User’s Manual CentriVap® Mobile Systems Models 7812010 7812011 7812012 7812013 7812014 7812015 To receive important product updates, complete your product registration card online at register.labconco.com Labconco Corporation 8811 Prospect Avenue Kansas City, MO 64132-2696 800-821-5525, 816-333-8811 Fax 816-363-0130 E-MAIL labconco@labconco.com HOME PAGE www.labconco.com Please read the User’s Manual before operating the equipment.
Copyright © 2005, 2007, 2013 Labconco Corporation. All rights reserved. The information contained in this manual and the accompanying products are copyrighted and all rights reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change. Warranty Labconco provides a warranty on all parts and factory workmanship.
TABLE OF CONTENTS CHAPTER 1: INTRODUCTION Safety Symbols 1 2 CHAPTER 2: PREREQUISITES Electrical Requirements Location and Exhaust Requirements Vacuum Pump Requirements Vacuum Line Traps Space Requirements 3 3 4 4 5 5 CHAPTER 3: GETTING STARTED Unpacking Your CentriVap CentriVap Components Setting Up Your CentriVap Emergency Access Into the Chamber Vacuum Pump Installation Exhaust Port Installing a Secondary Chemical Trap Electrical Connection Rotor Installation Ground Wire Chemical Resistance Chart Sol
Operational Notes Interrupting a Cycle After it Has Begun Safety Precautions 19 19 20 CHAPTER 5: MAINTAINING YOUR CENTRIVAP 21 CHAPTER 6: ACCESSORIES FOR YOUR CENTRIVAP Installing a Vacuum Gauge Installing a CentriZap Strobe Light Installing a Glass Trap in the Cold Trap 23 26 27 28 CHAPTER 7: TROUBLESHOOTING 29 APPENDIX A: CENTRIVAP COMPONENTS 32 APPENDIX B: CENTRIVAP DIMENSIONS 36 APPENDIX C: CENTRIVAP SPECIFICATIONS Electrical Specifications Environmental Conditions Wiring Diagram Concentrato
Chapter 1: Introduction Congratulations on your purchase of a Labconco CentriVap Mobile System. Models are available for operation on 115V or 230V. The Mobile System combines a concentrator and a cold trap in one cabinet. The concentrator uses centrifugal force with heat and vacuum to rapidly evaporate and condense solvents from biological and analytical samples. Centrifugation eliminates bumping and foaming as vacuum is applied and also concentrates the solute in the bottom of the vial.
Chapter 1: Introduction Safety Symbols Your CentriVap Concentrator was designed with safety in mind, however conditions may exist that could be hazardous. Throughout this manual potentially hazardous conditions are identified using the following words and symbols. It is important that you understand the warnings listed throughout this manual before you operate the CentriVap Concentrator.
Chapter 2: Prerequisites Before you install your CentriVap, you need to prepare your site for installation. Carefully examine the location where you intend to install your CentriVap. You must be certain that the area is level and of solid construction. In addition, an exhaust means must be provided. An electrical source must be located near the installation site. Carefully read this chapter to learn: • The electrical supply requirements. • The exhaust requirements. • The vacuum pump requirements.
Chapter 2: Prerequisites Location and Exhaust Requirements The CentriVap Concentrator should be located on a surface that is stable, flat and level. WARNING: If hazardous or flammable solvents are used, it is recommended that a benchtop CentriVap Concentrator is used and that it is located inside a fume hood. Heating of materials could lead to the liberation of hazardous gases. In all cases, regardless of the solvent used, it is strongly recommended that the vacuum pump is vented in a fume hood.
Chapter 2: Prerequisites Vacuum Line Traps When evaporating acids, it is advisable to include a soda lime acid secondary trap in the vacuum line. This adds additional protection for the pump. NOTE: Several components within the CentriVap are made from stainless steel or aluminum and can be degraded if exposed to acids. Before evaporating acids, be sure to contact Labconco. A moisture trap, solvent trap and radioisotope trap are also available.
Chapter 3: Getting Started Now that the site for your CentriVap Mobile System is properly prepared, you are ready to unpack, inspect, install, and test your CentriVap Mobile System. Read this chapter to learn how to: • Unpack and move your CentriVap Mobile System. • Set up your CentriVap Mobile System. • Connect the electrical supply source to your CentriVap Mobile System. • Properly exhaust your CentriVap Mobile System. • Safely use solvents with your CentriVap Mobile System.
Chapter 3: Getting Started Unpacking Your CentriVap Carefully unpack your CentriVap and inspect it for damage that may have occurred in transit. If your CentriVap is damaged, notify the delivery carrier immediately and retain the entire shipment intact for inspection by the carrier. The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen (15) days of delivery. NOTE: Do not return goods without the prior authorization of Labconco.
Chapter 3: Getting Started Setting Up Your CentriVap After you verify receipt of the proper components, move your CentriVap to the level and sturdy location where you want to install it. There is no need to secure the CentriVap to the work surface. Then, follow the steps listed below. Emergency Access Into the Chamber The CentriVap is designed to prevent access to the chamber in the event of a power disruption.
Chapter 3: Getting Started Vacuum Pump Installation The user-supplied vacuum pump can be installed inside the cabinet of the CentriVap Mobile Console. To gain access to the pump area, grasp the front lower panel near the top and pull it outward. The vacuum pump should be positioned on the lower shelf of the cabinet. Attach the hose that is hanging from the clear canister to the fitting of the vacuum pump. Clamp the hose securely with the clamp supplied.
Chapter 3: Getting Started Exhaust Port Attach one end of a user-supplied hose to the exhaust port on the vacuum pump. Clamp securely. Route the other end to a fume hood or other laboratory ventilation device. Installing a Secondary Chemical Trap Insert A secondary chemical trap is included to minimize the exhausting of solvents into the atmosphere. Unscrew the clear bowl of the canister housing from the head.
Chapter 3: Getting Started Ground Wire CAUTION: When draining the CentriVap Cold Trap always attach the grounding clip to the solvent catch pot to eliminate the risk of electrostatic spark ignition. Chemical Resistance of CentriVap Components Your CentriVap Mobile System is designed to be chemical resistant to most compounds that are commonly used in the concentration processes that are performed in it.
Chapter 3: Getting Started • If a rotary vane vacuum pump is used, most compounds used in the CentriVap will degrade the oil if allowed to enter to pump. Frequent oil changes are required. • Diaphragm vacuum pumps sold by Labconco have wetted parts either made from PTFE or protected by PTFE coatings and are suitable for nearly all procedures. When using compounds in the CentriVap that are hostile to the materials of construction, it is imperative that the equipment is appropriately maintained.
Chapter 3: Getting Started Solvent Safety Precautions CAUTION: The CentriVap is not classified as “explosion proof.” It has been designed with safety as a primary consideration and should be used in a prudent manner using “good laboratory practices.” It has been designed for use with compounds as described in the United States National Electrical Code Class I, Group D. The heater may be programmed to run as hot as 100°C, however, the heater element may normally run at 110°C.
Chapter 4: Using Your CentriVap Mobile System After your CentriVap Mobile System has been installed as detailed in Chapter 3: Getting Started, you are ready to begin using your CentriVap. Read this chapter to learn how to: • Set operating parameters. • Operate the controls. • Properly select and position glassware inside your CentriVap. • Understand the display. • Interrupt a cycle after it has begun. NOTE: See Appendix C: CentriVap Specifications for electrical requirements.
Chapter 4: Using Your CentriVap Mobile System Planning Thoroughly understand procedures and the equipment operation prior to beginning work. The unique performance of the CentriVap is dependent upon the proper balance of heat, vacuum and centrifugal force. If the proper balance is not established, it is possible to damage or lose a portion of the sample.
Chapter 4: Using Your CentriVap Mobile System The tables in Appendix C: CentriVap Specifications indicate approximate times required to evaporate various common solvents. Actual times must be determined by the user. The CentriVap can be set to alarm after a preset period of operation. When the time expires, the CentriVap will give an audible alarm and turn itself off.
Chapter 4: Using Your CentriVap Mobile System 7. Set Point Select Button – To select a parameter to change, press the select button. Arrows on the display will point to the parameter that may be altered. 8. Heat Boost Button – If the model is so equipped, pressing the button will activate or deactivate the auxiliary Heat Boost heater. 9. Cold Trap Button – Used to start or stop the refrigeration system for the cold trap.
Chapter 4: Using Your CentriVap Mobile System If during a run the “STOP” button is pressed, the timers remember the time at which stop occurred. If “RUN” is then pressed, the timers continue to count down from the time at which they were stopped. If you are running program 1, program 2 or program 3, pressing “PROG 1,” “PROG 2” or “PROG 3” resets the timers to the original set point time and the CentriVap starts a new run.
Chapter 4: Using Your CentriVap Mobile System 9. Press “RUN.” If the display was showing set point parameters, it will change to show actual parameters. Press “RUN” again. The “S” (STOP) in the display changes to “R” (RUN). The program on the display alternately displays “R” or the number of the program that is running. If you intend to run program 1, 2 or 3, simply press “PROG 1,” “PROG 2” or “PROG 3” to quick start the CentriVap.
Chapter 4: Using Your CentriVap Mobile System Safety Precautions Special precautions must be observed if the materials used in the CentriVap Concentrator are known to be hazardous, toxic, radioactive, or contaminated with pathogenic micro organisms. These actions should include but are not limited to the following: • Refer to the World Health Organization Laboratory Biosafety Manual, paying special attention to information about centrifuges and the handling of hazardous materials.
Chapter 5: Maintaining Your CentriVap Mobile System Under normal operation, the CentriVap Mobile System requires little maintenance. The following maintenance schedule is recommended. Before servicing the CentriVap Mobile System, disconnect electrical power. Special precautions must be observed if materials used in the CentriVap Concentrator are known to be hazardous, toxic, radioactive or contaminated with pathogenic micro organisms.
Chapter 5: Maintaining Your CentriVap Mobile System NOTE: If the ice has melted, the glass trap insert must be emptied before the Cold Trap is started again. 7. If the Glass Trap is used, check to see that the ethanol in the stainless steel trap is free of ice or water. Drain the ethanol and replace it with fresh ethanol. 8. If the media in the optional clear canister has changed color, discard and replace the insert with a new insert.
Chapter 6: Accessories for Your Centrivap Mobile System The configuration of your CentriVap Mobile System can be changed to accommodate your needs. If the solvents used in the CentriVap degrade the acrylic lid, it may be replaced with an optional glass lid to gain added chemical resistance. To observe the samples while they are processing, an optional CentriZap Strobe light may be installed. Read this chapter to learn how to: • Install a vacuum gauge. • Install a CentriZap™ strobe.
Chapter 6: Accessories for Your CentriVap Mobile System The following accessories are available for the CentriVap Concentrator and Cold Trap System. PART # 7462900 7462901* 7450700 7450701* 7455000 7455001* DESCRIPTION Rotor (DNA) Holds (72) 0.5 ml microcentrifuge tubes and (60) 1.5 ml microcentrifuge tubes or (60) 2.0 ml microcentrifuge tubes Rotor (1.5 ml) Holds (132) 1.5 ml microcentrifuge tubes or (132) 2.
Chapter 6: Accessories for Your CentriVap Mobile System 7995600 Ammonia Trap Insert 7815000 Radiochemical Trap Insert 7815200 7464300 Solvent Trap Insert CentriZap™ Strobe Light – For observing samples while the rotor is spinning. 1472100 Direct Drive Vacuum Pump – 117 liters/minute pumping capacity with gas ballast. Ultimate pressure 1.3 x 10-4 mBar. 115 VAC, 60 Hz, single phase, 4.6 amp. Direct Drive Vacuum Pump – 195 liters/minute capacity with gas ballast. Ultimate pressure 1.3 x 10-4 mBar.
Chapter 6: Accessories for Your CentriVap Mobile System 7772700 7438700 7438800 7769600 7769800 7439000 7439200 Vacuum Pump Oil 1 Liter – A mineral oil with low vapor pressure. For vacuum pumps 7439000, 7439200, 7769600 and 7769800 Vacuum Pump – Two stage direct drive pump, 108 liters/minute. 115 VAC 50/60 Hz, single phase. Includes Pump Exhaust Filter. (Catalog #7670400) Vacuum Pump - Two stage direct drive pump, 108 liters/minute. 230 VAC 50/60 Hz, single phase. Includes Pump Exhaust Filter.
Chapter 6: Accessories for Your CentriVap Mobile System Installing a CentriZap™ Strobe Light An accessory strobe light is available to enable you to see the samples as they are rotating in the rotor. Attach the holder to the right hand side of the CentriVap Concentrator using the screws provided. Plug the connector on the strobe light harness into the receptacle on the back of the CentriVap marked “STROBE OUTLET.
Chapter 6: Accessories for Your CentriVap Mobile System Installing a Glass Trap in the Cold Trap An accessory Glass Trap is available for use in the Cold Trap for use when corrosive chemicals are used that could attack the stainless steel chamber of the Cold Trap. Lift and rotate the two lid latches. Disconnect the hose and remove the Cold Trap Lid Assembly. Attach the hose from the Concentrator to the fitting on the center tube of the Glass Trap.
Chapter 7: Troubleshooting Refer to the following if your CentriVap fails to operate properly. If the suggested corrective actions do not solve your problem, contact Labconco for additional assistance. The following failure codes may appear on the display when problems are sensed by the internal self check routine. CAUTION: Disconnect power before corrective action is taken. DISPLAY ERROR CODE CAUSE CORRECTIVE ACTION Heat Sensor Sensor failure Replace sensor assembly.
Chapter 7: Troubleshooting Other corrective actions for potential problems are as follows: PROBLEM Unit will not operate CAUSE CORRECTIVE ACTION Unit not connected to electrical power Connect unit to proper electrical receptacle. Circuit breaker blown Correct electrical problem and reset circuit breaker by pressing button. Lid open Close lid. Excessive vibration Sample tubes not located symmetrically in rotor Reposition sample tubes.
Chapter 7: Troubleshooting PROBLEM No vacuum/poor vacuum (cont.) Recovery of condensate in Cold Trap is less than normally expected CAUSE Ice formed on Cold Trap lid sealing surface CORRECTIVE ACTION Defrost and wipe dry. Secondary Trap Cannister not fitted properly Tighten all connections to and from the Secondary Trap Cannister. Secondary Trap Insert is spent Replace with new insert. New Secondary Trap has moisture in it Run vacuum pump for 24 hours to remove moisture.
Appendix A: CentriVap Components The following pages list components that are available for your CentriVap. The parts shown are the most common replacement parts. If other parts are required, contact Product Service. CentriVap Concentrator Components Item 1 1A 2 2A 3 4 5 6 8 9 10 11 12 13 14 15 16 16A 17 18 19 20 21 22 22A 23 24 25 26 26A Not Shown Not Shown 32 Quantity 1 1 1 1 1 1 1 2 1 1 1 1 1 1 1 1 1 1 42.2 inch 1 1 1 1 1 1 1 1 1 1 2 1 1 Part No.
Appendix A: CentriVap Components Product Service 1-800-522-7658 33
Appendix A: CentriVap Components CentriVap Cold Trap Components Item Quantity Part No.
Appendix A: CentriVap Components 10 11 9 15 & 16 14 4 2 Product Service 1-800-522-7658 35
Appendix B: CentriVap Dimensions CentriVap Concentrator 36 Product Service 1-800-522-7658
Appendix C CentriVap Specifications This Appendix contains technical information about the CentriVap including specifications, environmental operating conditions, wiring diagrams and evaporation rates. Electrical Specifications • Nominal amperage for 115V/60 Hz CentriVap (model 7812010) (excluding vacuum pump): 10A. • Nominal amperage for 230V/50Hz CentriVap (model 7812011) (excluding vacuum pump): 4.7A. • Nominal amperage for 230V/60Hz CentriVap (model 7812012) (excluding vacuum pump): 4.7A.
Appendix C: CentriVap Specifications Environmental Conditions • Indoor use only. • Maximum altitude: 6562 feet (2000 meters). • Ambient temperature range: 41° to 104°F (5° to 40°C). • Maximum relative humidity: 80% for temperatures up to 88°F (31°C), decreasing linearly to 50% relative humidity at 104°F (40°C). • Main supply voltage fluctuations not to exceed ±10% of the nominal voltage. • Transient overvoltages according to Installation Categories II (Overvoltage Categories per IEC 1010).
Appendix C: CentriVap Specifications Wiring Diagram Concentrator (115V Model) (Models 7812010 and 7812013) Product Service 1-800-522-7658 39
Appendix C: CentriVap Specifications Wiring Diagram Concentrator (230V Model) (Models 7812011, 7812012 7812014 and 7812015) 40 Product Service 1-800-522-7658
Appendix C: CentriVap Specifications Evaporation Rates Methylene Chloride bp 40 Tube Size (ml) Number of Samples Sample Size (ml) Heater Temp (C) Heat Boost Vacuum Pump Time to Dry (min) Cold Trap Rate Overall (ml/min) 50 50 15 15 12 12 18 18 25 25 10 10 45 45 45 45 off on off on Diaphragm Diaphragm Diaphragm Diaphragm 80 78 45 45 no no no no 3.75 3.85 4.00 4.
Appendix C: CentriVap Specifications bp 100 Water Tube Size (ml) Number of Samples Sample Size (ml) Heater Temp (C) Heat Boost Vacuum Pump Time to Dry (min) Cold Trap Rate Overall (ml/min) 50 50 50 15 1.5 1.5 1.5 1.5 1.
Product Service 1-800-522-7658 43
