User’s Manual Precise® HEPA-Filtered Glove Boxes Models 5220200 5220220 5220221 5220230 5220231 Voltage, Frequency 115V, 60 Hz 230V, 50 Hz 230V, 60 Hz 100V, 50 Hz 100V, 60 Hz To receive important product updates, complete your product registration card online at register.labconco.com Labconco Corporation 8811 Prospect Avenue Kansas City, MO 64132-2696 800-821-5525, 816-333-8811 FAX 816-363-0130 E-MAIL labconco@labconco.com HOME PAGE www.labconco.
Copyright © 2007, 2008 Labconco Corporation. All rights reserved. The information contained in this manual and the accompanying products are copyrighted and all rights reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change. Warranty Labconco provides a warranty on all parts and factory workmanship.
TABLE OF CONTENTS CHAPTER 1: INTRODUCTION 1 CHAPTER 2: PREREQUISITES Support, Vibration & Preventive Requirements Location Requirements Exhaust and Blower Requirements ISO Class 3 Definition Electrical Requirements Space Requirements 2 3 3 3 5 7 7 CHAPTER 3: GETTING STARTED Unpacking the Glove Box Installing the Glove Box on a Supporting Structure & Work Surface Connecting to the Exhaust System (If Applicable) Installation of Gloves to the Glove Ports Connecting the Electrical Supply to the Glove Box Se
CHAPTER 6: MAINTAINING YOUR GLOVE BOX Routine Maintenance Schedule Decontamination Determining When to Replace HEPA Filters How to Install New HEPA Filters HEPA Filter Leak Test Initial Certification Re-Certification Fluorescent Light Replacement Motorized Impeller Replacement Speed Control Replacement 27 28 29 29 29 31 33 33 33 34 34 CHAPTER 7: ACCESSORIZING YOUR GLOVE BOX 35 CHAPTER 8: TROUBLESHOOTING 41 APPENDIX A: REPLACEMENT PARTS 42 APPENDIX B: DIMENSIONS 44 APPENDIX C: SPECIFICATIONS 45 A
Chapter 1: Introduction Congratulations on your purchase of a Labconco Precise® HEPA-Filtered Glove Box. Your glove box provides personnel protection through superior containment rated at Class 1 conditions for hazardous environments, per ISO 10648-2. These glove boxes will effectively contain toxic or noxious particulates when properly installed and operated. In positive or negative pressure operation modes, the glove box can create an ISO standard Class 3 (ISO 14644-1) clean air environment.
Chapter 2: Prerequisites Before you install the glove box, you need to prepare your site for installation. You must be certain that the area is level and of solid construction. In addition, a dedicated source of electrical power should be located near the installation site to power the glove box. The glove box should be strategically placed in the lab to provide efficient workflow and prevent operator interference from normal traffic patterns.
Chapter 2: Prerequisites Support, Vibration and Preventive Requirements In the preparation of a glove box site, please consider the following: • A bench or stand that is rigidly mounted to the floor or fixed to the wall, but not both, may be appropriate. 35" to 40" (889mm-1016mm) is typical for standing height. (Labconco stands offered in Chapter 7 vary from 33" to 40".) • The corners of a building typically have less vibration than the center, which promotes analytical balance stability.
Chapter 2: Prerequisites Labconco does not recommend hard duct connections with remote blowers due to the air volume balance requirements with such systems. Hard duct connections should only be used when the glove box impeller is disconnected and only a single roof-mounted blower is used. Note that reliance on an external exhaust blower does not provide airflow control at the glove box.
Chapter 2: Prerequisites Precise HEPA-Filtered Glove Box Particle Testing under Positive Pressure 500 NOTE: Ambient Air Baseline Particle Count at 1 Minute 198671 Particles measuring 0.3 Micron detected within 1 Minute. 25473 Particles measuring 0.5 Micron detected within 1 Minute. Number of Particles (Per Cubic Meter Per Minute) 450 400 350 306 300 Airflow Testing Performed at 50 Dial Setting (61 CFM) with Glove Box Under Positive Pressure. 250 200 139 150 > 0.3 Micron 100 > 0.
Chapter 2: Prerequisites Additionally, the motorized impeller can also be used in a full recirculation mode providing the operator with some control of the atmosphere chemistry within the glove box. See Chapter 7 for the Recirculation Conversion Kit. The airflow chart below represents typical operational data including air changes per minute, exhaust volume, noise levels, main chamber static pressures, and total system static pressures measured at the blower intake (suction side).
Chapter 2: Prerequisites For thimble-ducted glove boxes, the proper remote blower selection can be determined from these exhaust requirements as the remote blower only needs to be sized for the static pressure loss of the ductwork system. The thimble ducted exhaust blower should be sized for 25% additional airflow. When exhausting a glove box to the outside, it should be connected to a dedicated remote blower. Labconco offers accessory remote blowers listed in Chapter 7.
Chapter 3: Getting Started Once the site for your Precise Glove Box is properly prepared, you are ready to unpack, inspect, install, and validate your system. Read this chapter to learn how to: • Unpack and move the glove box. • Set up the glove box with the proper supporting structure and work surface. • Connect to an exhaust system if applicable. • Install the gloves. • Connect the electrical supply. • Set the exhaust volume with the speed control adjustment.
Chapter 3: Getting Started Figure 3-1 Lifting Instructions Unpacking the Glove Box We recommend that you do not remove the glove box from its shipping container until it is ready to be placed into its final location. Move the unit by placing a flat, low dolly under the shipping skid, or by using a floor jack. Carefully remove the carton shrink-wrap on the glove box and inspect it for damage that may have occurred in transit.
Chapter 3: Getting Started ) IF ENCLOSURE WAS DAMAGED IN TRANSIT, YOU MUST FILE A CLAIM DIRECTLY WITH THE FREIGHT CARRIER. LABCONCO CORPORATION AND ITS DEALERS ARE NOT RESPONSIBLE FOR SHIPPING DAMAGES. Do not discard the packing material until you have checked all of the components and tested the glove box. Installing the Glove Box on a Supporting Structure and Work Surface Exercise caution when lifting or moving the glove box.
Chapter 3: Getting Started Figure 3-2 Exhaust Connection to Outside (All dimensions in inches) Product Service 1-800-522-7658 11
Chapter 3: Getting Started Figure 3-3 Positive Pressure Glove Box Configuration Installation of Gloves to the Glove Ports With thumbs up and right/left orientation, secure the gloves in place on the glove ports by stretching the beaded cuff into the groove nearest the window. Install the separate 8" diameter O-ring over the gloves and into the outer groove of the glove port surface. Stainless steel band clamps are provided for securing the separate O-ring into the glove port groove.
Chapter 3: Getting Started Connecting the Electrical Supply to the Glove Box 115V, 60 Hz; 100V, 50 Hz; or 100V, 60 Hz Models Connect the power cord supplied to the IEC electrical supply plug on the back of the glove box. 115V or 100V models are rated at 8 amps total. The maximum circuit load for the interior electrical duplex is 5 amps. 230V, 50 Hz or 230V, 60 Hz Models The same procedure applies for the 230V model except it is shipped without a plug. Install the appropriate plug per local requirements.
Chapter 3: Getting Started Validating the Glove Box The Precise HEPA-Filtered Glove Box has been leak tested at the factory for HEPA filter and main chamber pressure integrity. The HEPA filters should be leak checked upon start-up and annually thereafter per the procedure in Chapter 6. Upon start up, the main chamber pressure should be greater than 0.7" w.g. at full blower speed to validate proper blower operation; if not, contact Labconco for service.
Chapter 3: Getting Started • • • • • and 33.7 foot candles with the test lab’s overhead fluorescent lights turned on. Static pressure measured inside the glove box varied from negative 0.14 inch of water column at minimum 13-CFM to negative 0.91 inch of water at maximum 100-CFM. The total system static pressure measured between the exhaust filter and the blower varied from negative 0.34 inch of water column at 13-CFM to negative 2.4 inches of water at 100-CFM.
Chapter 4: Performance Features and Safety Precautions Performance Features The Precise HEPA-Filtered Glove Box is designed to meet the needs of the laboratory scientist, and provide superior particulate containment. The HEPA-filtered glove boxes have been tested to effectively contain toxic and noxious materials when properly installed and operated. Labconco engineered the HEPA-Filtered glove boxes to minimize the effects of turbulence.
Chapter 4: Performance Features and Safety Precautions 4. Main Chamber Static Pressure Gauge provides an indication of HEPA filter condition inside the glove box. Typically, HEPA filters should be replaced when the static pressure gauge reading has increased two-fold. The gauge can be changed to positive pressure for clean air glove box operations by reversing the hose on the gauge to the positive pressure connection. Positive Pressure Conversion Kit is sold separately (see Chapter 7). 5.
Chapter 4: Performance Features and Safety Precautions Figure 4-1 18 Product Service 1-800-522-7658
Chapter 4: Performance Features and Safety Precautions Safety Precautions 1. It is the responsibility of the user to determine the suitability of this product for the intended applications. Consult your Safety Officer. 2. Prior to using the glove box, check to make sure that the exhaust blower is operating and that air is entering the glove box by observing the static pressure gauge. 3. Use good housekeeping in the enclosure at all times. Clean up spills immediately. Periodically clean glove box interior.
Chapter 4: Performance Features and Safety Precautions 13. The surface of the HEPA filter is fragile and should not be touched. Care must be taken to avoid puncturing the HEPA filter during installation or normal operation. If you suspect that a HEPA filter has been damaged DO NOT use the glove box; contact a local certification agency or Safety Officer. 14. The HEPA filters in the glove box will gradually accumulate airborne particulate matter from the room and from work performed in the glove box.
Chapter 5: Using Your Glove Box Now that the installation of your glove box is completed, you are ready to use your Precise HEPA-Filtered Glove Box.
Chapter 5: Using Your Glove Box Loading Materials and Equipment (see Figure 5-1) • Load only the materials required for the procedure. Do not overload the glove box. • Be aware that gloves will inflate when the glove box is operated under negative pressure. Ensure items in the glove box will not obstruct the movement of the gloves. • After loading, wait 2-5 minutes at 50-100% blower speed to purge airborne contaminants from the work area before beginning procedures.
Chapter 5: Using Your Glove Box Safety Note: Must seal and wipe down all chemical containers. Figure 5-1 Loading and Final Purging Technique HEPA Filter Applications, Suitability and Guidelines • Microbiological materials and hazardous solids containment protecting only the operator and the surrounding environment. • Weighing of powders. • Containment of hazardous particulates using a HEPA filter. • Powder or particulate procedures traditionally performed on an open bench.
Chapter 5: Using Your Glove Box Definition of Terms NIOSH – National Institute for Occupational Safety and Health/Mine Safety and Health Administration. (U.S.A.) TWA – Recommended Exposure Limits expressed as a Time Weighted Average. The exposure limit for that chemical for up to a 10-hour workday, 40 hours a week. Expressed in units of parts per million or milligrams per cubic meter.
Chapter 5: Using Your Glove Box Chemicals must be designated by NIOSH guidelines as acceptable for use with chemical cartridge-type respirators (the exception is formaldehyde and ammonia/amines, which use impregnated carbon). Chemicals not listed by NIOSH in the Pocket Guide must be approved by Labconco Product Specialist (or Engineering). Inlet concentration must never exceed the IDLH (Immediately Dangerous to Life and Health) concentrations.
Chapter 5: Using Your Glove Box The inlet concentration or the evaporation rate of the chemical must never exceed the TWA for the chemical. Consult a Labconco Technical Specialist for estimated saturation life. See Chapter 6 for an example of estimating saturation life based on Labconco’s chemical guide. Prohibited Acid Use The Precise HEPA-Filtered Glove Box and internal metal parts must not be exposed to mineral acid use.
Chapter 6: Maintaining Your Glove Box Review this chapter on maintenance for the following: 1. Routine maintenance. 2. Decontamination. 3. Determining when to replace the HEPA filters. 4. How to install new HEPA filters. 5. HEPA filter leak test. 6. Initial certification. 7. Re-certification. 8. Fluorescent light replacement. 9. Motorized impeller replacement. 10. Speed control replacement.
Chapter 6: Maintaining Your Glove Box Routine Maintenance Schedule Weekly • Wipe down the interior surfaces of the glove box with a disinfectant or cleaner, depending upon the application. • Using a damp cloth, clean the exterior surfaces of the glove box, particularly the front and top, to remove any accumulated dust. • Operate the exhaust system, noting the airflow through the glove box to effectively remove a source of visible smoke.
Chapter 6: Maintaining Your Glove Box Decontamination When used in conjunction with microbiologicals, the glove box should be decontaminated with formaldehyde gas before: • Performing maintenance work in contaminated areas • HEPA filter changes • Moving the glove box to a new location • Changing research programs The procedures for performing a gaseous decontamination are thoroughly outlined in the U.S.
Chapter 6: Maintaining Your Glove Box 4. Place both arms in the rear gloves of the bag, and carefully draw the HEPA filter out of the glove box. Rest the HEPA filter on the floor, table or cart. Remove arms from bag gloves. 5. Create two seals between the glove box housing and the old HEPA filter. Seals can be heat sealed or sealed with tie wraps. Cut off the bag between the two seals and leave the bag stub on the access port to the glove box. 6. Place the new HEPA filter in another new bag.
Chapter 6: Maintaining Your Glove Box HEPA Filter Leak Test Purpose This test is performed to determine the integrity of the HEPA filter(s), the filter housing, and the filter mounting frames. The leak test is to be done by a qualified technician with calibrated equipment. Reference Leak Testing and Photometer scanning from the Institute of Environmental Services (IES-RPCC001.3) Equipment Required 1. An aerosol photometer ATI model 2D, 2E, 2G or equivalent. Air Techniques Hamilton Associates inc.
Chapter 6: Maintaining Your Glove Box 7. Ensure that the glove box speed control is adjusted to the maximum setting and the blower is exhausting 100 CFM through the glove box. 8. Position the aerosol generator discharge near the internal opening of each HEPA filter. Check both filters. 9. Start the aerosol generator. Ensure that the generator is operating with 1 nozzle on at 10 PSIG. 10. Allow the generator to operate for a minimum of 15 seconds.
Chapter 6: Maintaining Your Glove Box the blower inlet (suction side) test port. Connect this tube to the “downstream” sampling port of the photometer. Acceptance The downstream average penetration shall not exceed 0.005 percent as measured by the photometer. Initial Certification The Precise HEPA-Filtered Glove Box has been leak tested at the factory with helium gas at positive 1" w.g. pressure; leaks cannot exceed 1 x 10-3 cc/sec.
Chapter 6: Maintaining Your Glove Box Motorized Impeller Replacement The motorized impeller must be replaced as a complete unit. See Appendix A for replacement parts diagram. 1. Unplug the motorized impeller from the electrical outlet. Refer to Appendix A for replacement parts. 2. Remove the hose connecting the exhaust HEPA filter to the motorized impeller. 3. Remove the motorized impeller and support braces from the light and the rear sheet metal façade. 4.
Chapter 7: Accessorizing Your Glove Box There are many ways to accessorize and modify the glove box for your individual requirements. These include many different accessories listed in this chapter. 1. Black Epoxy Flat Work Surface (Part #4882807) A solid epoxy resin 60" x 30" x 1.25" work surface is available to support the glove box. 2. 30" Standard Base Cabinet (Part #9900200) Two optional base cabinets can be ordered to support the 60" x 30" work surface and glove box.
Chapter 7: Accessorizing Your Glove Box 8. Pressure Gauge for the Transfer Chamber (Part #5238100) Kit includes the pressure gauge, hose and fittings to add to the transfer chamber. Range 0 to 29 inches of mercury vacuum. 9. Add-A-Valve Kit (Part #5225701) Includes the valve, O-ring, and nut to supply the main chamber or the transfer chamber to purge vacuum, fill gas, or connect a drying train. One each Identifier Label Set for valves (Part #5236600) is ordered separately. 10.
Chapter 7: Accessorizing Your Glove Box Part # 3970000 3970020 3970001 3970021 3970002 3970022 3970003 3970023 3970004 3970024 3707900 3923400 3923401 3923402 3776002 Descriptions 115V FilterMate (HEPA only included) 230V FilterMate (HEPA only included) 115V FilterMate (Carbon Filter sold separately) 230V FilterMate (Carbon Filter sold separately) 115V FilterMate w/thimble (HEPA included) 230V FilterMate w/thimble (HEPA included) 115V FilterMate Combination HEPA/Carbon (order Carbon Filter separately) 230V
Chapter 7: Accessorizing Your Glove Box 20. Remote Blowers Has a 1/4 hp direct drive motor and corrosion-resistant epoxy-coated steel housing and wheel with blower inlet of 6.00" ID. Outlet dimensions are 4.25" x 7.38" OD. S.P. CFM Part # 4863500 4863501 0.0" 595 CFM @ Static Pressure-Inches of H2O 0.125" 0.25" 0.50" 0.75" 560 515 420 300 0.87" 167 Description Remote Blower, 115 V, 60 Hz. 4.4 amps Remote Blower, 115/230 V, 50 Hz, 5.6/2.8 amps Figure 7-1 All dimensions in inches 21.
Chapter 7: Accessorizing Your Glove Box Part # 7995600 5244100 5240100 Descriptions Ammonia Trap Insert Flowmeter, Visual Gas & Vacuum 0.2 Micron Filter 24. Acrylic Viewing Window with Frame (Part #5236100) Acrylic window, 1/2" thick, for those users that prefer acrylic over the standard laminated safety glass (includes metal frame). 25. Guardian Jr.
Chapter 7: Accessorizing Your Glove Box 31. Drying Train Tubing Kit Includes the (FEP) tubing and plumbing fittings for connecting typical moisture, and other gas fume removal components (see item 23 above). Fluorinated ethylene-propylene (FEP) tubing has very low moisture absorption. Part # 5242500 Descriptions Drying Train Tubing Kit; Moisture, Solvents, and Radioisotopes 32. HEPA and ULPA Filters HEPA filters come standard with the glove box. ULPA filters may be substituted as an upgrade.
Chapter 8: Troubleshooting Refer to the following table if the Precise HEPA-Filtered Glove Box fails to operate properly. If the suggested corrective actions do not solve your problem, contact Labconco for additional assistance. PROBLEM CAUSE CORRECTIVE ACTION Blower won’t operate. Unit not plugged into outlet. Circuit breaker(s) or Ground Fault Interrupter. Blower wiring is disconnected. Blower switch is defective. Motorized impeller or blower is defective. Unit not plugged into outlet.
Appendix A: Replacement Parts The components that are available for the Precise HEPA-Filtered Glove Box are listed. The parts shown are the most commonly requested. If other parts are required, please contact Product Service. Item Qty.
Appendix A: Replacement Parts Figure A-1 Replacement Parts for Precise HEPA-Filtered Glove Box Product Service 1-800-522-7658 43
Appendix B: Dimensions See the following dimensions and exhaust options for the Precise HEPAFiltered Glove Boxes. All dimensions are in inches.
Appendix C: Specifications This Appendix contains technical information about all the glove boxes including electrical specifications and environmental operating conditions. • 8 Amps, 115V, 50/60 Hz or 4 Amps, 230V, 50/60 Hz or 8 Amps, 100V, 50/60 Hz, Precise HEPA-Filtered Glove Box. Environmental Conditions • Indoor use only. • Maximum altitude: 6562 feet (2000 meters). • Ambient temperature range: 41° to 104°F (5° to 40°C).
Appendix D: Ionizer Fan Performance Criteria Static Electricity Test The tests were performed by monitoring the level of static electricity found on the work surface and interior surfaces within the glove box. Static electricity levels were measured with an AlphaLab Surface DC Voltmeter http://scientificmeter.com/surface_dc.htm. A DESCO Emit Mini Zero Volt Ionizer http://www.descoemit.com/ViewProduct.aspx?pid= 50661&h=1323 was installed in the glove box.
Appendix D: Ionizer Fan Performance Criteria Electrostatic with Glove Box Blower & Ionizer Fan OFF 100 CFM Blower & Ionizer Fan ON After 1 Hour 100 CFM Blower & Ionizer Fan ON After 2 Hours Test Point Locations and Voltage Levels Metal Back Left Right Transfer Interior Interior Interior Chamber Surface Surface Surface Handle + 734 + 375 + 106 + 151 Metal Housing Inlet HEPA + 420 Metal Housing Exhaust HEPA + 220 + 96 + 114 + 290 + 93 + 45 + 28 + 58 + 51 - 52 - 15 Interior Lamp Window Center W
Appendix E: Conversions Pressure Conversions To From mmHg in.Hg in.H2O ft.H2O atm lb/in.2 Kg/cm2 kPa bar mmHg in.Hg in.H2O ft.H2O atm lb/in.2 Kg/cm2 kPa bar 1 25.40 1.868 22.42 760 51.71 735.6 7.500 750 .03937 1 .07355 .8826 29.92 2.036 28.96 .2953 29.53 .5353 13.60 1 12 406.8 27.69 393.7 4.016 401.6 .04461 1.133 .08333 1 33.90 2.307 32.81 .3347 33.47 .00132 .03342 .00246 .02950 1 .06805 .9678 .00987 .987 .01934 .4912 .03612 .4334 14.70 1 14.22 .1451 14.51 .00136 .03453 .00254 .03048 1.033 .
Appendix F: References Many excellent reference texts and booklets are currently available. The following is a brief listing: Pharmaceutical Isolators, A Guide to their application design and control. Pharmaceutical Press 2004, Editors: Midcalf, Phillips, Neiger, and Coles. Isolation Technology, A Practical Guide, 2004 CRC Press, 2nd Edition, www.crcpress.com International Standard ISO 10648-2 Containment Enclosures • Classification according to leak tightness and associated checking methods.
DECLARATION OF CONFORMITY Application Council Directive(s): 73/23/EEC, 89/336/EEC, 2002/95/EC (ROHS), 2002/96/EC (WEEE) Standard(s) to which conformity is declared: EN61010-1, EN61326-1 Manufacturer’s Name: Labconco Corporation Manufacturer’s Address: 8811 Prospect Avenue Kansas City, MO 64132 USA Importer’s Name: See Shipping/Customs Documents Importer’s Address: See Shipping/Customs Documents for your equipment Type of Equipment: Laboratory Equipment Precise Glove Boxes 5220000 Series 5220100 Se
