SMART 2 ••••••••••••••••••••••••••••••••••••••••••••••• Colorimeter OPERATOR’S MANUAL V.2.
TABLE OF CONTENTS GENERAL INFORMATION Packaging & Delivery ······································································5 General Precautions ········································································5 Safety Precautions ···········································································5 Limits of Liability ·············································································5 Limited Warranty············································································
TABLE OF CONTENTS (cont.
GENERAL INFORMATION n PACKAGING & DELIVERY Experienced packaging personnel at LaMotte Company assure adequate protection against normal hazards encountered in transportation of shipments. After the product leaves the manufacturer, all responsibility for its safe delivery is assured by the transportation company. Damage claims must be filed immediately with the transportation company to receive compensation for damaged goods.
n LIMITED WARRANTY This instrument is guaranteed to be free from defects in material and workmanship for a period of two (2) years from original purchase date. In the event that a defect is found during the warranty time frame, LaMotte Company agrees that it will be repaired or replaced without charge except for the transporation costs. This guarantee does not cover batteries. This product can not be returned without a return authorization number from Lamotte Company.
n STATISTICAL AND TECHNICAL DEFINITIONS RELATED TO PRODUCT SPECIFICATIONS Method Detection Limit (MDL): “The method detection limit (MDL) is defined as the minimum concentration of a substance that can be measured and reported with 99% confidence that the analyte concentration is greater than zero and is determined from analysis of a sample in a given matrix containing the analyte.”1 Note that, “As Dr.
Sensitivity: Sensitivity is the resolution based on how this term is used in LaMotte catalogs. This term is not listed in any of the references. Sometimes it is used for detection limit. It is a confusing term and should be avoided. Repeatability: Repeatability is the within-run precision.6 A run is a single data set, from set up to clean up. Generally, one run occurs on one day.
n CE COMPLIANCE The SMART2 Colorimeter has earned the European CE Mark of Compliance for electromagnetic compatibility and safety.
CHEMICAL TESTING n WATER SAMPLING FOR CHEMICAL ANALYSIS n Taking Representative Samples The underlying factor to be considered for any type of water sampling is whether or not the sample is truly representative of the source. To properly collect a representative sample: l Sample as frequently as possible. n l Collect a large sample or at least enough to conduct whatever tests are necessary. l Make a composite sample for the same sampling area.
This weighted device is lowered to the sampling depth and allowed to rest at this depth for a few minutes. The water percolates into the sample chamber displacing the air which bubbles to the surface. When the bubbles cease to rise, the device has flushed itself approximately five times and it may be raised to the surface for examination. The inner chamber of the sampling device is lifted out and portions of the water sample are carefully dispensed for subsequent chemical analysis.
n AN INTRODUCTION TO COLORIMETRIC ANALYSIS Most test substances in water are colorless and undetectable to the human eye. To test for their presence we must find a way to “see” them. The SMART2 Colorimeter can be used to measure any test substance that is itself colored or can be reacted to produce a color. In fact a simple definition of colorimetry is “the measurement of color” and a colorimetric method is “any technique used to evaluate an unknown color in reference to known colors”.
n REAGENT BLANK Some tests will provide greater accuracy if a reagent blank is determined to compensate for any color or turbidity resulting from the reagents themselves. A reagent blank is performed by running the test procedure on 10 mL of demineralized water. Use sample water to SCAN BLANK. Insert the reagent blank in the colorimeter chamber and select SCAN SAMPLE. Note result of reagent blank. Perform the tests on the sample water as described.
Below is a list of suggested wavelengths for the color of the reacted samples. Use these as a starting point. Sample Color Wavelength Range Yellow 430 Pink 520 Red 570 Green and Blue 620 n CALIBRATION As with all pre-calibrated meters, it is highly recommended, even if not required by regulations, that the user periodically verify the performance of the meter by running standards with a predetermined concentration.
CALIBRATION CURVE CALIBRATION CURVE Absorbance vs. Concentration %T vs. Concentration 100 2.0 1.8 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2 0.0 %T Transmission Absorbance 3. Use the unreacted 0 ppm standard to standardize the colorimeter by using it to scan blank. 4. Following the individual reagent system instructions, react each standard solution beginning with 0 ppm. Continue with standards in increasing concentration. Record the reading and the standard solution concentration on a chart.
n PREPARING DILUTE STANDARD SOLUTIONS Standard solutions should be prepared to create a calibration curve. Standard solutions can be prepared by diluting a known concentrated standard by specified amounts. A chart or computer spreadsheet can be created to determine the proper dilutions. Use volumetric flasks and volumetric pipets for all dilutions. 1. In Column A – Record the maximum concentration of test as determined by the range and path length. 2.
concentration of iron due to the “spike” was (0.10 mL x 50 ppm)/10.0 mL = 0.50 ppm. The concentration of iron determined in the spiked sample should be 0.3 + 0.5 = 0.8 ppm iron. (Note: any error due to the increased volume from the “spike” is negligible). LaMotte offers a line of calibration standards which can be used to generate calibration curves and perform standard additions.
n INTERFERENCES LaMotte reagent systems are designed to minimize most common interferences. Each individual test instruction discusses interferences unique to that test. Be aware of possible interferences in the water being tested. The reagent systems also contain buffers to adjust the water sample to the ideal pH for the reaction. It is possible that the buffer capacity of the water sample may exceed the buffer capacity of the reagent system and the ideal pH will not be obtained.
OPERATION OF THE SMART2 COLORIMETER n OVERVIEW The SMART2 Colorimeter is a portable, microprocessor controlled, direct reading colorimeter. It has a graphical 4 line, 16 character liquid crystal display for graphical, alphabetical and numerical messages. The operation is controlled with the keypad through menu driven software in response to selections shown on the display. The test library consists of 100 LaMotte tests (not all 100 may be available at present) and 10 “User Tests”.
n COMPONENTS Figure 1 shows a diagram of the Smart2 Colorimeter and its components. Bottom View Top View Serial Number SMART2 •••••••••••••••••• * ENTER OFF EXIT ON Battery Compartment Side Views Lid AC Adapter Socket 20 RS232 Serial Port Figure 1 SMART2 COLORIMETER 1.
n QUICK START Some quick instructions to get into testing. 1. Press ON to turn on the SMART2. The LaMotte logo screen will appear for about 2 seconds and then the Start screen appears. Press Q/ENTER to start testing. VER 1.0 Smart2 * Start 2. The Main Menu will appear. Press Q/ENTER to select TESTING MENU. MAIN MENU * Testing Menu Editing Menu PC Link 3. Press Q/ENTER to select All Tests. TESTING MENU * All Tests Sequence 1 Sequence 2 4. Press t or s to move the * to the desired test.
8. Insert the reacted sample. Press Q/ENTER to scan sample. The SMART2 will scan the sample and display the concentration. 015 Chlorine * Scan Sample 9. After recording test result, scroll with t or s and make another selection with Q/ENTER. Press EXIT to escape to previous menus. 22 015 Chlorine 1.28 ppm * Scan Sample SMART2 COLORIMETER 1.
GENERAL OPERATING PROCEDURES The operation of the SMART2 Colorimeter is controlled by a microprocessor. The microprocessor is programmed with menu driven software. A menu is a list of choices. This allows a selection of various tasks for the colorimeter to perform, such as, scan blank, scan sample, and edit test sequences. The keypad is used to make menu selections which are viewed in the display. There are three selections accessible from the MAIN MENU: Testing Menu, Editing Menu and PC Link.
n THE DISPLAY & THE MENUS The display allows menu selections to be viewed and chosen. These choices instruct the colorimeter to perform specific tasks. The menus are viewed in the display using two general formats which are followed from one menu to the next. Each menu is a list of choices or selections. There are four lines in the display. The top line in each menu is a title or pertinent instruction. The top line does not change unless a new menu is selected.
The second general format of the display takes advantage of the graphics capabilities of the display. The top line of the display is still a title line. The middle two lines of the display are used to display important messages, results or graphics in a large, easy to read format. The menus work in the same way as described previously but only one line of the menu is visible at the bottom of the display.
n LOOPING MENUS Long menus, such as All Tests, incorporate a looping feature which allow the user to quickly reach the last choice in the menu from the first choice. In a looping menu the last choices in the menu are above the first choice and scrolling upward moves through the menu in reverse order. Scrolling downward moves through the menu from first choice to last but the menu starts over following the last choice. So all menu choices can be reached by scrolling in either direction.
TESTING n TESTING MENU The Testing Menu is used to run all LaMotte pre-programmed tests, USER TESTS and Absorbance tests at one of four wavelengths. Testing from any of three sequences can also be done. 1. Press the ON button to turn on the SMART2 Colorimeter. The LaMotte logo will appear for about 2 seconds and the the Start screen appears. Press the Q/ENTER button to begin testing. VER 1.0 2. The MAIN MENU will appear. Press the Q/ENTER button to select Testing Menu.
n SEQUENCES OF TESTS SEQUENCE 1, SEQUENCE 2, and SEQUENCE 3 are alterable sequences. They may be edited using the Editing Menu. Any of the LaMotte pre-programmed tests or User Tests may be placed in these sequences in whatever testing order that is preferred. Some examples of typical sequences are given below.
n GENERAL TESTING PROCEDURES The following are some step by step examples of how to run tests from the Testing Menu. These test procedures are designed to be used with LaMotte SMART Reagent Systems. LaMotte Company continuously updates the list of pre-programmed tests as the calibrations become available. Pre-programmed calibrations can be added to the SMART2 Colorimeter in the field.
Press the Q/ENTER button to select 002 Aluminum. ALL TESTS * 002 Aluminum 003 Ammonia - NLF 004 Ammonia - NLS The SMART2 Colorimeter is ready to scan at the correct wavelength. Place the blank in the sample chamber, close the lid and press the Q/ENTER button to scan blank. NOTE: Do not keep the button depressed. 002 Aluminum The screen will display Blank Done for about 1 second. Scan Sample will be positioned next to *.
n CALIBRATING LaMOTTE PRE-PROGRAMMED TESTS The LaMotte Pre-Programmed Tests have been pre-calibrated. Recalibration of the pre-programmed tests by the user is not possible. However, a procedure to standardize the calibration can be performed to obtain the most accurate readings or to meet regulatory requirements. The LaMotte Pre-Programmed tests are standardized with one standard solution.
A reverse font (dark background with light characters) will appear to indicate that the reading can be adjusted. Use s or t to scroll to the concentration of the sample, 0.10 ppm in this example. 002 Alumninum Set the calibration by pressing Q/ENTER to select Calibrate. 002 Aluminum 0.09 * Calibrate 0.10 * Calibrate Two menu choices will be offered, Set Calibration and Factory Setting.
n MEASURING IN THE ABSORBANCE MODE Press ON to turn on the SMART2 Colorimeter. The LaMotte logo will appear for about 2 seconds and then the Start screen appears. Press the Q/ENTER button to start testing. VER 1.0 The MAIN MENU will appear. Press the Q/ENTER button to select Testing Menu. MAIN MENU * Testing Menu Editing Menu PC Link Press the t button to scroll to Absorbance. TESTING MENU All Tests Sequence 1 Sequence 2 Sequence 3 * Absorbance Press the Q/ENTER button to select Absorbance.
Insert blank, press the Q/ENTER button to scan blank. 102 Abs 520 * Scan Blank The screen will display Blank Done for about 1 second. 102 Abs 520 Blank Done * Scan Blank Insert the reacted sample. Press the Q/ENTER button to scan the sample. 102 Abs 520 * Scan Sample Record test result. To repeat the test, press the Q/ENTER button to scan the sample again. The last blank scanned is used to zero the colorimeter for repeated scans.
EDITING MENU The EDITING MENU allows the user to edit sequences, edit user tests, set the clock, edit the logging function, and set the power saving function. n EDIT A SEQUENCE The EDIT SEQUENCE menu allows three alterable test sequences (SEQUENCE 1, SEQUENCE 2, and SEQUENCE 3) to be edited. Press ON to turn on the SMART2 Colorimeter. The LaMotte logo will appear for about 2 seconds and then the Start screen appears. Press the Q/ENTER button to start testing. VER 1.0 The Main Menu will appear.
Sequence 1 appears. EDIT SEQUENCE 1 *015 Chlorine 079 Phosphate H 009 Bromine-LR n ADDING OR DELETING TESTS There are three ways to alter a sequence: Insert Before, Insert After, and Delete. Insert Before adds a new test to the sequence before the selected test. Insert After adds a new test to the sequence after the selected test. Delete is used to remove an existing test from a sequence. Below is a step by step example of how to add a test to SEQUENCE 1 starting from the EDIT SEQUENCE 1 menu.
Press the Q/ENTER button to select 002 Aluminum. ALL TESTS * 002 Aluminum 003 Ammonia-N LF 004 Ammonia-N LS Sequence 1 appears in EDIT SEQUENCE 1 menu and 002 Aluminum is now before Bromine-LR in the sequence. All changes to Sequence 1 are automatically saved. Press the EXIT button to exit the EDIT SEQUENCE 1 menu and return to the EDIT SEQUENCE menu or continue editing. EDIT SEQUENCE 1 * 015 Chlorine 079 Phosphate H 002 Aluminum 009 Bromine-LR 076 pH TB 060 Moly-LR The EDIT SEQUENCE menu appears.
Press the t button to scroll to Delete. EDIT SEQUENCE 1 * Insert Before Insert After Delete Press the Q/ENTER button to select Delete. EDIT SEQUENCE 1 * Delete Sequence 1 appears in the EDIT SEQUENCE 1 menu and 002 Aluminum has been deleted. All changes to SEQUENCE 1 are automatically saved. Press the EXIT button to exit the EDIT SEQUENCE 1 menu and return to the EDIT SEQUENCE menu or continue editing.
n EDIT USER TESTS If a test other than the LaMotte programmed tests is performed regularly, a calibration for it may be entered in one of the 10 User Tests. These tests are originally named “User Test 1 - 10". It will be possible to rename the test, select a wavelength, enter a new calibration, select the number of decimal places used to display the results, and select the units. A User Test may be added for a reagent system for which no precalibrated test exists.
n NAMING THE TEST A User Test can be up to 11 characters long. The menu choices for each character are 26 upper case letters A to Z, 26 lower case letters a to z, ten numerals 0 to 9, a space (SP), a dash (-) and a decimal point (.). The existing name is displayed on the bottom line of the display. A cursor will be over the character which is to be edited and that character is also displayed in the center of the display.
The number “2” has been entered in the second position of the name and the cursor has moved to the third letter “e”. NAME THE TEST e 105 H2er Test01 Use the t and s buttons to scroll to the letter “O” into the center of the display. Press the Q/ENTER button to select the letter “O”. NAME THE TEST The letter “O” has been entered in the third position of the name and the cursor has moved to the fourth letter “r”. Press the EXIT button to save the name entered up to this point.
n SELECTING THE VIAL AND WAVELENGTH The Smart2 Colorimeter has three different vials (the 25 mm 0290 tube, UDVs and COD tubes) and 4 different wavelengths (430, 520, 570, and 620 nm). The colorimeter uses different settings for each of the twelve combinations of vial and wavelength. These twelve settings are called channels. Choose the channel with the correct wavelength and vial for the test. Use the t button to scroll to Select Vial/WL and press Q/ENTER button to select.
n ENTERING A NEW CALIBRATION To enter a new calibration two reacted standards solutions of known concentration are required: a “low standard” and a “high standard”. These should be ready to use. Use the t button to scroll to New Calibration and press Q/ENTER button to select.
Place a clear blank in the sample chamber. Press the Q/ENTER button to scan the blank. Insert Blank * Continue The screen will display Blank Done for about 1 second. Blank Done * Scan Blank Place the reacted low standard in the sample chamber. Press Q/ENTER to scan the low standard. Insert Lo Standard * Continue Place the reacted high standard in the sample chamber. Press Q/ENTER to scan the high standard.
n SELECTING THE NUMERICAL FORMAT OF THE RESULT To input tests with very different ranges, the number of decimal places displayed for a result can be selected. A test which ranges from 20 to 1000 ppm should not be displayed with three decimal places. A test with a range from 0.010 to 0.500 needs three decimal places (the microprocessor will always calculate the concentration to many more significant figures than will be displayed). Menu choices of 0, 1, 2, or 3 decimal places will be given for the display.
n SELECTING THE UNITS OF CONCENTRATION The SMART2 Colorimeter has seven options for units of concentration. They are No Units, ppm, pH, FTU, ppb, ppt and mgL. Use the t button to scroll to Select Units and press Q/ENTER to select. EDIT USER TEST01 * Decimal Places Select Units Use the t button to scroll to the appropriate unit and press Q/ENTER to select. SELECT UNITS * No Units ppm pH FTU ppb ppt mgL The meter will display the message “Storing” and return to the EDIT USER TEST01 menu.
n SETTING THE CLOCK Setting the clock allows the correct time and date stamp to be stored with each reading in the data logger and with each reading sent out the serial port. From the EDITING MENU use the t button to scroll to Set Clock. Press Q/ENTER to select. EDITING MENU * Edit Sequences Edit User Test Set Clock Editing Logging Factory Setup Set PWR Save The current date and time are displayed as month - day - year on the first line and as hours : minutes : seconds on the second line.
n TURNING THE DATA LOGGER ON AND OFF The default setting for the datalogger is “Enabled” or turned off. If there is no need for data logging, this setting is suggested. If data logging is needed, the data logger can be “Enabled” or turned on. From the EDITING MENU use the t button to scroll to Edit Logging. Press Q/ENTER to select. EDITING MENU * Edit Sequences Edit User Test Set Clock Edit Logging Factory Setup Set PWR Save The current setting is always displayed next to the *.
n FACTORY SETUP The Factory Setup menu is used in the manufacturing of the SMART2 Colorimeter. This menu is not for use by the operator in the field. n SETTING THE POWER SAVING FUNCTION The SMART2 Colorimeter has a power saving function that turns the meter off after an interval of inactivity. If no buttons have been pressed during that interval the meter will turn itself off. This interval can be disabled or set for 5, 15, 30 or 60 minutes. The default setting is 5 minutes.
PC LINK The SMART2 Colorimeter may be interfaced with any Windows-based computer by using the LaMotte SMARTLink2 Program and Interface Cable (Order Code 1912-3 [3.5 disk] or 1912-CD [compact disk]). The program stores customer information and test data in a database. It can be used to download data stored in the Smart2 datalogger for each test site.
MAINTENANCE n CLEANING Clean with a damp, lint-free cloth. DO NOT ALLOW WATER TO ENTER THE COLORIMETER CHAMBER OR ANY OTHER PARTS OF THE METER. n METER CARE The optical system of the SMART2 must be kept clean and dry for optimal performance. Dry the colorimeter tubes before placing them in the chamber to avoid introducing moisture. For best results store the instrument in an area that is dry and free from aggressive chemical vapors.
TROUBLESHOOTING GUIDE n ERROR MESSAGES n OVER RANGE If the message OVERRANGE is displayed when scanning a sample, the sample may be over range or under range. If the sample is over range the sample should be diluted and tested again (see Sample Dilution Techniques and Volumetric Measurements, page 16). If OVERRANGE is displayed, press the Q/ENTER button to continue testing on diluted samples.
n HELPFUL HINTS n STRAY LIGHT The SMART2 Colorimeter should have no problems with stray light. Make sure that the sample compartment lid is always fully closed, except when testing COD with the adapter. SMART2 COLORIMETER 1.
SMART2 COLORIMETER 1.
SMART 2 ••••••••••••••••••••••••••••••••••••••••••••••• Colorimeter REAGENT SYSTEMS TEST INSTRUCTIONS v.2.4 • Printed 08.
SMART2 COLORIMETER REAGENT SYSTEMS SMART2 REAGENT SYSTEMS LIST LaMotte Company continuously updates the list of pre-programmed tests as the calibrations become available. Pre-programmed calibrations can be added to the Smart2 Colorimeter in the field. A Windows-based computer running a Windows Operating System and an 8 pin mini-DIN/9 pin F D-submin serial cable (order Code 1771) are required. Call LaMotte Technical Services at 1-800-344-3100 (410-778-3100 outside the USA) or email at tech@lamotte.
Test Factor (Test #) Range (ppm) Test Method (# of Reagents) # of Tests Cobalt (24) 0.00–2.00 PAN (3) 50 COD-Low Range (25) 5–150 Digestion (1) 25 COD-Standard Range (26) 0-1500 Digestion (1) 25 COD-High Range (27) 0–15000 Digestion (1) 25 Color (28) 0–1000 Platinum Cobalt (0) ¥ Copper-BCA-Low Range (29) 0.00–3.50 Bicinchoninic Acid (1) 50 Copper-Cuprizone (31) 0.00–2.00 Cuprizone (2) 50 Copper-DDC (32) 0.00–6.00 Diethyldithiocarbamate (1) 50 Copper-UDV (33) 0.0–4.
Test Factor (Test #) Range (ppm) Test Method (# of Reagents) # of Tests Methylethylketoxime (58) See Oxygen Scavengers 0.00–3.00 Iron Reduction (3) 100 Molybdenum-High Range (61) 0.0–50.0 Thioglycolate (3) 50 Nickel (63) 0.00–8.00 Dimethylglyoxime (6) 50 Nitrate Nitrogen-Low Range (64) 0.00–3.00 Cadmium Reduction (2) 20 Nitrate-TesTab (66) 0.0–60.0 Zinc Reduction (1) 50 Nitrite Nitrogen-Low Range (67) 0.00–0.80 Diazotization (2) 20 Nitrite-TesTab (69) 0.00–1.
ALKALINITY–UDV UNIT DOSE VIALS • CODE 4318-H QUANTITY CONTENTS 1 Alkalinity Unit Dose Vials, 10 pouches CODE 4318-H Equipment needed but not supplied: STANDARD ACCESSORY PACKAGE · CODE 1961 1 Package of 3 Vials (empty) 0156 1 Syringe, 3 mL, plastic 1184 1 Foil Storage Bag 9467 Or: ADVANCED ACCESSORY PACKAGE · CODE 1962 1 Pipettor, 3mL 30528 1 Pipet Tip (0-5 mL) 30695 1 Cuvette Rack 31695 1 Package of 3 Vials (empty) 0156 1 Foil Storage Bag 9467 APPLICATION: RANGE: METHOD: Dr
PROCEDURE Use 10 mm square cell adapter 1. Press and hold ON button until colorimeter turns on. 2. Press ENTER to start. 3. Press ENTER to select TESTING MENU. 4. Select ALL TESTS (or another sequence containing 1 ALKALINTIY-UDV) from TESTING MENU. 5. Scroll to and select 1 ALKALINITY-UDV from menu. 6. Rinse a clean vial (0156) with sample water. 7. Use the syringe (1184) to add 3 mL of sample to the vial. 8. Insert the vial into chamber, close lid and select SCAN BLANK. 9. Remove vial from the colorimeter.
ALUMINUM ERIOCHROME CYANINE R METHOD • CODE 364I-SC QUANTITY CONTENTS 5g *Aluminum Inhibitor Reagent 2 x 120 mL *Aluminum Buffer Reagent CODE *7865-C *7866-J 120 mL Aluminum Indicator Reagent 7867-J 15 mL Aluminum Complexing Reagent 7868-E 1 Spoon, 0.05 g, plastic 0696 2 Pipets, 1.0 mL, plastic 0354 1 Test Tube, glass, 5 mL w/cap 0230 *WARNING: Reagents marked with an * are considered to be potential health hazards.
PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 2 Aluminum). Scroll to and select 2 Aluminum from menu. Rinse a clean colorimeter tube (0290) with sample water. Fill to the 10 mL line with sample. Insert tube into colorimeter chamber and select SCAN BLANK. Rinse a clean test tube (0230) with sample water.
AMMONIA-NITROGEN - LOW RANGE SALICYLATE METHOD • CODE 3659-01-SC QUANTITY CONTENTS CODE 60 mL *Salicylate Ammonia #1 *3978-H 10 g *Salicylate #2 *7457-D 2x5g *Salicylate #3 Reagent Powder *7458-C 1 Spoon, 0.1 g, plastic 0699 1 Spoon, 0.15 g, plastic 0727 1 Pipet, 1.0 mL, plastic 0354 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com.
PROCEDURE - FRESH WATER Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 3 Ammonia-NLF) from TESTING MENU. 5. Scroll to and select 3 Ammonia-NLF from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. (See Note.) 8. Remove tube from colorimeter. Use the 1.0 mL plastic pipet (0354) to add 2.
PROCEDURE - SALT WATER Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 4 Ammonia-NLS) from TESTING MENU. 5. Scroll to and select 4 Ammonia-NLS from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. (See Note.) 8. Remove tube from colorimeter. Use the 1.0 mL plastic pipet (0354) to add 2.
Smart2 TEST PROCEDURES 2.
AMMONIA-NITROGEN HIGH RANGE NESSLERIZATION METHOD • CODE 3642-SC QUANTITY CONTENTS 30 mL Ammonia Nitrogen Reagent #1 V-4797-G *Ammonia Nitrogen Reagent #2 *V-4798-G 2 x 30 mL 1 Pipet, 1 mL, plastic CODE 0354 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Scroll to and select ALL TESTS (or another sequence containing 5 Ammonia-N H) from TESTING MENU. 5. Scroll to and select 5 Ammonia-N H from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. (See Note) 8. Remove tube from colorimeter. Add 8 drops of Ammonia Nitrogen Reagent #1 (V-4797).
BENZOTRIAZOLE/TOLYLTRIAZOLE UV Photolysis Method • CODE 4047 QUANTITY 15 g 25 mL 25 mL CONTENTS CODE *Benzotriazole Reagent *3818-E Potassium Sodium Tartrate Solution *Sulfuric Acid 7841WT-G *6139WT-G 1 pH Test Papers, 1–11 2956 1 Spoon, 0.25 g, plastic 0695 1 Erlenmeyer Flask, 25 mL, glass 1 Graduated Cylinder, 25 mL, glass 2-2109 0417 *WARNING: Reagents marked with an * are considered to be potential health hazards.
SAMPLE Samples should be analyzed as soon as possible after HANDLING & collection. PRESERVATION: INTERFERENCES: Tolyltriazole with interfere in the benzotriazole test. Benzotriazole will interfere in the tolyltriazole test. Strong reducing or oxidizing agents will interfere. Benzotriazole/Tolyltriazole 2/4 Smart2 TEST PROCEDURES 4.
BENZOTRIAZOLE PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select Testing Menu. Select ALL TESTS (or another sequence containing 10 B triazole from TESTING MENU. Scroll to and select 10 B triazole from menu. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. Insert the tube into chamber, close lid and select SCAN BLANK. Remove the tube from colorimeter. Discard the sample.
TOLYLTRIAZOLE PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select Testing Menu. Select ALL TESTS (or another sequence containing 97 T triazole from TESTING MENU. Scroll to and select 97 T triazole from menu. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. Insert the tube into chamber, close lid and select SCAN BLANK. Remove the tube from colorimeter. Discard the sample.
BIGUANIDE COLORIMETRIC METHOD • CODE 4044 QUANTITY CONTENTS 2 X 60 mL Biguanide Indicator 1 Pipet, plastic, 1.0 mL CODE 3994-H 0354 Biguanide is a non-chlorine, non-bromine chemical sanitizer. It is more stable than chlorine or bromine and has little chemical odor. Biquanide is an effective bacteriacide but, unlike chlorine and bromine, it does not destroy organic contaminants. Therefore, hydrogen peroxide is added to biguanide pools on a regular basis to eliminate organic contaminants.
PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select Testing Menu. Select ALL TESTS (or another sequence containing 7 Biguanide from TESTING MENU. Scroll to and select 7 Biguanide from menu. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. Insert the tube into chamber, close lid and select SCAN BLANK. Remove the tube from colorimeter. Use the 1.0 mL pipet (0354) to add 2.
BORON AZOMETHINE-H METHOD · CODE 4868 QUANTITY CONTENTS CODE 120 mL *Boron Buffer *4869-J 10 g *Boron Indicator Powder *4870-D 1 Pipet, plastic, 1.0 mL 0354 1 Spoon, 0.15 g 0727 1 Dark storage chamber, brown 0108 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax..
PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. This test requires a Reagent Blank. Rinse a tube (0290) with clear, colorless, boron free water. Fill to 10 mL line with clear, colorless, boron free water. Use the 1.0 mL pipet (0354) to add 2 mL of *Boron Buffer (4869). Cap and mix. Use the 0.15 g spoon (0727) to add one level measure of *Boron Indicator Powder (4870). Press full spoon against side of jar to compress powder.
BROMINE - UDV DPD METHOD–UNIT DOSE VIALS · CODE 4311-H QUANTITY 1 CONTENTS *Free Chlorine Unit Dose Vials, 10 pouches CODE *4311-H *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
INTERFERENCE: The only interfering substance likely to be encountered in water is oxidized manganese. The extent of this interference can be determined by treating a sample with sodium arsenite to destroy the bromine present so that the degree of interference can be estimated. Iodine and chlorine can also interfere, but these are not normally present unless they have been added as sanitizers. Bromine - UDV 2/3 Smart2 TEST PROCEDURES 2.
PROCEDURE Use 10 mm square cell adapter. 1. Press and hold ON button until colorimeter turns on. 2. Press ENTER to start. 3. Press ENTER to select Testing Menu. 4. Select ALL TESTS (or another sequence containing 11 Bromine-UDV) from TESTING MENU. 5. Scroll to and select 11 Bromine-UDV from menu. 6. Rinse a clean vial (0156) with sample water. 7. Use the syringe (1184) to add 3mL of sample to the vial. 8. Insert the vial into chamber, close the lid and select SCAN BLANK. 9.
Smart2 TEST PROCEDURES 2.
CADMIUM PAN METHOD • CODE 4017 QUANTITY 60 mL 15 mL 30 mL CONTENTS *Buffered Ammonia Reagent Sodium Citrate, 10% *PAN Indicator CODE *4020-H 6253-E *4021-G 30 mL Stabilizing Reagent 4022-G 1 Pipet, 1.0 mL, plastic 0354 2 Pipet, 0.5 mL, plastic 0369 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 12 Cadmium) from TESTING MENU. Scroll to and select 12 Cadmium from menu. Rinse a tube (0290) with sample water. Fill to the 10 mL line with sample. Insert the tube into chamber, close lid and select SCAN BLANK. Remove tube from colorimeter. Use the 1.0 mL pipet (0354) to add 1.
CALCIUM & MAGNESIUM (TOTAL) HARDNESS–UDV UNIT DOSE VIALS • CODE 4309-H QUANTITY CONTENTS 1 Calcium Hardness Unit Dose Vials, 10 pouches CODE 4309-H Equipment needed but not supplied: STANDARD ACCESSORY PACKAGE · CODE 1961 1 Package of 3 Vials (empty) 0156 1 Syringe, 3 mL, plastic 1184 1 Foil Storage Bag 9467 Or: ADVANCED ACCESSORY PACKAGE · CODE 1962 1 Pipettor, 3 mL 30528 1 Pipet Tips (0-5 mL) 30695 1 Cuvette Rack 31695 1 Package of 3 Vials (empty) 0156 1 Foil Storage Bag 9467
PROCEDURE Use 10 mm square cell adapter. 1. Press and hold ON button until colorimeter turns on. 2. Press ENTER to start. 3. Press ENTER to select TESTING MENU. 4. Select ALL TESTS (or another sequence containing 13 Ca&Mg H-UDV) from TESTING MENU. 5. Scroll to and selec t 13 Ca&Mg Hard-UDV from menu. 6. Rinse a clean vial (0156) with sample water. 7. Use the syringe (1184) to add 3 mL of sample to the vial. 8. Insert the vial into chamber, close lid and select SCAN BLANK. 9.
CHLORIDE ARGENTOMETRIC METHOD · CODE 3693-SC QUANTITY 50 1 CONTENTS *Chloride Spectrophotometric Grade Tablets Tablet Crusher CODE *3885A-H 0175 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. Chloride is one of the major anions found in water and sewage.
PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 21 Chloride-TT) from TESTING MENU. Scroll to and select 21 Chloride-TT from menu. Rinse a tube (0290) with sample water. Fill to 10 mL line with sample. Insert the tube into chamber, close lid and select SCAN BLANK. Remove the tube from colorimeter.
CHLORINE LIQUID DPD METHOD · CODE 4859 QUANTITY CONTENTS CODE 30 mL DPD 1A Free Chlorine Reagent P-6740-G 30 mL *DPD 1B Free Chlorine Reagent *P-6741-G 30 mL *DPD 3 Total Chlorine Reagent *P-6743-G *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
SAMPLE HANDLING & PRESERVATION: INTERFERENCE: Chlorine in aqueous solutions is not stable, and the chlorine content of samples or solutions, particularly weak solutions, will rapidly decrease. Exposure to sunlight or agitation will accelerate the reduction of chlorine present in such solutions. For best results, start analysis immediately after sampling. Samples to be analyzed for chlorine cannot be preserved or stored.
PROCEDURE–FREE CHLORINE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select Testing Menu. Select ALL TESTS (or another sequence containing 17 Cl DPD-Liq) from TESTING MENU. 5. Scroll to and select 17 Cl DPD-Liq from menu. 6. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 7. Insert the tube into chamber, close lid and select SCAN BLANK. 8. Remove the tube from colorimeter. 9. Add 5 drops of DPD 1A Free Chlorine Reagent (P-6740). 10.
Smart2 TEST PROCEDURES 2.
CHLORINE–BROMINE–IODINE DPD METHOD • CODE 3643-SC QUANTITY CONTENTS CODE 100 *DPD #1 Instrument Grade Tablets *6903A-J 100 *DPD #3 Instrument Grade Tablets *6197A-J 15 mL Glycine Solution 6811-E 1 Tablet Crusher 0175 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
SAMPLE HANDLING & PRESERVATION: INTERFERENCE: Chlorine in aqueous solutions is not stable, and the chlorine content of samples or solutions, particularly weak solutions, will rapidly decrease. Exposure to sunlight or agitation will accelerate the reduction of chlorine present in such solutions. For best results, start analysis immediately after sampling. Samples to be analyzed for chlorine cannot be preserved or stored.
PROCEDURE–FREE CHLORINE 1. 2. 3. 4. 5. 6. 7. 8. 9. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 15 Chlorine) from TESTING MENU. Scroll to and select 15 Chlorine from menu. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. Insert tube into chamber, close lid and select SCAN BLANK.
BROMINE Like chlorine, bromine is an effective germicidal agent employed in drinking water treatment, pool and spa water sanitization, food service sanitation, and other public health applications. APPLICATION: Drinking, surface, and saline waters; swimming pool water; domestic and industrial waters and wastes. RANGE: 0.00–9.00 Bromine METHOD: In buffered sample bromine reacts with diethyl-p-phenylene diamine (DPD) to produce a pink-red color in proportion to the concentration of bromine present.
PROCEDURE A: BROMINE (NO CHLORINE) Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 9 Bromine-LR) from TESTING MENU. 5. Scroll to and select 9 Bromine-LR from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter.
PROCEDURE B: BROMINE IN THE PRESENCE OF CHLORINE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 9 Bromine-LR) from TESTING MENU. 5. Scroll to and select 9 Bromine-LR from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber close lid and select SCAN BLANK. 8. Rinse a second clean tube (0290) with sample water.
IODINE Like chlorine and bromine, iodine is an effective germicidal agent employed in drinking water treatment, pool and spa water sanitization, food service sanitation, and other public health applications. APPLICATION: Drinking, surface, and saline waters; swimming pool water; domestic and industrial wastes. RANGE: 0.00–14.00 ppm Iodine METHOD: In a buffered sample iodine reacts with diethyl-p-phenylene-diamine (DPD) to produce a pink-red color in proportion to the concentration of iodine present.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 50 Iodine) from TESTING MENU. 5. Scroll to and select 50 Iodine from menu. 6. Rinse a clean tube (0290) with sample water. Fill tube to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter. Pour off all but a sufficient amount of sample water to cover a tablet.
CHLORINE, FREE - UDV DPD METHOD–UNIT DOSE VIALS · CODE 4311-H QUANTITY 1 CONTENTS CODE *Free Chlorine Unit Dose Vials, 10 pouches *4311-H *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
Chlorine can be added in the form of chlorine gas, liquid sodium hypochlorite (bleach), granular calcium hypochlorite or as organic chlorine compounds. Chlorine is not present in natural water supplies; if it is present it is the result of chlorination of a water supply or of chlorinated compounds being discharged as waste from industrial operations. The presence of chlorine in concentrations above 0.
PROCEDURE Use 10 mm square cell adapter. 1. Press and hold ON button until colorimeter turns on. 2. Press ENTER to start. 3. Press ENTER to select Testing Menu. 4. Select ALL TESTS (or another sequence containing 16 Cl Free-UDV) from TESTING MENU. 5. Scroll to and select 16 Cl Free-UDV from menu. 6. Rinse a clean vial (0156) with sample water. 7. Use the syringe (1184) to add 3mL of sample to the vial. 8. Insert the vial into chamber, close the lid and select SCAN BLANK. 9.
Smart2 TEST PROCEDURES 2.
CHLORINE, TOTAL - UDV DPD METHOD–UNIT DOSE VIALS · CODE 4312-H QUANTITY 1 CONTENTS CODE *Total Chlorine Unit Dose Vials, 10 pouches *4312-H *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
Chlorine can be added in the form of chlorine gas, liquid sodium hypochlorite (bleach), granular calcium hypochlorite or as organic chlorine compounds. Chlorine is not present in natural water supplies; if it is present it is the result of chlorination of a water supply or of chlorinated compounds being discharged as waste from industrial operations. The presence of chlorine in concentrations above 0.
PROCEDURE Use 10 mm square cell adapter. 1. Press and hold ON button until colorimeter turns on. 2. Press ENTER to start. 3. Press ENTER to select Testing Menu. 4. Select ALL TESTS (or another sequence containing 18 Cl Total-UDV) from TESTING MENU. 5. Scroll to and select 18 Cl Total-UDV from menu. 6. Rinse a clean vial (0156) with sample water. 7. Use the syringe (1184) to add 3mL of sample to the vial. 8. Insert the vial into chamber, close the lid and select SCAN BLANK. 9.
Smart2 TEST PROCEDURES 2.
CHLORINE DIOXIDE DPD METHOD • CODE 3644-SC QUANTITY 100 CONTENTS *DPD #1 Instrument Grade Tablets 15 mL Glycine Solution 1 Tablet Crusher CODE *6903A-J 6811-E 0175 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. Chlorine dioxide is used as a substitute for and an adjunct to chlorine in water treatment.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 20 CHLOR DIOX) from TESTING MENU. 5. Scroll to and select 20 CHLOR DIOX from menu. 6. Rinse a clean tube (0290) with sample water. Fill to 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter. Pour out all but a sufficient amount of sample water to cover tablet.
CHROMIUM DIPHENYLCARBOHYDRAZIDE METHOD • CODE 3697-SC QUANTITY 50 1 CONTENTS CODE *Chromium Spectrophotometric Grade Tablets Tablet Crusher *3889A-H 0175 **WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. Chromium is one of a class of heavy metals found in the bottom mud of polluted bodies of water.
PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 23 Chromium-TT) from TESTING MENU. Scroll to and select 23 Chromium-TT from menu. Rinse a tube (0290) with sample water. Fill to 10 mL line with sample. Insert the tube into chamber, close lid and select SCAN BLANK. Remove the tube from colorimeter.
CHROMIUM-HEXAVALENT DIPHENYLCARBOHYDRAZIDE METHOD • CODE 3645-SC QUANTITY 10 g CONTENTS *Chromium Reagent Powder 1 Spoon, 0.1 g, plastic 50 Filter Paper 1 Funnel, Plastic CODE *V-6276-D 0699 0465-H 0459 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 22 Chromium) from TESTING MENU. 5. Scroll to and select 22 Chromium from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter. Use the 0.1g spoon (0699) to add one measure of *Chromium Reagent Powder (V-6276).
CHROMIUM–HEXAVALENT, TRIVALENT & TOTAL DIPHENYLCARBOHYDRAZIDE METHOD • CODE 3698-SC QUANTITY CONTENTS CODE 60 mL *Sulfuric Acid, 5N *7681-H 10 g *Chromium Reagent Powder 15 mL *Sodium Azide, 5% *V-6276-D *7683-E 30 mL Potassium Permanganate, 0.5% 7682-G 60 mL Deionized Water 1 Pipet, plain, glass, w/cap 0341 1 Pipet, 1.0 mL, plastic 0354 1 Spoon, 0.
SAMPLE HANDLING & PRESERVATION: INTERFERENCES: Analysis for chromium should be made as quickly as possible after sample collection since storage in glass or plastic containers may result in low chromate values. High concentrations of mercurous and mercuric ions may interfere. HEXAVALENT CHROMIUM PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 22 Chromium) from TESTING MENU. 5.
TOTAL CHROMIUM WITH ACID DIGESTION PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. Fill graduated cylinder (0418) to 50 mL line with sample water. Transfer to Erlenmeyer flask (0431). Use the 1 mL pipet (0354) to add 5 mL (five measures) of *Sulfuric Acid, 5N (7681). Swirl to mix. þ NOTE: Highly buffered waters may require pH adjustment. Adjust the pH of highly buffered samples to 7.0 ±0.5. Continue procedure. Place flask on burner or hot plate.
TRIVALENT CHROMIUM PROCEDURE Subtract hexavalent chromium from total chromium. Record as ppm trivalent chromium. Trivalent Chromium = Total Chromium – Hexavalent Chromium Chromium–Hexavalent, Trivalent & Total 4/4 Smart2 TEST PROCEDURES 10.
COBALT PAN METHOD · CODE 4851 QUANTITY CONTENTS CODE 60 mL *Cobalt Buffer *4852-H 60 mL *Cobalt Indicator Reagent *4853-H 30 mL *Stabilizer Solution *4854-G 2 Pipet, 1.0 mL, plastic 0354 1 Pipet, 0.5 mL, plastic 0353 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select All Tests (or another sequence containing 24 Cobalt) from TESTNG MENU. Scroll to and select 24 Cobalt from menu. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. Insert the tube into chamber, close lid and select SCAN BLANK. Remove the tube from colorimeter. Use the 1.
COD–LOW RANGE MERCURY FREE DIGESTION • CODE 0072-SC MERCURY DIGESTION • CODE 0075-SC QUANTITY CONTENTS CODE 25 *COD Low Range Mercury Free Tubes *0072-SC or 25 *COD Low Range Mercury Tubes *0075-SC *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. COD Low Range Mercury Free Tubes are not USEPA approved.
INTERFERENCES: Volatile organic compounds are not oxidized to the extent that they are in the vapor above the digestion solution. Therefore, they do not contribute to the COD reading. Chloride concentrations above 10% of COD interfere with the mercury free tubes. Chloride above 2000 ppm will interfere with the mercury tubes. Nitrite gives a positive interference of 1.1 ppm O2 per ppm NO2–N which is insignificant unless nitrite concentrations are very high.
PROCEDURE Use COD adapter (see p. 22). 1. Homogenize sample if necessary. 2. Preheat COD heater block to 150±2°C. 3. Remove cap from COD tube. Hold tube at a 45° angle. Use a volumetric pipet, to carefully add 2.0 mL sample water allowing the sample to run down the side of the tube. 4. Cap and mix thoroughly. 5. Rinse the outside of the tube with distilled water. Wipe dry with a paper towel. 6. Repeat steps 3 through 5 using 2.0 mL distilled water. This is the reagent blank. 7.
þ NOTES: Reagents are light sensitive. Unused reagents should be stored in the shipping container, and in the refrigerator if possible, until needed. A reagent blank should be run with each set of samples and with each lot of reagents. The reacted blank will be stable if stored in the dark. To eliminate error caused by contamination, wash all glassware with 20% sulfuric acid. For greater accuracy, a minimum of three repetitions should be performed and the results averaged.
COD–STANDARD RANGE MERCURY FREE DIGESTION • CODE 0073-SC MERCURY DIGESTION • CODE 0076-SC QUANTITY CONTENTS CODE 25 *COD Standard Range Mercury Free Tubes *0073-SC or 25 *COD Standard Range Mercury Tubes *0076-SC *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
INTERFERENCES: Volatile organic compounds are not oxidized to the extent that they are in the vapor above the digestion solution. Therefore, they do not contribute to the COD reading. Chloride concentrations above 10% of COD interfere with the mercury free tubes. Chloride above 2000 ppm will interfere with the mercury tubes. Nitrite gives a positive interference of 1.1 ppm O2 per ppm NO2–N which is insignificant unless nitrite concentrations are very high.
PROCEDURE Use COD adapter (see p. 22). 1. Homogenize sample if necessary. 2. Preheat COD heater block to 150±2°C. 3. Remove cap from COD tube. Hold tube at a 45° angle. Use a volumetric pipet, to carefully add 2.0 mL sample water allowing the sample to run down the side of the tube. 4. Cap and mix thoroughly. 5. Rinse the outside of the vial with distilled water. Wipe dry with a paper towel. 6. Repeat steps 2 through 5 using 2.0 mL distilled water. This is the reagent blank. 7.
þ NOTES: Reagents are light sensitive. Unused reagents should be stored in the shipping container, and in the refrigerator if possible, until needed. A reagent blank should be run with each set of samples and with each lot of reagents. The reacted blank will be stable if stored in the dark. To eliminate error caused by contamination, wash all glassware with 20% sulfuric acid. For greater accuracy, a minimum of three repetitions should be performed and the results averaged.
COD–HIGH RANGE MERCURY FREE DIGESTION • CODE 0074-SC MERCURY DIGESTION • CODE 0077-SC QUANTITY CONTENTS CODE 25 *COD High Range Mercury Free Tubes *0074-SC or 25 *COD High Range Mercury Tubes *0077-SC *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
INTERFERENCES: Volatile organic compounds are not oxidized to the extent that they are in the vapor above the digestion solution. Therefore, they do not contribute to the COD reading. Contains mercury sulfate to prevent interference from chloride. Nitrite gives a positive interference of 1.1 ppm O2 per ppm NO2–N, which is insignificant unless nitrite concentrations are very high. Other reduced inorganic compounds are stoichiometrically oxidized, causing a positive interference.
PROCEDURE Use COD adapter (see p. 22). 1. Homogenize sample if necessary. 2. Preheat COD heater block to 150±2°C. 3. Remove cap from COD tube. Hold tube at a 45° angle. Use a graduated pipet, to carefully add 0.2 mL sample water allowing the sample to run down the side of the tube. 4. Cap and mix thoroughly. 5. Rinse the outside of the tube with distilled water. Wipe dry with a paper towel. 6. Repeat steps 3 through 5 using 0.2 mL distilled water. This is the reagent blank. 7.
Smart2 TEST PROCEDURES 03.
COLOR PLATINUM COBALT METHOD · NO REAGENTS REQUIRED Color in water may be attributed to humus, peat, plankton, vegetation, and natural metallic ions, such as iron and manganese, or industrial waste. Color is removed to make water suitable for domestic and industrial use. Color may have to be removed from industrial waste before it is discharged to a waterway. APPLICATION: Potable water and water with color due to natural materials.
PROCEDURE Press and hold ON burton until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 28 Color) from TESTING MENU. 5. Scroll to and select 28 Color from menu. 6. Rinse a tube (0290) with color-free water (distilled or deionized water). Fill to 10 mL line with color-free water. 7. Insert the tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter. Empty tube. 9. Rinse tube with sample water.
COPPER–LOW RANGE BICINCHONINIC ACID METHOD • CODE 3640-SC QUANTITY 50 CONTENTS *Copper Tablets CODE *T-3808-H *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. The copper content of drinking water generally falls below 0.03 parts per million, but copper levels as high as 1.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 29 Copper BCA-LR) from TESTING MENU. 5. Scroll to and select 29 Copper BCA-LR from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter and add one *Copper Tablet (T-3808).
COPPER CUPRIZONE METHOD • CODE 4023 QUANTITY CONTENTS CODE 15 mL Copper A P-6367-E 15 mL *Copper B *P-6368-E *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. The copper content of drinking water generally falls below 0.03 parts per million, but copper levels as high as 1.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 31 Cu-Cuprizone) from TESTING MENU. 5. Scroll to and select 31 Cu-Cuprizone from menu. 6. Rinse a tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert the tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter and add 5 drops of Copper A (6367). Cap and mix. 9.
COPPER DIETHYLDITHIOCARBAMATE METHOD • CODE 3646-SC QUANTITY 15 mL CONTENTS *Copper 1 CODE *6446-E *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. The copper content of drinking water generally falls below 0.03 parts per million, but copper levels as high as 1.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 32 Copper DDC) from TESTING MENU. 5. Scroll to and select 32 Copper DDC from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter and add 5 drops of *Copper 1 (6446). Cap and mix.
COPPER–UDV BICINCHONINIC ACID METHOD–UNIT DOSE VIALS CODE 4314-H QUANTITY CONTENTS 1 Copper Unit Dose Vials, 10 pouches CODE 4314-H Equipment needed but not suppled: STANDARD ACCESSORY PACKAGE · CODE 1961 1 Package of 3 Vials (empty) 0156 1 Syringe, 3 mL, plastic 1184 1 Foil Storage Bag 9467 Or: ADVANCED ACCESSORY PACKAGE · CODE 1962 1 Pipettor, 3 mL 30528 1 Pipet Tip (0-5 mL) 30695 1 Cuvette Rack 31695 1 Package of 3 Vials (empty) 0156 1 Foil Storage Bag 9467 The copper cont
Copper has a tendency to be adsorbed to the surface of the sample container. Samples should be analyzed as soon as possible after collection. If storage is necessary, 0.5 mL of 20% hydrochloric acid per 100 mL of sample will prevent “plating out”. However, a correction must be made to bring the reaction into the optimum pH range. INTERFERENCES: High concentrations of oxidizing agents, calcium, and magnesium interfere. Silver can also interfere.
PROCEDURE Use 10 mm square cell adapter. 1. Press and hold ON button until colorimeter turns on. 2. Press ENTER to start. 3. Press ENTER to select TESTING MENU. 3. Select ALL TESTS (or another sequence containing 33 Copper-UDV) from TESTING MENU. 4. Scroll to and select 33 Copper-UDV from menu. 5. Rinse a clean vial (0156) with sample water. 6. Use the syringe (1184) to add 3 mL of sample to the vial. 7. Insert the vial into chamber, close lid and select SCAN BLANK. 8. Remove vial from the colorimeter. 9.
Smart2 TEST PROCEDURES 2.
CYANIDE PYRIDINE-BARBITURIC ACID METHOD • CODE 3660-SC QUANTITY CONTENTS 60 mL Cyanide Buffer CODE 2850PS-H 5g *Cyanide Cl Reagent *2794DS-C 5g *Cyanide Indicator Reagent *2793DS-C 15 mL *Hydrochloric Acid 1N *6130-E 15 mL *Sodium Hydroxide 1N *4004-E 2 Spoons, 0.1 g, plastic 0699 1 Pipet, plastic, 1.0 mL 0354 1 pH Short Range Test Paper, pH 9–14 2955 1 Stirring Rod, Plastic 0519 *WARNING: Reagents marked with an * are considered to be potential health hazards.
PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 35 Cyanide) from TESTING MENU. Scroll to and select 35 Cyanide from menu. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. Dip the end of plastic rod (0519) into water sample and touch it to a small piece (1/4 inch) of pH test paper (2955) to wet paper.
CYANURIC ACID MELAMINE METHOD–TURBIDITY • CODE 366I-SC QUANTITY 2 x 100 mL 1 CONTENTS *Cyanuric Acid Test Solution Syringe, 5 mL CODE *4856-J 0807 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. Cyanuric acid is added to swimming pool water as a stabilizing agent for free chlorine residuals.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 36 Cyanuric) from TESTING MENU. 5. Scroll to and select 36 Cyanuric from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter and pour out water.
CYANURIC ACID–UDV MELAMINE METHOD-TURBIDITY–UNIT DOSE VIALS CODE 4313-H QUANTITY CONTENTS CODE 1 Cyanuric Acid Unit Dose Vials, 10 pouches 4313-H Equipment needed but not suppled: STANDARD ACCESSORY PACKAGE · CODE 1961 1 Package of 3 Vials (empty) 0156 1 Syringe, 3 mL, plastic 1184 1 Foil Storage Bag 9467 Or: ADVANCED ACCESSORY PACKAGE · CODE 1962 1 Pipettor, 3 mL 30528 1 Pipet Tip (0-5 mL) 30695 1 Cuvette Rack 31695 1 Package of 3 Vials (empty) 0156 1 Foil Storage Bag 9467 C
PROCEDURE Use 10 mm square cell adapter. 1. Press and hold ON button until colorimeter turns on. 2. Press ENTER to start. 3. Press ENTER to selct TESTING MENU. 4. Select ALL TESTS (or another sequence containing 37 Cyanuric-UDV) from TESTING MENU. 5. Scroll to and select 37 Cyanuric-UDV from menu. 6. Rinse a clean vial (0156) with sample water. 7. Use the syringe (1184) to add 3 mL of sample to the vial. 8. Insert the vial into chamber, close lid and select SCAN BLANK. 9. Remove vial from colorimeter. 10.
DISSOLVED OXYGEN WINKLER COLORIMETRIC METHOD • CODE 3688-SC QUANTITY CONTENTS CODE 30 mL *Manganese Sulfate Solution *4167-G 30 mL *Alkaline Potassium Iodide Azide *7166-G 30 mL *Sulfuric Acid 1:1 *6141WT-G 1 Sample Tube, screw cap 29180 1 Cap 28570 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
COLLECTION & TREATMENT OF THE WATER SAMPLE Steps 1 through 4 below describe proper sampling technique in shallow water. For sample collection at depths beyond arm’s reach, special water sampling apparatus is required (e.g. the LaMotte Water Sampling Chamber, Code 1060; Model JT-1 Water Samplers, Code 1077; Water Sampling Outfit, Code 3103; or Water Sampling Bottle, Code 3-0026). 1. To avoid contamination, thoroughly rinse the screw cap Sample Tube (29180) with sample water. 2.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 39 DO) from TESTING MENU. 5. Scroll to and select 39 DO from menu. 6. Rinse a clean tube (0290) with untreated sample water. Fill to the 10 mL line with sample. This tube is the BLANK. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Fill a second tube (0290) to the 10 line with the treated “Fixed” sample.
Smart2 TEST PROCEDURES 2.
FLUORIDE SPADNS METHOD • CODE 3647-01-SC QUANTITY CONTENTS CODE 4 x 30 mL *Acid Zirconyl SPADNS Reagent *3875-G 2 x 30 mL *Sodium Arsenite Solution *4128-G 1 Pipet, 0.5 mL, plastic 0353 1 Pipet, 1.0 mL, plastic 0354 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
The following substances produce a negative interference at the concentration given: Alkalinity (CaCO3) 5000 mg/L Aluminum (Al3+) 0.1 mg/L Iron (Fe3+) 10 mg/L Sulfate (SO4 –2) 200 mg/L Color and turbidity must be removed or compensated for in the procedure. Temperature should be maintained within 5°C of room temperature. Fluoride 2/3 Smart2 TEST PROCEDURES 2.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 41 Fluoride) from TESTING MENU. 5. Scroll to and select 41 Fluoride from menu. 6. This test requires a reagent blank. Rinse a clean tube (0290) with clear, colorless, fluoride free water. Fill to the 10 mL line with clear, colorless, fluoride free water. 7. Use the 0.5 mL pipet (0353) to add 0.5 mL of *Sodium Arsenite Solution (4128).
Smart2 TEST PROCEDURES 2.
HYDRAZINE p-DIMETHYLAMINOBENZALDEHYDE METHOD CODE 3656-SC QUANTITY CONTENTS CODE 2x60 mL *Hydrazine Reagent A *4841-H 10 g *Hydrazine Reagent B Powder *4842-D 1 Pipet, 1.0 mL, plastic 0354 1 Spoon, 0.15 g, plastic 0727 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 45 Hydrazine) from TESTING MENU. 4. Scroll to and select 45 Hydrazine from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from colorimeter. Use the 1 mL pipet (0354) to add 4 mL of *Hydrazine Reagent A (4841). Cap and mix.
HYDROGEN PEROXIDE–LOW RANGE DPD METHOD • CODE 3662-SC QUANTITY CONTENTS CODE 30 mL *Hydrogen Peroxide Reagent #1 *6452-G 100 *Hydrogen Peroxide LR Tablets *6454A-J 1 Tablet Crusher 0175 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Press and hold ON until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 46 H Peroxide-LR) from TESTING MENU. 5. Scroll to and select 46 H Peroxide-LR from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample 7. Insert tube into chamber, close lid and select SCAN BLANK. (See Note) 8. Remove tube from Colorimeter and add 4 drops of *Hydrogen Peroxide Reagent #1 (6452). Cap and mix. 9.
HYDROGEN PEROXIDE– HIGH RANGE DPD Method • CODE 4045 QUANTITY CONTENTS CODE 30 mL *Hydrogen Peroxide Reagent #1 *6452-G 100 *Hydrogen Peroxide LR Tablets *6454A-J 1 Tablet Crusher 0175 1 Pipet, glass 0342 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select Testing Menu. Select ALL TESTS (or another sequence containing 47 H Per-HR) from TESTING MENU. 5. Scroll to and select 47 H Per-HR from menu. 6. Use the pipet (0342) to add 5 drops of the sample water to a tube (0290). 7. Dilute to the 10 mL line with distilled or hydrogen peroxide-free water. 8. Insert the tube into chamber, close lid and select SCAN BLANK. 9.
HYDROGEN PEROXIDE–SHOCK DPD Method • CODE 4045 QUANTITY CONTENTS CODE 30 mL *Hydrogen Peroxide Reagent #1 *6452-G 100 *Hydrogen Peroxide LR Tablets *6454A-J 1 Tablet Crusher 0175 1 Pipet, glass 0342 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select Testing Menu. Select ALL TESTS (or another sequence containing 48 H Per Shock) from TESTING MENU. 5. Scroll to and select 48 H Per Shock from menu. 6. Use the pipet (0342) to add 5 drops of the sample water to a tube (0290). 7. Dilute to the 10 mL line with distilled or hydrogen peroxide-free water. 8. Insert the tube into chamber, close lid and select SCAN BLANK. 9.
IRON I,I0-PHENANTHROLINE METHOD • CODE 3668-SC QUANTITY CONTENTS CODE 15 mL *Acid Phenanthroline Indicator *2776-E 5g *Iron Reducing Reagent *2777-C 1 Spoon, 0.1 g, plastic 0699 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.. Most natural waters contain some iron.
PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 53 Iron Phen) from TESTING MENU. Scroll to and select 53 Iron Phen from menu. Rinse a clean tube (0290)with sample water. Fill to the 10 mL mark with sample. Insert tube into chamber, close lid and select SCAN BLANK. Remove the tube from colorimeter.
IRON BIPYRIDYL METHOD • CODE 3648-SC QUANTITY CONTENTS CODE 30 mL *Iron Reagent #1 *V-4450-G 5g *Iron Reagent #2 Powder *V-4451-C 1 Pipet, 0.5 mL, plastic 0353 1 Spoon, 0.1 g, plastic 0699 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. Most natural waters contain some iron.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 51 Iron Bipyr) from TESTING MENU. 5. Scroll to and select 51 Iron Bipyr from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter. Use the 0.5 mL pipet (0353) to add one measure of *Iron Reagent #1 (V-4450).
IRON–UDV BIPYRIDYL METHOD–UNIT DOSE VIALS · CODE 4315-H QUANTITY 1 CONTENTS *Total Iron Unit Dose Vials, 10 pouches CODE *4315-H *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
The sample container should be cleaned with acid and rinsed with deionized water. Addition of acid to adjust the sample th pH 2-3 will prevent depositation of iron on the container walls. Samples should be analyzed as soon as possible. INTERFERENCES: Strong oxidizing agents interfere, as well as copper and cobalt in excess of 5.0 ppm. SAMPLE HANDLING & PRESERVATION: Iron–UDV 2/3 Smart2 TEST PROCEDURES 2.
PROCEDURE Use 10 mm square cell adapter. 1. Press and hold ON button until colorimeter turns on. 2. Press ENTER to start. 3. Press ENTER to select TESTING MENU. 4. Select ALL TESTS (or another sequence containing 52 Iron-UDV) from TESTING MENU. 5. Scroll to and select 52 Iron-UDV from menu. 6. Rinse a clean vial (0156) with sample water. 7. Use the syringe (1184) to add 3 mL of sample to the vial. 8. Insert the vial into the chamber, close the lid and select SCAN BLANK. 9.
Smart2 TEST PROCEDURES 2.
LEAD PAR METHOD · CODE 4031 QUANTITY CONTENTS CODE 250 mL *Ammonium Chloride Buffer *4032-K 15 mL *Sodium Cyanide, 10% *6565-E 30 mL *PAR Indicator *4033-G 30 mL 15 mL Stabilizing Reagent *DDC Reagent 4022-G *4034-E 1 Syringe, 5 mL, plastic 0807 2 Pipet, 0.5 mL, plastic 0353 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com.
PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. Press and hold ON until colorimeter turns on. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 054 Lead) from TESTING MENU. Scroll to and select 054 Lead from menu. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. Insert the tube into chamber, close lid and select SCAN BLANK. Remove the tube from colorimeter. Empty the tube. Use the Syringe (0807) to add 5 mL of sample to the tube.
MANGANESE–LOW RANGE PAN METHOD • CODE 3658-SC QUANTITY CONTENTS CODE 2x60 mL *Hardness Buffer Reagent *4255-H 30 mL *Manganese Indicator Reagent *3956-G 15 mL *Sodium Cyanide, 10% *6565-E 1 Pipet, 0.5 mL, plastic 0369 1 Pipet, 1.0 mL, plastic 0354 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax..
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 55 Manganese L) from TESTING MENU. 5. Scroll to and select 55 Manganese L from menu. 6. Rinse a tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter. Use the 1.0 mL pipet (0354) to add 2.
MANGANESE–HIGH RANGE PERIODATE METHOD • CODE 3669-SC QUANTITY CONTENTS 10 g Manganese Buffer Reagent 15 g *Manganese Periodate Reagent CODE 6310-D *6311-E 1 Spoon, 0.1 g, plastic 0699 1 Spoon, 0.15 g, plastic 0727 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 56 Manganese H) from TESTING MENU. 5. Scroll to and select 56 Manganese H from menu. 6. Rinse a tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter. Use the 0.1 g spoon (0699) to add two measures of Manganese Buffer Reagent (6310).
MERCURY TMK METHOD · CODE 4861 QUANTITY CONTENTS CODE 50 *TMK Tablets *4862-H 2 x 250 mL *Propyl Alcohol *4863-K 250 mL *Acetate Buffer *4864-K 1 Tablet Crusher 0175 1 Test Tube, 10 , glass, w/cap 0778 1 Pipet, 1.0 mL, plastic 0354 1 0.5 mL, plastic 0353 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com.
PREPARATION OF *TMK INDICATOR þ NOTE: Prepare *TMK Indicator daily. Keep out of direct sunlight. 1. Fill test tube (0778) to the 10 mL line with *Propyl Alcohol (4863). 2. Add one *TMK Tablet (4862). 3. Use tablet crusher (0175) to completely crush tablet. 4. Cap and mix. Shake vigorously for 30 seconds. PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU.
MOLYBDENUM–HIGH RANGE THIOGLYCOLATE METHOD • CODE 3699-02-SC QUANTITY CONTENTS CODE 2 x 30 mL *Mo Buffer *3997-G 2 x 30 mL *Molybdenum Oxidizing Reagent *6485-G 2.5g *Molybdenum Indicator Powder *6486-S 1 Spoon, 0.05g, plastic 0696 2 Pipets, 1.0 mL, plastic w/cap 0372 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 61 Moly-HR) from TESTING MENU. 5. Scroll to and select 61 Moly-HR from menu. 6. Fill clean tube (0290) to 10 mL line with sample water. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter. Use a 1.0 mL pipet (0372) to add 1.0 mL of *Mo Buffer (3997). Cap and mix. 9. Use a second 1.
NICKEL DIMETHYLGLYOXIME METHOD • CODE 3663-SC QUANTITY CONTENTS 60 mL *Hydrochloric Acid, 2.5N 30 g *Ammonium Persulfate Reagent 30 mL *Silver Nitrate Solution, 0.0141N CODE *6251PS-H *6566-G *6346WT-G 250 mL Sodium Citrate, 10% 6253-K 60 mL *Dimethylglyoxime, 1% *6254-H 60 mL *Ammonium Hydroxide, Conc. *6537-H 3 Pipets, 1.0 mL, plastic 0354 1 Spoon, 0.1 g, plastic 0699 1 Test tube, 5-10-12.
PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. Use the 10 mL graduated cylinder (0416) to measure 10 mL of sample water. Pour into glass test tube (0608). Use the 1 mL pipet (0354) to add 1 mL of *Hydrochloric Acid, 2.5N (6251). Use the 0.1 g spoon (0699) to add 2 measures of *Ammonium Persulfate Reagent (6566). Add two drops of *Silver Nitrate Solution, 0.0141N (6346WT). Mix until the powder has dissolved. The solution will be slightly cloudy at this point.
NITRATE ZINC REDUCTION · CODE 3689-SC QUANTITY 50 1 CONTENTS *Nitrate Spectrophotometric Grade Tablets Tablet Crusher CODE *3881A-H 0175 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. Nitrogen is essential for plant growth, but excessive amounts in water supplies can result in nutrient pollution.
PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 66 Nitrate-TT) from TESTING MENU. Scroll to and select 66 Nitrate-TT from menu. Rinse a tube (0290) with sample water. Fill to 10 mL line with sample. Insert the tube into chamber, close lid and select SCAN BLANK. Remove the tube from colorimeter.
NITRATE-NITROGEN–LOW RANGE CADMIUM REDUCTION METHOD • CODE 3649-SC QUANTITY CONTENTS CODE 2 x 60 mL *Mixed Acid Reagent *V-6278-H 5g *Nitrate Reducing Reagent *V-6279-C 1 Spoon, 0.1 g, plastic 0699 1 Dispenser Cap 0692 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
INTERFERENCES: Nitrite interferes at all levels. Strong oxidizing and reducing substances interfere. Low results might be obtained for samples that contain high concentrations of iron and copper. Nitrate-Nitrogen–LR 2/3 Smart2 TEST PROCEDURES 1.
PROCEDURE þ NOTE: Place Dispenser Cap (0692) on *Mixed Acid Reagent (V-6278). Save this cap for refill reagents. 1. Press and hold ON button until colorimeter turns on. 2. Press ENTER to start. 3. Press ENTER to select TESTING MENU. 4. Select ALL TESTS (or another sequence containing 64 Nitrate-N LR) from TESTING MENU. 5. Scroll to and select 64 Nitrate-N LR from menu. 6. Rinse a clean tube (0290) with sample water. Fill to 10 mL line with sample. 7.
Smart2 TEST PROCEDURES 1.
NITRITE DIAZOTIZATION • CODE 3694-SC QUANTITY 50 1 CONTENTS *Nitrite Spectrophotometric Grade Tablets Tablet Crusher CODE *3886A-H 0175 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 69 Nitrite-TT) from TESTING MENU. Scroll to and select 69 Nitrite-TT from menu. Rinse a tube (0290) with sample water. Fill to 10 mL line with sample. Insert the tube into chamber, close lid and select SCAN BLANK. Remove the tube from colorimeter.
NITRITE-NITROGEN–LOW RANGE DIAZOTIZATION METHOD • CODE 3650-SC QUANTITY CONTENTS CODE 2 x 60 mL *Mixed Acid Reagent *V-6278-H 5g *Color Developing Reagent *V-6281-C 1 Spoon, 0.1 g, plastic 0699 1 Dispenser Cap 0692 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE þ NOTE: Place Dispenser Cap (0692) on *Mixed Acid Reagent (V-6278). Save this cap for refill reagents. 1. Press and hold ON button until colorimeter turns on. 2. Press ENTER to start. 3. Press ENTER to select TESTING MENU. 4. Select ALL TESTS (or another sequence containing 67 Nitrite-N LR) from TESTING MENU. 5. Scroll to and select 67 Nitrite-N LR from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 7.
NITROGEN, TOTAL CHROMOTROPIC ACID WITH PERSULFATE DIGESTION METHOD • CODE 4026 QUANTITY CONTENTS 25 Total Nitrogen Hydroxide Reagent Tubes 5g *Digestion Reagent Powder 60 mL Deionized Water CODE 4040-G 4036-C *5115PS-H 5g *Total Nitrogen Reagent A Powder *4041-C 30 *Total Nitrogen Reagent B Tablets *4042A-G 25 *Total Nitrogen Acid Reagent Tubes *4043-G 2 Spoon, 0.15 g, plastic 0727 4 Pipets, 1.
Nitrogen is essential for plant growth, but the presence of excessive amounts in water supplies presents a major pollution problem. Nitrogen compounds may enter water as nitrates or be converted to nitrates from agricultural fertilizers, sewage, industrial and packing house wastes, drainage from livestock feeding areas, farm manures and legumes. Nitrates in large amounts can cause “blue babies” (methemoglobinemia) in infants less than six months of age.
PROCEDURE Use COD adapter. 1. Preheat COD reactor to 100 ±2°C. Follow safety precautions. 2. Remove caps from two *Total Nitrogen Hydroxide Reagent Tubes (4040). 3. Use a 0.15 g spoon (0727) and a funnel (0459) to add one level measure of *Digestion Reagent Powder (4036) to each tube. Tap funnel to dispense powder completely. 4. Use a 1.0 mL pipet (0354) to add 2.0 mL of Deionized Water (5115PS), or organic-free water, to one tube. This is the blank. 5. Use another 1.0 mL pipet (0354) to add 2.
23. 24. 25. 26. 27. þ Note: Invert slowly and completely for accurate results. Hold tubes with caps up. Invert the tube and wait for the air bubble to flow to the bottom of the tube. Turn the tube upright and wait for the air bubble to return to the top of the tube. This is one inversion. Wait 5 minutes. Wipe the tubes with a damp towel to remove fingerprints and smudges. Wipe with a dry towel. Insert the blank tube into the chamber. Select SCAN BLANK. Remove the blank tube from the colorimeter.
OXYGEN SCAVENGERS DEHA (Diethylhydroxylamine), Carbohydrazide, Erythorbic Acid, Hydroquinone, Mehtylethylketoxime IRON REDUCTION METHOD · CODE 4857 QUANTITY CONTENTS CODE 15 mL *DEHA Reagent #1 *4791-E 15 mL *DEHA Reagent #2 *4792-E 15 mL *DEHA Reagent #3 *4793-E *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com.
DEHA PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 38 DEHA from TESTING MENU. Scroll to and select 38 DEHA from menu. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. Insert the tube into chamber, close lid and select SCAN BLANK. Remove the tube from colorimeter. Add 3 drops of *DEHA Reagent #1 (4791).
CARBOHYDRAZIDE PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 14 c-hydrazide from TESTING MENU. Scroll to and select 14 c-hydrazide from menu. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. Insert the tube into chamber, close lid and select SCAN BLANK. Remove the tube from colorimeter.
ERYTHORBIC ACID PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 40 E-thorbic A from TESTING MENU. Scroll to and select 40 E-thorbic A from menu. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. Insert the tube into chamber, close lid and select SCAN BLANK. Remove the tube from colorimeter.
HYDROQUINONE PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 49 H-quinone from TESTING MENU. Scroll to and select 49 H-quinone from menu. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. Insert the tube into chamber, close lid and select SCAN BLANK. Remove the tube from colorimeter.
METHYLETHYLKETOXIME PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 58 m-e-ketoxim from TESTING MENU. Scroll to and select 58 m-e-ketoxim from menu. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. Insert the tube into chamber, close lid and select SCAN BLANK. Remove the tube from colorimeter.
OZONE DPD METHOD • CODE 4881 QUANTITY CONTENTS CODE 30 mL DPD #1A Free Chlorine Reagent P-6740-G 30 mL *DPD #1B Free Chlorine Reagent *P-6741-G 30 mL *DPD #3 Total Chlorine Reagent *P-6743-G *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 73 Ozone DPD) from TESTING MENU. Scroll to and select 73 Ozone DPD from menu. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. Insert the tube into chamber, close lid and select SCAN BLANK. Remove tube from colorimeter. Add 5 drops *DPD #3 Total Chlorine Reagent (6743).
OZONE INDIGO METHOD • CODE 365I-SC QUANTITY CONTENTS CODE 15 mL Chlorine Inhibitor 3990-E 250 mL *Ozone Buffer *3991-K 30 mL Indigo Blue Stock Solution 3989-G 1 Sampling Apparatus 1 Pipet, transfer, 1.0 mL 1 Pipet, transfer, 5 mL 1 Pump, 10 mL 30527 1 Bottle, HR Reagent, amber glass 0680-J 1 Graduated Cylinder, 50 mL, glass 0681 2-2170 0329 0418 *WARNING: Reagents marked with an * are considered to be potential health hazards.
PROCEDURE–LOW RANGE A. PREPARATION OF HR REAGENT þ NOTE: The quantity of Indigo Blue Stock solution (3989) supplied will prepare one batch of HR Reagent for the High Range Ozone procedure or five batches of HR Reagent for the Low Range Ozone procedure. 1. Use the 50 mL graduated cylinder to carefully add 45 mL of *Ozone Buffer (3991) to amber glass bottle marked HR Reagent (0680). 2.
PROCEDURE–HIGH RANGE A. PREPARATION OF HR REAGENT þ NOTE: The quantity of Indigo Blue Stock solution (3989) supplied will prepare one batch of HR Reagent for the High Range Ozone procedure or five batches of HR Reagent for the Low Range Ozone procedure. 1. Use the 50 mL graduated cylinder to carefully add 25 mL of *Ozone Buffer (3991) to amber glass bottle marked HR Reagent (0680). 2. Use the 50 mL graduated cylinder to carefully add 25 mL of Indigo Blue Stock Solution (3989) to the amber glass bottle.
Smart2 TEST PROCEDURES 2.
pH COLORIMETRIC METHOD • CODE 3700-SC QUANTITY CONTENTS CODE 60 mL Chlorphenol Red Indicator V-2209-H 60 mL Phenol Red Indicator V-2304-H 60 mL Thymol Blue Indicator V-2213-H 3 Pipets, 0.5 mL, plastic w/caps 0369 The term pH (always written with a lower case p and an upper case H) is correctly defined as the negative logarithm of the hydrogen ion concentration.
INTERFERENCES: Sample color and turbidity interfere with the colorimetric pH measurement. Color interference may be removed bystandardizing the instrument with the original water sample. Two drops of 0.1N sodium thiosulfate per 100 mL of sample will eliminate chlorine interference. INDICATOR, RANGE, & TEST NAME: pH 2/3 pH Indicator pH Smart2 Test Name Chlorphenol Red 5.0-6.8 74 pH CPR Phenol Red 6.6-8.4 75 pH PR Thymol Blue 8.0-9.6 76 pH TB Smart2 TEST PROCEDURES 2.
PROCEDURE Use Indicator, Range, & Test Name chart to select the indicator, corresponding to anticipated pH range and to determine corresponding test name to select from colorimeter menu. 2. Press and hold ON button until colorimeter turns on. 3. Press ENTER to start. 4. Press ENTER to select TESTING MENU. 5. Select ALL TESTS (or another sequence containing the appropriate pH test name) from TESTING MENU. 6. Scroll to and select the appropriate pH test name from menu. 7.
Smart2 TEST PROCEDURES 2.
PHENOL AMINOANTIPYRINE METHOD • CODE 3652-SC QUANTITY CONTENTS 5g Aminoantipyrine Reagent CODE 7825-C 30 mL *Ammonium Hydroxide Solution *7826-G 2 x 60 mL *Potassium Ferricyanide Solution *7827-H 1 Spoon, 0.1 g, plastic 0699 1 Pipet, plain, plastic 0352 1 Pipet, 1.0 mL, plastic 0354 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 77 Phenol)from TESTING MENU. 5. Scroll to and select 77 Phenol from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter. Use the 0.1 g spoon (0699) to add one measure of Aminoantipyrine Reagent (7825-C).
PHOSPHATE–LOW RANGE ASCORBIC ACID REDUCTION METHOD • CODE 3653-SC QUANTITY CONTENTS CODE 60 mL *Phosphate Acid Reagent *V-6282-H 5g *Phosphate Reducing Reagent *V-6283-C 1 Pipet, 1 mL, plastic 0354 1 Spoon, 0.1 g, plastic 0699 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
INTERFERENCES: a. No interference from copper, iron, or silicate at concentrations many times the concentration of sea water. However, high iron concentrations can cause precipitation and subsequent loss of phosphorus. b. Salt error for samples ranging from 5% to 20% salt content was found to be less than 1%. c. Mercuric chloride, HgCl2, when used as the preservative, interferes when the chloride levels are low (less than 50 mg/L).
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 78 Phosphate L) from TESTING MENU. 5. Scroll to and select 78 Phosphate L from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter. Use 1.0 mL pipet (0354) to add 1.0 mL of *Phosphate Acid Reagent (V-6282).
Smart2 TEST PROCEDURES 2.
PHOSPHATE–HIGH RANGE VANADOMOLYBDOPHOSPHORIC ACID METHOD CODE 3655-SC QUANTITY 4 x 30 mL 1 CONTENTS *VM Phosphate Reagent Pipet, 1.0 mL, plastic CODE *4410-G 0354 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 79 Phosphate H) from TESTING MENU. 5. Scroll to and select 79 Phosphate H from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter. Use the 1.0 mL pipet (0354) to add 2.0 mL of *VM Phosphate Reagent (4410).
PHOSPHORUS, TOTAL–LOW RANGE ASCORBIC ACID REDUCTION WITH PERSULFATE DIGESTION METHOD • CODE 4024 QUANTITY CONTENTS CODE 25 *Total Phosphorus Acid Reagent Tubes *4035-G 5g *Digestion Reagent Powder *4036-C 2 X 30 mL *Total Phosphorus LR Hydroxide Reagent *4038-G 2 X 30 mL *Phosphate Acid Reagent *V-6282-G 5g Phosphate Reducing Reagent V-6283-C 1 Spoon, 0.15 g, plastic 0727 3 Pipets, 1.0 mL, plastic 0354 1 Spoon, 0.
Phosphorus in natural waters and wastewaters occurs almost exclusively in the form of orthophosphates, condensed phosphates (pyro-, meta- and other polyphosphates) and organically bound phosphates. Phosphates may be added in small amounts to water supplies during treatment. Larger amounts are introduced to water used for cleaning or laundering as components of commercial cleaning preparations. Phosphates are used to treat boiler water and are components of agricultural and residential fertilizers.
PROCEDURE Use COD adapter. 1. Preheat COD reactor to 150 ±2°C. Follow safety precautions. 2. Remove cap from a *Total Phosphorus Acid Reagent Tube (4035). Use a 1.0 mL pipet (0354) to add 5.0 mL of sample. 3. Use the 0.15 g spoon (0727) and a funnel (0459) to add one level measure of *Digestion Reagent Powder (4036). Tap funnel to dispense powder completely. Cap tube tightly and shake until powder completely dissolves. 4. Place the tube in the COD reactor for 30 minutes. 5.
Smart2 TEST PROCEDURES 11.
PHOSPHORUS, TOTAL–HIGH RANGE MOLYBDOVANADATE METHOD WITH ACID PERSULFATE DIGESTION • CODE 4025 QUANTITY 25 CONTENTS CODE *Total Phosphorus Acid Reagent Tubes 60 mL *4035-G Deionized Water 5115PS-H 5g *Digestion Reagent Powder *4036- C 2 X 30 mL *Total Phosphorus HR Hydroxide Reagent *4037-G 30 mL *Total Phosphorus HR Indicator Reagent *4039-G 1 Spoon, 0.15 g 0727 3 Pipets 1.0 mL, plastic 0354 1 Pipet, 0.
polyphosphates) and organically bound phosphates. Phosphates may be added in small amounts to water supplies during treatment. Larger amounts are introduced to water used for cleaning or laundering, as components of commercial cleaning preparations. Phosphates are used to treat boiler water and are components of agricultural and residential fertilizers. Phosphorus is an important nutrient for aquatic plants. The amount found in natural water is generally not more than 0.
PROCEDURE Use COD adapter. 1. Preheat COD reactor to 150 ±2°C. Follow safety precautions. 2. Remove cap from a *Total Phosphorus Acid Reagent Tube (4035). Use a 1.0 mL pipet (0354) to add 5.0 mL of Deionized Water (5115PS). This is the blank. 3. Remove cap from a *Total Phosphorus Acid Reagent Tube (4035). Use the 1.0 mL pipet (0354) to add 5.0 mL of sample water. This is the sample. 4. Use the 0.15 g spoon (0727) and a funnel (0459) to add one level measure of *Digestion Reagent Powder (4036) to each tube.
Smart2 TEST PROCEDURES 2.
POTASSIUM TETRAPHENYLBORON METHOD • CODE 3639-SC QUANTITY CONTENTS 30 mL *Sodium Hydroxide, 1.0N 5g *Tetraphenylboron Powder 1 Spoon, 0.05 g, plastic CODE *4004WT-G *6364-C 0696 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 81 Potassium) from TESTING MENU. 5. Scroll to and select 81 Potassium from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter. Add 4 drops of *Sodium Hydroxide, 1.0N (4004WT). Cap and mix. 9. Use the 0.
SILICA–LOW RANGE HETEROPOLY BLUE METHOD • CODE 3664-SC QUANTITY CONTENTS CODE 30 mL *Silica Reagent #1 *V-4466-G 30 mL *Silica Reagent #2 *V-4467-G 30 mL *Silica Reagent #3 *V-4468-G 10 g *Silica Reagent #4 *V-6284-D 1 Spoon, 0.1 g, plastic 0699 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
INTERFERENCES: Sulfides and large amounts of iron interfere. Color and turbidity may be removed by standardizing the instrument with the original water sample. Since silica is a component of glass waste and a common contaminant, it is suggested to run a reagent blank using silica-free water. The blank value is subtracted from the sample concentrations. Silica–LR 2/3 Smart2 TEST PROCEDURES 2.
PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 85 Silica Lo) from TESTING MENU. Scroll to and select 85 Silica Lo from menu. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. Insert tube into chamber, close lid and select SCAN BLANK. (See Note) Remove tube from colorimeter.
Smart2 TEST PROCEDURES 2.
SILICA–HIGH RANGE SILICOMOLYBDATE METHOD • CODE 3687-SC QUANTITY CONTENTS CODE 30 mL *Silica Reagent #1 *V-4466-G 30 mL *Silica Reagent #2 *V-4467-G 15 mL *Silica Reagent #3 *V-4468-G *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 86 Silica Hi) from TESTING MENU. 5. Scroll to and select 86 Silica Hi from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter. Add 6 drops *Silica Reagent #1 (V-4466). Cap and invert to mix. 9.
SULFATE–HIGH RANGE BARIUM CHLORIDE METHOD • CODE 3665-SC QUANTITY 10 g 1 CONTENTS *Sulfate Reagent Spoon, 0.1 g, plastic CODE *V-6277-D 0699 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 89 Sulfate-HR) from TESTING MENU. 5. Scroll to and select 89 Sulfate-HR from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter. Use the 0.1 g spoon (0699) to add one measure of *Sulfate Reagent (V-6277).
SULFIDE–LOW RANGE METHYLENE BLUE METHOD • CODE 3654-01-SC QUANTITY CONTENTS CODE 2 x 30 *Sulfide Reagent A *V-4458-G 15 mL *Sulfide Reagent B *V-4459-E 2 x 60 mL Sulfide Reagent C 4460-H 2 Pipets, 1.0 mL, plastic 0354 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 90 Sulfide-LR) from TESTING MENU. 5. Scroll to and select 90 Sulfide-LR from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter. Use the 1.0 mL pipet (0354) to add 1.0 mL of *Sulfide Reagent A (V-4458).
SURFACTANTS ION PAIR EXTRACTION–BROMPHENOL BLUE INDICATOR CODE 4876 QUANTITY CONTENTS 50 g pH Adjustment Powder 10 g Sodium Chloride Reagent 2 X 60 mL *DS Indicator Reagent CODE 4509- H 4877-D *4508-H 1 Spoon, 0.5 g, plastic 0698 1 Spoon, 0.1 g, plastic 0699 1 Pipet, 1.0 mL, plastic 0354 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com.
PROCEDURE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 94 Surfactants) from TESTING MENU. Scroll to and select 94 Surfactants from menu. Rinse a tube (0290) with sample water. Fill to 10 mL line with sample. Insert the tube into chamber, close lid and select SCAN BLANK. Remove the tube from colorimeter. Use the 0.5 g spoon (0698) to add 0.
TANNIN TUNGSTO-MOLYBDOPHOSPHORIC ACID METHOD CODE 3666-SC QUANTITY CONTENTS CODE 30 mL *Tannin Reagent #1 *7833-G 2 x 60 mL *Tannin Reagent #2 *7834-H 1 Pipet, plain, plastic 0352 1 Pipet, 1.0 mL, plastic 0354 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax..
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 96 Tannin) from TESTING MENU. 5. Scroll to and select 96 Tannin from menu. 6. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from colorimeter. Use the plain pipet (0352) to add 4 drops of *Tannin Reagent #1 (7833). Cap and mix. 9.
TURBIDITY ABSORPTION METHOD • NO REAGENTS REQUIRED Turbidity is a measure of water clarity and is independent of color. Turbidity is caused by undissolved and suspended solids. Mud, silt, algae, and microorganisms can all cause turbidity. Turbidity is a gross measurement of water quality. APPLICATION: Surface and industrial water for non-compliance monitoring. (For compliance monitoring at low turbidity levels, use a commercial nephelometer.
PROCEDURE Press and hold ON button until colorimeter turns on. Press ENTER to start. Press ENTER to select TESTING MENU. Select ALL TESTS (or another sequence containing 98 Turbidity) from TESTING MENU. 5. Scroll to and select 98 Turbidity from menu. 6. Rinse a clean tube (0290) with deionized water (turbidity free). Fill to the 10 mL line with deionized water. 7. Insert tube into chamber, close lid and select SCAN BLANK. 8. Rinse a second clean tube (0290) with sample water.
ZINC–LOW RANGE ZINCON METHOD • CODE 3667-SC QUANTITY CONTENTS 30 mL *Zinc Indicator Solution 120 mL *Methyl Alcohol 10 g Sodium Ascorbate Powder CODE *6314-G *6319-J 6316-D 25 g *Zinc Buffer Powder *6315-G 15 mL *Sodium Cyanide, 10% *6565-E 30 mL *Formaldehyde Solution, 37% *5128-G 1 “Dilute Zinc Indicator Solution” Bottle, w/1 pipet assembly 0128-MT 1 Graduated Cylinder, 10 mL, glass 0416 1 Spoon, 0.5 g, plastic 0698 2 Pipets, plain, plastic 0352 1 Spoon, 0.
INTERFERENCES: The following ions interfere in concentrations greater than those listed. Zinc–LR 2/3 Ion mg/L Ion mg/L Cd(II) 1 Cr(III) 10 Al (III) 5 Ni(II) 20 Mn (II) 5 Co (II) 30 Fe (III) 7 CrO4(II) 50 Fe (II) 9 Smart2 TEST PROCEDURES 2.
PROCEDURE A. 1. 2. B. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. PREPARATION OF DILUTE ZINC INDICATOR SOLUTION Use a pipet (0352) to measure exactly 5.0 mL of *Zinc Indicator Solution (6314) into 10 mL graduated cylinder (0416). The bottom of the curved surface (the meniscus) of liquid should be at 5.0 mL mark. Pour this into the bottle labeled “Dilute Zinc Indicator Solution”. Use unrinsed graduated cylinder to add 10.0 mL and then 7.8 mL (total of 17.
Smart2 TEST PROCEDURES 2.
SMART 2 ••••••••••••••••••••••••••••••••••••••••••••••• Colorimeter APPENDIX Smart2 TEST PROCEDURES 2.
Smart2 TEST PROCEDURES 2.
APPENDIX Ammonia in water occurs in two forms: toxic unionized ammonia (NH3) and the relatively non-toxic ionized form, ammonium ion (NH4+). This test method measures both forms as ammonia-nitrogen (NH3+–N) to give the total ammonia-nitrogen concentration in water. The actual proportion of each compound depends on temperature, salinity, and pH. A greater concentration of unionized ammonia is present when the pH value and salinity increase. 1.
FOR EXAMPLE: If a fresh water sample at 20°C has a pH of 8.5 and the test result is 1.0 ppm as Total Ammonia-Nitrogen: 1. The percentage from the table is 11.18% (or 0.1118). 2. 1 ppm Total Ammonia-Nitrogen x 0.1118 = 0.1118 ppm Unionized Ammonia-Nitrogen. 3. Total Ammonia-Nitrogen 1.0000 ppm Unionized Ammonia-Nitrogen - 0.1118 ppm Ionized Ammonia-Nitrogen Appendix 2/2 = 0.8882 ppm Smart2 TEST PROCEDURES 2.
