Operating Manual Instruction Manual

8 - 1
PN 074-519-P1C
3000 Micro GC Operating Manual
Chapter 8
Instrument Operations
8.1 Routine Operation
8.1.1 System Bakeout
Over time, small amounts of contaminants may accumulate in 3000 Micro GC.
Contaminants in the chromatography column may cause peak tailing and retention
time shifts. To clear the contaminants, create a bakeout method and run it:
 After the first installation
 After installing a replacement GC module
 After the 3000 Micro GC has been turned off or stored for an extended period
of time
 After the 3000 Micro GC has been stored in a Shipping Case
(PN G2801-81005) for an extended period of time
 Periodically as needed to refresh column performance
Proceed as follows to create and run a bakeout method.
1 In EZ IQ, click Method >> Instrument Setup.
2 Enter the following acquisition parameters.
 Turn on Detector Filaments
 Set Column Temperature as suggested in Table 8-1
An example bakeout method is shown in Figure 8-1.
Table 8-1 Bakeout temperatures for various columns
Column type Column
temperature, °C
Duration,
hours
Recommended frequency
for general use
Alumina PLOT 180 8–12 Weekly
MolSieve 5A PLOT 180 8–12 Weekly
OV-1 180 2 Weekly
OV-1701 180 2 Weekly
PLOT Q 160 8–12 Weekly
PLOT U 160 8–12 Weekly
Stabilwax-DB 180 2 Weekly