To adjust the connection piece, loosen screw (11) of hose clamp (12) sufficiently so that the connection piece (10) can be turned. Adjust the connection piece as desired (always with the opening pointing slightly downwards) and retighten screw (11).
4. Setting up the instrument • Take the oven kit out of the box and verify whether it is complete. 4.4 Installing the oven (optional) The following parts must be included: 15 20 19 21 15 - Oven module 16 - Paraffin collecting tray 17 - Cover 18 - Screws 19 - Washers 20 - Screwdriver 21 - Allen key, size 2.5 22 - Allen key, size 4.0 18 22 17 Insert paraffin collecting tray into corresponding opening in oven module (possible only in one direction, as shown here). 16 Fig.
4. Setting up the instrument Important! Prior to taking any further installation steps, switch the instrument off and unplug from mains. 27 • Cover panel (26) must be removed from the right inner wall of the instrument, so that the transfer arm can access the oven stations. 26 27 • To do so, remove six screws (27) (Allen key, size 2.5) and remove cover panel (26). • Store screws and cover panel in safe place for further use. Fig.
4. Installing the oven (continued) Setting up the instrument Important! If only one oven is installed, it must be located on the right side (seen from the rear of the instrument). 31 • Place oven on rails (32) and insert. 32 • Keep pushing until the connection pieces of the oven (positioning pin (24), connector strip (25), see Fig. 11) lock into the corresponding connections (31) in the instrument. Fig.
4. Setting up the instrument If the instrument is operated without activated carbon filter, the air evacuation hose must be installed instead. The air evacuation hose is attached to fan housing (68) located at the rear left of the instrument. Unpack the components supplied and check for completeness. The package must contain the following items: 60 - Screw driver 61 - Allen key, size 2.5 62 - Hose clamp 63 - Allen screw with washer 64 - Hose connection piece with seal 65 - Air evacuation hose 4.
4. Setting up the instrument 4.6 Inserting the activated carbon filter 34 51 Important for trouble-free operation: The sealing profiles (33) must fit against the rear wall of the filter housing (34). Filter - inserted 33 35 Fig. 18 Make sure to insert the filter with the correct side pointing upwards: the three arrows on the label (51) located at the right of the filter must point to front. Fig.
4. Setting up the instrument Adjustable instrument feet Bring the instrument to an exact horizontal position by screwing the adjustable feet in / out as needed. To do so, proceed as follows: • Remove all vessels located in stations 1 - 24 so that the vessel holder frames can be used to support the box level for measuring purposes. • All four instrument feet (37) are height-adjustable. To adjust, place single head wrench, size 13 (standard delivery) onto hexagon bolt (36) and adjust each foot as needed.
4. Setting up the instrument 4.8 Electrical connection The instrument MUST be connected to an earthed mains power outlet socket. The instrument is supplied with a set of country-specific power cords. Make sure to use the appropriate mains cable for the local voltage supply (wall outlet). Sockets for mains power supply and data transfer. Secondary fuses.
4. Setting up the instrument 4.9 Alarm functions Instrument alarm The instrument alarm is generated from within the Leica ST5020. The instrument alarm is used for all alarm situations. Remote alarm This alarm is external to the Leica ST 5020. Any alarm generated in the instrument, no matter of which alarm type, is passed on to the remote alarm system, even when the type 1 instrument alarm is switched off.
5. Operation 5.1 Switching the instrument on Caution! The instrument MUST be connected to an earthed mains power outlet socket. It is recommended that the Leica ST5020 be plugged into a wall socket that has Ground Fault Circuit Interruption (GFCI) protection - as an additional electrical safeguard. • Before you connect the instrument to the wall socket, make sure the mains switch at the bottom right of the instrument is in position OFF (‘0’).
5. Operation 5.2 Setup checklist Once the instrument is ready to be switched on, all menus listed below must be accessed and the necessary parameters be properly selected. The Supervisor mode must be active to have access to all menus. For detailed advice on how to select individual parameters, refer to the on-line help feature. → Press button Screen → → ↓ Select a language - either when switching the instrument on or in the LANGUAGE CHOICE menu. In USER INTERFACE, set parameters as required.
5. Operation 5.3 Touch screen functions 5.3.1 User interface - overview The Leica ST5020 Multistainer is programmed and operated via a colored TFT- touch screen. Basic screen layout Only those control elements that can actually be accessed by the user are displayed on the screen. The number of accessible control elements depends on the access level selected.
5. Operation Button symbols The six buttons described below have the same function in all menus. On the left side of the button bar (Fig. 26) there will either be the button BACK → To go back to the previous menu. or the two buttons Enabled Disabled → 32 CONFIRM → CANCEL → PRINT → Displayed in menus containing tables and lists. If pressed, the list selected will be printed immediately. Immediate printing does not affect the daily printout of all protocols (see chapter 5.4.6).
5. Operation Tables/lists 1 Fig. 27 The program contains numerous tables. The first row of a table contains the headers of the individual columns. The rows below contain the actual content. Selected rows appear in blue color (1). When a tabled is accessed, the blue bar is always located in the first content row. If a table is empty, no blue bar is displayed. Headings of column where parameters can be entered by the user (in supervisor mode) (Fig.28) are displayed as buttons (3).
5. Operation 5.3.2 Data entry Parameters and data can be set / entered several different ways: Alphanumeric keyboard 4 5 Fig. 29 The alphanumeric keyboard (Fig. 29) resembles a computer keyboard. An entry field (5) and instructions on what kind of data to enter (4) are located above the keyboard. The amount of characters that can be entered varies, depending on the type of data to be entered.
5. Operation Navigation buttons The navigation buttons have the same function in both keyboard types (numerical, alphanumeric): • Cursor jumps to the left / right border of the entry field. • Cursor is moved one character to the left / right. • Character on the left of the cursor is deleted. Numerical keyboard 6 • Entry field located above the numeric keypad (left aligned). Instructions on which parameter to be entered (6) are displayed above the entry field.
5. Operation The Leica ST5020 may be configured to allow two level of user access. User access level • Users may run programs and view results. On this level, the USER symbol is displayed in the upper right corner of the touch screen. 5.3.3 Access levels Administrator symbol User symbol Fig. 31 → In SUPERVISOR mode, NO programs can be run. Switch to USER mode to start a program. You can only go back to SUPERVISOR mode (e.g. to select / change settings) once all running programs have been finished.
5. Operation 5.3.4 The main menu After switching the instrument on, the main menu will be displayed only if the language is changed. INSTRUMENT STATUS is actually the standard screen. In all functional descriptions below, INSTRUMENT STATUS is assumed to be the standard screen, all buttons shown start from INSTRUMENT STATUS. Opens up the main menu from where all program functions can be accessed. The main menu can be accessed directly from almost all functions.
5. Operation 5.
5. 5.4.1 User interface → Operation → In this screen you can select: • BRIGHTNESS OF SCREEN • TEMPERATURE MEASURING UNIT • BUTTON CLICK Two measuring units can be chosen from: • The parameter selected is displayed on the button, next to the “=“ sign. ←→ Fig. 34 • Press the button to switch to the alternative measuring unit. ↓ Language selection To select between the two languages that are available, one of these two languages always being ‘English’.
5. Operation 5.4.2 Alarm → → → There are three different types of alarms, each one allocated to certain events. Alarm type 1 can be simply switched off, alarm types 2 and 3 cannot. Alarm type 1: Alarm 1 is generated when user intervention is required. Alarm type 2: An error has occurred or user intervention is urgently required. Fig. 36 Alarm type 3: A serious error has occurred. Follow the instructions provided by the error message.
5. Operation The table below shows the specific events each individual alarm type is allocated to. Time/Location Alarm type Event Alarm ceases / switched off Alarm 1 All unload containers occupied when instrument is - When all racks have been removed. switched on. Alarm 3 Switching on after power failure while staining procedure was in progress. - When dialog box is acknowledged. Alarm 3 Load/unload drawer open - time limit exceeded. - After drawer has been closed.
5. Operation 5.4.3 Password allocation → → → Any changes in this menu require supervisor access level. You may switch to supervisor mode only while no racks are being processed. Switching password verification on/off When switching between user and supervisor mode, switch the password verification on or off: • Press PASSWORD VERIFICATION. • The alphanumeric keyboard opens up - enter the password.
5. 5.4.4 Set date/time → Operation → Many of the instrument functions are time-controlled. - therefore it is very important to set date and time correctly. Date The DATE / TIME window displays the current calendar month. • To set the DAY press the corresponding button. • Set MONTH and YEAR using the arrow buttons. • After having set the date, press CONFIRM to exit. Fig. 38 Time • To set the time, press TIME - the TIME window opens up. • Use the arrow buttons to set HOURS and MINUTES.
5. Operation 5.4.5 Movement → → Any changes in this menu require administrator mode. For this reason, changes can only be performed if no staining programs are active. The parameters set in MOVEMENT are valid for all programs. For • NUMBER OF DIPS • DIP AMPLITUDE • LIFTING SPEED only the settings indicated on the buttons can be selected. The currently selected setting shows on the button next to the “=“ symbol. Press the button to go to the next higher setting. Fig.
5. 5.4.6 View and print → Operation → To view all stored protocols. The button bar of each protocol contains a PRINT button for individual printouts of each protocol. ↓ Fig. 41 • Printing is possible only when a printer is connected. • Pressing the PRINT button starts printing the selected list immediately. If printing is not possible, the button is disabled. • Press PRINT ALL to immediately print all six protocols. Each protocol will start on a new page.
5. Operation 5.4.7 Reagent list → → The reagent list is an integral part of the user interface and can therefore be accessed from several different menus. Adjust it to the current reagents as soon as possible, as this makes programming considerably easier. Fig. 43 • There is a default list of standard reagents containing all reagents needed for the most common staining methods. • All reagents are listed in alphabetical order. A blue background indicates that a reagent has been selected.
5. Setting RMS data Operation If the Reagent Management System (RMS) is switched on, the table headers are displayed as buttons. The following limit parameters can be defined for the RMS: • Maximum number of days the reagent may be used. • Maximum number of racks that can be processed until the next reagent change. ↓ ↓ Fig. 44 In ‘instrument status’ a red bar in the station button indicates how long a reagent has already been used depending on the values entered here.
5. Operation The RMS log → → → → The Leica ST5020 is equipped with a Reagent Management System (RMS) graphically representing processing history of reagents in ‘Instrument Status’ (see chapter 5.5.1). These reagent data are stored in the RMS log table. The RMS log table lists all reagent stations, the reagents allocated to each station as well as their processing history. Last change Date when the reagent was last changed.
5. 5.4.8 Station options → Operation → This screen provides an overview of all stations. Detailed information on individual stations can be retrieved. Rack movements, however, are not shown. 8 Fig. 46 • Screen indication and functionality of stations are identical to the INSTRUMENT STATUS screen (see Fig. 49). • Pressing a station button opens up the STATION DETAILS menu (Fig. 51). • Programs ready to be started are displayed above the station buttons (8).
5. Operation • PRINT button: Press for a printout of the current station configuration. Instead of the graphical screen display, a list (Fig. 47) will be printed, which, in addition to the information provided by the graphical screen, also shows the reagents allocated to each station. Fig. 47 5.4.9 Calibration dialog → In the CALIBRATION DIALOG menu you can enhance or restore touch screen response to buttons being pressed. We recommend to use a pen for the calibration procedure.
5. 5.4.10 Data backup → Operation → This menu allows all instrument data to be written to a PCMCIA card. It is even possible to load saved data from such a card into the multistainer memory. The data can only be backed up in supervisor mode (9, fig. 48). If a staining program is running, it is therefore impossible to write data to a PCMCIA card or load data from the card into the memory. To ensure compatibility of card and instrument, the card must be formatted IN THE INSTRUMENT before its initial use.
5. Operation Important! At least 1 MB of free memory is required on the PCMCIA card. Otherwise, data backup cannot be completed and the writing of the data to the card will appear to take a very long time. In this case (if data backup lasts longer than 2 min.), remove the card from the slot, replace it with an empty card and repeat data backup. Backup the data as follows: ↓ ↓ • Insert a PCMCIA card into the card slot beside the mains switch (see fig. 1, page 10).
5. 5.5 Instrument status Operation → INSTRUMENT STATUS is the standard screen of the instrument, displayed automatically when the instrument is switched on (unless a different language is selected - s. chapter 4.1). • INSTRUMENT STATUS may also be accessed from the main menu, in which case a BACK button will be displayed in the menu bar. • The graphical display provides information on station configuration, reagent status and current location of racks during a program run.
5. Operation 5.5.1 Station buttons Each of the 40 stations is configured as a button that can be pressed to directly access the STATION DETAILS menu. The station buttons are divided into three lines containing different types of information, depending on each individual configuration. 3 2 4 5 5.5.2 Station types • The first line contains the station number (2) plus a letter (3) indicating the station type.
5. Operation Unload station • There is a maximum of four unload stations (E25 - E28) - E25 being nonchangeable. Unload stations do not have a bar on the left border. • Completed racks are placed into the unload stations by the transfer arm, always starting with station E25. Line 2 shows which program has been completed, line 3 shows the rack number. The colored program indication blinks in two-second intervals.
5. Operation 5.5.3 Select station type ↓ 10 9 8 ↓ ↑ Fig. 52 Fig. 53 Station details • Press one of the station buttons to access the STATION DETAILS menu. • If a station is empty, only the CHANGE STATION TYPE button (8) is displayed. • If CHANGE STATION TYPE is pressed, the menu STATION TYPE CHOICE (Fig. 53) opens up where you can see which station types can be allocated to a particular station. Buttons corresponding to station types which cannot be selected are disabled.
5. Operation Indications and buttons corresponding to the different station types Reagent station Additional buttons To open the reagent list (chapter 5.4.7) for allocating reagents to stations. Press UPDATE REAGENT after a reagent has been changed, in order to • set the date of “Last change“ to the current date; • set the date in the “Use by date“ row according to the RMS; • Set the rack counter to “00“. Additional indication Reagent name: Shows the reagent allocated.
5. Operation 5.5.4 Change station type Load/unload station → Reagent station • Load stations L33 - L35 and unload stations E26 - E28 can be defined as reagent stations, if needed. Fig. 54 5 ↓ • Both station types must be redefined station by station (one station at a time), starting from the ‘center’; i.e., if you wish to convert load and/or unload stations into reagent stations, you must start by converting stations E28 or L33, respectively.
5. 5.6 Staining programs Operation → The PROGRAMS menu includes a table listing all staining programs stored. In supervisor mode (Fig. 54) the column headers CLIP COLOR, ABBR. and PROGRAM NAME are displayed as buttons. In addition, the buttons DETAILS, COPY, DELETE and NEW are displayed, i.e. programs can be edited in this screen. • Up to 50 different staining programs can be entered and stored. • Existing programs may be copied and/or edited.
5. Operation 5.6.1 Creating programs ↓ Creating new programs • Press NEW (see Fig. 55) to create a new program row. • Press PROGRAM NAME in the table header and enter a new program name via the KEYBOARD. • Press ABBR. to allocate a 3-digit code to the new program. This code will be displayed in the INSTRUMENT STATUS and STAINING SCHEDULE menus. • Press CLIP COLOR to access the PROGRAM CLIP COLOR ASSIGNMENT menu. • Press a color button to allocate a color to the program selected.
5. Operation 5.6.2 Defining the program steps • Press DETAILS (Fig. 54) to access the PROGRAMMING menu (Fig. 56). If a program is created from scratch, the list of the individual program steps is completely empty. • The number in the STEP column indicates the order in which the stations will be used. Press INSERT ROW - a new program row (step) is created in the row below and highlighted in blue/red. ↓ 1 2 Fig.
5. Operation Defining the program steps (continued) Selecting a station for a step • Click on STATION in the header row, the CHOOSE STATION menu opens up (Fig. 58). • To allocate a station to the step, press the corresponding station button. The station (button) is displayed as pressed, and the lower row shows “P 1“. ↓ Fig. 58 • Pressing CONFIRM assigns the selected station to the step, and the display switches to the PROGRAMMING menu (Fig. 60).
5. Operation Assigning reagents • If no reagent is assigned to a program step, press REAGENT in the header row to switch to the reagent list (see chapter 5.4.7). Highlight one of the reagents in the list and press CONFIRM to assign it to the program step. ↓ Fig. 61 ↓ 1 • The reagent is now assigned to the step but not yet to the station in which the step must be executed. For this reason, the row is highlighted in red (1) indicating that the program step is not yet ready to be started.
5. Operation Defining exact steps As there is potential for timing clashes to occur when multiple racks are present in the instrument, steps which require precise timing are designated as ‘exact’ in the program. • Press EXACT to alter between yes (=exact) and no (not exact). • Immersion times at exact steps are achieved to within ± 1 second.
5. Operation Completing the program • Once the parameters EXACT and DIP have been selected, the definition of your step is complete. • Press INSERT ROW to create a new row for the next program step and once again define all step parameters as described above. • Keep creating program steps until your program is complete. • Program steps whose definition is not correct (duplicate station assignment, incorrect reagent,...) are highlighted in red in the REAGENT column.
5. Operation 5.6.3 Assigning a clip color In this menu, you tell the instrument what color the clips attached to the individual racks are. You need to be in supervisor mode to assign colors to clips. Automatic start Every time the load drawer is closed, the loading area is scanned for transponder signals coming from the clips attached to the racks in that area. • In supervisor mode, the instrument automatically switches from INSTRUMENT STATUS to CLIP COLOR if an unknown clip code is detected on a rack.
5. → → ↓ Operation Manual color allocation Go to OPTIONS to assign a new color to a clip already linked to a different color. Example: A purple clip currently not needed needs to be defined as ‘white’, as a white clip is needed but currently not available. Proceed as follows: • Place a rack bearing the clip the color of which you want to redefine into any one of the load stations and close the drawer. Fig. 64 • The clip is now identified via the transponder signal.
5. Operation 5.7 Programs in process → or → PROGRAMS IN PROCESS is a table listing all programs currently in progress. It can be displayed instead of instrument status, as PROGRAMS IN PROCESS also provides information on all important run times. The table contains the following information: Rack Rack number - as in instrument status. Staining protocol 3-Digit code and clip color allocated to a program. Station Indicates current rack position. GA means that a rack is currently being transferred.
5. 5.8 Staining Prior to starting a staining program, thefollowing preparatory steps must be taken: • Optimize all USER INTERFACE parameters. • Programs needed for staining must be ‘ready-to-start’. • Stations have to be configured and filled according to configuration. • Racks needed for staining must be equipped with a clip and filled with the slides to be processed. Place the clips (1) onto the rack with the transponder (2) facing outwards.
5. Operation 5.8.2 Starting programs → • Once the drawer is closed, the load stations are scanned in order to identify the clips on the stations that have been loaded. 4 • Once identified, the clip colors / program codes are displayed in INSTRUMENT STATUS (3) (see also chapter 5.5). Automatic program start • Once all components have been identified and are ready to be started, the staining programs will start automatically.
5. Operation Loading an unknown clip • If a rack carrying an unknown clip is placed into the load drawer, the CLIP COLOR menu opens up automatically. If any staining processes are already in progress, a screen prompt will open up inquiring whether the user wishes to access supervisor mode. - CONTINUE stops the selection procedure and continues the staining processes in progress.
5. Operation A staining process can be interrupted: • to gain access to the instrument in case any problems occurring during staining require user intervention. • to check or change reagents. • Press PAUSE in INSTRUMENT STATUS to interrupt all staining programs in progress at any given time. 5.8.3 Interrupting a staining program The PAUSE button is displayed only when the instrument is in user mode and at least one staining program is being run. Fig.
5. Operation 5.8.4 Finishing programs Unloading racks from the unload drawer If a rack is located in the unload drawer, the alarm type 1 sounds. • The code of the completed program and (in the bottom row) the basket number are displayed in the unload station symbol. The colored program indication blinks in two-second intervals. ↓ • Carefully open the unload drawer and remove the rack. You may also remove the entire reagent container together with the rack and replace it by another container.
5. Operation Cancelling racks Racks can be cancelled if needed. ↓ • Press STAINING SCHEDULE to access the staining schedule menu. • Select the rack you wish to cancel (highlight with blue bar). • Press CANCEL RACK. • Press YES to confirm. If NO is pressed, the instrument will switch back to the staining schedule menu without any changes being made. Fig. 71 ↓ ↓ • When pressing YES a second prompt opens up, where you need to confirm that you have removed the rack from the station it was located in last.
6. Cleaning and maintenance 6.1 Cleaning the instrument Prior to cleaning the instrument, always switch off mains and unplug the power cord! When handling cleaning detergents, follow the instructions of the manufacturer and make sure all laboratory regulations in force in your country are complied with.
6. Cleaning and maintenance Wash and reagent vessels Clean the wash and reagent vessels in the laboratory dishwasher at maximum +65 °C. Use a standard laboratory dishwasher detergent. The vessels may not be exposed to temperatures any higher than 65 °C (e.g. do not clean in industrial dishwashers, which run at a temperature of 85 °C), as the stations might become deformed! Drain outlet / hose From time to time check the waste water drain hose for accumulated dirt, particularly for algae.
6. Cleaning and maintenance 6.3 Preventative maintenance schedule Maintenance activity daily Clean the TFT screen with a lint-free cloth. Follow the manufacturer's instructions when using screen cleaner. √ Clean outside surfaces of instrument and lid with soft cloth. Use cleaning detergent, if necessary. √ Clean wash vessels. Check the wash vessel O-rings for damage and lubricate them slightly. See Fig. 4, page 16 for further details. weekly monthly √ Remove and clean the reagent vessels.
7. Troubleshooting 7.1 General The Leica ST5020 is equipped with a troubleshooting system. If an error is detected, detailed error messages and corresponding user instructions on what to do are displayed on the touch screen. For all correctable error conditions the instrument will take corrective actions to protect the specimens. Details of all staining protocols carried out are recorded in the daily run log. The daily run log can be viewed and/or printed in the VIEW/PRINT menu. 7.
7. Troubleshooting Power failure Malfunction / failure • Instrument not working - no response. • No data can be entered via touch screen or touch screen darkened. • Stepper motors do not work. • Instrument does not work properly. • Instrument cannot be operated at all or instrument responses are faulty. • Erroneous display indication. Leica ST5020 Fuse to be checked Fuse F1 Fuse F2 • Wash stations emptying (solenoid valves not being addressed). • Extraction fan not working. • Transfer arm not moving.
7. Troubleshooting 7.4 Replacing the secondary fuses Prior to exchanging a fuse, always switch the instrument off and unplug the mains cord. Defective fuses may be replaced only with the replacement fuses supplied together with the instrument. Important! Follow the instructions below to make sure you select the correct replacement fuse for each fuse holder.
7. Troubleshooting Replacing the fuses 1 3 2 2 Fig. 73 Fig. 74 • Insert a screwdriver (1) into the slot on fuse holder (2) and push slightly inwards rotating the screwdriver 1/4 turn to the left at the same time. • Remove the defective fuse (3) from the fuse holder (2) and insert the correct type replacement fuse. • The fuse holder is released and can be removed. • Reinsert the fuse holder together with the fuse into the instrument housing.
8. Warranty and service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted. The scope of the warranty is based on the content of the concluded agreement.
Leica ST5020 Reagent Station 27 Exit Reagent Station 14 Reagent Station 26 Exit Reagent Station 13 Reagent Station 25 Exit Reagent Station 15 Station 3 Station 2 Station 1 Staining: 9.
Reagent Reagent Station 14 Reagent Reagent Station 13 Reagent Station 27 Exit Station 26 Exit Station 25 Exit Reagent Station 15 Station 3 Station 2 Station 1 Staining: Container Map Exit Station 28 Reagent Station 16 Reagent Station 4 Station 30 Reagent Reagent Reagent Station 18 Reagent Station 6 Station 29 Reagent Station 17 Reagent Station 5 Reagent Station 31 Reagent Station 19 Reagent Station 7 Reagent Station 32 Reagent Station 20 Water Station 8 Load
9. Appendix 9.2 Optional accessories To prevent damages to the instrument and specimens, only those accessories and spare parts which have been authorized by Leica may be installed or used with the instrument. Reagent vessels, assy. (with handles and lids) Wash vessels, assy.
9. Appendix Optional accessories Order No. Power cord "D" Power cord "UK" ST-BU F-5A Power cord "USA-C-J" Jumper cable - mains 04 Vessel holder, assy. Hose clamp for waste water hose Waste water hose 2 m Waste water hose 4 m Exhaust air hose 2m assy. Exhaust air hose 4m assy. Remote alarm jack - 6.3 mm Oven - Leica ST5020, assy. Leica TS5025 - Transfer station, assy.
10.
Notes 88 Instructions for Use, V1.
