Instructions for Use Leica CM1950 Cryostat Leica CM1950, V 1.5 RevF, English – 08/2014 Order No.: 14 0477 80101 RevF Always keep these instructions near the instrument! Read carefully before working with the instrument.
1. NOTE The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state-of-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.
Table of Contents 1. Important Information... ������������������������������������������������������������������������������������������������������������ 6 2. Safety and Design... ������������������������������������������������������������������������������������������������������������������� 8 3. 4. 5. Technical Data... ���������������������������������������������������������������������������������������������������������������������� 16 Standard delivery...
Table of Contents 7. Instrument Controls... �������������������������������������������������������������������������������������������������������������� 33 8. Working with the Instrument... ���������������������������������������������������������������������������������������������� 39 9. Troubleshooting... �������������������������������������������������������������������������������������������������������������������� 51 10. 11. Temperature selection chart (- °C)...
1. Important Information 1.1 Symbols in the text and their meanings Warnings appear in a gray box and are marked by a warning triangle . Useful tips, i.e. important user information, appear in a gray box and are marked by an . Caution – UVC radiation! (5) (Fig. 5) Numbers in parentheses refer to item numbers in illustrations or to the illustrations themselves. This product fulfills the requirements of the Council's Directive 98/79/EC concerning in vitro diagnostics (IVD) medical devices.
1. Important Information 1.2 Instrument type 1.3 All information given in these Instructions for Use applies only to the instrument type indicated on the title page. A nameplate indicating the instrument serial number is attached to the rear side of the instrument (Fig. 1 is provided as an example only and shows a valid nameplate for this instrument with the necessary information about instrument type and power requirement. Precise data for the various versions is specified in Chap. 3 "Technical Data".
2. Safety and Design The safety and caution notes in this chapter must be observed at all times. Be sure to read these notes even if you are already familiar with the operation and use of other Leica products. 2.1 Safety notes These Instructions for Use include important instructions and information related to the operating safety and maintenance of the instrument.
2. Safety and Design The instrument has been designed and constructed with the latest state-of-the-art technology and according to recognized standards and regulations with regard to safety technology. Operating or handling the instrument incorrectly can place the user or other personnel at risk of injury or can cause damage to the instrument or other property. The instrument may be used only as intended and only if all of its safety features are in proper working condition.
2. Safety and Design When the instrument is delivered, check the tilt indicators on the packaging. If the arrowhead is blue, the shipment was transported laying flat, was tilted at too great an angle or fell over during transport. Note this on the shipping documents and check the shipment for possible damage. Fig.
2. Safety and Design 9 10, Transport grip points Fig. 6 7 8 Fig. 5 • Lift and remove the wooden edge (7, Fig. 5). • Remove the ramp (6, Fig. 4) from the pallet. • Properly insert the ramp. Make sure that the ramp components designated "L" (left) and "R" (right) click into place in the intended guide channel (10, Fig. 6a). When correctly assembled, the guide rails (11) are located inside, while the arrows (12) point toward each other. 6 6 10 12 11 Fig.
2. Safety and Design 2.5 Safety devices The Instructions for Use include important instructions and information related to the operating safety and maintenance of the instrument. These Instructions for Use are an important part of the product, and must be read carefully before startup and use and must always be kept near the instrument.
2. Safety and Design Knife guard Prior to making modifications to the knife and specimen, changing the specimen or knife, or taking a break, always lock the handwheel and cover the cutting edge with the knife guard! The CE, CN and CN-Z knife holders feature knife guards; the glass anti-roll plate of the CE knife holder also serves as a knife guard. 2.6 Securing/locking the handwheel 12 o'clock position 6 o'clock position Fig.
2. Safety and Design 2.7 Cleaning, disinfection – Turning the instrument back on It is not necessary to remove the microtome for disinfection. • The instrument has been designed for UV disinfection! Spray disinfection with Leica Cryofect is also possible, thanks to the special insulation of the microtome. (Cryofect is not available in all countries!) Remove section waste after EVERY sectioning operation and BEFORE changing specimens.
2. Safety and Design 2.
3. Technical Data All specifications related to temperature are valid only up to an ambient temperature of 18 °C to 35 °C and a relative humidity of no more than 60 %! Instrument type -1 -2 -3 Nominal voltage (±10 %) Nominal frequency Power draw Max. start-up current for 5 sec. Protective class Automatic cutout Pollution degree Overvoltage category Heat emission (max.) Approval 100 VAC 50/60 Hz 1900 VA 35 A eff. I T15A M3 2 II 1900 J/s CE 120 VAC 60 Hz 1900 VA 35 A eff.
3. Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 Hz 0 °C to -35 °C ± 5 K, adjustable in 1 K increments, at an ambient temperature of 20 °C approx. 5 h approx. 8 h 690 W 25 bar 3 300 g (± 5 g), refrigerant R-404A* 0.6 l EMKARATE RL22S, ICI* 0 °C to -35 °C ± 5 K, adjustable in 1 K increments Ambient temperature of 20 °C approx. 5 h approx. 8 h 690 W 25 bar 3 300 g (± 5 g), refrigerant R-404A* 0.
4. Standard delivery Basic instrument WITHOUT motor/WITHOUT extraction, in the specific voltage variant 1 handwheel, manual ................................................................................... 14 0477 41346 5 specimen discs, 3 mm................................................................................ 14 0477 40044 1 section waste tray...................................................................................... 14 0477 40062 1 position holder for freeze shelf................
4. Standard Delivery Basic instrument WITH motor/WITHOUT extraction, in the specific voltage variant 1 handwheel, motorized............................................................................... 14 0477 41347 5 specimen discs, 30 mm.............................................................................. 14 0477 40044 1 section waste tray...................................................................................... 14 0477 40062 1 position holder for freeze shelf......................
5. General Overview Emergency-stop switch (motorized instruments only) On/off switch, also circuit breaker Footswitch dummy (motorized instruments only) Cryostat chamber (with activated UV disinfection) Handwheel in 12 o'clock position Bacteria filter (optional, only for instruments with filter) Condenser Catch tank for condensate Unscrew and align adjusting feet after transport Fig.
5. General Overview 5.1 1 2 3 Control panel fields and cryostat chamber Control panel field 1: Extraction, temperature and time control, illumination, UV disinfection Control panel field 2: Electric coarse feed (sectioning and trimming thickness adjustment) Control panel field 3: Motorized sectioning, optional (adjustment of stroke type, cutting speed etc.) 1 7 15 10a 5 10 11 10b 2 16 10c 6 4 14 3 8 13 9 12 Fig.
6. Installation 6.1 Site requirements Do not operate the instrument in rooms with explosion hazard. The place of installation must meet the following requirements: - The instrument requires an installation area of approx. 835 x 850 mm, - Room volume must be at least 8 m3, - Room temperature consistently 18 °C to 35 °C, - Temperature range during storage: 5 °C to 55 °C, - Relative humidity, maximum 60 % (non-condensing) - Storage humidity: < 60 % - Elevation: up to a max.
6. Installation 6.2 Transport to the site • First, check if the location meets the conditions specified in "Site requirements". • Transport the instrument to the desired location. • Observe the following: The instrument must be transported in an upright position or slightly tilted (max.
6. Installation Transport with a fork lift • The instrument can be transported with a fork lift. To ensure a safe transport with a fork lift 3 people are required: one operating the fork lift, and the other 2 holding the instrument on either side to prevent it from sliding down. • At the installation location, unscrew the adjusting feet (1) (see Fig. 12) using the open-end wrench (13 mm). This is absolutely necessary for the instrument to stand stably. Fig. 13, Total width with handwheel 6.
6. Installation 6.3.1 Locking/unlocking the handwheel 12 o'clock position 6 o'clock position Fig. 15 1 2 Rotate the handwheel only if the refrigeration system is on and the cryochamber is cold. Always lock the handwheel prior to making modifications to the knife or specimen, changing the specimen, or taking a break! To lock the handwheel, move its handle to the 12 or 6 o'clock position.
6. Installation 6.4 Electrical connection After transporting, wait at least 4 hours before turning the instrument on. This waiting period is necessary to allow the compressor oil, which may have been displaced during transport, to return into its original position. Furthermore, any condensation that has formed during this time due to temperature fluctuations must be allowed to dry completely.
6. Installation 6.5 Installing accessories/inserting chamber accessories 6.5.1 Installing the adjustable footrest (optional) • To install the optional footrest, the screws (1) must be unscrewed using the size 3 Allen key provided. When installing the holder (2), ensure that the cutout faces downwards so that the support (3) can be hooked in. 1 • Attach the holders (2) for the footrest on the left and right to the front wall of the housing from the outside.
6. Installation 6.5.2 Installing the storage systems (optional) 1 2 Fig. 21 For reasons of accessibility, the (optional) storage system must always be installed first. To do so, remove the insert (1), place the frame (2) in front of the bore and tighten the screws/ washer on the cryostat housing using the size 4 Allen key. Afterwards, insert the insert (1) into the frame and fold it up. 6.5.
6. Installation 6.5.5 Installing the heat extractor, stationary (optional) The holder (1, Fig. 24) of the heat extractor is screwed to the left housing wall using the size 4 Allen key provided (it is better to begin with the bottom screw). Then, rotate the holder upwards (see arrow) and insert and tighten the top screw. 1 Fig. 24 Now attach the cover for the quick freeze shelf to protect the shelf from frost. For temperature reasons, install the knife/blade holder on an appropriate base. 6.5.
6. Installation • Push the knife/blade holder base (2) on the dovetail guide (4) from the front and tighten it using the clamping lever (5). Move the clamping lever clockwise (toward the closed lock symbol) on the right side of the blade/knife holder base (see Detail of Fig. 26).
6. Installation 6.5.7 Inserting/changing the bacteria filter Fig. 27 The holder for the bacteria filter (optional) is visible in the front of the instrument. • To insert the filter, hold it with one hand, press on the right of the socket, then guide the filter into the tube from the left. 2 A L 3a 3 Fig. 28 E Fig. 29 Leica CM1950 – Cryostat The filter must be disposed of according to valid laboratory specifications.
6. Installation 6.5.9 Installing the section extraction (optional) – Use with blade holder CE only A C B D G H Fig. 30 • • • • • F E Silicone hose (A) with hose adapter 1 (B, for filter in instrument), hose adapter 2 (C, for suction nozzle D or F) and suction nozzle (D) – factory pre-assembled Silicone stopper (E) Chamber suction nozzle (F) Filter (G) Plastic clips (H), for parking the chamber suction nozzle. Mark for suction nozzle Fig.
7. Instrument Controls 7.1 Control panel fields on the CM1950 – Control panel field 1 Display of actual and target chamber temperatures Display of actual and target specimen head temperatures Display of real time, defrost time and error messages Fig.
7. Instrument Controls A B 12 o'clock Position 6 o'clock Fig. 35 • Press the "VAC" button to enable the vacuum extractor. The LED in the "VAC" button is lit while the extractor is on. Press the button again to disable it. • Use the knob to adjust the intensity of the vacuum.
7. Instrument Controls Control panel field 2 – Electric coarse feed, sectioning and trimming thickness display Illuminates in yellow when the specimen is in retraction. LED for trimming and section thickness display Toggle TRIM and SECT (LED active). Rocking mode – only in manual mode; in rear area, i.e. approx. 12 - 3 o'clock position of the handwheel (rock handwheel back and forth a short distance). Press approx. 3 sec.; "on" or "off" appears (for the retraction).
7. Instrument Controls Coarse feed functions The electric coarse feed at two speeds is used for a rapid movement of the specimen towards and away from the knife. With the double-arrow buttons, the coarse feed operates at 900 µm/s; with the single-arrow buttons, it runs at 300 µm/s. Retracting the specimen head from the knife • Pressing once starts the rapid backwards movement to the rear end 1 position (Home position). LED (1) flashes, while the specimen head is in motion.
7. Instrument Controls Control panel field 3 – Motorized sectioning (optional) Switching the sectioning mode ("CUT MODE") from continuous stroke ("CONT") to single stroke ("SINGLE") (active) If emergency stop has been activated, the cutting mode must be selected again. The red LED in the E-STOP field indicates either: • emergency-stop function active, or • Footswitch dummy (opt. foot switch) not connected or incorrectly connected.
7. Instrument Controls Disinfection Duration - 30 min Duration – 180 min Fig. 39 UVC key - to activate / deactivate the disinfection cycle and/or to acknowledge interruption of a disinfection cycle. To start disinfection, the sliding window must be completely closed. • Press UVC key once briefly to start the 30 min. mode • UVC button – press 1x for a longer time (approx. 4 sec), 180 min mode For current information about certificates and recommendations, visit: http://www.leicabiosystems.
8. Working with the Instrument 8.1 Preparing cutting tools, specimen discs and preparation aids The knives are extremely sharp! Handle with care! Never try to catch a falling knife! • Place working materials such as the blade box or knives (in the knife case), brush, forceps or preparation needles and, where applicable, specimen discs into the cryostat chamber.
8. Working with the Instrument 8.3 Configuring the parameters The instrument must be switched on at least 5 hours before the planned use. • Turn the lamp on or off. Fig. 42 • This button activates or deactivates the manual defrost of the cooling chamber, specimen head or freeze shelf. (For more detailed handling instructions, refer to the chapter on "Working with the Instrument" in these Instructions for Use.) • Pressing the key button for approx. 5 sec.
8. Working with the Instrument Programming the specimen temperature • Select the desired temperature of the specimen. • The specimen temperature is set and indicated on the control panel marked with the specimen head symbol. The actual temperature is the standard indication. Briefly pressing the "+" or "-" button displays the target temperature. Set the desired value via the "+" / "-" buttons. Pushing the "+" or "-" button for more than 1 sec. increases or decreases the specimen temperature continuously.
8. Fig. 46 Working with the Instrument Programming the defrost cycle • Set the beginning of the automatic defrost cycle. The automatic defrost cycle takes place once within 24 hours. It is set with the "+"/"-" buttons on the right of the panel with the clock symbol. The two buttons are marked by a melting snowflake . • Briefly press the "+" or "-" button for indication of the beginning of the defrost cycle which has actually been set.
8. Working with the Instrument Fig. 47 Fig. 48 Manual defrosting of the cryochamber • Push the manual defrost button (with the melting snowflake) on the left over the key button to activate the defrost cycle of the cryochamber on demand. • Activation is confirmed by an audible signal. • Then, push the "+" or "-" button on the panel for the cryochamber temperature. • The manual defrost cycle (12 min.) is activated.
8. Working with the Instrument Entering the trimming thickness To switch from a trimming section thickness for research applications (1- 600 µm) to a thickness for clinical applications (10, 20, 30 or 40 µm), press and hold down the TRIM/SECT key while switching on the instrument. Fig. 50 Fig. 51 • Press the TRIM/SECT button. TRIM mode is active if the LED at the top right is illuminated.
8. Working with the Instrument 8.4 Working with the precooled cryostat 8.4.1 Preparatory work • Lock the handwheel in the top (12 o'clock) position. • Cut the specimen to size outside of the cryostat. • Select the precooled specimen disc, cover it with freezing compound, then attach and orient the specimen. The safety gloves included in the standard scope of delivery must be worn when working inside the cryostat chamber! Fig.
8. Working with the Instrument Check the stability of the specimen head each time it clicks back into place. This can prevent it from becoming difficult to set the zero position. Before using them for the first time, degrease new knives using acetone or alcohol. • Approach the specimen with the knife or blade holder: • To do so, open the clamping lever of the base, approach the specimen and close the lever again. • Open the lever of the orientation.
8. Working with the Instrument K Ensure that the hose with the nozzle is not installed against its "natural" curvature on the pressure plate of the knife holder. B D I C The tension acting on the hose can be minimized by turning the red ring (Fig. 57, top right) clockwise so that the suction nozzle presses against the pressure plate (I, Fig. 57). A Fig. 57 • Check that the anti-roll guide is parallel and correctly adjusted. Readjust if necessary (see page 62). • Activate trimming mode.
8. Working with the Instrument If the handwheel is not moved for approx. 5 sec, the valves close and the fan remains on. If the handwheel is moved for approx. 1 min, the valves close, the fan shuts off (the LED in the VAC switch goes off to prevent icing). To continue working, you now have to reactivate the VAC button. Fig. 59 E Trimming with extraction – 2. Brush technique, finger rest installed • Remove the silicone stopper (E) from the filter cover (and keep it in a safe place).
8. Working with the Instrument 8.4.3 Cutting with extraction – Anti-roll guide installed • Shut off VAC (LED in VAC button goes out). • Switch from trimming to sectioning mode (important for section stretching, as the valves work differently than in trimming mode). • Set the desired section thickness. • Switch on the VAC and begin at level 1. If the section does not stretch correctly, turn the VAC knob higher in small increments.
8. Working with the Instrument After trimming or sectioning: Specimen: • Unclamp and thaw. • Immerse in fixative for further processing. Cleaning • Use the brush to sweep up the section waste (section waste tray) and remove it from the cryostat (follow applicable laboratory regulations for disposal).
9. Troubleshooting 9.1 Problems during work Problem Cause Remedy Frost on chamber walls and microtome Cryostat is exposed to air currents (open windows and doors, air conditioning). Frost built-up by breathing into the cryochamber. Move to a draft-free location. Sections smear Specimen not cold enough. Anti-roll plate not yet cold enough, thus warming the section. Select lower temperature. Wait until knife and/or anti-roll plate have reached chamber temperature.
9. Troubleshooting Problem Cause Remedy Ridged sections Cryostat is exposed to air currents (open windows and doors, air conditioning). Frost built-up by breathing into the cryochamber. Use different part of the knife. Replace anti-roll plate. Chatter during sectioning Specimen insufficiently frozen onto the specimen disc. Specimen disc not clamped tightly. Knife not clamped tightly enough. Specimen has been sectioned too thickly and has detached from disc. Very hard, inhomogenous specimen.
9. Troubleshooting Problem Cause Remedy Blunt knife Inappropriate section thickness. Incorrect clearance angle. Microtome not dried thoroughly enough. Dried specimen. Use different part of the knife. Select correct section thickness. Set correct angle. Dry microtome. Anti-roll plate is too warm or incorrectly positioned. Fat on the corner or edge of anti-roll plate. Anti-roll plate not correctly fixed. Rust on the knife. Cool down anti-roll plate, or reposition correctly.
9. Troubleshooting Problem Cause Remedy No or insufficient refrigeration of the cryochamber Cooling system or electronic drive defective. Call technical service. Sliding window collects condensation Air humidity and room temperature too Comply with the requirements for high. the installation site. No or insufficient refrigeration of the specimen Cooling system or electronic drive defective. Call technical service. Sections not properly flattened Lamp defective. Switch defective.
10.
11. Optional Accessories 11.1 Ordering information Specimen disc ø 20 mm assembly................................................. 14 0477 43739 Specimen disc ø 30 mm assembly................................................. 14 0477 40044 Specimen disc ø 40 mm assembly................................................. 14 0477 40045 Specimen disc ø 55 mm assembly................................................. 14 0477 40046 Specimen disc 80 x 50 mm assembly ...........................................
11. Optional Accessories Heat extractor, assembly................................................................ 14 0477 43126 Section waste tray........................................................................... 14 0477 40062 Brush shelf......................................................................................... 14 0477 43036 Storage system, assembly.............................................................. 14 0477 42618 Shelf, movable............................................
11. Optional Accessories Bladeholder CE with anti-roll guide (for low-profile, LP, and high-profile, HP) 1 4 6 4 7 12 3 8 2 9 • • • • • • • • • • 5 Fig. 63 With blade ejector (1) With knife guard (2) integrated lateral shift and stable base Clearance angle adjustment (5) using size 4 Allen key (see Detail Fig. left on the blade holder) – recommended angle between 2° and 5°.
11. Optional Accessories The anti-roll guide and pressure plate must be parallel to each other. 4 1 6 7 3 8 2 Fig. 65 5 Blade holder CE with finger rest (for LP + HP) Blade rest (straightedge) for low-profile blades The knife guard is folded away in the detail view. Knife guard folded away 7a Four possible settings 6a 1 2 Red elements on the blade and knife holders, such as the knife guard and ejector, are protective devices that may not be removed.
11. Optional Accessories Inserting/ejecting the blade into/from the blade holder CE 4 The safety gloves included in the standard scope of delivery must be worn when inserting the blade! 9 8 11 10 Blade ejector 1. Fold the anti-roll guide system (4) to the left – while doing so, hold the lever (11) (not the adjusting screw of the anti-roll guide) so that the height of the anti-roll guide remains unchanged. 2. Open the clamping lever (10) by turning it counterclockwise. 3.
11. Optional Accessories The safety gloves included in the standard scope of delivery must be worn when disposing of the blade! Once the blade has been removed from the blade holder, it is disposed of into the dispenser container (storage compartment in bottom, Fig. 68), or according to laboratory regulations. Fig. 68 Lateral shift for blade holder CE 8 Fig. 69 Fig.
11. Optional Accessories Adjusting the blade holder with anti-roll guide You can adjust the height of the anti-roll guide system using the knurled nut (1): • If you turn the nut counterclockwise, the antiroll guide system moves toward the blade. • If you turn the nut clockwise, the anti-roll guide system moves away from the blade. 1 If the anti-roll guide system is in the wrong position relative to the cutter, the following problems will result: I Blade holder CE with glass anti-roll guide Fig.
11. Optional Accessories Cleaning the blade holder CE 35 Daily cleaning The safety gloves included in the standard scope of delivery must be worn when cleaning the blade holder to prevent frostbite of the skin. 33 34 35 36 Fig. 71 1. Fold the anti-roll guide system (33) to the left while holding it down by the lever (34). 2. Unscrew the bolts (35) of the pressure plate. 3. Afterwards, the pressure plate (36) can be removed for cleaning (with alcohol or acetone).
11. Optional Accessories Knife holder CN with anti-roll guide – Moving the clamping jaws and inserting the knife The specimen disc 50x80 mm is suitable only for section thicknesses up to approx. 5 µm (due to the large specimen size). 1 3a 3a 2 not visible 3 4 4 5 6 Fig. 73 The large specimen disc (80x50 mm) should preferably be used with knife holder CN and the 16 cm C-profile steel knife. The clamping jaws are factory-installed in the knife holder with a clearance of 64 mm.
11. Optional Accessories 19 18 19 Knife guard/lateral movement for knife holder CN The knife guard (18) is fixed and integrated into the clamping jaws. The knife guard has handles (19) that allow it to be moved. The knife guard is adequate for knives up to 22 cm in length. Always cover exposed parts of the knife blade after sectioning. The anti-roll guide system can be moved sideways (only for the 84 mm variant). To better find the mid position, a groove (17) is provided in the shaft (16).
11. Optional Accessories 70 Anti-roll guide system Anti-roll plate (with glass stage plate) Available with various spacers: • 70 mm - 50 µm, for section thickness: < 4 µm • 70 mm - 100 µm, for section thickness: 5 µm - 50 µm • 70 mm - 150 µm, for section thickness: > 50 µm mm Fig. 77 The 50 µm and 100 µm anti-roll plates are included in the standard scope of delivery of the blade holder CE. Straightedge (blade rest) Insert for low-profile blades for blade holder CE (14 0477 43005) replacement Fig.
11. Optional Accessories A Replacement filter for extraction system, pack of 25, with coarse filter insert (A) 14 0477 44013 • Change when extraction power is impaired. • When using the cryostat frequently, check the filter bag daily and replace as required. Fig.
11. Optional Accessories • Specimen discs in various dimensions The specimen disc 50 x 80 mm is suitable only for section thicknesses up to approx. 5 µm (due to the large specimen size). 50x80 mm ø 55 ø 40 ø 20 mm ø 30 O-rings in various colors • for plate Ø 20 mm (red or blue), 10x each • for plate Ø 30 mm (red or blue), 10x each • for plate Ø 40 mm (red or blue), 10x each • for plate Ø 55 mm (red or blue), 10x each for labeling specimen discs using color Fig.
11. Optional Accessories Footswitch, for use with motorized instruments only. The foot switch can be used to control the motorized sectioning process. It also has a function that is similar to the emergency stop function. Fig. 86 Caution! All control panel functions and all buttons on the instrument remain active along with the foot switch. • Using the CUT MODE button, select the desired operating mode CONT or SINGLE on the control panel (Fig. 38, page 37).
11. Optional Accessories Footrest, individually height-adjustable footrest with 5 adjustment options. Fig. 87 Brush shelf, for use with blade holder CE Fig. 88 Storage system, movable for installation in the front part of the cryostat for cooled storage of preparation aids Fig. 89 70 Instructions for Use V 1.
12. Maintenance and Cleaning 12.1 General maintenance instructions The microtome is virtually maintenance-free. To ensure a smooth operation of the instrument over several years we recommend the following: • At least once a year, have the instrument inspected by a qualified service engineer authorized by Leica. • Enter into a service contract at the end of the warranty period. For more information, please contact your local Leica technical service center. • Clean the instrument daily.
12. Maintenance and Cleaning Emptying the condensate bottle Check the fill level of the condensate bottle (1) visible in the front panel of the instrument at regular intervals. • The bottle collects the condensate that accumulates during defrosting. 1 Fig. 90 Dispose of the contents of the bottle in accordance with laboratory regulations. Basically, we recommend UV disinfection (for application, see Chap. 7 "Instrument Controls" - Disinfection).
12. Maintenance and Cleaning 12.2 Changing fuses • In case of power supply faults, please contact an authorized Leica service technician immediately. Do not carry out any repairs on your own as this will invalidate the warranty. Repairs may only be carried out by qualified service engineers authorized by Leica. 12.3 Replacing the UVC lamp Turn the instrument off and disconnect the power plug before replacing the UVC lamp.
12. Maintenance and Cleaning 4 3 5 Fig. 92 6 7 8 Fig. 93 Fig. 94 Dispose of the UVC lamp separately! 74 Removal of the lamp The UVC lamp (3) is installed in front of the protection screen for chamber illumination (4). • Hold the lamp with both hands and carefully pull it out of the clips (5) with a slight forward movement. • Detach the metal ring (7) on the holder (6) in the direction of the arrow (8) and carefully pull the lamp out of the holder with your right hand (see Fig.
12. Maintenance and Cleaning 12.3.1 Replacing the fluorescent lamp Switch the instrument off and disconnect the power plug before replacing the fluorescent lamp! If the lamp is broken, it must be replaced by the technical service, as the replacement involves a high risk of injury. 1 • Slightly lift the sliding window (1) holding it by the grip (2) and pull it out to the front. Removal of the lamp 2 Fig. 95 • The lamp is mounted behind a glare shield and therefore not visible.
13. Decontamination Certificate (Master) Decontamination Certificate Dear Customer, Any product that is to be returned to Leica Biosystems or serviced on site, must be cleaned and decontaminated in the appropriate manner. Since it is not possible to decontaminate for prion diseases, such as CJD, BSE, CWD etc., equipment exposed to specimens containing prion diseases cannot be returned to Leica Biosystems for repair.
** Such equipment must not be returned without the written agreement of Leica Biosystems. Yes No 13. Decontamination Certificate (Master) equipment has been prepared to ensure safe handling/transportation. 3 The Whenever possible, please use the original transportation case/box. Important - to avoid refusal of shipment: Place one copy in the unit prior to packaging, or hand it over to the service engineer.
14. Warranty and service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted. The scope of the warranty is based on the content of the concluded agreement.
