Specifications

47
Output settings for calculations
• Vector y=ax+b - curve equation
in the case of linearity
• Square sum of the residuals
(only calibration function)
• Residual standard deviation
• Mean value and standard
deviation of y
• Multiple correlation coefficient
• Results of F- and t-tests
• Method standard deviation
Limit of detection and quantifi-
cation
The detection limit is the smallest
amount of substance that can be
detected qualitatively during one
analysis with a defined statistical
certainty. The quantification limit
is the smallest amount of a sub-
stance that can be detected quan-
titatively during one analysis with
a statistical certainty to be deter-
mined. The quantification limit is
higher than the detection limit.
You need the relative error of
results to calculate the determina-
tion limit. Both the detection limit
and the quantification limit can be
calculated by the standard devia-
tion of blank values (blank value
method) or the residual standard
deviation of regression data (cali-
bration curve method). Only one
of the two methods may be suit-
able for practical purposes. Both
methods, however, are almost
equal with respect to the detection
limit. The calibration curve
method is suited to determine the
quantification limit. Figure 32
shows the planning dialog of
checkpoint “limit of detection/
quantification”
Figure 32
Planning dialog of checkpoint “Limit of Detection/qQantification”
Planning data
• Determination method:
• Blank value method
according ICH und signal to
noise ratio)
• Calibration line method
according to ICH
(no calculations executed,
only display of chromato-
gram!)
• Signal/noise ratio
• Other data
• Number of samples
• Y-units
• Multiple injection possible
Output settings for calculations
• Standard deviation
• Procedure standard deviation
• Critical value yk
• Residual standard deviation
(additional for calibration curve
method)