Living up to Life User Manual Leica M60 B Leica M80 B
Copyright All rights to this documentation are held by Leica Microsystems CMS GmbH. Reproduction of text or illustrations (in whole or in part) by print, photocopy, microfilm or other method (including electronic systems) is not allowed without express written permission from Leica Microsystems CMS GmbH. The instructions contained in the following documentation reflect state-of-the-art technology. We have compiled the texts and illustrations as accurately as possible.
Function of the Microscopes The Leica M60 B and Leica M80 B microscopes, for which this User Manual has been written, are designed for routine examinations of cell and tissue cultures, liquids and sediments. This includes examining specimens taken from the human body for the purpose of gaining information about physiological or pathological conditions or inborn anomalies, or testing for safety and compatibility for potential recipients, or for monitoring therapeutic measures.
General Notes Use in clean rooms The Leica M series can be used in clean rooms without any problems. Cleaning ★★ Do not use any unsuitable cleaning agents, chemicals or techniques for cleaning. ★★ Never use chemicals to clean colored surfaces or accessories with rubberized parts. This could damage the surfaces, and products could be contaminated by abraded particles.
Important Safety Notes User Manual This User Manual describes the special functions of the individual modules of the Leica M stereomicroscopy series and contains important instructions for their operational safety, maintenance, and accessories. You can combine individual system articles with articles from external suppliers (e.g. cold light sources, etc.). Please read the User Manual and the safety instructions from the supplier.
Symbols Used Warning! Safety hazard! This symbol indicates especially important information that is mandatory to read and observe. • Danger due to hot surface. This symbol warns against touching hot surfaces, e.g. those of light bulbs. Failure to comply can cause the following: ★★ Hazards to personnel ★★ Functional disturbances or damaged instruments Important information This symbol indicates additional information or explanations that are intended to provide clarity.
Safety Instructions Description ★★ The individual modules fulfill the highest requirements for observation and documentation of Leica stereomicroscopes of the M series. Intended use of instrument ★★ Leica Microsystems microscopes are optical instruments for improving the visibility of objects or specimens through magnification. Accessories such as optical accessories, stands, illumination, cameras etc. supplement the equipment configuration.
Safety Instructions (continued) Responsibilities of person in charge of instrument ★★ These Safety Instructions must be available at the workplace. Ensure that: ★★ The M series of stereomicroscopes and accessories are operated, maintained and repaired by authorized and trained personnel only. ★★ All operators have read, understood and observe this User Manual, and particularly the safety regulations. Repairs, service work ★★ Repairs may only be carried out by Leica Microsystems-trained service technicians.
Safety Instructions (continued) Legal regulations ★★ Observe the generally applicable statutory and country-specific regulations for accident prevention and environmental protection. EC Declaration of Conformity ★★ Electrically operated accessories are constructed based on the state of the art of technology and are provided with an EC Declaration of Conformity. See page 54.
Safety Instructions (continued) Light sources: safety regulations ★★ Light sources pose a potential irradiation risk (glare, UV radiation, IR radiation). Therefore, lamps have to be operated in closed housings and in installed condition. ★★ Never look directly into the beam path (blinding hazard). ★★ Do not select a white, strongly reflective background for the specimen.
Contents Copyright 2 Function of the Microscopes 3 General Notes 4 Important Safety Notes 5 Symbols Used 6 Safety Instructions 7 Leica M Series Congratulations! 14 Maximum Compatibility 15 On We Go 16 Assembly Base and Focusing Column 18 Microscope Carrier 19 Optics Carrier 20 Tube 21 Eyepieces 22 Objective 23 TL ST Transmitted Light Base (for Leica M60 B) and TL5000 Ergo Transmitted Light Base (for Leica M80 B) 24 Leica M series Quick Start Guide The Reliable Route to Success 26 Overview of a
Bases Leica TL ST Transmitted Light Base: Controls (Leica M60 B) Leica TL ST Transmitted Light Base: Operation Leica TL ST Transmitted Light Base: Lamp Replacement Leica TL5000 Ergo Transmitted Light Base: Controls (Leica M80 B) Leica TL5000 Ergo Transmitted Light Base: Operation 43 44 45 46 47 Specifications for the Bases Leica TL ST Transmitted Light Base Leica TL5000 Ergo Transmitted Light Base 50 51 Appendix Calculating the Total Magnification and Field of View Diameter Care, Maintenance, Co
Leica M Series Leica M series User Manual 13
Congratulations! The New Leica M Series Leica Microsystems introduces the Leica M60 B and M80 B, two new highquality routine stereomicroscopes of the CMO product line from Leica Microsystems. The optical brilliance and wide range of accessories make them the ideal stereomicroscopes for quality assurance and for in-vitro diagnostics examinations.
Maximum Compatibility Leica engineers were careful to ensure that the new Leica M series – like its predecessors – remains compatible with existing series. This means that objectives, bases, tubes and so on can be reused. Objectives All objectives of this new generation have the same reference focal length as the objectives of the MZ generation. If you prefer, you can therefore continue to use the previous Leica objective series.
On We Go If your new Leica microscope has already been assembled and commissioned by your Leica consultant, click here to skip through the installation instructions and go directly to the Quick Start Guide on page 25 . Leica M series If, on the other hand, you are assembling your Leica microscope yourself, continue with the "Assembly" chapter, which begins on page 17.
Assembly Leica M series User Manual 17
Base and Focusing Column The first step is to connect the focusing column of the M series to the corresponding base. Assembling the column adapter 1. Remove the three screws provided from the column. 2. Install the base on the column using the three screws. Tools used ★★ Allen key, 3 mm Tip: Place the column on the edge of a table to attach the base and have another person help you.
Microscope Carrier Tool ★★ Allen key provided Alternative installation options Depending on the desired working distance, the microscope carrier can be installed in the standard way or upside down (see figure below). Assembly The microscope carrier is fastened to the column using the screw provided.
Optics Carrier Tools used ★★ None Assembling the Optics Carrier 1. Unscrew the clamping screw on the holder for the optics carrier. 2. Tighten the clamping screw below the optics carrier. 2. Place the optics carrier into the holder and align it in such a way that the notch in the optics carrier and the bottom clamping screw are on top of each other.
Tube All intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner, as is the IC80 HD camera module (for the Leica M80 B). Preparations 1. Unscrew the positioning screw and remove the protective cover. Refer also to the separately provided User Manual for the IC80 HD camera module. 3. While holding the tube only slightly, carefully tighten the positioning screw. It is automatically brought to the correct position. • may not be used for diagnosis.
Eyepieces Tools used ★★ No tools required. Magnification range You can extend the overall magnification range using available 10×, 16×, 25× and 40× widefield eyepieces for eyeglass wearers. Preparation 1. If you want to use an optional graticule, insert it now (page 39). Inserting the eyepieces 3. Push the eyepieces into the tubes as far as they will go and check to ensure that they fit tightly and accurately. 2. Remove the plastic tube guard. 4. Securely tighten the clamping screws.
Objective Tools used ★★ No tools required. Preparation 1. Remove the protective cap on the optics carrier by turning it. Attaching the objective Hold the objective firmly during assembly and disassembly so that it does not fall onto the stage plate. This applies particularly to the 2x planapochromatic objective, which is very heavy. Remove all specimens from the stage plate first. • 2. Screw the objective clockwise into the optics carrier.
TL ST Transmitted Light Base (for Leica M60 B) and TL5000 Ergo Transmitted Light Base (for Leica M80 B) Unpacking the base The base is delivered with the adapter plate installed. Make sure the instruments are unpacked on a flat, sufficiently dimensioned, and non-slip surface. Focusing drive and column 1. Unscrew the extension plate from the base using the Allen key provided. 2. Attach your focusing drive column to the bottom using the 4 Allen screws. 3.
Quick Start Guide Leica M series User Manual 25
The Reliable Route to Success Your Leica stereomicroscope has been delivered in completely assembled condition by your Leica partner, and naturally you want to get right to work. This manual will familiarize you with the finer details of your microscope. The following pages contain important, practical information that makes using it every day easier.
Overview of an M Series Microscope 1 2 1 Eyepieces for eyeglass wearers with dioptric correction and eyecups 8 9 3 4 10 5 6 2 Fastening screws for the eyepieces 3 Ergo tube 4 Magnification changer (zoom) 5 Optics carrier 6 Interchangeable objective 7 Transmitted light base 8 Column 10 Coarse/fine focusing 7 Leica M series User Manual 27
The Correct Interpupillary Distance The interpupillary distance is correctly set if you see a single circular image field when looking at a specimen. If you are still a novice microscope user, you may need a short time to become accustomed to this. Not to worry – after a little while, it will become automatic. Adjusting the interpupillary distance 1. Look into the eyepieces. 3. Slowly approach the eyepieces with your eyes until you can see the complete image field without corner cutting. 2.
Using the Eyepieces The eyepieces form the connection between the tube and the eye of the observer. Simply push them into the tube and they are ready to use. If you do not wear glasses: 1. Hold the eyepiece firmly and rotate the eyecups forwards counterclockwise. 2. If an eyepiece is equipped with the inte- If you wear glasses: 1. Hold the eyepiece firmly and rotate the eyecups counterclockwise towards the rear, as otherwise the viewing distance is too great.
Focusing Focusing raises or lowers the stereomicroscope using the focusing drive. The specimen detail is brought into sharp focus as soon as it is in the focal point of the objective. ★★ The focusing drive can be operated either left- or right-handed. • load of up to 15 kg. The coarse/fine adjustment carries a The resolution of the coarse/fine adjustment is 1 µm.
Adjusting the Resistance of the Focus Drive Adjusting the resistance Is the focusing drive too loose or too tight? Does the equipment tend to slide downwards? The resistance can be adjusted individually depending on the equipment weight and personal preferences as follows: 1. Grip the outer drive knobs with both hands and turn them towards each other until the desired resistance is reached during focusing.
Changing the Magnification (Zoom) All M series microscopes have an integrated zoom. The name indicates the zoom range covered: Zooming 1. Look into the eyepieces. 2. Focus on the specimen. ★★ Leica M60 B = Zoom 6:1 ★★ Leica M80 B = Zoom 8:1 3. Rotate the magnification changer until the desired magnification is configured. The rotary knob for the zoom can be used either left or right-handed.
Click Stops and Magnification Levels The zoom button can optionally be operated either with or without click stops. Continuous zoom is possible when the click stops are disabled, which many users find convenient. On the other hand, when the click stops are enabled, photographs, measurement results etc. can be reproduced more accurately. Leica M series Enabling and disabling click stops 1. Push the button towards the knob to enable the click stops. 2.
Eyepieces Leica M series User Manual 34
Magnification Factor of the Eyepieces An eyepiece not only makes it possible to look passively into the microscope, but also has a critical effect on the maximum magnification. The magnification factor is between 10× and 40×.
Health Notes Potential sources of infection Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the eye. The risk can be kept to a minimum by using individual eyepieces or detachable eyecups. Eyecups can be ordered separately. Please contact your Leica partner. • Separate eyecups are an effective way of preventing infections.
Dioptric Correction All Leica eyepieces are also available with builtin dioptric correction, allowing the microscope to be used without glasses even by those with vision problems. The correction comprises ±5 diopter settings. Using the dioptric correction 1. Set the dioptric correction of both eyepieces to the mid position ("0" diopter settings). 2. While wearing your glasses, look through the eyepieces and focus on the specimen. 3. Rotate both eyepieces to the maximum value of "+5". 4.
Dioptric Correction and Parfocality Leica stereomicroscopes are parfocally matched. The prerequisite for this is the correct setting of the diopters and the parfocality. The following adjustments have to be carried out only once by each user. Adjusting 1. Set the dioptric correction for both eyepieces to "0". Preparations ★★ Move the lever of the video/phototube to the "observation" position and open the diaphragm. 3. Select the highest magnification and readjust the sharpness. 2.
Graticules Use Leica graticules make counting easier, particularly for workstations that are not equipped with a digital camera. 2. Clamp the graticule on the insert, applying moderate pressure. Ensure that the graticule fits tightly. the eyepiece in the tube and then tightening it using the clamping screw. The Leica graticules for length measurements and numbering are fitted in mounts and are inserted into the eyepieces. 1. Screw the insert off of the eyepiece. 3.
Objectives and Optical Accessories Leica M series User Manual 40
The Different Types of Objectives To meet the various requirements regarding imaging properties, there is a choice of highquality interchangeable planachromatic and planapochromatic objectives and also lowerpriced interchangeable achromatic objectives. ★★ Achromatic objectives are particularly suited for three-dimensional specimens with high-contrast structures. ★★ Flat-field (planachromatic) objectives are particularly well suited for studying flat objects such as wafers and thin sections.
Bases Leica M series User Manual 42
Leica TL ST Transmitted Light Base: Controls (Leica M60 B) Refer also to the separately provided User Manual for the TL ST transmitted light base.
Leica TL ST Transmitted Light Base: Operation Light intensity control The left control adjusts the intensity of the 12 V/20 W halogen illumination. 1. Switch on the illumination of the base at the power switch. Control of the transmitted light The TL ST transmitted light base has a slider that automatically moves the path-folding mirror in the base when moved. The mirror is kept in the correct position at all times and permits smooth changeover between bright field and opaque transmitted light.
Leica TL ST Transmitted Light Base: Lamp Replacement Changing the halogen lamp Before you change the lamp, it is absolutely necessary to unplug the power plug from the base to prevent the risk of electric shock! • Changing lamps 1. Unscrew the two screws on the cooling unit and pull the cooling unit out, along with the lamp. Safety precautions When inserting the lamp, ensure that the cables are inside the two metal clamps. This prevents the cables from getting caught during insertion.
Leica TL5000 Ergo Transmitted Light Base: Controls (Leica M80 B) 1 2 Rear side of the TL5000 Ergo 1 2 Power switch for the base Power supply connection 1 2 3 45 Left side of the TL5000 Ergo 1 2 3 4 Refer also to the separately provided User Manual for the TL5000 Ergo transmitted light base. Leica M series 5 LED display for opening and positioning the aperture or for balance in DF mode.
Leica TL5000 Ergo Transmitted Light Base: Operation The transmitted light base must only be connected to a grounded socket with a faultless power cable! Failure to observe these warnings may result in serious personal injury or even death! Switching the transmitted light base on and off 1. Switch on the transmitted light base with the power switch on the rear side. 2. Press the on/off switch on the right side once to turn on the illumination.
Leica TL5000 Ergo Transmitted Light Base: Operation (Continued) 3. Look through the eyepiece and adjust the light intensity using the control on the right side. The intensity of the illumination is visualized by the LED scale. 4 The following assumes that the power switch on the rear side of the device is always switched on. This switch is not mentioned in the remaining part of this User Manual. Press the on/off switch on the right side once again to turn off the illumination on the base.
Specifications for the Bases Leica M series User Manual 49
Leica TL ST Transmitted Light Base Light source Quick illuminant change Illuminated area Power supply Connections Weight Illumination modes Bright field Dark field Oblique light Relief Contrast System (RC™) CCIC (Constant Color Intensity Control) Internal shutter/lamp control Integrated filter holder Coated optics for increasing the color temperature Matching of high num.
Leica TL5000 Ergo Transmitted Light Base Light source Light source Illuminated field: Bright field ∅ Illuminated field: Dark field ∅ Relief Contrast System (RC™) Internal shutter/lamp control Integrated filter holder Matching of high num. aperture Remote control options AntiShock™ Pads Dimensions (W×H×D) Power supply Input Output Connections Power supply Leica M series LED 65 mm 40 mm Yes Yes Yes Yes Yes Yes 412×341×46 mm 100–240 VAC 47 – 63 Hz 3.2 A 33 VDC 3.
Appendix Leica M series User Manual 52
Calculating the Total Magnification and Field of View Diameter Parameter NFOV Field number of the eyepiece. Field numbers are printed on the eyepieces: 10× = 21, 16× = 14, 25× = 9.5, 40× = 6. MO Magnification of objective ME Magnification of eyepiece z Magnification changer position q Tube factor, e.g. 1.5× for coaxial incident light, 1.6× for 45° ErgoTube™ MO 1× objective ME 25×/9.5 eyepiece Factor 1.
Care, Maintenance, Contact Persons We hope you enjoy using your high-performance microscope. Leica microscopes are renowned for their robustness and long service life. Observing the following care and cleaning tips will ensure that even after years and decades, your Leica microscope will continue to work as well as it did on the very first day.
Care, Maintenance, Contact Persons (continued) Protection from dirt Dust and dirt will affect the quality of your results. ★★ Put a dust cover over the microscope when it will not be used for a long time. Cleaning polymer components Some components are made of polymer or are polymer-coated. They are, therefore, pleasant and convenient to handle. The use of unsuitable cleaning agents and techniques can damage polymers.
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