Instructions for Use Leica SM2010 R Sliding Microtome Instructions for Use Leica SM2010 R, V 1.4 RevC – 09/2013, English Order No.: 14 0508 80101 RevC Always keep this manual with the instrument. Read carefully before working with the instrument.
NOTE The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state-ofthe-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.
Table of contents 1. Important Information.............................................................................................................................. 3 2. Safety.......................................................................................................................................................... 4 2.1 2.2 3. Instrument Components and Specifications........................................................................................ 7 3.1 3.2 4.
1. Important Information Symbols in the text and their meanings Warnings appear in a gray box and are marked by a warning triangle . Notes, i.e. important user information appears in a gray box and is marked by an infor. mation symbol (5) Numbers in parentheses refer to item numbers in illustrations. Manufacturer Date of Manufacture This product fulfills the requirements of the Council's Directive 98/79/EC concerning in vitro diagnostics (IVD) medical devices.
2. Safety Be sure to comply with the safety instructions and warnings provided in this chapter. Be sure to read these instructions, even if you are already familiar with the operation and use of other Leica products. 2.1 Safety notes These Instructions for Use include important information related to the operating safety and maintenance of the instrument.
2. Safety Warnings - Safety instructions / warning labels attached to the instrument • Safety notes on the instrument itself, which are marked with a warning triangle, indicate that the correct operating instructions (as defined in these Instructions for Use) must be followed when operating or replacing the item marked. Failure to adhere to these instructions may result in an accident, personal injury, damage to the instrument or accessory equipment.
2. Safety 2.3 Integrated safety devices The instrument is equipped with the following safety devices: • Knife guard (14) on the blade/knife holder. • Knife sledge locking knob (23) for the knife sledge. Knife sledge locked 14 Knife sledge is movable 23 11 24 Knife guard on the blade holder Fig. 2 Knife sledge locking knob The knife sledge is locked in place using the locking knob (23) that engages in the notch points on the bar (24), thus holding the knife sledge securely.
3. Instrument Components and Specifications 3.
3. Instrument components and specifications 3.2 Technical data General Approvals: The instrument-specific marks are located on the name plate. Operating temperature range: 10 °C to +40 °C Relative humidity: max.
3. Instrument Components and Specifications 3.3 Instrument specifications • The Leica SM2010 R is a manually operated sliding microtome, designed as a low-maintenance tabletop instrument with roller-guided knife sledges and automatic section thickness feed. • Stable, torsion-free basic design with micrometer feed system in a closed housing, protected from ingress of paraffin waste. • The vertical cross roller bearings have a cover which provides reliable protection from ingress of section waste.
4. Instrument Setup 4.1 Standard delivery The Leica SM2010 R standard delivery includes: 1 Leica SM2010 R basic instrument............................................... 14 0508 42258 1 section waste tray......................................................................... 14 0508 42328 1 tool set consisting of..................................................................... 14 0508 42983 1 Allen key size 6...............................................................................
4. Instrument Setup 4.3 Unpacking First check the shipment for external damages upon arrival. If it is evident that the shipment was damaged during transport, please make a claim to the carrier immediately. • • • 4.4 Open the packaging. Remove all foam material. Take out all accessories and the instruction manual. Setup Do not transport the instrument by holding it by moving parts, on the knife sledge, coarse driving wheel or the knob for setting the section thickness. Fig.
4. Instrument Setup Horizontal alignment For safe and accurate work, it is important that all instrument feet are in uniform contact with the installation surfaces. The microtome is horizontally aligned at the factory. If a completely level or horizontal surface is not available at the installation site, the instrument must be realigned. To do so, the two instrument feet (25) on the right side of the instrument are height-adjustable.
4. Instrument Setup 4.5 Assembling the knife holder SN 62 64.2 64.1 Installing the intermediate plate 65 • Lock the knife sledge (11) in place using the locking knob (23) so that it cannot be moved. • The intermediate plate (60) intended for the knife holder SN, is mounted on the mounting table (62) of the knife sledge (11). 60 23 11 Important! The mounting table must be absolutely clean and dry. There must not be any foreign particles on it. Otherwise, sections could become contaminated. Fig.
4. Instrument Setup Assembling the knife holder SN (continued) Fastening the knife holder 66 The knife holder SN (67) has two bores (64) and thus two different mounting positions for different sectioning requirements. 67 64.1 66.1 64.2 60 Fig. 11 • Press the clamping lever (66) of the knife holder (67) downwards and screw the bottom of the thread (66.1) into one of the two bores (64.1) or (64.2, Fig. 11) of the intermediate plate (60).
4. Instrument Setup Assembling the knife holder SN (continued) Enlarged detail: Scale for setting the clearance angle. 73 71 70 Setting the clearance angle (Fig. 13) • Unscrew the knurled head screw (69). • Set the required angle with the adjusting lever (70) using the scale for the clearance angle (71). The upper edge (73, red arrow in Fig. 13) of the lever (70) has to align with the index mark of the selected degree value. • To fix the setting in place, retighten the knurled screw (69).
4. Instrument Setup 4.6 Assembling the blade holder SE 54 65 62 50 23 11 The blade holder SE is designed for conventional disposable blades from all common manufacturers. It is available in two models: one for low-profile blades and one for high-profile blades. The blade holder SE has a lateral movement, so that the entire width of the blade can be used. Mounting the grooved plate • Lock the knife sledge (11) in place using the locking knob (23) so that it cannot be moved.
4. Instrument Setup Assembling the blade holder SE (continued) Inserting the blade holder SE 18 9 53 54 2. 1. 56 3. The stopper (18) serves for closing the insertion opening (56) for the T-piece in the grooved plate, in order that no section waste can accumulate there. Fig. 17 • Mount the blade holder (9) on the grooved plate (50) such that the T-piece (53) on the underside is inserted in the groove (54).
4. Instrument Setup Assembling the blade holder SE (continued) 52 55.1 55.2 Inclination (declination) of the blade holder SE • Unscrew the Allen screw (52) at the back of the blade-holder base using an Allen key SW 6. • Turn the blade holder to the required position. • Set the required declination (inclination of the blade holder to the cutting direction) on the scale (55.1) at the back of the blade holder (67). • On the grooved plate (60) there is an index mark (55.
4. Instrument Setup Assembling the blade holder SE (continued) Adjusting the clearance angle and declination 20 52 • The index marks (0° to 10°) for adjusting the clearance angle (43.1) are on the right side of the segment arc (13). • There is also an index mark (17) on the right side of the knife holder basis (43.2) which serves as a reference point when adjusting the clearance angle. • Loosen the screw (15) using a size 4 Allen key until the segment arc (13) can be moved.
4. Instrument Setup 4.6 Inserting the universal cassette clamp The object orientation allows for simple position correction of the specimen surface when the specimen is clamped into place. 40 You can use the quick clamping system (29) to hold all the available accessory specimen clamps (for more information, see Chapter 7 "Optional Accessories"). 36 To do so, proceed as follows: 29 • Move the object head (40) to the lower end position by turning the coarse driving wheel (36).
5. Operation 5.1 Operating elements and their functions 5.1.1 Section thickness setting The section thickness is set by turning the adjusting knob (33) on the left side of the microtome. The scaled knob has a notch for each value that can be set. Setting range: 0.5 - 60 µm from 0.5 - 5.0 µm in 0.5 µm increments from 5.0 - 10.0 µm in 1.0 µm increments from 10.0 - 20.0 µm in 2.0 µm increments from 20.0 - 60.0 µm in 5.
5. Operation 5.1.3 Manual feed 33 The lever (35) for the manual feed is at the front right of the instrument. • Each time the lever is pushed or pulled, this causes a feed motion for trimming or sectioning by the value set on the scaled adjusting knob (33). 35 5.1.4 Automatic feed The position of the adjusting knob (22) determines the point of the knife sledge movement where the automatic feed takes place. It should take place immediately in front of the specimen. Fig.
5. Operation 5.1.5 Directional fixture for specimen clamps All object clamps available as optional accessories can be inserted into the quick clamping device (29) of the directional specimen holder fixture, all object clamps available as optional accessories can be used. The object orientation allows for simple position correction of the specimen surface when the specimen is clamped into place. 29 Orienting the specimen 31 30 34 32 Fig.
5. Operation 5.2 Clamping the specimen in the universal cassette clamp (UCC) 45 Caution! Always clamp the specimen block BEFORE clamping the knife. Lock the knife sledge and cover the knife edge / blade edge with the knife guard prior to any manipulation of knife/ blade or specimen, prior to changing the specimen block and during all work breaks! 14 41 44 14 23 45 Fig. 27 24 • Move the cassette clamp (41) to the very bottom position by turning the coarse driving wheel.
5. Operation 5.3 4 Clamping a disposable blade 57 46 48 Be very careful when handling microtome knives or blades. The cutting edge is extremely sharp and can cause serious injuries! The blade holder has to be installed in the instrument before a blade is inserted! 14 6 Insert the blade (Fig. 28) 49 51 47 Fig. 28 46 • Push the knife guard (14) towards the right and push the lever (46) upwards to release the clamp of the pressure plate (48). • Flap the insertion aid (57) downwards.
5. Operation 5.4 Replacing the pressure plate 46 21 48 57 The blade holder SE can be converted from use of narrow blades to use of wide blades, and vice versa. To do this, both the pressure plate (48) and the matching insertion aid (57) have to be replaced. To replace them, proceed as follows: • Push the knife guard (14) towards the right and push the lever (46) upwards to release the clamp of the pressure plate (48). 14 Fig. 30 • Now carefully pull the insertion aid (57) out to the left (Fig.
5. Operation 5.5 Inserting the knife 66 69 23 67 72 Be very careful when handling microtome knives or blades. The cutting edge is extremely sharp and can cause serious injuries! The blade holder has to be installed in the instrument before a blade is inserted! 11 Fig. 33 72 67 • Lock the knife sledge (11) in place using the locking knob (23). • Please make sure that the knife holder is firmly clamped using the clamping lever (66) and that the knurled head screw (69) is tightened.
5. Operation 5.6 Sectioning Cutting into the specimen (trimming) For trimming, the specimen feed can be disengaged either by turning the coarse driving wheel (36) or by operating the manual feed lever (35). 14 • Hold the knife sledge (11) at the grip (12) and place the sledge behind the specimen. • Pull the knife guard (14) of the blade/knife holder to the right.
5. Operation 5.7 Changing the specimen or interrupting sectioning Lock the knife sledge and cover the knife edge with the knife guard prior to any manipulation of knife or specimen head, as well as prior to changing the specimen block and during all work breaks! • Lock the knife sledge and move the specimen clamp far enough downward that the new specimen fits below the knife/blade. • Cover the cutting edge with the knife guard.
6. Cleaning and maintenance 6.1 Cleaning the instrument • Always remove the knife or blade before detaching the knife/ blade holder from the instrument! • Always put the knives back into the knife case when not in use! • Never place a knife anywhere with the cutting edge facing upwards and never try to catch a falling knife! • When using cleaning agents, observe the manufacturer's safety instructions and the laboratory regulations valid in the country of use.
6. Cleaning and Maintenance Clean the instrument and outer surfaces • If necessary, the varnished outside surfaces can be cleaned with a mild, commercial household cleaner or soapy water and then wiped with a moist cloth. • To remove paraffin residue, xylene substitutes, e.g. Roth Histol (Roth, Karlsruhe), Tissue Clear (Medite), Histo Solve (Shandon), paraffin oil or paraffin removers, e.g. Paragard (Polysciences) can be used.
7. Optional accessories 7.1 Ordering information Supermega cassette clamp with adapter, silver....................................................................... 14 0508 42634 Universal cassette clamp with adapter, silver.......................................................................... 14 0508 42635 HN40 clamp with adapter, silver.................................................................................................. 14 0508 42637 Standard specimen clamp with adapter, silver......
7. Optional Accessories Supermega cassette clamp, with adapter, silver for mounting in the quick change system for specimen imaging Maximum specimen size: 75 x 52x35 mm (L x W x H) Order No.............................................. 14 0508 42634 Fig. 36 Universal cassette clamp (UCC), with adapter, silver for mounting in the quick change system for specimen imaging Maximum specimen size: 40 x 29 mm (L x W) Order No. ��������������������������������������������14 0508 42635 Fig.
7. Optional accessories Standard specimen clamp, with adapter, silver for mounting in the quick change system for specimen imaging Maximum specimen size: 79 x 60 mm (L x W) Order No.............................................. 14 0508 42632 Fig. 39 Dry ice tub with adapter, silver Order No.............................................. 14 0508 42641 1 pair of cold protection gloves, size 8 Order No.............................................. 14 0340 45631 Fig. 40 Dust cover Order No. ................
7. Optional Accessories Blade holder SE, assembly Easily converted from a low-profile to high-profile blade holder by switching the pressure plate. Adjusting the clearance angle using an Allen key. Blade holder declination with scale for reproducible adjustment up to 45°. Safe insertion of the disposable blade using a magnet and insertion aid on the blade holder. Central disposable blade clamp. Precise and safe, lateral displacement of the blade for using the entire blade length.
7. Optional accessories Knife holder SN, assembly for resharpenable knives or blade rails. Blade holder declination with scale for reproducible adjustment up to 45°. 2 clamping screws for quickly and securely clamping the cutting tool. Safe lateral displacement of the cutting tool for using the entire blade edge. Space-saving, integrated knife guard in signal color. Standard delivery: - 1 intermediate plate..................... 14 0508 44671 - 5 cheese-head screws................
7. Optional Accessories Plastic knife guard, for blade rails Order No.............................................. 14 0368 33772 Fig. 47 Fig. 48 Knife, 16 cm Profile C, steel flat on both sides, for wax and frozen sections. Note: including knife case 14 0213 11140 Order No. ............................................ 14 0216 07100 Knife, 16 cm Profile D, steel Note: including knife case 14 0213 11140 Order No. ............................................ 14 0216 07132 Fig. 49 Fig.
7. Optional accessories Leica low-profile disposable blades - Type 819 Length 80 mm, height 8 mm 1 package of 50 pieces Order No.............................................. 14 0358 38925 Fig. 52 Leica high-profile disposable blades - Type 818 Length 80 mm, height 14 mm 1 package of 50 pieces Order No.............................................. 14 0358 38926 Fig. 53 38 Instructions for Use V 1.
8. Troubleshooting In the following table there is a list of the most common problems which can arise while working with the instrument, along with possible causes and troubleshooting procedures. Problem Possible cause Corrective action 8.1 Possible faults • The blade is not clamped properly. • Reclamp the blade. • Blunt blade/knife. • Laterally displace the blade/knife holder or insert a new blade/knife. 1. Thick/thin sections The sections alternate between being thick and thin.
9. Warranty and Service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or characteristics warranted. The scope of the warranty is based on the content of the concluded agreement.
10.
Decontamination Certificate (Master) Decontamination Certificate 11. Dear Customer, Any product that is to be returned to Leica Biosystems or serviced on site, must be cleaned and decontaminated in the appropriate manner. Since it is not possible to decontaminate for prion diseases, such as CJD, BSE, CWD etc., equipment exposed to specimens containing prion diseases cannot be returned to Leica Biosystems for repair.
** Such equipment must not be returned without the written agreement of Leica Biosystems. Yes No 11. Decontamination Certificate (Master) equipment has been prepared to ensure safe handling/transportation. 3 The Whenever possible, please use the original transportation case/box. Important - to avoid refusal of shipment: Place one copy in the unit prior to packaging, or hand it over to the service engineer.
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