KERN & Sohn GmbH Ziegelei 1 D-72336 Balingen E-Mail: info@kern-sohn.com User instructions Polarisation microscope KERN OPE-1 OPE 118 Version 1.0 01/2016 OPE-1-BA-e-1610 Tel: +49-[0]7433- 9933-0 Fax: +49-[0]7433-9933-149 Internet: www.kern-sohn.
GB KERN OBE-1 Version 1.0 01/2016 User instructions Transmitted light microscope Table of contents 1 Before use .................................................................................... 3 1.1 1.2 1.3 1.4 General notes ............................................................................................................................ 3 Notes on the electrical system ................................................................................................ 3 Storage ............
1 Before use 1.1 General notes You must open the packaging carefully, to make sure that none of the accessories in the packaging fall on the floor and get broken. In general, microscopes should always be handled carefully because they are sensitive precision instruments. When using or transporting the microscope it is particularly important to avoid abrupt movements, as this may damage the optical components.
1.3 Storage You should ensure that the device is not exposed to direct sunlight, temperatures which are too high or too low, vibrations, dust or a high level of humidity. The ideal temperature range is between 0 and 40°C and a relative humidity of 85% should not be exceeded. The device should always be located on a rigid, smooth, horizontal surface. When the microscope is not being used, you should cover it with the enclosed dust protective cover.
1.4 Maintenance and cleaning In any event, the device must be kept clean and dusted regularly. If any moisture should be occur, before you wipe down the device you must ensure that the mains power is switched off. When glass components become dirty, the best way to clean them is to wipe them gently with a lint-free cloth. To wipe oil stains or finger prints off the lens surface, moisten the lint free cloth with a mixture of ether and alcohol (70 / 30 ratio) and use this to clean the lens.
2 Nomenclature OPE-1-BA-e-1610 6
3 Technical data / Features Standard configuration Model KERN Optical system Tube Eye pieces Objectives Illumination OPE 118 Finite Monocular WF 10x / Ø 18 mm Achromatic 4x/10x/40x 6V / 20W Halogen (Transmitted) Product dimensions: 360x200x400 mm Packaging dimensions: 440x340x240 mm Net weight: 5,5 kg Gross weight: 6 kg Input voltage: AC 100-240V, 50-60Hz Output voltage: DC 1,2-6V Fuse: 2A 5x20mm 7 OPE-1-BA-e-1610
OPE-1-BA-e-1610 8
4 Assembly 4.1 Polarisation unit + Microscope head Inside the packaging the microscope head is already mounted but inclined towards the rear. At first it must be removed by loosening the fixing screw on the connection point of the tube and then taking it off from the connection point. Instead of the head now the polarisation unit can be attached and fixed by the fixing screw. Thereby the unit can only be aligned in a certain position. This ensures a pin, attached on the bottom side of the unit.
4.4 Colour filter Standardly the microscopes of the OPE-1 series are equipped with a blue colour filter. This filter is firmly connected to a holding ring an you can simply put it onto the housing of the field lens in case of usage. 4.5 Condenser + Polariser The condenser is firmly fixed onto a holding ring (condenser holder) underneath the microscope stage. The lever for the aperture diaphragm is directed towards the front.
5.2 (Pre-) focussing When you are observing an object, you must have the correct distance to the objective to achieve a sharp image. In order to find this distance at the beginning (without other default settings of the microscope) place the objective with the lowest magnification in the beam path, look through the right eyepiece with the right eye and turn it slowly using the coarse adjustment knob (see illustration).
5.3 Centre-adjusting the stage In order to analyse certain objects with help of the polarization method, it is important to be able to revolve the table. Thus, the contrasting of the object can be observed depending on its angle position between polariser and analyser. For getting ideal results the centre of the rotation axis of the table must be aligned to the centre of the optical beam path. The microscopes of the OPE-1 series are correctly set at factory.
1. Bring the 10x objective into beam path. 2. Assure, that one eyepiece with scale is attached to (one of) the tube connector(s). 3. Locate an appropriate specimen slide onto the table. This slide should preferably be equipped with a micro reticule. It would be also possible to use an object which includes plenty of single dots and for which one of those dots has such a size, so that it aligns with the centre cross point of the scale, visible inside of the eyepiece(s). 4.
5.4 Adjusting the magnification After prefocussing has been carried out using the objective with the lowest magnification (see section 5.2), you can then adjust the overall magnification using the nosepiece, as necessary. By turning the nosepiece you can bring any one of the four other objectives into the beam path. When adjusting the nosepiece, you must take the following points into account: - The required objective must be properly locked in place at all times.
5.5 Adjusting the illumination To make sure that perfect image results are achieved during microscopic observation, it is important that the direction of light of the microscope is optimised. The necessary control elements for this are the height-adjustable condenser with aperture diaphragm. When adjusting the lighting for the first time, you must first select the lowest possible objective magnification, so that you can carry out the following steps. 1.
5.6 Adjusting the polarisation unit In order to be able to apply the polarisation method, besides of the bright field method, certain components need to be adjusted. At first you must put the polariser into the beam path. It is attached to the bottom side of the condenser and can be swung in and out. It is important, that the polariser is swung in up to the stop in case of usage. The setting of the analyser now has to be done with help of the according lever, which needs to display 0°.
6 Changing the bulb Halogen Before changing the bulb the device must be switched off and unplugged. To change the bulb, tip the device carefully to the back or side. When doing this, please make sure that all microscope components are firmly fixed. The bulb holder is on the underside of the device. It can be opened by undoing the screws on the holder (see illustration). The defective LED module can be removed by loosening the two screws fixing the module and unraveling the connection point of its cable.
8 Trouble shooting Problem Possible causes The mains plug is not correctly plugged in There is no power at the socket The bulb does not light Defective bulb Defective fuse The bulb blows immediately The field of view is dark The specified bulb or fuse has not been used The aperture diaphragm and/or field diaphragm are not opened wide enough The selector switch for the beam path is set to “Camera” The condenser is not correctly centred You cannot adjust the brightness The brightness control has been se
Problem Possible causes The aperture diaphragm is not opened wide enough The condenser is too low The objective does not belong to this microscope Blurred details The front lens of the objective is dirty Bad image An immersion object has been used without immersion oil Bad contrast The immersion oil contains air bubbles Vignetted field of view The condenser is not correctly centred The recommended immersion oil has not been used Dirt / dust on the objective Dirt /dust on the front lens of the conde
9 Service If, after studying the user manual, you still have questions about commissioning or using the microscope, or if unforeseen problems should arise, please get in touch with your dealer. The device may only be opened by trained service engineers who have been authorised by KERN. 10 Disposal The packaging is made of environmentally-friendly materials, which you can dispose of at your local recycling centre.
