Instruction Manual HI 96765 Blood Plasma Analyzer www.hannainst.
Dear Customer, Thank you for choosing a Hanna product. This manual will provide you with the necessary information for the correct use of the instrument. Please read it carefully before using the meter. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com. TABLE OF CONTENTS PRELIMINARY EXAMINATION .............................................................................................. 3 GENERAL DESCRIPTION ...................................................
PRELIMINARY EXAMINATION Please examine this product carefully. Make sure that the instrument is not damaged. If any damage occured during shipment, please notify your Dealer. Each HI 96765 Blood Plasma Analyzer is supplied complete with: • Light Blocking Cap • 9V Battery • Cloth for wiping tubes • Instrument quality certificate • Instruction Manual • Rigid carrying case Note:Save all packing material until you are sure that the instrument works correctly.
GENERAL DESCRIPTION The HI 96765 meter measures the light absorbance of animal blood plasma: the absorbance value allows identification of the plasma quality. Display codes aid the user in routine operations. The meters have an auto-shut off feature that will turn the instrument off after 10 minutes of non-use. This instrument has been developed to measure centrifuged animal blood, coming from abattoirs. The quality of the blood is checked by measuring its color, expressed in Quality Units.
PRINCIPLE OF OPERATION Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules or crystal lattices.
A microprocessor controlled special tungsten lamp emits radiation which is first optically conditioned and beamed to the sample contained in the cuvette. The optical path is fixed by the diameter of the cuvette. Then the light is spectrally filtered to a narrow spectral bandwidth, to obtain a light beam of intensity Io or I. The photoelectric cell collects the radiation I that is not absorbed by the sample and converts it into an electric current, producing a potential in the mV range.
KEYPAD DESCRIPTION • ON/OFF: to turn the meter on and off. • CAL: to calibrate the meter prior to measurement. • READ: to make a measurement. OPERATING MODES • Measurement mode: default operation mode, enables both calibration and measurement.
ERRORS AND WARNINGS The instrument shows clear messages when erroneous condition appears. Messages are also displayed when the obtained values are outside expected range. The beeper is playing a beep on errors. a) on calibration Light High: There is too much light to perform a measurement. Light Low: There is not enough light to perform a measurement. No Light: The instrument cannot adjust the light level. Please check that the tube holder does not contain any debris.
Over Range: A flashing value of the maximum concentration indicates an over range condition. c) other errors and warnings Cap error: An excess ambient light is reaching the detector. Please place the light blocking cap and press CAL again. If the light blocking cap is properly installed, and a calibration can not be performed, then contact your dealer or the nearest Hanna Customer Service Center. Cooling lamp: The instrument waits for the lamp to cool down. Battery low: The battery must be replaced soon.
GENERAL TIPS FOR AN ACCURATE MEASUREMENT The instructions listed below should be carefully followed during testing to ensure best accuracy. • Color or suspended matter in large amounts may cause interference, therefore these should be removed by treatement with active carbon and by prior filtration. • For a correct filling of the tube: the liquid in the tube forms a concavity on the top; the bottom of this concavity must be at the same level of the 1.5 mL mark.
MEASUREMENT PROCEDURE To compensate the meter for the sample turbidity or color, the measurement takes place in two phases. First, the meter is calibrated. After the sample is prepared, the sample is measured. • Turn the meter on by pressing ON/OFF. The display briefly shows all tags on. • When the beeper sounds briefly and the LCD displays dashes, the meter is ready. The blinking “CAL” indicates that the instrument needs to be calibrated first. • Fill a standard 10 mm tube with 1.5 ml of the blood sample.
• Press CAL and the lamp, tube and detector icons will appear on the display, depending on the measurement phase. CAL • Wait for a few seconds and the display will show “-0.0-”. The meter is now calibrated and ready for measurement. NOTE: If the “CAP” message appears, then there is too much ambient light. Please place the light blocking cap and press CAL again.
• Insert the centrifuge tube into the holder. • Press READ and the lamp, tube and detector icons will appear on the display, depending on the measurement phase. READ • The instrument directly displays the Blood Plasma Quality Units on the Liquid Crystal Display. INTERFERENCES Suspended blood cells and solids: repeat pretreatment. Strong ambient light: use the light blocking cap for calibrating the instrument, and while taking readings.
The instrument displays a battery indicator with three levels as follows: • 3 lines for 100 % capacity • 2 lines for 66 % capacity • 1 line for 33 % capacity • Battery icon blinking if the capacity is under 10 %. If the battery is empty and accurate measurements can’t be taken anymore, the instrument dead batt shows “dead batt” and turns off. To restart the instrument, the battery must be replaced with a fresh one.
WARRANTY HI 96765 is warranted for two years against defects in workmanship and materials when used for its intended purpose and maintained according to the instructions. This warranty is limited to repair or replacement free of charge. Damages due to accident, misuse, tampering or lack of prescribed maintenance are not covered. If service is required, contact your dealer. If under warranty, report the model number, date of purchase, serial number and the nature of the failure.
Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA Technical Support for Customers Tel. (800) 426 6287 Fax (401) 765 7575 E-mail tech@hannainst.com www.hannainst.
