IN STRUC TION MANUA L Laboratory Equipment Pty Ltd email: sales@labec.com.au Ph: 02 9560 2811 • Fax: 02 9560 6131 www.labec.com.
INSTRUCTION MANUAL FOR SPECTRO- UV/V-16/18 UV/VIS SPECTROPHOTOMETERS PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION 3, Hagavish st. Israel 58817 Tel: 972 3 5595252, Fax: 972 3 5594529 mrc@mrclab.com MRC.VER.02-11.
Co ontents Safety ….…………………………………………………………………………. 1 General ………………………………………………………………………. 1 Electrical …………………………………………………………………….. 1 Warning ………………………………………………………………………… 1 Working Principle ………………………………………………………………2 Unpacking Instructions Specifications Installation …………………………………………………… 2 ……………………………………………..……………… 2 …………………………………………………………………… 3 Operation ………………………………………………………..
16/18 Manual Safety: The safety statements in this manual comply with the requirements of the HEALTH AND SAFETY AT WORK ACT, 1974. Read the following before installing and using the instrument and its accessories. The 63(&752 UV16/18 should be operated by appropriate laboratory technicians. General: The apparatus described in this manual is designed to be used by properly trained personnel in a suitable equipped laboratory.
16/18 Manual Intentional interruption is prohibited. Whenever it is likely that the protection has been impaired, the apparatus shall be made inoperative and be secured against any unintended operation. NEVER touch or handle the power supply on 63(&752 UV-16/18 due to the high voltage.
16/18 Manual Packing List Description Quantity Spectrophotometer ............................................................... 1 Mains Lead ........................................................................... 1 Cuvettes................................................................................ Set of 4, glass .......................................................................... Set of 2, quartz Manual ............................................................................
16/18 Manual your instrument, keep it as far as possible from any strong magnetic or electrical fields or any electrical device that may generate high-frequency fields. Set the unit up in an area that is free of dust, corrosive gases and strong vibrations. LCD Control Panel Compartment Holder Changer Knob Fig.1-3 3. Remove any obstructions or materials that could hinder the flow of air under and around the instrument. 4. Use the appropriate power cord and plug into a grounded outlet.
16/18 Manual Operation: Prepare the spectrophotometer Fig 1-2 is the control panel. User can perform all operations by pressing the keys and all the results and operation information are displayed on the LCD.
16/18 Manual Turn on spectrophotometer by pressing the Power Switch (IO). The instrument starts to initiate and the steps are as below: 1.The instrument will position filter first, then initialize lamp position and AD converter, then D2 / W lamps warm up, then Wavelength reset and get dark current, After initializing printer, checking battery and setting up system, the screen display the main menu. And the self-testing process display as fig 3. 2 .
16/18 Manual Fig 4 Use numeric keypad to input wavelength (e.g. 500nm). Press 【ENTER】 to change the wavelength, and then blank; After blanking, the screen displays as the left one of Fig 5. Note:1. If you don’t want to change the wavelength, you can press 【RETURN】 】. 2. the wavelength range is 190-1100, other figure is invalid. START Basic Mode： ： 0.000 Abs 500.0 nm RETURN 500.0nm No. WL 1 500.0 0.000Abs Abs 0 .000 Fig.
16/18 Manual Analyze Sample We provide three test methods in UV16/18 series, they are basic mode test, quantitative and kinetics. 1. Basic Mode Push the blank cuvette into the light path. In main menu (the last one of Fig. 3), move the cursor on “Basic mode”, then press 【ENTER】to go into basic mode test. After automatically blanking, it will display as the left one of Fig. 4 and wait for the operator. 【RETURN】to exit. ☺ Select Test Mode There are three test modes (T%, Abs, Conc.
16/18 Manual ① 5 groups of test data can display per screen, total 200 groups of data can be saved. ② In the right one of Fig. 8, you can change your test wavelength, blank, change test mode and print by press 【GOTO GOTO λ】, 【ZERO ZERO】, SET】 PRINT】respectively. ZERO 【SET SET and 【PRINT PRINT You can also clear the test data by press 【CLEAR CLEAR】. CLEAR 2. Quantitative Mode It includes two test methods, one is Standard curve, the other is Coefficient method.
16/18 Manual Standard Curve 0.000 Abs 0.000 ml/l 546.0 nm GOTO λ RETURN Standard Curve WL＝ ＝546.0nm ： Please Input New： WL= Fig. 11 ☺ Blank Pull the blank solution in the light path, then press 【ZERO】. ☺ Set parameters Press 【SET】in the pre-testing interface. It goes into parameters setting interface. (Fig.12). You should set Unit, the Number of standard samples, Concentration of standard samples before testing. Standard Curve 0.000 Abs 0.000 ml/l 546.
16/18 Manual √Standard Curve ○Unit ●Number ○Concentration ENTER RETURN Standard Curve N=2 Please Input New： ： N= Fig. 14 Input the number of your standard samples by numeric keypad. After pressing【ENTER】,it returns to the setting interface. Note：the number range is 1-9，other number is invalid. Set Concentration of Standards Before this step, you must blank first. Pull the blank cuvette in the light path in the pre-testing interface, press 【ZERO】.
16/18 Manual 1 √Standard Curve ○Concentration ●Display Curve ○Select Curve 0.500A ENTER 2 0 r=0.999 C=1.001*A+0.2 RETURN 0.8C 3 Fig. 16 1、Standard Curve 2、Relative Coefficient. 3、Curve Equation C=1.001*A+0.2, C: Concentration A: Value of Abs. The slop value of the curve is 1.001. The intercept of the curve is 0.2. If the system gives a fault hint, check the standard sample and your inputting, press any key to return and repeat your performance. (Fig.
/18 Manual to go back to pre-testing interface. Note: If you want to delete a equation, move the cursor on it, just press 【CLEAR】. ☺ Test and Print 【START/STOP】to go into testing When you have selected the equation, press interface in pre-testing interface.(Fig. 19) START Standard Curve： ： 0.000 Abs 0.000 ml/l 546.0 nm 546.0nm -0.000Abs No. WL Abs Conc 1 RETURN 546.0 0.001 1.0 g/l Fig.
16/18 Manual 1↙ Coefficient: A=KC+B 0.001 Abs 1.200 mg/l 546.0 nm Coefficient 1. A=KC+B 2. C=KA+B √Quantitative： ： ○Standard Curve +B ●Coefficient 2↙ RETURN RETURN Coefficient: C=KA+B 0.001 Abs 1.000 mg/l 546.0 nm Fig. 22 Note: The two formulas can be converted each other, it’s up to your habit to choose anyone of them, but the operation is the same. C=KA+B is an example. ☺ Set Wavelength Press【GOTO λ】in the pre-testing interface, input the testing wavelength by pressing the numeric keypad. (Fig.
16/18 Manual by【ENTER】.(Fig. 25) √Coefficient ●Coefficient K ○Coefficient B ○Conc. Unit ENTER RETURN Coefficient K=4.000 Please Input New K= Fig. 25 Input the new value of K by pressing the numeric keypad followed by 【ENTER】, it will return the setting interface. Note: the range of K is 0-9999, other value is invalid. Set Coefficient B Move the cursor on “Coefficient B” by pressing【∧ ∧】and 【∨ ∨】, followed by 【ENTER】.(Fig. 26) √Coefficient ○Coefficient K ●Coefficient B ○Conc.
16/18 Manual ☺ Test and Print Pull the unknown concentration sample in the light path, then press 【START】, now it enters the testing interface. Press 【START】again, you’ll get the concentration of the sample.(Fig. 28) Coefficient: A=KC+B 0.001 Abs 1.200 mg/l 546.0 nm 546.0nm -0.000Abs No. WL Abs Conc START 1 546.0 0.001 1.0 g/l RETURN Fig. 28 The operation is the same if you have more samples to test. Press 【PRINT】to print the test results. All the data will be deleted after printing.
16/18 Manual Press 【SET】in the kinetics pre-testing interface, 5 parameters should be set before testing.(Fig. 31) Kinetics SET 100.0 T% 546.0 nm RETURN √ Kinetics ●Time Interval ○Total Time ○Test Mode √ Kinetics ●Upper Limit ○Lower Limit ○Display Data Fig. 31 Set Time Interval Move the cursor on “Time Interval” by pressing【∧ ∧】and 【∨ ∨】, followed by【ENTER】. (Fig.32) √ Kinetics ●Time Interval ○Total Time ○Test Mode ENTER RETURN Kinetics Time Interval = 1 S Please Input New： ： T= Fig.
16/18 Manual 【ENTER】, it will return to the kinetics setting interface. Note: 1. The range of total test time is 1-120,000, other value is invalid. 2.The maximum value of total time is correlative to the interval time, because system only permits 1000 dots be adopted at most.
16/18 Manual If you don’t want to change the value being set last time, press 【ENTER】 or【RETURN】directly . Note: the range or A is -0.3-3, the range or T is 0-200, other value is invalid. Set Lower Limit Move the cursor on “Lower Limit” by pressing【∧ ∧】and 【∨ ∨】in setting interface, followed by【ENTER】(Fig.36) √ Kinetics ○Upper Limit ●Lower Limit ○Display Data ENTER RETURN Kinetics: Abs. Lower=-0.500 Please Input New Lower= Fig.
16/18 Manual interface. Display Data ∧】and 【∨ Move the cursor on “Display Data” by pressing【∧ ∨】in setting interface, followed by【ENTER】.(Fig. 38) √ Kinetics ○Upper Limit ○Lower Limit ●Display Data No. 1 2 3 4 ENTER RETURN Abs 0.000 0.001 0.002 0.003 Fig. 38 5 lines of data be displayed every screen. press【∧ ∧】and 【∨ ∨】to scroll. ☺ Data Print Press 【PRINT】in the right interface of Fig.38, the data will be printed. After printing, the data will be deleted from the screen and RAM.
16/18 Manual √Utility ●Date & Time ○Dark Current ○Reset WL. √Utility ●Lamp Life ○Load Default ○Lamp Change √Utility ●Version Fig. 40 Set Deuterium Lamp ∧】and 【∨ Move the cursor on “D2 Lamp on/off” by pressing【∧ ∨】in utility setting interface, then press【ENTER】to go into D2 controlling interface.(Fig. 41) √Utility ● D2 Lamp on/off ○ W Lamp on/off ○ Printer ENTER RETURN √D2 Lamp ●ON ○OFF √ Fig. 41 Move the cursor on “OFF” to shut off the D2 lamp by pressing【∧ ∧】and 【∨ ∨】 followed by 【ENTER】.
16/18 Manual interface.(Fig. 43) √Utility ○D2 Lamp on/off ●W Lamp on/off ○Printer ENTER RETURN √W Lamp ●ON ○OFF √ Fig. 43 Move the cursor on “OFF” to shut off the W lamp by pressing【∧ ∧】and 【∨ ∨】 followed by 【ENTER】. The icon ”√ √” also displays at the end of “OFF” . Press 【RETURN】to exit. ∧】and 【∨ Move the cursor on “On” to lighten the W lamp by pressing【∧ ∨】 followed by 【ENTER】. The icon ”√ √” also displays at the end of “ON” Press 【RETURN】to exit.
16/18 Manual ∧】and 【∨ Move the cursor on “Scroll Paper” by pressing【∧ ∨】in printer setting interface followed by 【ENTER】. Repeat the operation, till the new changed paper spread out. ∧】and 【∨ Move the cursor on “Reset” by pressing【∧ ∨】in printer setting interface followed by 【ENTER】, then the printer will be reset. If you want to check your printer, Move the cursor on “Reset” by pressing 【∧ ∧】and 【∨ ∨】in printer setting interface, then press【ENTER】, the printing content is as follows.(Fig.
16/18 Manual Fig. 48 It returns to the utility setting interface when the dark current got finished. Reset Wavelength When you are suspicious of your wavelength, you can reset your wavelength. Move the cursor on “Reset WL.” by pressing【∧ ∧】and 【∨ ∨】in utility setting interface, then press【ENTER】to reset wavelength. (Fig. 49) The calibrating time is about 1.5 minutes. √Utility ○Date & Time ○Dark Current ● Reset WL. ENTER …Calibrating WL… Auto return Fig.
16/18 Manual When you have replaced a new lamp, you’ll have to go into the lamp life setting interface, move the cursor on the “Reset W Life” or “Reset D2 Life” by ∧】and 【∨ pressing 【∧ ∨】followed by【ENTER】to reset them into zero. Load Default ∧】and 【∨ Move the cursor on “Load Default” by pressing【∧ ∨】in utility setting interface, then press【ENTER】(Fig. 52) √Utility ○Lamp Life ●Load Default ○Lamp Change ENTER Load Default？ ？ ●Yes ○No RETURN Fig.
16/18 Manual Maintenance Fan Inside Structure Lamp Chamber Radiator Compartment Mono-chromator Chamber Cell-holder Fig.4-1 Inside Planform 1. Lamp Replacement A. Replace Tungsten-Halogen Lamp Turn off and unplug the instrument. Remove the four screws on the sides of the spectrophotometer. Remove the Cuvette Holder Control Knob by unscrewing the rod counterclockwise. Remove the cover of the instrument very carefully and place it in the right of the instrument.
16/18 Manual CAUTION: 1) do not handle the lamp with bare fingers. Use tissue or cloth when handling lamp. 2) There’s no difference in polarity of the two legs of Tungsten-halogen lamp. Switch the instrument on. Set the wavelength at 340 nm, insert an empty cuvette, and blank the instrument. If the energy is low, adjust the lamp by “pulling” or “pushing” it so that the light beam is focused on the entrance slot of the mono-chromator.
16/18 Manual Fig.4-3 8. Replace a new pre-aligned lamp provided by or an authorized Service Provider. Be sure the socket of the lamp flange matches with the positioning bolt. Then tighten the two screws. 9. Reconnect the wire connector (make sure the wire connection orientation is the same as step 6.) 10. Turn on the instrument. After self-testing, select 300 nm and press【ZERO】. Check to make sure that the light beam is focused on the entrance slit of the monochromator.
16/18 Manual Troubleshooting Possible Cause PROBLEM Instrument Inoperative after switch power on Instrument cannot set 100%T (0.
