User’s Manual Protector® Controlled Atmosphere Glove Boxes Base Catalog Model Number 50600 50601 Operation Type Manual Manual 50800 50801 Auto Auto 50800 50801 Combination Combination Notes: Liner Material Fiberglass Stainless Steel Fiberglass Stainless Steel Fiberglass Stainless Steel Liner Width Single Single Double Single Single Double Single Single Double (Last Two Digits of Configured Catalog Number) Electrical Receptacle Voltage/Frequency North British China North America (UK) Schuko Australi
Copyright © 2011 Labconco Corporation. All rights reserved. The information contained in this manual and the accompanying products are copyrighted and all rights reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change. Warranty Labconco provides a warranty on all parts and factory workmanship.
TABLE OF CONTENTS CHAPTER 1: INTRODUCTION 1 CHAPTER 2: PREREQUISITES Support, Vibration & Preventive Requirements Location Requirements Oil & Exhaust Requirements for the Pressure Relief Bubbler Electrical Requirements Plumbing Requirements for Inert Gas, Vacuum, & Gas Purifier/ Drying Train Space Requirements 7 8 8 8 9 9 10 CHAPTER 3: GETTING STARTED Unpacking the Glove Box Installing the Glove Box on a Supporting Structure & Work Surface Adding the Oil & Connecting the Pressure Relief Bubbler to Outsi
Transfer Chamber Evacuation (Purge) and Fill – Operation Mode 3 Setup Menu – Operation Mode 4 39 40 CHAPTER 6: MAINTAINING YOUR GLOVE BOX Routine Maintenance Schedule Initial Certification Recertification Fluorescent Light Replacement Solenoid Valve Replacement Auto Pressure Controller Circuit Board Replacement 45 45 46 46 46 48 48 CHAPTER 7: ACCESSORIZING YOUR GLOVE BOX 50 CHAPTER 8: TROUBLESHOOTING 57 APPENDIX A: REPLACEMENT PARTS 58 APPENDIX B: DIMENSIONS 61 APPENDIX C: SPECIFICATIONS 62 AP
Chapter 1: Introduction Congratulations on your purchase of a Labconco Protector® Controlled Atmosphere Glove Box manufactured with fiberglass or stainless steel in a single or double width configuration. The Protector Controlled Atmosphere Glove Box comes equipped with four manual valves, pressure relief bubbler and either analog chamber pressure gauge indicators, or digital pressure gauge indicators.
Chapter 1: Introduction Glove Box Type Gas Permeant Glove Port Covers Protector (FRP) Protector (stainless) 0.4 0.2 O2 Installed Purity H 2O Installed 2 1 (ppm) O2 None 0.9 0.8 H 2O None 3 2 Oxygen Permeation at -4" w.g. per ISO 10648-2 Test Method (ppm/min) O2 None 0.30 0.16 ISO 10648-2 Acceptance Level for Class 1 Controlled Atmosphere Glove Box (ppm/min) O2 None <1.67 <1.67 O2 None 0.16 0.14 H 2O None 0.17 0.
Chapter 1: Introduction Protector Controlled Atmosphere Glove Box with Auto Pressure Control shown with AtmosPure Re-Gen Gas Purifier and Adjustable Height Base Stand Figure 1-1 Product Service 1-800-522-7658 3
Chapter 1: Introduction Protector Controlled Atmosphere Glove Box with Manual Pressure Control and Adjustable Height Base Stand Figure 1-2 4 Product Service 1-800-522-7658
Chapter 1: Introduction Protector Combination Glove Box utilizes Auto Pressure Control for controlled atmospheres and filtered air with Integral Blower for particulate containment and cleanliness applications. The Glove Box is shown with the Adjustable Height Base Stand.
Chapter 1: Introduction Protector Double Width Controlled Atmosphere Glove Box with Auto Pressure Control shown with AtmosPure Re-Gen Gas Purifier mounted on a doublewide mobile base stand Figure 1-4 6 Product Service 1-800-522-7658
Chapter 2: Prerequisites Before you install the glove box, you need to prepare your site for installation. You must be certain that the area is level and of solid construction. In addition, a dedicated source of electrical power should be located near the installation site to power the glove box. Additionally, the glove box should be strategically placed in the lab to provide efficient workflow and prevent operator interference from normal traffic flow.
Chapter 2: Prerequisites Support, Vibration and Preventive Requirements In the preparation of a glove box site, please consider the following: • A bench or stand that is rigidly mounted to the floor or fixed to the wall, but not both, may be appropriate. 35" to 40" (889mm to 1,016mm) is typical for standing height (Labconco adjustable height base stands offered in Chapter 7 vary from 33" to 40" or 838mm to 1,016mm).
Chapter 2: Prerequisites ! WARNING: In the case of failure caused by excessive vacuum within the main chamber, the user must safely determine whether laboratory atmosphere entering the glove box could result in fire, explosive hazard or damage to property inside the glove box. Electrical Requirements Electrical receptacles should be located nearby for connecting the glove box cords, or other optional laboratory equipment.
Chapter 2: Prerequisites All solenoid valves utilize 3/8" (9.5mm) compression fittings or 3/8" (9.5mm) hose barbs for soft tubing. Both styles are offered for your convenience. Accessory gas and vacuum tubing kits are described in Chapter 7. Should you later require automatic chamber pressure controls (only for manually operated Protector Controlled Atmosphere Glove Boxes), see Chapter 3 for details on the accessory Pressure Controller and Chapter 7 for ordering information.
Chapter 3: Getting Started Once the site for your glove box is properly prepared, you are ready to unpack, inspect, install, and validate performance of your system. Read this chapter to learn how to: • Unpack and move the glove box. • Set up the glove box with the proper supporting structure and work surface. • Add the oil and if required connect an exhaust system to the pressure relief bubbler. • Install the gloves. • Connect the electrical supply.
Chapter 3: Getting Started Unpacking the Glove Box We recommend that you do not remove the glove box from its shipping container until it is ready to be placed into its final location. Move the unit by placing a flat, low dolly under the shipping skid, or by using a floor jack. Carefully remove the shrink-wrap and crating on the glove box and inspect it for damage that may have occurred in transit.
Chapter 3: Getting Started Adding the Oil and Connecting the Pressure Relief Bubbler to Outside Exhaust The glove box ships with a separate oil container with a specific gravity of 0.87. The pressure relief bubbler prevents over or under pressurization of the glove box main chamber and activates at pressures and vacuums above +5" water gauge (1245 Pa) or below -5" water gauge (-1245 Pa) to prevent glass breakage and glove box liner damage.
Chapter 3: Getting Started Consult with Safety Officer for exhausting 3/4" OD to the outside. Fill with 0.87 specific gravity oil using a 3/4" I.D. hose and funnel. Liquid level sight gauge. Fill oil to 6" (152 mm) from bottom.
Chapter 3: Getting Started C.A. COM Port. 6-Pin Communication Cable Connector for shared vacuum pump use with accessory AtmosPure Re-Gen Gas Purifier (auto pressure control models only) Input power cord number one ON/OFF switch number one Foot switch connection (auto pressure control models only) Exterior vacuum pump receptacle (230V shown) Plug vacuum pump in here for shared vacuum pump use.
Chapter 3: Getting Started Installation of Gloves to the Glove Ports With the thumbs up and in a right/left orientation, secure the gloves in place on the glove ports by stretching the beaded glove cuff into the glove port groove nearest the window. Install the separate 8" diameter O-ring over the gloves, into the outer groove of the glove port surface. Stainless steel band clamps are provided for securing the separate O-ring into the glove port groove. Replacement gloves and parts are listed in Chapter 7.
Chapter 3: Getting Started Connect the Plumbing Lines for Gas, Vacuum, and Gas Purifiers/Drying Trains Labconco offers various vacuum and gas tubing installation kits listed in Chapter 7 for connecting inert gas, vacuum, and gas purifiers to the glove box. Refer to Figures 3-4, 3-5, 3-6, 3-7, and 3-8 that depict the installation tubing kits. From Chapter 7, order Gas and Vacuum Tubing Connection Kit 5245100 or 5245200 and install using Figures 3-4 through 3-8.
Chapter 3: Getting Started Accessory AtmosPure ReGen Gas Purifier Circulation Connections (3/4" compression fittings) Rotary vane vacuum pump electrical connection Electronic solenoid valves for glove box models with auto pressure control Inert gas connections (3/8" compression) Vacuum line connections (3/8" compression) Figure 3-4 Required Plumbing Connections – Rear view of glove box with auto pressure control 18 Product Service 1-800-522-7658
Chapter 3: Getting Started (only used with flexible lines for soft tube support during compression) Figure 3-5 Typical Orientation of Ferrules Figure 3-6 Alternate Tube Connection Using Hose Connector Product Service 1-800-522-7658 19
Chapter 3: Getting Started Figure 3-7 Rear View of Glove Box with auto pressure control, all plumbing connections, and accessory AtmosPure Re-Gen Gas Purifier with plumbing connections 20 Product Service 1-800-522-7658
Chapter 3: Getting Started Figure 3-8 Rear View of Glove Box with manual control valves and the plumbing connections Validating the Glove Box Each Protector Glove Box has been helium leak tested at the factory for integrity and found to have no leaks greater than 1 x 10-6 cc/sec while at a positive pressure of 5 inches helium. Refer to Chapter 6, Initial Certification and Appendix G for Helium Leak Test Method.
Chapter 3: Getting Started Protector Stainless CA Glove Box Oxygen Permeation at -4" H2O ISO 10648-2 Test Method 30 25 Barometric Pressure 29.81 inches of Hg Main Chamber Pressure: -4 Inches H2O Time Minutes 20 Transfer Chamber Pressure: 0 inches of Hg 15 Leak Rate Box 5 4.7x10¯ 10 Class 1 < 0.0005 Permeation 0.
Chapter 3: Getting Started Protector Fiberglass CA Glove Box Oxygen Permeation at -4" H2O ISO 10648-2 Test Method 120 100 Barometric Pressure 29.61 inches of Hg Main Chamber Pressure: -4 Inches H 2O Time Minutes 80 Transfer Chamber Pressure: 0 inches of Hg 60 Leak Rate Box 5 8.7x10¯ 40 Class 1 < 0.0005 Permeation 0.
Chapter 3: Getting Started Table 3-1 Classification of Glove Box Containment per ISO 10648-2 Class Hourly Leak Rate (Tf) 1 2 ≤ 5 x 10-4 hr-1 < 2.
Chapter 3: Getting Started Pressure Change Method Leak Testing Summary (ISO-10648-2) The method consists of measuring the pressure rise per unit time after establishing a negative pressure in the main chamber of the glove box. Use -1000 Pa for the acceptance test and -250 Pa for the operational test. The hourly leak rate includes adjustments for barometric pressure and temperature changes which can be calculated as Tf: Tf = (60/t) * ((PnT1/P1Tn) -1) where t is the duration of the test in minutes.
Chapter 4: Performance Features and Safety Precautions Performance Features The Protector Controlled Atmosphere Glove Box comes fully equipped with four manual valves, pressure relief bubbler, and pressure monitoring of the main chamber and transfer chamber. Manual Control models utilize analog gauges and Auto Pressure Controller models utilize automatic control with digital LCD display gauges.
Chapter 4: Performance Features and Safety Precautions • Performance tested to maintain oxygen levels as low as 0.2 ppm and moisture levels as low as 1 ppm. • Oxygen permeation <0.16 ppm/min per ISO 10648-2. Exceeds Class 1 conditions at <1.67 ppm/min. • Illumination exceeds 80 foot candles. • Doublewide models include 4 glove ports with main chamber volume of 34 cu. ft. 3. Basic Manual Control Models include: • Main chamber pressure analog gauge with readings from -5 to +5 inches of water gauge.
Chapter 4: Performance Features and Safety Precautions 11. Interior right-hand side wall-mounted electrical duplex receptacle with 10 amp maximum (100/115 volt models) or 10 amp maximum (230 volt models) circuit load. Doublewide models include an extra interior receptacle. 12. Interior left-hand side wall-mounted pass-through plug for on-site installation of an optional accessory; electrical outlet, RS-232 port, gas/liquid port, or port for oxygen or moisture monitor. 13.
Chapter 4: Performance Features and Safety Precautions Figure 4-1 Performance Features Product Service 1-800-522-7658 29
Chapter 4: Performance Features and Safety Precautions Safety Precautions 1. It is the responsibility of the user to determine the suitability of this product for the intended applications. Consult your Safety Officer for application review. 2. Although the glove box has been engineered to maintain optimum operator safety, caution should always be used while working. Prior to using the glove box, check to make sure that the main chamber pressure is set for comfortable glove manipulation.
Chapter 4: Performance Features and Safety Precautions 12. All powders, gases, and particulates removed by the operation of the vacuum pumps connected to this glove box are to be filtered and/or ducted to the outside. 13. Exhaust vacuum pumps should be filtered and/or ducted to the outside when using hazardous materials. Consult your Safety Officer.
Chapter 5: Using Your Glove Box Now that the installation of your glove box is completed, you are ready to use it. Read this chapter to learn about: • Routine Daily Work Procedures. • Hazardous Use with Chemicals. • Prohibited Acid Use. • Auto Pressure Controller operation. Routine Daily Work Procedures Planning • Thoroughly understand procedures and equipment required before beginning work. Work from a written plan.
Chapter 5: Using Your Glove Box • Perform evacuation and fill cycle on the transfer chamber using the vacuum outlet and gas inlet valves. Depending on the vacuum level achieved, it takes 1-5 cycles to reduce oxygen and moisture levels below 1%. Porous materials being transferred may require longer sustained vacuum purges to remove the oxygen and moisture trapped in the materials.
Chapter 5: Using Your Glove Box • Objects in contact with previously contaminated internal atmospheres must be surface decontaminated or bagged before removal from the glove box through the transfer chamber. • After placing materials to be removed in the transfer chamber and locking the internal door, the transfer chamber must be evacuated and filled approximately 1-5 times depending on vacuum level achieved. This ensures the removal of any remaining airborne contaminants before the outer door is opened.
Chapter 5: Using Your Glove Box Front Panel Description The front panel includes a large display screen and touchpad keys that are adjacent to the LCD display. The operation of these keys will be defined by text on the display screen (LCD). Each key may perform a different operation depending on the control mode selected.
Chapter 5: Using Your Glove Box Main Modes of Operation The Labconco Glove Box Pressure Controller display has four main screens that provide access to the 4 modes of operation listed below. 1.) 2.) 3.) 4.) Glove Box Pressure Control Mode Glove Box Purge and Fill Mode Transfer Chamber Purge and Fill Mode Setup Mode Press the MODE key at the upper-left hand corner of the display screen to switch between each of these four operation modes.
Chapter 5: Using Your Glove Box Glove Box Pressure Control – Operation Mode 1 The Pressure Control mode will allow the user to set a high pressure limit up to +5 inches water column and a low pressure limit to -5 inches water column [+/- 12.6 mBar or +/- 1245 Pacals]. Set these high and low pressure limits with the keys adjacent to the “UP” and “DOWN” arrows on the display.
Chapter 5: Using Your Glove Box Glove Box Evacuation (Purge)/Fill – Operation Mode 2 The Glove Box Purge/Fill mode uses the same solenoid valves on the glove box that are used in the Pressure Control Mode. The Pressure Control mode will be temporarily suspended while a “Purge/Fill” command is being executed.
Chapter 5: Using Your Glove Box Use the “MODE” key in the upper left hand corner adjacent to the icon to return to the “Glove Box Pressure Control” screen to use the “Select” mode. Transfer Chamber Evacuation (Purge) and Fill – Operation Mode 3 The Transfer Chamber Purge/Fill mode controls the pressure changes inside the transfer chamber. Pressure in the transfer chamber will be displayed inside the transfer chamber icon on the display screen.
Chapter 5: Using Your Glove Box Setup Menu – Operation Mode 4 Pressing the key opposite the word “Setup,” allows access to the setup operation mode. In the setup mode, the user may select their preferred units of pressure that are displayed, restore settings back to the default state, or calibrate the pressure sensors. The keys adjacent to the up and down arrows on the lower right of the display are used to select one of the three options. After toggling up or down, push the NEXT key to select the option.
Chapter 5: Using Your Glove Box Option 2 Restore Defaults: The restore defaults option restores all control settings to the initial programmed settings which are listed in the back of these operating instructions. Option 3 Calibration: The pressure sensors inside the controller can be calibrated using atmospheric pressure (single point calibration) or atmospheric pressure and a specific pressure level (two point calibration).
Chapter 5: Using Your Glove Box Back to Previous Screen Yes, All Doors Open To calibrate the pressure sensors for both chambers, the doors must be open so that atmospheric pressure is present at both sensors. This is the “zero point” for both sensors. Both pressure gauges on the glove box will also read “zero.” With both doors open, press the key adjacent to “Yes.
Chapter 5: Using Your Glove Box Main Glove Box Chamber Select Key Transfer Chamber Select Key Back to Previous Screen Save Zero Point This display screen confirms that the doors are open and the sensors are ready for auto-zeroing. First, select the Main Glove Box Chamber key (Box) and wait for complete calibration to zero pressure. Next, select the Transfer Chamber key (CH) and wait for complete calibration to zero pressure.
Chapter 5: Using Your Glove Box Auto Pressure Controller Specifications Glove Box Pressure Control Operating Range Default Settings Pressure Control Limits -5 inches of water column to +5 inches of water column; [+/- 12.6 mBar] Purge (Gas out); Fill (Gas in) Whenever pressure outside limits +/- 5 inches of water column; [+/- 12.6 mBar].
Chapter 6: Maintaining Your Glove Box Review this chapter on maintenance for the following: 1. 2. 3. 4. 5. 6. Routine maintenance. Initial certification. Recertification. Fluorescent light replacement. Solenoid valve replacement for Auto Pressure Controller, if equipped. Auto Pressure Controller circuit board replacement. Routine Maintenance Schedule Weekly • Wipe down the interior surfaces of the glove box with a disinfectant or cleaner, depending upon the application.
Chapter 6: Maintaining Your Glove Box Annually • Replace the fluorescent lamps. • Have the glove box certified by a qualified technician. See Certification and Recertification in Chapter 6. Initial Certification Each Protector Glove Box has been leak tested at the factory with helium gas at positive 5" w.g. pressure; no leaks greater than 1 x 10-6 cc/sec are allowable. The glove box should be certified by your Safety Officer before use, with one of the two tests listed below under Recertification.
Chapter 6: Maintaining Your Glove Box Fluorescent Bulb Spring Clip Fluorecent Bulbs Front panel screw location Fluorescent Bulb Light Socket Figure 6-1 Fluorescent Lights Product Service 1-800-522-7658 47
Chapter 6: Maintaining Your Glove Box Solenoid Valve Replacement 1. Decontaminate the glove box interior, if required. 2. Wear appropriate protective equipment as determined by your Safety Officer. 3. Turn the glove box and vacuum pump OFF. Turn the manual valves on the control panel to closed. 4. Remove the rear access panel and the solenoid valve located on the rear of the Auto Pressure Controller. See Figures 3-4 and 4-1. 5. Replace with the new solenoid valve with proper IN or OUT airflow direction.
Chapter 6: Maintaining Your Glove Box Typical circuit board mounting screw Transfer chamber pressure hose connection attached to port nearest circuit board. Main chamber pressure hose connection attached to port nearest circuit board.
Chapter 7: Accessorizing Your Glove Box There are many ways to accessorize and modify the controlled atmosphere glove box for your individual requirements. These include many different accessories listed in this chapter. 1.
Chapter 7: Accessorizing Your Glove Box 5. Electrical Power Strips These convenience electrical power strips are available in US and international versions and plug into the interior receptacle of the glove box. Part # 5211500 5210601 5290401 5332601 Descriptions US Power Strip 115V, 4 Position, 10A UK Power Strip 250V, 6 Position, 10A European Power Strip 250V, 4 Position, 10A Australian Power Strip 250V, 6 Position, 10A 6.
Chapter 7: Accessorizing Your Glove Box Part # 5121810 5121831 5121835 5121840 5121845 Descriptions 100-115 volts, 50/60 Hz 208-230 volts, 50/60 Hz 208-230 volts, 50/60 Hz 208-230 volts, 50/60 Hz 208-230 volts, 60 Hz Electrical Receptacle & Plug Type North America, 100-115 Volts British (UK) Schuko China/Australia North America, 208-230 Volts 9.
Chapter 7: Accessorizing Your Glove Box 14. Moisture Monitors/Hygrometers Moisture monitors or hygrometers allow the user to continuously monitor moisture levels inside the glove box. Moisture monitors are normally placed inside the main chamber to provide the best performance. Never operate a transfer chamber vacuum evacuation on these sensitive hygrometers as damage can occur.
Chapter 7: Accessorizing Your Glove Box 17. AtmosPure 6-pin Communication Cable (Part #5442000) Required for use when AtmosPure and glove box configured with Auto Pressure Controller are sharing one vacuum pump. 18. AtmosPure 9-pin Oxygen/Moisture Monitor Cable (Part #5442100) Required for use when the AtmosPure is used in AUTO mode. Allows for automatic control of the AtmosPure blower from an accessory Oxygen or Moisture Monitor (sold separately). 19.
Chapter 7: Accessorizing Your Glove Box 21. Drying Train Tubing Kit Includes the (FEP) tubing and connections required for connecting typical drying train components such as moisture, oxygen, and other gases. Fluorinated ethylene-propylene (FEP) tubing has very low moisture absorption. Part # 5242500 5242501 Descriptions Drying Train Tubing Kit; moisture, solvents and radioisotopes Drying Train Tubing Kit; oxygen and moisture only 22. Gas and Vacuum 0.
Chapter 7: Accessorizing Your Glove Box 29. Left Side Door Option (Factory Prepared Only) Any stainless steel glove box can be factory prepared with a large sealed left side door for use in conveniently loading large equipment. With the left side door option, the need to remove the front laminated safety glass frame is eliminated. The left side door has an opening of 16.5" (419 mm) wide x 23.2" (589 mm) high. Contact Labconco at time of order for the left side door option.
Chapter 8: Troubleshooting Refer to the following table if your glove box fails to operate properly. If the suggested corrective actions do not solve your problem, contact Labconco for additional assistance. PROBLEM CAUSE CORRECTIVE ACTION Lights will not operate. Unit not plugged into outlet. Plug unit into appropriate electrical service. Circuit breaker(s) tripped. Reset or replace circuit breaker. Lamp not installed properly. Inspect lamp installation. Lamp wiring disconnected.
Appendix A: Replacement Parts The components that are available for your glove box are listed. The parts shown are the most commonly requested. If other parts are required, please contact Product Service. Item Qty. Part Number 1A 1B 2A 2B 2C 2D 3A 3B 3C 3D 3E 3F 3G 3H 1 1 1 1 0.01 quart 0.
Appendix A: Replacement Parts Item Qty.
Appendix A: Replacement Parts Figure A-1 Replacement Parts 60 Product Service 1-800-522-7658
Appendix B: Dimensions See the following for all dimensions of the glove boxes. For conversion, 1" equals 25.4mm. All dimensions in inches.
Appendix C: Specifications This Appendix contains technical information about all the glove boxes including electrical specifications and environmental operating conditions. • All models: 12 Amps, 100-115V or 230V, 50/60 Hz • Vacuum pump exterior receptacle rated at 12 Amps, 100-115V, 50/60 Hz or 6 Amps, 230V 50/60 Hz. • Single width units require two line cords and double width units require three line cords. Environmental Conditions 62 • Indoor use only.
Appendix D: Controlled Atmosphere Oxygen & Moisture Removal Rates Protector CA (Stainless Steel) Oxygen Removal with AtmosPure Re-Gen Gas Purifier 1000000 210000 100000 42000 10000 Oxygen (PPM) 10000 653.6, 220 cycles stop and AtmosPure circulation blower starts and isolation valves are opened. 3300 1000 100 55.7 26.2 14.1 7.7 10 4.2 2.4 1.5 1 0 50 100 150 1.1 0.8 0.7 200 250 0.1 Time (Minutes) This chart shows oxygen level decrease from 210,000 ppm at atmospheric conditions to 0.
Appendix D: Controlled Atmosphere Oxygen & Moisture Removal Rates Protector CA (Stainless Steel) Moisture Removal with AtmosPure Re-Gen Gas Purifier 100000 11253, 40% RH 10000 Moisture (PPM) 1560 696 1000 167.5, 220 cycles stop and AtmosPure circulation blower starts and isolation valves are opened. 445 100 87.5 15.9 10 8.5 3.5 2.4 1.8 1.0 1 1 10 100 1000 Time (Minutes) This chart shows moisture level decrease from 11,253 ppm (40% relative humidity) to 1.0 ppm after 8965 minutes.
Appendix D: Controlled Atmosphere Oxygen & Moisture Removal Rates Protector CA (Stainless Steel) Oxygen Removal with AtmosPure Re-Gen Gas Purifier 1000 753.6 100 55.7 Oxygen (PPM) 26.2 14.1 10 7.7 4.2 2.4 1.1 1.5 1 0 20 40 60 80 100 120 140 0.8 0.1 Time (Minutes) This chart shows oxygen level decrease from 753.6 ppm to 0.7 ppm after 150 minutes. Two separate calibrated oxygen monitors confirmed results. Product Service 1-800-522-7658 160 0.
Appendix D: Controlled Atmosphere Oxygen & Moisture Removal Rates Protector CA (Stainless Steel) Moisture Removal with AtmosPure Re-Gen Gas Purifier 1000 853.2 87.5 Moisture (PPM) 100 15.9 8.5 10 3.5 2.4 1.8 1.0 1 1 10 100 1000 Time (Minutes) This chart shows moisture level decrease from 853.2 ppm to 1.0 ppm after 8905 minutes. Two separate calibrated moisture monitors confirmed results.
Appendix D: Controlled Atmosphere Oxygen & Moisture Removal Rates Protector CA (Fiberglass) Oxygen Removal with AtmosPure Re-Gen Gas Purifier 1000 969.2 100 63.9 Oxygen (PPM) 28.0 15.3 10 7.9 4.3 2.4 1.2 0.9 1.6 1 0 20 40 60 80 100 1.0 120 0.77 140 0.1 Time (Minutes) This chart shows oxygen level decrease from 969.2 ppm to 0.77 ppm after 150 minutes. Two separate calibrated oxygen monitors confirmed results.
Appendix D: Controlled Atmosphere Oxygen & Moisture Removal Rates Protector CA (Fiberglass) Moisture Removal with AtmosPure Re-Gen Gas Purifier 1000 600 100 Moisture (PPM) 50.1 10.6 10 6.7 4.6 3.5 2.3 1 1 10 100 1000 Time (Minutes) This chart shows moisture level decrease from 600 ppm to 2.3 ppm after 9875 minutes. Two separate calibrated moisture monitors confirmed results.
Appendix E: Approximate Life Span for Accessory AtmosPure Re-Gen Gas Purifier Protector (FRP) Fiberglass Protector Stainless Steel 0.4 ppm 0.2 ppm 0.16 ppm/min 0.
Appendix F: Ionizer Fan Performance Criteria Static Electricity Test The tests were performed by monitoring the level of static electricity found on the work surface and interior surfaces within the glove box. Static electricity levels were measured with an AlphaLab Surface DC Voltmeter http://scientificmeter.com/surface_dc.htm. A DESCO Emit Mini Zero Volt Ionizer http://www.descoemit.com/ViewProduct.aspx?pid= 50661&h=1323 was installed in the glove box.
Appendix F: Ionizer Fan Performance Criteria Electrostatic with Glove Box Blower & Ionizer Fan OFF 100 CFM Blower & Ionizer Fan ON After 1 Hour 100 CFM Blower & Ionizer Fan ON After 2 Hours Metal Housing Inlet HEPA + 420 Test Point Locations and Voltage Levels Metal Metal Back Left Right Housing Transfer Interior FRP FRP Exhaust Chamber Surface Interior Interior HEPA Handle Surface Surface + 220 + 734 + 375 + 106 + 151 Center FRP Work Surface + 153 + 96 + 114 + 290 + 93 + 45 + 52 + 35 + 28 + 58
Appendix G: Helium Leak Test Procedure Purpose The purpose of this test is to assure that all gasketed joints and seams of the enclosure are helium leak tight. Test Setup 1. Connect one of the valves to a vacuum pump. 2. Connect the other valve to a helium supply tank regulated between 10 and 15 PSI. 3. Install two neoprene gloves onto the glove ports using standard Orings and clamps. Test Procedure 1. Be sure the inner transfer chamber door is latched closed and the outer door is open. 2.
Appendix G: Helium Leak Test Procedure Other Equipment 1. For a less critical helium leak measurement using a lower cost leak detector, Labconco recommends using Restek (www.restek.com) or other instrumentation available from a laboratory supply dealer.
Appendix H: Conversions Pressure Conversions To From mmHg in.Hg in.H2O ft.H2O atm lb/in.2 Kg/cm2 kPa bar mmHg in.Hg in.H2O ft.H2O atm lb/in.2 Kg/cm2 kPa bar 1 25.40 1.868 22.42 760 51.71 735.6 7.500 750 .03937 1 .07355 .8826 29.92 2.036 28.96 .2953 29.53 .5353 13.60 1 12 406.8 27.69 393.7 4.016 401.6 .04461 1.133 .08333 1 33.90 2.307 32.81 .3347 33.47 .00132 .03342 .00246 .02950 1 .06805 .9678 .00987 .987 .01934 .4912 .03612 .4334 14.70 1 14.22 .1451 14.51 .00136 .03453 .00254 .03048 1.033 .
Appendix I: References Many excellent reference texts and booklets are currently available. The following is a brief listing: Pharmaceutical Isolators, A Guide to their application design and control. Pharmaceutical Press 2004, Editors: Midcalf, Phillips, Neiger, and Coles. Isolation Technology, A Practical Guide, 2004 CRC Press, 2nd Edition, www.crcpress.com International Standard ISO 10648-2 Containment Enclosures • Classification according to leak tightness and associated checking methods.
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