User’s Manual CentriVap® Refrigerated Centrifugal Concentrators Models 7310000 Series To receive important product updates, complete your product registration card online at register.labconco.com Labconco Corporation 8811 Prospect Avenue Kansas City, MO 64132-2696 800-821-5525, 816-333-8811 Fax 816-363-0130 E-MAIL labconco@labconco.com HOME PAGE www.labconco.com Please read the User’s Manual before operating the equipment.
Copyright © 2005, 2007, 2011, 2013 Labconco Corporation. All rights reserved. The information contained in this manual and the accompanying products are copyrighted and all rights reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change. Warranty Labconco provides a warranty on all parts and factory workmanship.
TABLE OF CONTENTS CHAPTER 1: INTRODUCTION Safety Symbols 1 2 CHAPTER 2: PREREQUISITES Electrical Requirements Location and Exhaust Requirements Vacuum Pump Requirements Vacuum Line Traps Space Requirements 3 4 4 4 5 5 CHAPTER 3: GETTING STARTED Unpacking Your CentriVap CentriVap Components Emergency Access Into the Chamber Component Orientation & Hose Connections Electrical Connection Rotor Installation Chemical Resistance Chart Solvent Safety Precautions 6 7 7 8 9 10 10 11 12 CHAPTER 4: USING YOUR CE
CHAPTER 5: MAINTAINING YOUR CENTRIVAP 20 CHAPTER 6: ACCESSORIES FOR YOUR CENTRIVAP Installing a Glass Lid Installing a Vacuum Gauge Installing a CentriZap Strobe Light 22 24 24 25 CHAPTER 7: TROUBLESHOOTING 26 APPENDIX A: CENTRIVAP COMPONENTS 30 APPENDIX B: CENTRIVAP DIMENSIONS 32 APPENDIX C: CENTRIVAP SPECIFICATIONS Electrical Specifications Environmental Conditions Evaporation Rates 33 33 34 35 DECLARATION OF CONFORMITY 36
Chapter 1: Introduction Congratulations on your purchase of a Labconco CentriVap Refrigerated Concentrator. Models are available for operation on 115V or 230V. The CentriVap Refrigerated Concentrator, when combined with the CentriVap Cold Trap, uses centrifugal force and vacuum with or without heat to rapidly evaporate and condense solvents from biological and analytical samples. Centrifugation eliminates bumping and foaming as vacuum is applied and also concentrates the solute in the bottom of the vial.
Chapter 1: Introduction Safety Symbols Your Refrigerated CentriVap Concentrator was designed with safety in mind, however conditions may exist that could be hazardous. Throughout this manual potentially hazardous conditions are identified using the following words and symbols. It is important that you understand the warnings listed throughout this manual before you operate the CentriVap Concentrator.
Chapter 2: Prerequisites Before you install your CentriVap Refrigerated Concentrator, you need to prepare your site for installation. You must be certain that the area is level and of solid construction. In addition, a means to exhaust the vacuum pump must be provided. An electrical source must be located near the installation site. Carefully read this chapter to learn: • The electrical supply requirements. • The exhaust requirements. • The vacuum pump requirements.
Chapter 2: Prerequisites Electrical Requirements The CentriVap Refrigerated Concentrator requires a dedicated grounded electrical outlet. This outlet requires a 20 Amp circuit breaker or fuse for models rated at 115V (60 Hz). An 8 Amp circuit breaker or fuse is required for models rated at 230V (50/60 Hz). 115V models are equipped with a 20 Amp NEMA 5-20P plug.
Chapter 2: Prerequisites Vacuum pumps used with 115V models should be equipped with a 115V, 15 Amp NEMA 5-15P plug. Vacuum pumps used with 230V models should be equipped with a “reverse” IEC 320 plug. This will allow the vacuum pump to be plugged into the receptacle on the back of the CentriVap. IMPORTANT NOTE: To ensure that aggressive samples used in the CentriVap Refrigerated Concentrator do not damage the vacuum pump, it is recommended that all internal wetted parts are PTFE or PTFE coated.
Chapter 3: Getting Started Now that the site for your CentriVap is properly prepared, you are ready to unpack, inspect, install, and test your CentriVap. Read this chapter to learn how to: • Unpack and move your CentriVap. • Set up your CentriVap. • Connect the electrical supply source to your CentriVap. • Properly exhaust your CentriVap. • Safely use solvents with your CentriVap. CAUTION: The CentriVap Refrigerated Concentrator weighs over 95 lbs. (43 Kg).
Chapter 3: Getting Started Unpacking Your CentriVap Refrigerated Concentrator Carefully unpack your CentriVap and inspect it for damage that may have occurred in transit. If your CentriVap is damaged, notify the delivery carrier immediately and retain the entire shipment intact for inspection by the carrier. The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen (15) days of delivery.
Chapter 3: Getting Started Plus the following: Part # Component Description 7310605 User’s Manual 1336400 Power Cord – 115V – US or 1332700 Power Cord – 230V – China or 1332600 Power Cord – 230V – UK or 1338000 Power Cord – 230V – US or 1336100 Power Cord – 230V – EU 7828606 Tubing 1488800 Clamp (2) 7397601 Coupling Insert or 7396206 Coupling Inert – Acid Resistant If you do not receive one or more of the components listed for your CentriVap, contact Labconco Corporation immediately for further instruction
Chapter 3: Getting Started Component Orientation & Hose Connections The relative position of the CentriVap, Cold Trap and Vacuum Pump should be as shown to optimize the air flows of the refrigeration systems. There must be at least 6 inches between the Refrigerated CentriVap and the Cold Trap. More space is better. There must be at least 3 inches between the back of the refrigerated CentriVap and the adjacent wall surface.
Chapter 3: Getting Started Electrical Connection Plug the power cord into the receptacle on the back of the CentriVap Concentrator and plug the other end into a suitable power receptacle. Plug the power cord into the receptacle on the back of the CentriVap Cold Trap and plug the other end into a suitable power receptacle. Plug the power cord from the vacuum pump into the receptacle on the back of the Concentrator. If the vacuum pump has an off/on switch, turn the switch ON.
Chapter 3: Getting Started Rotor Rotor Shaft & Cold Trap Bearings Anodized Aluminum Stainless Steel High Carbon Steel C D C C D D D D D C D D D D D D D C D D D D D D D D D C D D C D C D C Water Toluene Methylene Chloride D D D D D D C D D D D D D D D C D D D D D D C-Moderate Degradation - Questionable use D-Severe Degradation - Infrequent use recommended - immediate thorough neutralizing and cleaning is required.
Chapter 3: Getting Started • Drain the Cold Trap immediately after the collected ice is melted to prevent corrosive liquids from residing in the trap. Flush out the trap with water after draining. DO NOT chip ice off the Cold Trap walls as damage may occur. DO NOT start a rotary vane pump when the Cold Trap contains any liquid. The liquid will be drawn into the pump and will contaminate the vacuum pump oil. • If the compounds used attack acrylic, consider using the optional glass chamber lid.
Chapter 3: Getting Started CAUTION: Solvents used in the CentriVap may be flammable or hazardous. Use extreme caution and keep sources of ignition away from the solvents. When using flammable or hazardous solvents, both the CentriVap and the vacuum pump should be operated inside a fume hood. If a sample is spilled in the chamber it must immediately be cleaned up.
Chapter 4: Using Your CentriVap Refrigerated Concentrator After your CentriVap has been installed as detailed in Chapter 3: Getting Started, you are ready to begin using your CentriVap. Read this chapter to learn how to: • Set operating parameters. • Operate the controls. • Properly select and position glassware inside your CentriVap. • Understand the display. • Interrupt a cycle after it has begun.
Chapter 4: Using Your CentriVap Refrigerated Concentrator Glassware Selection Normally, sample tubes should be filled no more than approximately half full. Select the size of the sample tube so it is compatible with the rotor and the desired sample size. Tubes should not be excessively loose in the rotor. Rotors are available with holes for various size tubes. Refer to Chapter 6: Accessories for Your CentriVap for available rotor sizes.
Chapter 4: Using Your CentriVap Refrigerated Concentrator 1. Display – The liquid crystal display (LCD) shows set point parameters and actual measured conditions. 2. Program Buttons – Used to initiate the start of a run with the use of just one button. 3. Run/Stop Button – Press this to start or stop a run. 4. Preheat/Cool Button – Used to turn on the heater or refrigeration system to heat or cool the chamber prior to loading samples. 5.
Chapter 4: Using Your CentriVap Refrigerated Concentrator Change “Run Time” set points: To change the time set points, press the set point “SELECT” button until arrows point to the “Time at 1” or “Time at 2” set point which can be changed from 1 to 999 minutes by pressing the increase or decrease buttons. If it is desired to have the CentriVap run continuously without alarming at the end of a time period, press the increase button until the “Time” display says “ON.
Chapter 4: Using Your CentriVap Refrigerated Concentrator 6. Load the rotor with samples into the chamber. 7. Close the lid. A safety switch prevents the CentriVap from starting when the lid is open. 8. Press “RUN.” If the display was showing set point parameters, it will change to show actual parameters. Press “RUN” again. The “S” (STOP) in the display changes to “R” (RUN). The program on the display alternately displays “R” or the number of the program that is running.
Chapter 4: Using Your CentriVap Refrigerated Concentrator Safety Precautions Special precautions must be observed if the materials used in the CentriVap Concentrator are known to be hazardous, toxic, radioactive, or contaminated with pathogenic micro organisms. These actions should include but are not limited to the following: • Refer to the World Health Organization Laboratory Biosafety Manual, paying special attention to information about centrifuges and the handling of hazardous materials.
Chapter 5: Maintaining Your CentriVap Refrigerated Concentrator Under normal operation, the CentriVap requires little maintenance. The following maintenance schedule is recommended. Before servicing the CentriVap Refrigerated Concentrator, disconnect electrical power. Special precautions must be observed if materials used in the CentriVap Concentrator are known to be hazardous, toxic, radioactive or contaminated with pathogenic micro organisms.
Chapter 5: Maintaining Your CentriVap Refrigerated Concentrator 7. If the Glass Trap is used, check to see that the ethanol in the stainless steel trap is free of ice or water. Drain the ethanol and replace it with fresh ethanol. 8. If the media in the cartridge in the optional clear canister has changed color, discard and replace the insert with a new insert. For the radiochemical trap insert, no indicator exists; therefore, it should be discarded after each use.
Chapter 6: Accessories for Your CentriVap The configuration of your CentriVap can be changed to accommodate your needs. If the solvents used in the CentriVap degrade the acrylic lid, it may be replaced with an optional glass lid to gain added chemical resistance. To observe the samples while they are processing, an optional CentriZap Strobe light may be installed. Read this chapter to learn how to: 22 • Install a glass lid • Install a vacuum gauge. • Install a CentriZap™ strobe light.
Chapter 6: Accessories for Your CentriVap The following accessories are available for the CentriVap Concentrator and Cold Trap System. PART # 7462900 7462901* DESCRIPTION Rotor (DNA) Holds (72) 0.5 ml microcentrifuge tubes and (60) 1.5 ml microcentrifuge tubes or (60) 2.0 ml microcentrifuge tubes 7450700 7450701* Rotor (1.5 ml) Holds (132) 1.5 ml microcentrifuge tubes or (132) 2.
Chapter 6: Accessories for Your CentriVap 1467700 1472100 7739402 7739403 1473400 1473200 1473300 1988000 7393000 7393001 7539902 7584000 7584002 7456600 7464300 Vacuum Pump – 195 liters/minute capacity with gas ballast. Ultimate pressure 1.3 x 10-4 mBar. 115 VAC, 60 Hz, single phase, 7.8 amp. Vacuum Pump – 117 liters/minute pumping capacity with gas ballast. Ultimate pressure 1.3 x 10-4 mBar. 115 VAC, 60 Hz, single phase, 4.6 amp. Vacuum Pump – 117 liters/minute.
Chapter 6: Accessories for Your CentriVap Installing a CentriZap™ Strobe Light An accessory strobe light is available to enable you to see the samples as they are rotating in the rotor. Attach the holder to the right hand side of the CentriVap Concentrator using the screws provided. Plug the connector on the strobe light harness into the receptacle on the back of the CentriVap marked “STROBE OUTLET.” Peel the protective backing off the enclosed Velcro® and attach it to the back of the Concentrator.
Chapter 7: Troubleshooting Refer to the following if your CentriVap fails to operate properly. If the suggested corrective actions do not solve your problem, contact Labconco for additional assistance. The following failure codes may appear on the display when problems are sensed by the internal self check system. CAUTION: Disconnect power before servicing the CentriVap. DISPLAY ERROR CODE Heat Sensor CAUSE CORRECTIVE ACTION Sensor failure Replace sensor assembly.
Chapter 7: Troubleshooting Other corrective actions for potential problems are as follows: PROBLEM Unit will not operate CAUSE CORRECTIVE ACTION Unit not connected to electrical power Connect unit to proper electrical receptacle. Circuit breaker blown Correct electrical problem and reset circuit breaker by pressing button. Lid open Close lid. Excessive vibration Sample tubes not located symmetrically in rotor Reposition sample tubes.
Chapter 7: Troubleshooting PROBLEM Frequent oil change needed in pump Unit starts and shuts off 28 CAUSE CORRECTIVE ACTION Secondary Trap insert is spent Change insert often. Cold Trap is not emptied after each run and dried Empty the traps (glass or stainless steel) after each run and replace. Vacuum too strong for chemical Use a Secondary Trap insert and diaphragm pump. Latch optical sensor improperly calibrated Unplug power cord. Wait 10 seconds. Plug in power cord.
Chapter 7: Troubleshooting Product Service 1-800-522-7658 29
Appendix A: CentriVap Refrigerated Concentrator Components The following pages list components that are available for your CentriVap. The parts shown are the most common replacement parts. If other parts are required, contact Product Service. Item Quantity Part No. 1 2 2A 2B 3 3A 4 5 6 7 8 9 9A 10 11 12 13 14 15 15A 16 17 18 18A 19 19A 20 20A 20B 21 Not Shown Not Shown Not Shown Not Shown Not Shown 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 42.
Appendix A: CentriVap Refrigerated Concentrator Components Product Service 1-800-522-7658 31
Appendix B: CentriVap Refrigerated Concentrator Dimensions 32 Product Service 1-800-522-7658
Appendix C: CentriVap Refrigerated Concentrator Specifications This Appendix contains technical information about the CentriVap including specifications, environmental operating conditions, and evaporation rates. Electrical Specifications • Nominal amperage for 115V CentriVap Concentrator (model 7310020 series) (excluding vacuum pump): 3.1 amps (with vacuum pump) 12.00A max. • Nominal amperage for 230V CentriVap Concentrator (model 7310030 & 7310040 series) (excluding vacuum pump): 1.
Appendix C: CentriVap Refrigerated Concentrator Specifications Environmental Conditions • Indoor use only. 34 • Maximum altitude: 6562 feet (2000 meters). • Ambient temperature range: 41° to 104°F (5° to 40°C). • Maximum relative humidity: 80% for temperatures up to 88°F (31°C), decreasing linearly to 50% relative humidity at 104°F (40°C). • Main supply voltage fluctuations not to exceed ±10% of the nominal voltage.
Appendix C: CentriVap Refrigerated Concentrator Specifications Evaporation Rates Tube Size (ml) Methylene Chloride 50 15 Number of Samples Sample Size (ml) Heater Temp (C) Vacuum Pump Time to Dry (min) Cold Trap Rate Overall (ml/min) bp 40 12 18 25 10 45 45 Diaphragm Diaphragm 80 45 no no 3.75 4.00 25 25 10 10 45 100 45 100 Diaphragm Diaphragm Diaphragm Diaphragm 204 96 119 42 no no no no 1.47 3.13 1.51 4.
36 Product Service 1-800-522-7658
