Model 6300 and 6320D Spectrophotometer Operating Manual 630 026 REV G/06-14
Safety Please read this information carefully prior to installing or using this equipment. 1. The 63 series spectrophotometers are designed for operation by trained personnel who are aware of the principles and applications involved. For further help and advice please visit www.jenway.com 2. The spectrophotometer is a sensitive electronic and optical instrument designed for use in a laboratory environment. Careful adherence to the installation instructions must be observed.
respectées. En cas de doute, contactez une personne compétente afin d'obtenir des conseils avant de procéder à l'installation. 3. En plus de suivre les instructions détaillées des manuels d'utilisation et d'entretien de cet instrument, tout le personnel impliqué dans l'installation, le fonctionnement et l'entretien doit connaître et utiliser un système de travail sûr. 4. Des niveaux de tension à risque sont présents dans cet instrument.
. Dieses Gerät ist für minimale Wartungsarbeiten ausgelegt, die unter Einhaltung der in diesem Handbuch dargelegten Verfahren sorgfältig durchzuführen sind. Alle Sicherheitsanweisungen dieser Verfahren sowie die örtlich festgelegten Anweisungen für das Gebiet oder Umfeld, in dem die Arbeit ausgeführt wird, sind einzuhalten. 6. Außer der in den Wartungsverfahren in diesem Handbuch angegebenen Teile gibt es in diesem Instrument keine Teile, die vom Benutzer zu warten sind.
mantenimiento por parte de personal no cualificado dará lugar a la invalidación de la garantía y podría causar gastos adicionales de reparación. 7. Deben consultarse siempre los datos sobre salud y seguridad de los productos químicos o reactivos empleados. Debe leerse atentamente toda la información, los consejos y las advertencias existentes sobre la manipulación, el almacenamiento, el uso y la eliminación de los mismos. Si no se dispone de información, deberá solicitarse al proveedor antes de continuar.
eseguire misurazioni e analisi con questo strumento. Attrezzature idonee per la protezione personale e la sicurezza vanno utilizzate in ogni circostanza. 9. Se si sospetta che la protezione della sicurezza sia stata compromessa in qualsiasi modo, disattivare e rendere inutilizzabile lo spettrofotometro. La condizione di guasto deve essere comunicata al responsabile della manutenzione appropriato. Tale comunicazione deve sempre comprendere il numero di modello e il numero di serie dello spettrofotometro.
Contents SECTION 1 - Introduction .......................................................................................... 9 1.1 1.2 INSTRUMENT DESCRIPTION............................................................................................. 9 INSTRUMENT SPECIFICATION.......................................................................................... 9 SECTION 2 – Installation ......................................................................................... 10 2.1 2.2 2.3 2.4 2.5 2.
SECTION 1 - Introduction 1.1 INSTRUMENT DESCRIPTION The 6300 and 6320D spectrophotometers are suited to a wide range of applications in education, quality control, environmental and clinical analysis. Both models are visible spectrophotometers covering a wavelength range from 320nm to 1000nm, with measurement modes for absorbance, % transmittance and concentration. These models have full interfacing capability for Analogue output and serial (RS232) interfacing.
SECTION 2 – Installation 2.1 UNPACKING Remove the 6300 or 6320D from the packaging and ensure the following items are included: 1. Model 6300 spectrophotometer, or Model 6320D spectrophotometer 2. Required power cable either UK mains lead moulded plug (HH179(S)), EU mains lead moulded plug (HH180(S)), or US mains lead moulded plug (FCABLEUS) 3. Pack of 100 disposable plastic visible wavelength cuvettes (060 084), model 6300 only 4.
The instrument will automatically re-align the monochromator at zero order wavelength. During this initialisation, the instrument displays CAL on the primary display before displaying the main measurement screen. Please note that the instrument will go to the last wavelength and measurement mode used. 2.3 DISPLAY These spectrophotometers have an LCD display: Fig. 2.3.1 – Display 1. Primary display area – Transmission, Absorbance, Concentration 2. Primary display adjust annunciator 3.
Fig. 2.4.1 – Controls 1. Arrow Keys: Used to adjust values on the selected display 2. Arrow Keys: Used to move the cursor horizontally between menu options 3. Return Key: Used to select the display menu option 4. CAL Key: Initiates a calibration routine 5. Print Key. Provides a printout of the current reading with an incremental sample number. When pressed for the first time after a calibration the print out will give calibration information. The incremental sample number will be reset after a calibration.
1. Lamp access panel Allows access to lamp when replacement is necessary. NOTE: The lamp access panel and all ventilation slots must NOT be covered or obstructed at any time. 2.6 2. Power switch On/off switch for the unit 3. Power in socket IEC type connection socket for mains cable 4. Output sockets Analogue output 5. RS232 serial port Connection to a PC SAMPLING Model 6300 is supplied with a 10mm cuvette holder. Other accessories can be fitted (refer to section 7).
SECTION 3 – Theory and Practice of Spectroscopy Measurements 3.1 THEORY OF SPECTROSCOPY MEASUREMENT UV-visible spectroscopy is the measurement of the absorbance of light at a specific wavelength in a sample. This is used to identify the presence and concentration of molecular entities within the sample. The Beer-Lambert law is used to relate the absorption of light to the properties of the sample through which the light is travelling through.
3.2 SPECTROSCOPY MEASUREMENT There are four main components of a spectrophotometer. These are a light source to emit a high and constant amount of energy over the full wavelength range; a method for separating the light into discreet wavelengths; a sample holder and a light detector. The optical layout of the 6300 and 6320D spectrophotometers is shown below: Figure 3.2.
3.3 GOOD PRACTICE GUIDELINES 1. For optimum performance all spectrophotometers should be sited in a clean, dry, dust free atmosphere. When in use ambient temperature and light levels should remain as constant as possible. 2. If required adherence to Standard Operating Procedures (SOP) and Good Laboratory Practice (GLP) should be monitored with regular calibration checks and a suitable Quality Control (QC) programme. 3.
h) Flow-through cuvettes must be selected with care and consideration for the sample type, sample volume, pumping system, rinse, sample and waste handling to be used. 5. Samples and standards should not be stored in open cuvettes or sample containers as evaporation will change the value and lead to staining of the walls which may be irreversible.
SECTION 4 – PHOTOMETRICS MEASUREMENTS The 6300/6320D can perform simple measurements of % transmittance or absorbance. The sample is measured at one wavelength and at one point in time. 4.1 TRANSMISSION MEASUREMENT MODE To select % Transmittance use the LEFT or RIGHT arrow keys to move the cursor to the %T indicator on the menu bar (refer to figure 2.3.1). The primary display will show the transmittance with %T units. To adjust the wavelength use the UP and DOWN arrow keys.
SECTION 5 – CONCENTRATION MEASUREMENTS The concentration measurement mode enables simple measurements of concentration to be performed. In this measurement mode it is possible to calibrate against a standard of a known concentration or use a known factor. The sample is measured at one wavelength at one point in time. To select concentration use the LEFT or RIGHT arrow keys to move the cursor to the CONC indicator on the menu bar (refer to figure 2.3.1).
SECTION 6 – CONNECTING TO A PC Connect the interface cable to the RS232 serial port on the rear of the instrument and connect to the serial port on the rear of the PC. Turn the PC on and load the PC software by inserting the PC software disc into the CD drive. If the PC software does not auto run open My Computer and double click on the 63-Zero software icon. Follow the instructions to install the PC software to the required location.
ASCII SO Opens the dark shutter which allows monochromatic light to enter the sample chamber. This allows 100% transmittance (zero absorbance) to be calibrated. The shutter must be open for normal measurements. ASCII Gnnn Commands the instrument to go to wavelength nnm. For example: G540 will set the wavelength to 540nm. ASCII Fxxxx.x Sets the concentration factor to xxxx.x. For example: F1000 will set the factor to 1000. Note is an ASCII carriage return character.
6300/6320D IBM PC XT (9 way “D”) TDX 2 3 TXD (From PC) RXD 3 2 RXD (To PC) RTS 4 7 RTS (From PC) CTS 5 8 CTS (To PC) DSR 6 6 DSR (To PC) DCD 8 1 DCD (To PC) DTR 20 4 DTR (From PC) GND 7 5 GND Note the interface cable kit (542 009) can be used to implement the above interconnections. 6.2 Recorder Output This is available via the 4mm rear panel sockets. The level is proportional to the displayed reading, depending on the measurement mode: Transmission 1mV per 0.1%T Absorbance 1mV per 0.
SECTION 7 – Accessories and Spare Parts 7.
SECTION 8 – Maintenance and Service 8.1 ROUTINE MAINTENANCE Ensure the external surfaces of the unit are clean and free from dust. The sample area should always be kept clean and any accidental spillage should be wiped away immediately. To give added protection when not in use, the unit should be disconnected from the mains supply and covered with the optional dust cover. The only routine maintenance which maybe required is the replacement of the light source.
For further instructions refer to the service manual. 8.3 SERVICE Our dedicated service staff are on hand to help in the unlikely event that your Jenway equipment develops a fault. Please contact them by one of the following means with a clear description of the problem: E-mail: service@bibby-scientific.com Tel: +44 (0) 1785 810475 Fax: +44 (0) 1785 810471 On occasion it may be necessary for your equipment to be sent back to our Service Department for repair.
SECTION 9 – Troubleshooting 9.1 ERROR CODES Refer to the table below to diagnose error codes. If you require further assistance contact Jenway Help (refer to section 9.2). Error Code Err 1 Error Name Dark cal error Issue This error occurs when the 6300/6320D closes the shutter to block light entering the sample chamber. If the detector output does not fall to a level normally associated with a dark cal then this error is indicated. The most likely cause is that the sample chamber lid is not closed.
SECTION 10 – Declaration of Conformity 27
Bibby Scientific Ltd Bibby Scientific France SAS Bibby Scientific Ltd. – Singapore Beacon Road Stone Staffordshire ST15 0SA United Kingdom Tel: +44 (0)1785 812121 Fax: +44 (0)1785 813748 e-mail: info@bibby-scientific.com www.bibby-scientific.com ZI du Rocher Vert - BP 79 77793 Nemours Cedex France Tel: +33 1 64 45 13 13 Fax: +33 1 64 45 13 00 e-mail: bsf@bibby-scientific.fr www.bibby-scientific.
