User’s Manual CentriVap® DNA Systems Models 7970010 7970011 7970030 7970035 7970037 To receive important product updates, complete your product registration card online at register.labconco.com Labconco Corporation 8811 Prospect Avenue Kansas City, MO 64132-2696 800-821-5525, 816-333-8811 FAX 816-363-0130 E-MAIL labconco@labconco.com HOME PAGE www.labconco.com Please read the User’s Manual before operating equipment.
Copyright © 2009, 2012, 2013 Labconco Corporation. All rights reserved. The information contained in this manual and the accompanying products are copyrighted and all rights reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design changes without obligation to notify any person or entity of such change. Warranty Labconco provides a warranty on all parts and factory workmanship.
TABLE OF CONTENTS CHAPTER 1: INTRODUCTION Safety Symbols 1 2 CHAPTER 2: PREREQUISITES Electrical Requirements Location and Exhaust Requirements Space Requirements 3 3 4 4 CHAPTER 3: GETTING STARTED Unpacking Your CentriVap CentriVap Components Setting Up Your CentriVap Emergency Access Into the Chamber Exhaust Port Electrical Connection Rotor Installation Chemical Resistance of CentriVap Components Solvent Safety Precautions 5 6 6 7 7 7 7 7 8 9 CHAPTER 4: USING YOUR CENTRIVAP Planning Glassware Select
CHAPTER 6: ACCESSORIES FOR YOUR CENTRIVAP Installing a Glass Lid Installing a Secondary Chemical Trap Installing a Vacuum Gauge Installing a CentriZap Strobe Light 20 22 22 23 24 CHAPTER 7: TROUBLESHOOTING 25 APPENDIX A: CENTRIVAP COMPONENTS 27 APPENDIX B: CENTRIVAP DIMENSIONS 29 APPENDIX C: CENTRIVAP SPECIFICATIONS Electrical Specifications Environmental Conditions Evaporation Rates 30 30 30 31 DECLARATION OF CONFORMITY 32
Chapter 1: Introduction Congratulations on your purchase of a Labconco CentriVap DNA Centrifugal Concentrator . Models are available for operation on 115V or 230V. The CentriVap Concentrator uses centrifugal force with heat and vacuum to rapidly dry precipitates of DNA and RNA samples. Centrifugation eliminates bumping and foaming as vacuum is applied and also concentrates the solute in the bottom of the vial. This allows recovery of solutes from sample volumes as small as a few microliters.
Chapter 1: Introduction Safety Symbols Your CentriVap Concentrator was designed with safety in mind, however conditions may exist that could be hazardous. Throughout this manual potentially hazardous conditions are identified using the following words and symbols. It is important that you understand the warnings listed throughout this manual before you operate the CentriVap Concentrator.
Chapter 2: Prerequisites Before you install your CentriVap, you need to prepare your site for installation. Carefully examine the location where you intend to install your CentriVap. You must be certain that the area is level and of solid construction. In addition, an exhaust means must be provided. An electrical source must be located near the installation site. Carefully read this chapter to learn: • The electrical supply requirements. • The exhaust requirements.
Chapter 2: Prerequisites Location and Exhaust Requirements The CentriVap Concentrator should be located on a surface that is stable, flat and level. WARNING: The CentriVap System should be located within a fume hood if hazardous or flammable solvents are used. Heating of materials could lead to the liberation of hazardous gases. In all cases, regardless of the solvent used, it is strongly recommended that the vacuum pump is vented in a fume hood.
Chapter 3: Getting Started Now that the site for your CentriVap is properly prepared, you are ready to unpack, inspect, install, and test your CentriVap. Read this chapter to learn how to: • Unpack and move your CentriVap. • Set up your CentriVap. • Connect the electrical supply source to your CentriVap. • Properly exhaust your CentriVap. • Safely use solvents with your CentriVap. CAUTION: The CentriVap Concentrator weighs over 80 lbs. (36 Kg).
Chapter 3: Getting Started Unpacking Your CentriVap Carefully unpack your CentriVap and inspect it for damage that may have occurred in transit. If your CentriVap is damaged, notify the delivery carrier immediately and retain the entire shipment intact for inspection by the carrier. The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen (15) days of delivery. NOTE: Do not return goods without the prior authorization of Labconco.
Chapter 3: Getting Started Setting Up Your CentriVap After you verify receipt of the proper components, move your CentriVap to the level and sturdy location where you want to install it. There is no need to secure the CentriVap DNA Concentrator to the work surface. Then, follow the steps listed below. Emergency Access Into the Chamber The CentriVap is designed to prevent access to the chamber in the event of a power disruption.
Chapter 3: Getting Started Chemical Resistance of CentriVap Components Your CentriVap DNA Centrifugal Concentrator is designed to be chemical resistant to most compounds that are commonly used in the concentration processes that are performed in it. However, by necessity, the CentriVap is comprised of a number of different materials, some of which may be attacked and degraded by certain chemicals. The degree of degradation is obviously dependent on the concentration and duration of exposure.
Chapter 3: Getting Started When using compounds in the CentriVap that are hostile to the materials of construction, it is imperative that the equipment is appropriately maintained. • After each run, clean up all residues, spills and materials that might have splashed in the chamber using agents suitable for the substance involved. • If the compounds used attack acrylic, consider using the optional glass chamber lid. Contact Labconco for ordering information.
Chapter 3: Getting Started CAUTION: Solvents used in the CentriVap may be flammable or hazardous. Use extreme caution and keep sources of ignition away from the solvents. When using flammable or hazardous solvents, both the CentriVap and the vacuum pump should be operated inside a fume hood. If a sample is spilled in the chamber it must immediately be cleaned up.
Chapter 4: Using Your CentriVap After your CentriVap has been installed as detailed in Chapter 3: Getting Started, you are ready to begin using your CentriVap. Read this chapter to learn how to: • Set operating parameters. • Operate the controls. • Properly select and position glassware inside your CentriVap. • Understand the display. • Interrupt a cycle after it has begun. NOTE: See Appendix C: CentriVap Specifications, for electrical requirements.
Chapter 4: Using Your CentriVap Planning Thoroughly understand procedures and the equipment operation prior to beginning work. The unique performance of the CentriVap is dependent upon the proper balance of heat, vacuum and centrifugal force. If the proper balance is not established, it is possible to damage or lose a portion of the sample.
Chapter 4: Using Your CentriVap The tables in Appendix C: CentriVap Specifications indicate approximate times required to evaporate various common solvents. Actual times must be determined by the user. The CentriVap can be set to alarm after a preset period of operation. When the time expires, the CentriVap will give an audible alarm and turn itself off. CentriVap Controls The control panel for the CentriVap is shown below with a description about its function. 5 3 2 1 4 7 6 1.
Chapter 4: Using Your CentriVap Operating the CentriVap Controls Preheat: To preheat the chamber, press the “PREHEAT” button. The display will show: SP: XXX ACT: XXX Press the increase or decrease button until the desired set point (SP) is displayed. The actual chamber temperature, (ACT) is displayed to the right. When power is being supplied to the heaters, a bar under the actual temperature will illuminate.
Chapter 4: Using Your CentriVap To reset the timers to the original set point time when running programs other than program 1, program 2 or program 3, press the start button and hold it for five seconds. The display will indicate that the timer is reset. Operating the CentriVap CAUTION: To avoid personnel injury; Do not operate the CentriVap if the lid is scratched or nicked, or shows signs of damage. A damaged lid could fail under vacuum.
Chapter 4: Using Your CentriVap 10. Press “STOP” to terminate operation if the CentriVap has not already stopped itself. 11. When the evaporation is complete, allow the rotor to stop moving, lift the lid and remove the samples. Operational Notes When the CentriVap is turned on, the CentriVap returns to the same mode (“RUN” or “STOP”) that it was in when the power was turned off.
Chapter 4: Using Your CentriVap Safety Precautions Special precautions must be observed if the materials used in the CentriVap Concentrator are known to be hazardous, toxic, radioactive, or contaminated with pathogenic micro organisms. These actions should include but are not limited to the following: • Refer to the World Health Organization Laboratory Biosafety Manual, paying special attention to information about centrifuges and the handling of hazardous materials.
Chapter 5: Maintaining Your CentriVap Under normal operation, the CentriVap requires little maintenance. The following maintenance schedule is recommended. Before servicing the CentriVap, disconnect electrical power. Special precautions must be observed if materials used in the CentriVap Concentrator are known to be hazardous, toxic, radioactive or contaminated with pathogenic micro organisms. Before servicing, the CentriVap Concentrator must be suitably decontaminated.
Chapter 6: Accessories for Your CentriVap Daily: 1. To prolong the life of the DNA vacuum pump and to optimize its performance, it is important to purge all liquid from within it. When the DNA concentration process is completed, open the CentriVap lid and push the rotor switch ON. In ten seconds, the pump will operate and will draw air through the system. The rotor will not turn with the lid open. The length of the liquid purge time is dependent on the volatility of the solvents used.
Chapter 6: Accessories for Your CentriVap The configuration of your CentriVap can be changed to accommodate your needs. If the solvents used in the CentriVap degrade the acrylic lid, it may be replaced with an optional glass lid to gain added chemical resistance. You may wish to add a secondary trap to trap vapors exhausted from the vacuum pump. To observe the samples while they are processing, an optional CentriZap Strobe light may be installed.
Chapter 6: Accessories for Your CentriVap The following accessories are available for the CentriVap DNA Concentrator. PART # 7462900 7462901* 7450700 7450701* 7455000 7455001* 7455100 7455101* 7455200 7455201* 7461900 7461901* DESCRIPTION Rotor (DNA) Holds (72) 0.5 ml microcentrifuge tubes and (60) 1.5 ml microcentrifuge tubes or (60) 2.0 ml microcentrifuge tubes Rotor (1.5 ml) Holds (132) 1.5 ml microcentrifuge tubes or (132) 2.
Chapter 6: Accessories for Your CentriVap Installing a Glass Lid Turn off the CentriVap. Unplug the power cord from the wall receptacle. Pull outward on the hinge pin knob. Lift and remove the old lid. To install the new lid, reverse the process. Installing a Secondary Chemical Trap An accessory secondary chemical trap is available to minimize the exhausting of solvents into the atmosphere. Attach the bracket to the left hand side of the housing using the screws provided.
Chapter 6: Accessories for Your CentriVap Be sure to use the proper cartridge for your application. The moisture cartridge is used to trap water vapor. The acid cartridge is used to trap acid vapors. The solvent cartridge is used to trap solvent vapors and the radiochemical cartridge is used to trap radioactive waste. When the media in the insert has changed color, discard the insert and replace it with a new insert.
Chapter 6: Accessories for Your CentriVap Installing a CentriZap™ Strobe Light An accessory strobe light is available to enable you to see the samples as they are rotating in the rotor. Attach the holder to the right hand side of the CentriVap Concentrator using the screws provided. Plug the connector on the strobe light harness into the receptacle on the back of the CentriVap marked “STROBE OUTLET.” Peel the protective backing off the enclosed Velcro® and attach it to the back of the vacuum pump housing.
Chapter 7: Troubleshooting Refer to the following if your CentriVap fails to operate properly. If the suggested corrective actions do not solve your problem, contact Labconco for additional assistance. The following failure codes may appear on the display when problems are sensed by the internal self check routine. CAUTION: Disconnect power before corrective action is taken. DISPLAY ERROR CODE CAUSE CORRECTIVE ACTION Heat Sensor Sensor failure Replace sensor assembly.
Chapter 7: Troubleshooting Other corrective actions for potential problems are as follows: PROBLEM MOTOR ERROR CAUSE CORRECTIVE ACTION Defective motor Replace motor. Hall effect sensor failure Replace sensor. Wire failure Replace wire. Unit not connected to electrical power Connect unit to proper electrical receptacle. Circuit breaker blown Correct electrical problem and reset circuit breaker by pressing button. Lid open Close lid.
Appendix A: CentriVap Components The following pages list components that are available for your CentriVap. The parts shown are the most common replacement parts. If other parts are required, contact Product Service. Item 1 2 2A 3 3A 4 5 6 7 8 9 9A 10 11 12 13 14 15 16 17 18 18A 19 20 20A Not Shown Not Shown Not Shown Not Shown Not Shown 21 22 23 Quantity 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 42.2 inch 2 1 2 1 2 2 1 1 1 1 1 1 1 1 Part No.
Appendix A: CentriVap Components 28 Product Service 1-800-522-7658
Appendix B: CentriVap Dimensions Product Service 1-800-522-7658 29
Appendix C: CentriVap Specifications This Appendix contains technical information about the CentriVap including specifications, environmental operating conditions, wiring diagrams and evaporation rates. Electrical Specifications • Nominal amperage for 115V CentriVap (model 7970010) (including vacuum pump): 5A • Nominal amperage for 230V CentriVap (model 7970011) (including vacuum pump): 3A • Frequency (all models): 50/60 Hz.
Appendix C: CentriVap Specifications Evaporation Rates Solvent Tube Size (ml) 1.5 1.5 1.5 1.5 Number of Samples 132 132 132 132 Sample Size (ml) 1 1 1 1 Temp (C) 45 60 75 24 Time to Dry (min.) 56 41 34 30 Open Open Open Open Rate Overall (ml/min) 2.36 3.22 3.88 5.50 Methanol 1.5 1.5 1.5 1.5 1.5 132 132 132 132 132 1 1 1 1 1 35 45 60 75 100 82 65 50 37 28 Open Open Open Open Open 1.61 2.03 2.64 3.57 4.71 Water 1.5 1.5 1.5 1.
32 Product Service 1-800-522-7658
