Micro10x User Manual Rev 2.
Index Introduction ............................................................................................................... 3 Section 1 - Setup ........................................................................................................ 4 1.1 - Tubing ..................................................................................................................... 4 1.2 - Plate Nest ........................................................................................................
Introduction The Hudson Robotic Micro10x™ is a fast, compact, accurate benchtop instrument for reliable, automated bulk reagent dispensing into all microplates. The Micro10x provides a combination of fast filling speeds, high accuracy and extreme flexibility not found in other benchtop reagent dispensers. The Micro10x accurately dispenses volumes down to 1uL per channel, and provides features like tip-touch to ensure a clean, full dispense of even high surface-tension liquids.
Section 1 - Setup 1.1 - Tubing The MC10X is shipped with two tube assemblies (Figure 1.1.1). The tube assembly with the two Flangeless fittings should be installed between the right port on the pump head and the MC10X manifold. To create a proper seal tighten the fittings into the pump head and manifold until they stop finger tight. Once the fitting is finger tight turn the fitting an additional 1/8 turn to ensure a proper seal. (Figure 1.1.
Right pump port Manifold port Figure 1.1.2 Left pump port Figure 1.1.
The priming trough uses a short silicon drain tube and waste basin. The silicon tube can be replaced with a longer one if needed. Standard silicon tubing will work as a replacement (Figure 1.1.4). Priming Trough Figure 1.1.4 1.2 - Plate Nest Install the plate nest onto the nest holder. The legs of the plate nest should fit snugly into the mounting position. The plate nest can be positioned in either the portrait or landscape orientation. In Figure 1.2.
1.3 - Manifold Install the manifold with the inlet port closest to the pump head. Use the provided thumbscrews to secure the manifold onto the mounting flange (Figure 1.3.6). Thumbscrews Figure 1.3.6 1.4 - External Cables The Micro10X Comes with a few cables that need to be installed into the rear of the unit before the unit can be turned on. The first cable is the pump cable, this is a black coiled cable that has two 90 connectors.
Port for optional 4-way valve Pump Cable Controller Cable Pump Cable Serial Port DC Inlet for Power cord Figure 1.4.
Section 2 - Operation of Micro10X through the Touch Screen Controller 2.1 - Controller Features The Micro10X comes with a windows based touch screen to control the unit. On the top cover of the Micro10X there are two locations to slide the controller into (Figure 2.1.1). So it doesn’t matter which way the unit is facing, because you will able to access the control panel from either orientation (Figure 2.1.2).
2.2 - Main Menu When you power the MC10X up, it will automatically boot and start the MC10X program on the controller. The unit will also home all the axes when the unit is powered up. After initializing successfully the controller will display the main menu screen. From there you can access the following sub menus: Dispense, Prime, Empty Dispenser, Auto-Prime, Change Settings (Figure 2.2.1). Figure 2.2.1 2.3 - Dispense Submenu The MC10X can support up to 20 dispense programs.
Figure 2.3.1 Volume – Any integer value from 1 to 9999uL may be selected. Speed – This adjusts the percentage of the pumps maximum speed. More viscous liquids will require a slower pump speed to avoid cavitation. Depth – Negative values are the distance below the travel height where the tips will move during the dispensing, allowing the user to dispense with the tips in the wells. Positive values will move the dispensing location above the travel height.
Orientation– Select between the Portrait or Landscape plate orientation. This is determined by which manifold you are working with.12 channel will run in landscape while the 8 channel runs in portrait configuration. Tip Touch – Activate or deactivate tip touching. Tip Touch – Adjust where in the plate well you would like the tips to touch after the dispensing. Depending on the plate type, you will center your tips in row A of either a 96 or 384 well plate.
Figure 2. 4.1 Volume – This is the total volume that will be primed through the system. Enter a value between 120 and 9999uL. Speed – This adjusts the percentage of the pumps maximum speed. More viscous liquids will require a slower pump speed to avoid cavitation. Do not exceed more than 30% speed on any value over 1000ul. Tip Touch – Activate or deactivate tip touching after each dispense. Tip Touch – Adjust where in the Priming trough you would like to touch the tips after a prime.
Figure 2.5 1 2.6 - Auto-Prime Priming can be programmed to occur at a user specified interval. This allows the MC10X to dispense a certain amount of fluid into the priming trough to prevent evaporation during every specified time interval. Setting the time to 0 turns off this feature (Fig. 2.6.1).
Figure 2.6.1 Time – This is the interval in seconds; a time of 0 deactivates this feature Volume – This is the total volume that will be moved through the manifold. Enter a value between 120 and 9999uL. Speed – This adjusts the percentage of the pumps maximum speed. More viscous liquids will require a slower pump speed to avoid cavitation. Do not exceed more than 30% speed on any value over 1000ul. 2.7 - Settings Submenu There are critical settings that can be adjusted by an administrator (Figure. 2.7.1).
Figure 2.7.1 Figure 2.7.
Adjust Prime Position – This allows the user to jog the dispensing manifold to the position where they would like all priming functions to occur. When you click on the button you will be asked if you want to move to the location. If you are unaware of where the location is, click no and work from the home position (Figure 2.7.3). After making an adjustment in any of the fields press “OK”. To lose the changes press “Cancel” (Figure. 2.7.4).
Figure 2.7.4 Figure 2.7.5 Adjust Track/Nest Height – This value should be evaluated and adjusted if manifold types or plate nests are changed. This will set the 0 position for the Zaxis. All dispense programs will reference this point to determine absolute dispense height.
plate nest without touching it. To adjust, place a sheet of paper or business card on the plate nest. Jog the manifold down until it touches the paper but not pinch it to the nest. (Figure 2.7.6) Figure 2.7.6 Adjust Plate Origin – This value should be evaluated and adjusted if manifold types or plate nests are changed. This is the reference point for all well positions in a SBS standard plate.
Figure 2.7.7 Adjust Row/Column Spacing - This setting is set by the manufacturer and should not be adjusted. # Of Tips in Manifold - This setting is set by the manufacturer and should not be adjusted. 2.8 - Starting and Stopping a Program Press the Next or Prev key on the TouchScreen to select the program number you would like to run. When the desired program is selected press the RUN button on the TouchScreen (Figure 2.8.1). To stop a program, press the CANCEL button on the TouchScreen.
Figure 2.8.
Section 3 - Maintenance of the Mirco10X Fluid Path Note: Please turn power off and disconnect all cables from the rear of the Micro10x before performing any maintenance on the unit. 3.1 - Removal of the Pump Head Remove the 4 mounting screws from the face of the pump (Figure 3.1.1). Support the pump with your hand to prevent it from falling from the mounting plate. 4 mounting screws for Pump head Figure 3.1 1 Gently pull the pump diagonally away from the mounting plate.
Figure 3.1 2 Pivot Bearing Figure 3.1.
3.2 - Disassembly of the Pump Head Module After the pump head module is removed from the pump you will able to access the Teflon adapters. The adapters lock the liner and piston into the proper position inside the cylinder case. You will remove the adapters by using a 5/8” open ended wrench and turning the adapters counter clockwise until they are removed from the cylinder case (Figure 3.2.1).
Figure 3.2 3 Figure 3.2.4 With the Seal nut loose you will carefully slide the piston, seals and seal nut out of the cylinder case (Figure 3.2 5). Make sure that the seals do not come off the piston if you force the seals back on you may damage the surface of the seal that sits against the piston. If this happens you will have to replace the seal with a new one.
Figure 3.2.5 3.3 – Cleaning of the Pump Assembly All of the pump parts may be cleaned in an ultrasonic cleaner with a dilute detergent solution. The parts must be thoroughly rinsed prior to autoclaving. 3.
Item # 1 2 3 4 5 6 7 QTY 1 1 2 1 1 2 1 Part Number PMP-1018 PMP-1021 PMP-1019 PMP-1017 PMP-1020 MCF1-18202-00 MCF1-18201-00 Description Cylinder Nut Gland Washer, ¼”. Teflon Lip Seal, ¼”, Rulon RA Q1C Cylinder Liner/Piston Set Cylinder Head Seal Adapter, Teflon Cylinder Case 3.
Section 4 – Micro10x Validation Section 4.1 – General Validation After Cleaning To Ensure that the unit is dispensing properly after cleaning you will need to perform a validation dispense. To perform a validation dispense you will need the following items: Clear 96-well Polypropylene Plate Bulk reagent to dispense (preferably not clear) Optional- Sartorius WZA225-CW balance or similar with at least a 0.001mg readability.
Section 5 - Hardware Specifications Section 5.1 - General NOTE: Specifications are subject to change without notice. Micro10X: Plate Format: Housing Material: Plate Nest Material Number of Axes 96, 384, or 1536 well plates in any shallow, Medium or Deep well-formatted SBS plate. Painted steel covering anodized cast aluminum housing Peek Three (X, Y and Z axes) Section 5.2 - Mechanical Description X-axis: Integrated stepper motor/stepper controller with encoder.
Liner Bore Size: Tubing Material: Max Shot Size Min Shot Size Max Flow ¼” Peek fittings on Telfon tubing 225ul per revolution 12ul per revolution 0.225 lps (60% Max speed) Section 5.3 - Manifold Fixed Tip Manifold Material: Fixed tip Material: Peek Stainless Steel Section 5.4 - Dimensions 11½”H X 7”W X 10”D (Does not include Nest plate) 11½”H X 7”W X 15”D (Includes Nest Plate) Weight: ~15LBS Section 5.
