CentrifanTM PE Personal Evaporator User Manual (Shown – Centrifan KD Scientific 84 October Hill Road Holliston, MA 01746 Phone: (508) 429-6809 www.kdscientific.
Notices: This system is covered by a limited lifetime warranty. A copy of the warranty is included with this manual. The operator is not required to perform routine maintenance, but procedures for disassembly and cleaning are as described herein. For cleaning procedures, refer to Chapter 4. All information in this manual is subject to change without notice and does not represent a commitment on the part of KD Scientific.
Warnings and Safety Precautions The following precautions should be followed to minimize the possibility of personal injury TM and/or damage to property while using the Centrifan PE. 1. Maintain a Well-Ventilated Laboratory Volatile organic solvents may be used in the system. Ensure that the laboratory is well ventilated so that a buildup of vaporized solvent cannot occur. 2.
Warnings and Safety Precautions 8. Use the System In A Proper Manner Do not use the instrument and/or its accessories in a manner not specified by KD Scientific. If you do so, the protection provided by safety interlocks may be impaired. 9. Do Not Attempt To Bypass Any Safety Interlocks The safety interlocks are provided to comply with safety requirements of various regulatory agencies and must be employed to protect the operator.
Warranty KD Scientific warranties this instrument for a period of three years from date of purchase. At its option, KD Scientific will repair or replace the unit if it is found to be defective as to workmanship or materials. This warranty does not extend to damage resulting from misuse, neglect or abuse, normal wear and tear, or accident. This warranty extends only to the original consumer purchaser. IN NO EVENT SHALL KD SCIENTIFIC BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES.
Warranty Limitations Products are warranted to be free from defects in materials and workmanship for parts and labor to the original purchaser with the exceptions indicated below: • Any components of the system that are in direct contact with liquids are warranted to be free from defects in materials and workmanship at time of delivery but cannot be further warranted due to the unknown nature of the use of the product.
Safety/Operating Symbols The following symbols appearing in the manual are defined as follows: The WARNING statement used throughout the manual presents dangers that could result in personal injury. The CAUTION statement used throughout the manual presents hazards on conditions that could cause damage to the instrument or the reporting of erroneous results. The NOTE statement used throughout the manual highlights important information about the instrument and its use.
Warranty viii Centrifan TM PE Personal Evaporator
Table of Contents Warnings and Safety Precautions ............................................................................................................iii Warranty ......................................................................................................................................................v Safety/Operating Symbols .......................................................................................................................vii Table of Contents.......................
Table of Contents x Centrifan TM PE Personal Evaporator
Chapter 1 Introduction 1.1 Overview TM The Centrifan PE - Personal Evaporator (Figure 1-1) employs an innovative recirculating evaporation technology to evaporate both aqueous and organic solvents to allow for the isolation of non-volatile solutes from liquid samples. It replaces conventional “blow-down” systems and ensures safe, rapid evaporation of solvent while ensuring sample yield and purity and optimizing solvent recovery.
Chapter 1 1.2 Mode of Operation TM The operation of the Centrifan PE involves spinning of the liquid samples via a rotor and directing a gas flow from a fan over the solvent surface to accelerate evaporation. The vaporized solvent is swept from the sample area and directed to the cold trap where TM the vapors are condensed. The solvent free gas is then returned to the Centrifan PE enclosure to pick up more solvent vapor.
Introduction TM TM Figure 1-3: Evaporation Path in Green; Condensation Path in Black There are two gas flows in the system: a) A primary or evaporation high flow path of recirculating gas removes solvent molecules from the liquid surface of the samples, which are contained in a rotor (right, yellow pathway of Figure 1-3). b) A secondary or condensation low flow loop continuously transfers gas with the solvent vapor to a cold trap for condensation and collection.
Chapter 1 1.3 Benefits of the CentrifanTM PE TM The Centrifan PE system provides a number of benefits compared to commonly employed methods of solvent operation, including: 1.4 • The centrifugal force obtained by rotation of the samples forces the solute to the bottom of the collection vessel. In contrast, when a stream of gas is passed over the sample, it is possible that solute will be distributed into the atmosphere.
Introduction 1.6 • Specifications (Appendix A) - describes general system specifications. • Spare Parts (Appendix B) - lists consumable items and replacement parts for the system. For Further Information For further information, please contact: KD Scientific 84 October Hill Road Holliston, MA 01746 Phone: (508) 429-6809 Fax: (508) 893-0160 www.kdscientific.com or your local KD Scientific Authorized Distributor.
Chapter 1 1-6 Centrifan TM PE Personal Evaporator
Chapter 2 Installation 2.1 Overview Note: The Centrifan user. TM PE Personal Evaporator is designed to be installed by the This chapter describes general information about the location and the physical requirements of the system. 2.2 Location of the System TM The Centrifan PE Personal Evaporator should be installed in a facility with the following environmental conditions: o o o • The temperature range of the laboratory should be between 15 C and 25 C (59 F o 77 F).
Chapter 2 2.4 Electrical Requirements TM The Centrifan PE Personal Evaporator is provided in two versions for either 115VAC or 230VAC. Both versions operate with 50-60Hz and 200 watts. A label on the rear panel indicates the version and the user should ensure that the power requirements match the power provided by the mains. The AC voltage connection to the system must include an earth ground for operator safety.
Installation TM The Centrifan PE Personal Evaporator has been carefully packed and shipped to ensure that it is received in proper condition. Any damage to the container or its contents should be reported immediately to your local distributor or to KD Scientific. Please refer to the warranty (page v), Warranty, Returns, and Repairs, for more information. If replacement parts are needed, please refer to Appendix B, Replacement Parts for part numbers. 2.
Chapter 2 b) Gently withdraw each tab attached to the polypropylene splitter disk from the sample vials. Verify that the knurled retaining nut at the center of the rotor is snugly tightened to retain the rotor within the fan. Remove the vials from the rotor and the rotor is now ready to accept your samples. c) Give the rotor assembly a good spin, tentatively close the lid again and lock the latch.
Installation make the connections from the cold trap. The ½” T-fitting is used to attach a 20mL scintillation vial at the bottom of the cold trap return tubing. This vial helps prevent any condensate which forms in the cold trap exit tube from re-entering the enclosure and slowing the evaporation process. g) If your application will not require drying in an oxygen-free environment, leave the jumper-piece of 1/8” ID tubing between the two small barbed fittings as shown in Figure 2-3.
Chapter 2 2.7 Connections for Evaporation with Inert Gas If you desire to purge the drying enclosure of air by bleeding dry nitrogen or another gas into the process as it runs, then procure a length of 1/8” ID tubing. It should be long TM enough to connect from an inert gas of choice to the back of the Centrifan PE unit and also for providing a vent connection to any convenient exhaust system such as a fume hood or elephant trunk.
Chapter 3 Operation 3.1 Preparing the CentrifanTM for Operation To prepare the system for initial operation: TM Note: The Centrifan PE and the attached cold trap should be placed in a convenient location for loading and removing sample vials and for the occasional addition of dry ice to the cold trap. a) Insure that all hoses are routed correctly and securely pressed onto their fittings. b) Unlatch the lid and open the instrument for rotor access. Remove the splitter disk and any vials.
Chapter 3 3.2 Set-Up the Cold Trap TM (not included in Centrifan -PE- Lite version) 3.2.1 Low-Boiling Solvents a) Fill cold trap with approximately 1L of the desired solvent into the top of the dry ice trap until the level is about 15cm deep. In most cases, denatured ethanol is an appropriate solvent. Note: The solvent that should be used in the cold trap is dependent on the solvent that is being removed from the solvent.
Operation 3.2.2 Frozen Water Bottle Convenience with Higher-Boiling Solvents a) If the solvent to be condensed has a higher boiling point such as Water, IPA, DMF, or DMSO, frozen water is probably sufficiently cold to condense the vapor from the recirculating gas and give a good evaporation rate. Freezing an appropriately sized water bottle is a convenient, no-mess way of condensing most solvents in the trap.
Chapter 3 3.3 Loading Samples in the CentrifanTM PE A view of the splitter separated from the rotor in the Centrifan 3-1.
Operation To load samples in the Centrifan TM PE a) Open the lid and remove the splitter from the openings of the vials sitting in the pockets of the rotor. b) If used vials are present in the rotor, remove them and replace with new vials containing samples that are to be dried. Note: Several different rotors are available for the system.
Chapter 3 h) On a periodic basis, empty the round bottom flask. The waste solvent should be disposed of in a manner that is consistent with local environmental regulations. In some applications, the round bottom flask may contain the desired product. In others, the condensate may be extraction solvent suitable for recycling.
Operation is counting down the minutes, a lighted dot will blink in the top left corner of the display. c) To interrupt the evaporation the timer module can be deactivated by holding down the SET button for 1 second. The unit will beep and display End and OFF showing that the time interval has been deleted. TM Note: To immediately shut off and stop the Centrifan , just open the lid. 3.5.
Chapter 3 3-8 Centrifan TM PE Personal Evaporator
Chapter 4 Maintenance and Troubleshooting 4.1 Maintenance This section describes a series of activities that should be performed on a routine basis to TM optimize the performance and ensure long term, safe operation of the Centrifan PE Personal Evaporator. The suggested frequency for each activity indicated below is based on the “typical operation” of the system and is dependent on a variety of factors such as the nature of the solvent and of the sample.
Chapter 4 Figure 4-1: Remove Nut, Fan, and Two Fittings to Free Liner for Cleaning 4.1.3 Routine Inspection On a periodic basis, the following activities should be performed: a) Inspect the tubing between the evaporator module and the cold trap. If the tubing is cracked, deformed or solid material is deposited inside the tubing, replace the tubing b) Inspect the inert gas supply tubing (or the jumper tube).
Appendix A Specifications Rotor Capacities Temp Controller Range Ambient Temperature Cold Finger Capacity Power Requirements Fuse Ratings Certifications Dimensions Weight Centrifan TM PE Personal Evaporator 6 x 20mL Scintillation Vials 6 x 30mL Scintillation Vials 4 x 40mL Scintillation Vials 8 x 4mL (1 Dram) Vials 8 x 1.
A-2 Centrifan TM PE Personal Evaporator
Appendix B Spare Parts and Accessories Assembly or Part Description CentrifanTM PE-‐T, Includes Trap & Timer (115V) (230V version is 78-‐1070) CentrifanTM PE, Includes Trap (115 V) (230V version is 78-‐1071) CentrifanTM PE-‐L, Lite version, No Trap (115V) (230V version is 78-‐1072) Rotor, CentrifanTM PE, 6 x 20mL Scintillation Vials Rotor, CentrifanTM PE,
B-2 Centrifan TM PE Personal Evaporator
Appendix C Operator’s Note: CentrifanTM PE 8 x 1.6mL Microcentrifuge Rotor Instructions for positioning tube caps when loading the Microcentrifuge tube rotor. The photo below shows the proper orientation of the caps of the Microcentrifuge tubes after they have been loaded into the CentrifanTM PE rotor. Rotate each cap clockwise until it reaches the two o-clock position relative to the tube opening.
Instructions for cutting splitter tabs to accommodate Microcentrifuge tube liquid volumes. Please note that the gasflow splitter for the Microcentrifuge rotor is diecut with long tabs to maximize the drying rate for the last few microliters in the tip of a tube. However, it is not effective to use the long tabs when there is more liquid present because if the tab contacts the liquid then the evaporative gas flow is effectively blocked.
Appendix D Index A Accessories, 1-4, 1 Assembly, 2-3 C Cleaning, 4-1 Cold trap assembly, 2-4 Cold trap, dry ice, 3-2 Cold trap, frozen water bottle, 3-3 Configuration, 1-4 D Drying rates Typical solvents, 3-2 E Electrical Requirements, 2-2 Environmental Conditions, 2-1 Evaporation flow path, 1-3 G Operation, 3-1 S Safety Precautions, iii Safety Symbols, vii Shipping kit, 2-2 Space Requirements, 2-1 Spare Parts, 1 Specifications, 1 Stainless liner Cleaning, 4-1 T Temperatu
Index D-2 Centrifan TM PE Personal Evaporator
