Instruction Manual HI 96801 Refractometer for Sucrose Measurements w w w. h a n n a i n s t .
Dear Customer, Thank you for choosing a Hanna Instruments product. Please read this instruction manual carefully before using this instrument. This manual will provide you with the necessary information for correct use of this instrument, as well as a precise idea of its versatility. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com or view our worldwide contact list at www.hannainst.com. TABLE OF CONTENTS PRELIMINARY EXAMINATION ..............................
GENERAL DESCRIPTION The HI 96801 Digital Sucrose Refractometer is a rugged portable, water resistant device that benefits from Hanna’s years of experience as a manufacturer of analytical instruments. The HI 96801 is an optical instrument that employs the measurement of refractive index to determine the % Brix of sugar in aqueous solutions. The method is both simple and quick. Samples are measured after a simple user calibration with deionized or distilled water.
PRINCIPLE OF OPERATION The Brix determination is made by measuring the refractive index of a solution. Refractive Index is an optical characteristic of a substance and the number of dissolved particles in it. Refractive Index is defined as the ratio of the speed of light in empty space to the speed of light in the substance. A result of this property is that light will “bend”, or change direction, when it travels through a substance of different refractive index. This is called refraction.
FUNCTIONAL DESCRIPTION TOP VIEW 1. Liquid Crystal Display (LCD) 2. Secondary Display 3. Primary Display 4. READ Key (User Measurement) 5. ZERO Key (User Calibration) 6. ON/OFF 7. Stainless Steel Sample Well and Prism BOTTOM 8. Battery Cover 9.
DISPLAY ELEMENTS 1. 2. 3. 4. 5. 6. 7. Battery (blinks when low battery condition detected) Primary Display (displays measurement and error messages) Measurement in Progress Tag SETUP: Factory Calibration Tag CAL: Calibration Tag Measurement Unit Automatic Temperature Compensation (blinks when temperature exceeds 10-40 °C / 50-104 °F range) 8. Temperature Units 9.
CALIBRATION PROCEDURE Calibration should be performed daily, before measurements are made, when the battery has been replaced, between a long series of measurements, or if environmental changes have occurred since the last calibration. 1. Press the ON/OFF key, then release. Two instrument test screens will be displayed briefly; all LCD segments followed by the percentage of remaining battery life. When LCD displays dashes, the instrument is ready. 2.
MEASUREMENT PROCEDURE Verify the instrument has been calibrated before taking measurements. 1. Wipe off prism surface located at the bottom of the sample well. Make sure the prism and sample well are completely dry. 2. Using a plastic pipette, drip sample onto the prism surface. Fill the well completely. Note: If the temperature of the sample differs significantly from the temperature of the instrument, wait approximately 1 minute to allow thermal equilibration. 3. Press the READ key.
MAKING A STANDARD % BRIX SOLUTION To make a Brix Solution, follow the procedure below: • Place container (such as a glass vial or dropper bottle that has a cover) on an analytical balance. • Tare the balance. • To make an X BRIX solution weigh out X grams of high purity Sucrose (CAS #: 57-50-1) directly into the container. • Add distilled or deionized water to the container so the total weight of the solution is 100g.
BATTERY REPLACEMENT To replace the instrument’s battery, follow these steps: • Make sure the instrument is off. • Turn instrument upside down and remove the battery cover by turning it counterclockwise. • Extract the battery from its location. • Replace with new 9V battery making certain to observe polarity. • Insert the back battery cover and fasten it by turning clockwise to engage.
ERROR MESSAGES Error Code Descript ion “Err“ General failure. Cycle power to instrument. If error persists, contact Hanna. “LO“ primary display Sample exceeds minimum measurement range. “HI“ primary display Sample exceeds maximum measurement range. “LO“ primary display Cal segment ON Wrong solution used to zero instrument. Use deionized or distilled water. Press ZERO. “HI“ primary display Cal segment ON Wrong solution used to zero instrument. Use deionized or distilled water. Press ZERO.
Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA Technical Support for Customers Tel. (800) 426 6287 Fax (401) 765 7575 E-mail tech@hannainst.com www.hannainst.