Fluorimeters Models 6270, 6280 & 6285 Operating Manual 628 020/REV A/04- 06
Safety This is important information; please read carefully before installing or using this instrument. 1. The 62 Series Fluorimeters are designed for operation by trained personnel that are aware of the principles and applications involved. For further help and advice please contact your local distributor, e-mail sales@jenway.com or visit www.jenway.com 2. The fluorimeter is a sensitive electronic and optical instrument designed for use in a laboratory environment.
Contents: Section 1 Introduction Instrument description Good practice guidelines Section 2 Getting Started Unpacking Installation Section 3 Fluorescence Theory Section 4 Instrument Menu Options Section 5 Instrument Settings Instrument Lock Date, Time and Language Security Operator ID Section 6 Setting up a method Section 7 Using a stored method Section 8 Calibration Section 9 Performing Measurements Section 10 Timed Readings Section 11 Saving Results Section 12 Printing Results Secti
Section 1 Introduction 1.1 Instrument Description Three models are available to cover a wide range of applications. The model 6280 covers the most sensitive determinations with emission wavelengths up to 650nm. Where higher emission wavelengths will be utilised the model 6285 with its redenhanced detector is applicable. For less sensitive applications with a broader wavelength range the model 6270 will meet the necessary requirements.
1.2 Good Practice Guidelines 1. The selection of the optimum excitation and emission wavelengths (filters) is critical to achieving the best performance from the analysis. 2. All fluorimeters should be sited in a clean, dry, dust free environment. When in use ambient temperature and light levels should remain as constant as possible. 3.
10. Check the linear range for each method and, where necessary, use a multi-point calibration or calibration curve. 11. Be aware of the effects of quenching and, where necessary, use sample dilution or extraction methods to eliminate this. 12. Monitor the blank during and between batches to identify any increase due to contamination. 13.
Section 2 Getting Started 2.1 Unpacking Remove the universal 12V power supply (with UK, US and EU leads) and the pack of 100 disposable cuvettes from the packaging. Remove the fluorimeter from the carton by lifting it in the centre between the two support cheeks; do not lift it by the support cheeks. Place all items on a clean workbench then remove the support cheeks and the polythene bag from the fluorimeter.
2.24 Keypad Functions 3 1 4 2 7 5 6 1. UP ARROW key 2. DOWN ARROW key 3. LEFT ARROW key 4. RIGHT ARROW key 5. ENTER key 6. CAL key 7. PRINT key - 2.25 used to navigate through menus, to increase values and for paging up in stored results. used to navigate through menus, to decrease values and for paging down in stored results. used to navigate through menus and to highlight the selected digit when setting values. used to navigate through menus and to highlight the selected digit when setting values.
2.26 Rear Panel Layout 1. Output Socket – 9 way output socket for RS232 2. Switch – Power On/Off switch 3. Connection Sockets – 4 x pin sockets for heated cell block controller. 4. Power In Socket – Connection socket for 12V DC universal mains adapter 2.
2.28 Power on and Self-Tests Connect the mains supply cable to the rear panel mains input socket and plug the other end into a suitable mains supply socket. Lift the sample chamber lid on the instrument and ensure that there is no sample or other item present in the sample holder, close the lid. Switch on the supply socket, then the instrument, using the Power switch located on the rear panel. The instrument will then perform a power on self-test protocol. The following screen will be shown for approx.
Example Menu Operation A common operating system is used throughout this and similar Jenway products, a brief overview of navigating through the menu system with the cursor keys follows; Use the up and down arrow keys to highlight a menu option, press the enter key to confirm.
Section 3 Fluorescence Theory The interaction between electromagnetic radiation and matter provides a useful, qualitative and quantitative analytical tool, known as spectroscopy. The region of the electromagnetic spectrum, to which matter under investigation is subjected to, defines the type of transitions that occur within the molecules. Fluorimetry uses radiation from the UV-Vis region of the electromagnetic spectrum to study transitions between electronic levels in a molecule or atom.
Section 4 Instrument Menu Options In this section a brief explanation is given for the main menu options available. These will be covered in greater depth in the sections appropriate to their usage. Measure Screen METHOD NAME - user set current method name or DEFAULT METHOD LAST READING - result from last read or --.--UNITS OF MEASURE - as set in MEASURE SETUP menu SYSTEM GAIN% - 0 to100% as set up in MEASURE SETUP menu. Note: This option is not available on the Model 6270.
NAME – user entered method name (up to a maximum of 19 characters) EXCITATION NM – user set excitation wavelengths. Actual filter data is checked against this setting during measurement. If an incorrect filter is detected a warning will be displayed. EMISSION NM – user set emission wavelengths. Actual filter data is checked against this setting during measurement. If an incorrect filter is detected a warning will be displayed.
FLUORESCENCE TABLE – shows results of last calibration RFU (raw fluorescence) values. Tabular and graphical displays available P.M.T GAIN(%) - 0 to 100% gain setting for system photomultiplier tube. Note: This option is not available on the Model 6270. AUTO SET GAIN - uses the highest user entered calibration standard to automatically set the PMT gain to give as close as possible to 95% full scale emission signal. Note: This option is not available on the Model 6270.
Instrument Setup Menu From the MAIN MENU press the down arrow key to move the highlight on the display to cover INSTRUMENT SETUP, then press the enter key. The following display will be shown: EXIT - allows the user to return to the previous menu LOCKED - when enabled, prevents access to set up menus without the PIN code (settings of current method to be viewed but not changed). Calibrations can still be performed when the instrument is locked.
Section 5 Instrument Settings Note: Using the left arrow key to move from the settings on the right hand side of the menu will abort any adjustments you have made and return the value or selection to its previous settings. The enter key must be used to confirm any changes. All settings listed below are accessed from the INSTRUMENT SETUP menu as shown below: The 62 Series Fluorimeters offer a number of important functions to control or restrict use of the instrument.
5. To set or change the locking code, press the down arrow key to select LOCKING CODE, then press the enter key. 6. Adjust the highlighted digit using the up or down arrow keys, then select the other digits using the right or left arrow keys. Adjust each in turn until the code of your choice is displayed. Press the enter key to confirm this, ensuring that you can remember the number selected. 7.
3. When the language of your choice is highlighted press the enter key to confirm. The language used on the display will now be changed to match your choice. 4. To exit the INSTRUMENT SETUP menu use the up or down arrow keys to highlight EXIT, then press the enter key. To set additional parameters continue as detailed in the following text. Time 1. Press the down arrow key to move the highlight to TIME and press the enter key. 2.
4. Use the down arrow key to select DATE FORMAT. Press the up or down arrow keys to view the two alternative date formats. Press the enter key to confirm your choice. The previously set date will automatically change with your selection in this menu option. 5. To exit the INSTRUMENT SETUP use the up or down arrow keys to highlight EXIT, then press the enter key. To set additional parameters continue as detailed in the following text.
Section 6 Setting up a method 1. Enter MEASURE SETUP menu by selecting SETUP from the MAIN menu. 2. Select a suitable METHOD ID (0-19). 3. Give the method a NAME (up to 19 characters) using the alphanumeric screen accessed by selecting the NAME option of the MEASURE SETUP menu. 4. Return to the MEASURE SETUP menu and set the EXCITATION wavelength, in nm for the test you wish to perform. The excitation filter module fitted for this test will need to be the same wavelength. 5.
8. The number of decimal places (RESOLUTION) to which the measurements will be displayed can then be set at the next option in the MEASURE SETUP menu. The fluorimeter will auto range resolution for measurements too large to be displayed in the set resolution. 9. To define the calibration parameters, select the last option of the MEASURE SETUP menu, CALIBRATION SETUP. 10. Once in the CALIBRATION SETUP menu, set the NUMBER OF STANDARDS that will be used at calibration.
13. Set the photomultiplier tube gain (P.M.T. GAIN %) to the required percentage, for this test, if known. If not known, use the AUTO SET GAIN option as below. Note: This option is not available on the Model 6270. 14. To find the optimum gain for a given test, select the AUTO SET GAIN menu option and follow the instructions on the instrument screen. If you accept the gain suggested by the instrument, this will then automatically update the gain setting in the CALIBRATION SETUP menu.
16. The method is now fully set and will be the available in the MEASURE screen. Section 7 Using a stored method 1. From the MAIN menu, select the SETUP menu and then MEASURE SETUP. 2. By setting the METHOD ID to the appropriate number (0-19) the saved parameters such as the method name and wavelengths will appear and be ready for use. 3. EXIT the setup menus and return to the MAIN menu. 4. Ensure that the excitation and emission filters, appropriate to selected method, are fitted in the sample chamber. 5.
Section 8 Calibration 1. Before beginning the calibration, ensure that close to hand, are the appropriate cuvette/s, blank solution and standard solutions (as set in the CALIBRATION SETUP of the MEASURE SETUP menu). 2. Using the steps as detailed in the 'Using a stored method' section, select the required method. 3. Once in measure mode, press the CAL key once to initiate the calibration sequence. 4. The instrument display will show the message "Please insert blank…" 5.
9. The options at the bottom of the screen will have also changed. The ACCEPT and REPEAT options relate to the calibration step just completed. If the concentration and other data from the calibration are correct and satisfactory, select ACCEPT to continue through the calibration sequence to the first standard. If the previous step has given unacceptable figures, then select REPEAT and perform the previous step again.
Section 9 Performing measurements 1. Using the steps as detailed in the 'Using a stored method' to select the required method. 2. Prior to taking any measurements make certain that the correct excitation or emission filter modules are fitted and that a valid calibration (as detailed in Section 8) has been completed. 3. Before taking any measurements it is worth considering the utilisation of functions such as AUTO SAVE and AUTO PRINT to provide easy access and analysis of results at a later time.
Section 10 Timed readings 1. To ensure that all measurements are taken at the required interval, select the required method as described in section 'Using a stored method' above, then return to the main menu. 2. Enter the SETUP menu and select the RESULTS STORAGE section. 3. Moving down to the first parameter, TIMED READ, set this to On. 4. The interval between the end of one read and the beginning of the next read can then be set, by selection of INTERVAL.
Section 11 Saving results Saving results 1. When a reading has been taken in the MEASURE mode, the concentration result is displayed on screen and can be saved to the instrument internal memory by selecting the SAVE option on the right hand side of the MEASURE screen. To confirm the save is successful the message "Result Saved…" is shown briefly on screen. 2. A maximum of 100 results can be saved in this way, for each method.
Stored results/Results mode 1. To access the saved results for the method currently selected in MEASURE SETUP, return to the MAIN menu. Using the down arrow key, move the highlight to RESULTS and press the enter key. 2. A table of all saved results, for the method named at the top of the screen will be shown along with the time at which the READ operation occurred. The results are presented in chronological order, beginning with the most recent. 3.
7. Further analysis of the saved results can be made by accessing the STATISTICS screen. To move to STATISTICS, use the option at the bottom of the GRAPH screen. 8. The STATISTICS screen shows two numbers calculated from the saved results. The SLOPE reveals the extent of change of fluorescence over time. The R-squared value gives an indication of the linearity of the saved results. Section 12 Printing results Printing 1.
DataWay 1. Connect the supplied cable between the PC and the instrument RS232 socket. 2. If using for the first time, insert the DataWay CD into the CD drive of the PC. The CD will automatically run and installation instructions will be shown on the PC screen. 3. Once installed the software can be opened either from the programs menu or with the DataWay icon on the PC desktop. 4. Open the DataWay software. A pop-up box will appear as the software starts up.
Section 13 Maintenance & Troubleshooting 8.1 General The 62 Series Fluorimeters are designed to give optimum performance with minimum maintenance. It is only necessary to keep the external surfaces clean and free from dust. The sample area should always be kept clean and any accidental spillage should be wiped away immediately. To give added protection when not in use, the unit should be disconnected from the mains supply and covered with the optional dust cover (630 028).
Section 14 Optional Accessories 14.
Interference Filters (continued) 627 167 Wavelength 460nm 627 136 Wavelength 470nm 627 168 Wavelength 480nm 627 169 Wavelength 490nm 627 170 Wavelength 500nm 627 171 Wavelength 510nm 627 137 Wavelength 515nm 627 172 Wavelength 520nm 627 173 Wavelength 530nm 627 174 Wavelength 532nm 627 175 Wavelength 540nm 627 176 Wavelength 546nm 627 177 Wavelength 550nm 627 178 Wavelength 560nm 627 179 Wavelength 570nm 627 180 Wavelength 577nm 627 181 Wavelength 580nm 627 182 Wavelength 590nm 627 183 Wavelength 600nm 627
Section 15 Specification & Data 10.
Glossary of Terms Filters - There are three types of filter commonly used in fluorimeters, Band-pass filters, Cut-Off filters and Interference filters. The first two offer a low-cost general purpose option while the more expensive interference filters offer precise selection of excitation or emission wavelengths. Often one type will be used for excitation and another for emission depending on the proximity of interfering wavelengths.
EC Declaration of Conformity Jenway Models 6270, 6280 and 6285 Fluorimeters comply with the following European Standards: EN 50081–1: 1992 Electromagnetic compatibility – Generic emission standard EN 50082-1: 1992 Electromagnetic compatibility – Generic immunity standard EN 61010-1: 2001 Safety requirements for electrical equipment for measurement, control and laboratory use Following the provision of: EMC Directive – 89/336/EEC and Low Voltage Directive – 73/23/EEC Carl Warren General Manager, Jenway, Gr
