User’s Manual FreeZone® Benchtop Shell Freezers Models 79490 Series To receive important product updates, complete your product registration card online at register.labconco.com Labconco Corporation 8811 Prospect Avenue Kansas City, MO 64132-2696 800-821-5525, 816-333-8811 FAX 816-363-0130 E-MAIL labconco@labconco.com HOME PAGE www.labconco.com Please read the User’s Manual before operating the equipment.
Copyright © 2004, 2007, 2012 Labconco Corporation. All rights reserved. The information contained in this manual and the accompanying products are copyrighted and all rights reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change. Warranty Labconco provides a warranty on all parts and factory workmanship.
TABLE OF CONTENTS CHAPTER 1: INTRODUCTION Freeze Dry Process Freeze Dry Rates Freeze Dry Capacity Samples Containing Volatile Substances Sample Surface Area and Thickness of the Sample About This Manual Typographical Conventions 1 1 2 3 4 4 5 7 CHAPTER 2: PREREQUISITES Electrical Requirements Location Requirements 8 8 9 CHAPTER 3: GETTING STARTED Unpacking Your Benchtop Shell Freezer Benchtop Shell Freezer Components Installing Your Benchtop Shell Freezer Electrical Connection Solvent Safety Precautions
CHAPTER 1 INTRODUCTION Congratulations on your purchase of a Labconco FreeZone® Benchtop Shell Freezer, which is designed to prepare samples for laboratory lyophilization procedures. The unit is easy to install and maintain. Proper care and maintenance of this product will result in many years of dependable service. Freeze Dry Process Freeze drying is an important process in sample preparation and for the preservation and storage of biologicals, pharmaceuticals and foods.
Chapter 1: Introduction Freeze Dry Rates The efficiency of the freeze drying process is dependent upon the surface area and the thickness of the sample, the collector temperature and vacuum obtained, the eutectic point and solute concentration of the sample. It is important to remember these factors when trying to obtain efficient utilization of your freeze dry system. A listing of selected materials and their approximate drying times are shown in Table 1 for your reference.
Chapter 1: Introduction In order for lyophilization to occur, ice must be removed from the frozen sample via sublimation. This is accomplished by the collector and the vacuum pump. The collector, which should be at least 15 to 20°C colder than the eutectic temperature (melting temperature) of the sample, traps vapor as ice. Since the vapor pressure at the collector is lower than that of the sample, the flow of water vapor is from the sample to the collector.
Chapter 1: Introduction vacuum in the system may recover before adding another sample to the system. If the vacuum does not recover, the capacity of the freeze dry system has been exceeded and the sample should be removed. If there is a problem with a particular type of sample melting when placed on the freeze dry system, dilution of the sample with more water or providing some insulation around the flask to decrease the rate of heat absorption by the sample may help.
Chapter 1: Introduction through the dried portions of the product, which impedes their progress. As the drying front moves further and further down the matrix, the application of heat to the product becomes more important. Shell freezing as a method of prefreezing the product can increase the surface area-to-volume ratio by spreading out the frozen product inside the vessel.
Chapter 1: Introduction Chapter 3: Getting Started contains the information you need to properly unpack, inspect and install your Benchtop Shell Freezer. Chapter 4: Using Your Benchtop Shell Freezer discusses the basic operation and information on how to load samples. Chapter 5: Maintaining Your Benchtop Shell Freezer explains how to perform routine maintenance.
Chapter 1: Introduction Typographical Conventions Recognizing the following typographical conventions will help you understand and use this manual: • • • ! ) • • Book, chapter, and section titles are shown in italic type (e.g., Chapter 3: Getting Started). Steps required to perform a task are presented in a numbered format. Comments located in the margins provide suggestions, reminders, and references.
CHAPTER 2 PREREQUISITES Before you install your Benchtop Shell Freezer, you need to prepare your site for installation. The Benchtop Shell Freezer may be mounted on top of a benchtop work surface. Carefully examine the location where you intend to install your Benchtop Shell Freezer. You must be certain that the area is level and of solid construction. An electrical source must be located near the installation site. Carefully read this chapter to learn: • • the electrical supply requirements.
Chapter 2: Prerequisites Location Requirements The Benchtop Shell Freezer should be located in an area that provides an unobstructed flow of air around the cabinet. This air cools the refrigeration system. A minimum of 3" must be allowed between the back and both sides of the Benchtop Shell Freezer and adjacent wall surfaces. Restriction of airflow during operation could adversely affect performance.
CHAPTER 3 GETTING STARTED Now that the site for your Benchtop Shell Freezer is properly prepared, you are ready to unpack, inspect, install and test your Benchtop Shell Freezer. Read this chapter to learn how to: • • • • ! unpack and move your Benchtop Shell Freezer. set up your Benchtop Shell Freezer. connect the electrical supply source to your Benchtop Shell Freezer. safely use solvents with your Benchtop Shell Freezer. The Benchtop Shell Freezer weighs over 90 lbs. (41 Kg).
Chapter 3: Getting Started ) DO NOT RETURN GOODS WITHOUT THE PRIOR AUTHORIZATION OF LABCONCO. UNAUTHORIZED RETURNS WILL NOT BE ACCEPTED. ) IF YOUR BENCHTOP SHELL FREEZER WAS DAMAGED IN TRANSIT, YOU MUST FILE A CLAIM DIRECTLY WITH THE FREIGHT CARRIER. LABCONCO CORPORATION AND ITS DEALERS ARE NOT RESPONSIBLE FOR SHIPPING DAMAGE. ) DO NOT DISCARD THE CARTON OR PACKING MATERIALS UNTIL YOU HAVE CHECKED ALL OF THE COMPONENTS AND INSTALLED AND TESTED THE BENCHTOP SHELL FREEZER.
Chapter 3: Getting Started Installing Your Benchtop Shell Freezer After you verify receipt of the proper components, move your Benchtop Shell Freezer to the location where you want to install it. Then, follow the steps listed below. 1. Check that the solvent bath drain plug is securely installed in the drain hose. 2. Remove bath compartment cover and add solvent to bath compartment. Commonly used solvents are methanol or ethanol.
Chapter 3: Getting Started WARNING: The disposal of substances used in connection with this equipment may be governed by various Federal, State or local regulations. All users of this equipment are urged to become familiar with any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water, land or air and to comply with such regulations.
CHAPTER 4 USING YOUR BENCHTOP SHELL FREEZER After your Benchtop Shell Freezer has been installed as detailed in Chapter 3: Getting Started, you are ready to begin using your Benchtop Shell Freezer. Read this chapter to learn how to: • • • operate the controls. understand the display. load samples. ! 14 Do not use the Benchtop Shell Freezer in a manner not specified by the manufacturer (refer to Appendix C: Benchtop Shell Freezer Specifications).
Chapter 4: Using Your Benchtop Shell Freezer Benchtop Shell Freezer Controls The control panel for the Benchtop Shell Freezer is shown below with a description about its function. 1. Main Power Switch- (Not shown - Located on right side of cabinet) turns the unit on or off. 2. Bath Temperature Graph Display – This display indicates the temperature of the solvent in the Benchtop Shell Freezer bath. The top LED turns on when the main power switch is turned on.
Chapter 4: Using Your Benchtop Shell Freezer Follow the steps below each time you use the shell freezer to obtain optimum performance: 1. Check that the solvent bath drain plug is securely installed in the drain hose. 2. Remove the bath compartment cover and add solvent to the bath compartment. Commonly used solvents are methanol or ethanol. Recommended solvent depth is 1/4" to 1/2" above the top of the rollers (approximately 2 liters).
CHAPTER 5 MAINTAINING YOUR BENCHTOP SHELL FREEZER Under normal operation, the Benchtop Shell Freezer requires little maintenance. The following maintenance schedule is recommended: As needed: 1. If the heat transfer liquid in the bath becomes contaminated, drain and replace. It is the user’s responsibility to dispose of it in accordance with all applicable regulations. 2. Clean up all spills. 3. Clean the lid and gasket using a soft cloth, sponge or chamois and a mild, non-abrasive soap or detergent.
Chapter 5: Maintaining Your Benchtop Shell Freezer surfaces. Do not use solvents to remove stains from the exterior surfaces as they may damage the finish. Annually: 1. Every 12 months, or more often if the Benchtop Shell Freezer is operated in a dusty environment, the refrigeration system condenser should be cleaned. Using a vacuum cleaner and brush attachment, clean the condenser to ensure proper airflow for peak performance. It can be accessed by removing the front lower panel.
APPENDIX A BENCHTOP SHELL FREEZER COMPONENTS The following pages list components that are available for your Benchtop Shell Freezer. The parts shown are the most common replacement parts. If other parts are required, contact Product Service.
Appendix A: Benchtop Shell Freezer Components Item Qty Part No.
APPENDIX B BENCHTOP SHELL FREEZER DIMENSIONS Product Service Domestic 1-800-522-7658, International 816-333-8811 21
APPENDIX C BENCHTOP SHELL FREEZER ELECTRICAL SPECIFICATIONS This Appendix contains technical information about the Freeze Dryer including electrical specifications and environmental operating conditions. Electrical Specifications Catalog Operating Number Nominal Range Frequency Phase Amperage 7949020 115 103-127 60 1 6 100 90-110 50 1 6 7949030 230 198-254 50 1 2.5 7949040 230 187-253 60 1 2.5 7949060 230 187-253 60 1 2.5 7949070 230 187-253 60 1 2.5 Environmental Conditions • Indoor use only.
Appendix C: Benchtop Shell Freezer Specifications • Used in an environment of Pollution degrees 2 (i.e., where normally only non-conductive atmospheres are present). Occasionally, however, a temporary conductivity caused by condensation must be expected, in accordance with IEC 664.
24 Product Service Domestic 1-800-522-7658, International 816-333-8811
