Instruction Manual

Instruction Manual | 3
A pipet that has been correctly attached and sealed in the holder should not tilt to
the sides. After attaching a pipet, hold the pipet controller in a vertical position. It
is not recommended to leave the device with a pipet attached for a long period, for
example overnight or over a weekend.
CAUTION: Do not put aside the pipet controller if there is liquid in the pipet.
Filling the pipet
Before aspirating is started, set the speed by pressing the SPEED switch (Figure 1C)
until the display shows the correct speed (Figure 1E).
 HIGH speed: fast aspirating (Figure 2.1)
 LOW speed: slow aspirating (Figure 2.2)
It is recommended to set the LOW speed when working with pipets of volumes up to
5 mL, and the HIGH speed for pipets of volumes greater than 5 mL. Holding the pipet
controller in a vertical position, immerse the pipet end in the liquid to be drawn up
(Figure 3.2), and press the aspiration button gently. The speed depends on how deep
the aspirating button has been pressed. The deeper the button is pressed the faster
the liquid is aspirated into the pipet.
It is recommended to draw a slightly greater liquid volume than required (due to
meniscus above the required volume mark), adjusting the aspiration speed, so as not
to overll the pipet.
Setting the volume
After the pipet is lled, dry the outside surface with absorbent paper that does not
leave impurities. Then set the required liquid volume precisely. Pressing the dispense
button gently (Figure 3.3), dispense the excessive liquid from the pipet until the
meniscus of the liquid aligns exactly with the required volume mark on the pipet.
Emptying the pipet
Holding the vessel in an inclined position, place the pipet end in contact with the
vessel wall and press the dispense button gently (Figure 3.3). The dispensing intensity
may be adjusted depending on how deep the dispense button has been pressed. The
deeper the button is pressed the faster the outow of liquid from the pipet.
The pipet controller has two dispense modes. The dispense mode is selected by
successive pressing the MODE switch (Figure 1D) until the display shows the correct
mode (Figure 1E).
 Gravity mode is marked with the icon on the display (Figure 2.4). Dispensing is
affected in gravity mode, which means that the liquid ows out of the pipet by its
own weight.
 Blow out mode is marked with the icon on the display (Figure 2.5). Dispensing is
affected in gravity mode. However, when the dispense button is pressed to the middle
position, the pump is started and fast emptying of the pipet with a blow out is affected.
CAUTION: During gravimetrical dispensing, the pipet is not completely emptied due
to the characteristics of pipets used with the pipet controller.