BPM/08/2004 v06 31/07/2004 01:06 PM MULTICHANNEL Labnet International, Inc.
BPM/08/2004 v06 31/07/2004 MULTICHANNEL A A1 A2 C B F D H E 01:06 PM Page 2
BPM/08/2004 v06 31/07/2004 01:06 PM Page 4 MULTICHANNEL 4A 4B G 4C 4D
BPM/08/2004 v06 31/07/2004 01:06 PM Page 6 MULTICHANNEL CONTENTS 1 - INTRODUCTION 2 - VOLUME SETTING 3 - METHOD OF PIPETTING 4 - RECOMMENDATIONS 5 - RECALIBRATION 6 - CLEANING AND STERYLIZATION 7 - PIPETTE KIT AND ACCESSORIES 8 - SPARE PARTS The specifications for accuracy and precision given in the following table are obtained using Labnet tips. These figures are only guaranteed when Labnet tips are used. BioPette 8 channel Model SPECIFICATIONS Volume Accuracy [µl] [%] Min 1 5 10 ±8.0 ±4.0 ±2.
BPM/08/2004 v06 31/07/2004 01:06 PM Page 8 MULTICHANNEL the procedures outlined in the EN ISO 8655 standard. The pipette design enables the user to perform the recalibration process according to the rules presented in section 5. If the knob is accidentally turned too far, ie is set lower than the required value, the process must be repeated. The approach to the set volume must always be made in the order of decreasing value. 2 - SETTING THE VOLUME Volume is set using the pipetting pushbutton (Fig.
BPM/08/2004 v06 31/07/2004 01:06 PM Page 10 MULTICHANNEL 4 - RECOMMENDATIONS To achieve maximum safety, precision and reliability, the following principles should be observed: • do not draw liquids without tips fitted on the pipette cones, • do not lay down the pipette with tips filled, • do not draw volumes of liquid exceeding the pipette’s range, • check if the tips are fitted properly, • during operation, the pipette must be held vertically, tips should be immersed in liquid to the depth of 2 to 4 m
BPM/08/2004 v06 31/07/2004 01:06 PM Page 12 MULTICHANNEL • Perform three aspiration series (each series should include the aspirations from all channels), weigh each time and calculate the average value of the aspirations. • Calculate average aspirated volume in µl multiplying the average aspiration amount [mg] by the distilled water density coefficient [µl/mg], which depends on temperature and pressure according to the following table: Temperature [°C] Pressure [kPa] 95 101.3 105 20 1.0028 1.
BPM/08/2004 v06 31/07/2004 01:06 PM Page 14 MULTICHANNEL Accessories Pipettor Tip Choices BPE-10 P 4803-10, 10 µl tips, racked BPE-50 BPE-200 BPT-50 BPT-200 P 4803-200, 200 µl tips, racked BPE-300 BPT-300 P 4803-300, 300 µl tips, racked Pipettor Stand Choices BPE P 5421 Stand for eight channel pipette BPT P 5422 Stand for twelve channel pipette 8 - SPARE PARTS The spare parts for multichannel, (Fig.