Instruction Manual HI 964400 Microprocessor Bench Dissolved Oxygen Meter This Instrument is in Compliance with the CE Directives www.hannainst.
Dear Customer, Thank you for choosing a Hanna Instruments Product. Please read this instruction manual carefully before using the instrument. This manual will provide you with all the necessary information for the correct use of the instrument, as well as a precise idea of its versatility in a wide range of applications. This instrument is in compliance with directives EN 50081-1 and EN 50082-1. TABLE OF CONTENTS Preliminary Examination ............................. 1 General Description ..................
PRELIMINARY EXAMINATION Remove the instrument from the packing material and examine it to make sure that no damage has occurred during shipping. If there is any damage, notify your Dealer. Each bench D.O. meter HI 964400 comes supplied complete with: • HI 76407/2 D.O. probe with 2 m (6.7') cable • HI 76407A membrane cap (2 pcs) • HI 7041S electrolyte solution (30 ml) • 12 VDC power adapter (HI 710005 or HI 710006) • dust cover.
The dissolved oxygen probe has a membrane covering the polarographic sensors and a builtin thermistor for temperature measurements and compensation. This permeable PTFE membrane isolates the sensor elements from the testing solution, but allows oxygen to pass through. When a voltage is applied across the sensor, oxygen that has passed through the membrane reacts causing a current flow, and hence determining a reading.
FUNCTIONAL DESCRIPTION PROBE 3 1 4 4 7 5 8 6 10 9 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. D.O.
FUNCTIONAL DESCRIPTION OF HI 964400 THE FRONT PANEL 1. Liquid Crystal Display 2. Keyboard: ALT key to display altitude settings CAL key to enter or exit the calibration mode; to enable or disable the date or time settings; to start or exit the D.O. logging mode CFM key to confirm the calibration values COMM key to display the RS232C baud rate and command prefix DATE key to display the date LOG key to display the lot number on the primary LCD and the page number on the secondary one.
prefix for communication to PC NaCl key to display salinity settings OFF key to turn the meter on off ON key to turn the meter on RANGE key to select dissolved oxygen measurement mode in ppm or in % TEMP key to select temperature measurement mode TIME key to display the time " key to select the calibration value; to set date, time, logging interval; to set the altitude and the salinity factors; to set the baud rate and command prefix for communication to PC. THE REAR PANEL 1. 2. 3. 4. Socket for D.O.
SPECIFICATIONS OF HI 964400 Range D.O. ppm D.O. % Temp. °C 0.00 to 45.00 0.0 to 300.0 0.0 to 50.0 Resolution D.O. ppm D.O. % Temp. °C Accuracy 0.01 0.1 0.1 D.O. Temp. °C ±1.5% of full scale ±0.5 Typical EMC D.O. Deviation Temp. °C ±1.5% of full scale ±0.5 Calibration D.O. single or double point at 0% (HI 7040) and 100% (in air) Temp. °C single point or double point at 0.0°C and/or 50.
LCD FUNCTIONAL DESCRIPTION Primary Display ppm DATE INTV CFM MEM °C WRONG CAL LOG TIME 1 BUF 2 m S Secondary Display OPERATIONAL GUIDE POWER CONNECTION Plug the 12VDC adapter to the meter and to the mains. HI 964400 uses an EEPROM to retain the D.O. calibration and temperature calibration as well as the serial communication setting. The instrument will store the respective data after a calibration or serial communication setting, even when it is not plugged-in.
PROBE CONNECTION & PREPARATION To prepare the instrument for use, connect the D.O. probe to the meter securely by aligning the pins with the socket located on the back of the meter, pushing the plug in and tightening the threaded ring. All probes shipped from Hanna Instruments are dry. To hydrate the probe and prepare it for use, connect it to the meter and proceed as follows. Shipping cap 1. Remove the red and black plastic cap. This cap is for shipping purposes and can be thrown black away. 2.
TURNING THE METER ON To switch the meter on, press the ON key and let the probe in the auto-conditioning (polarization) mode before proceeding. After approximately 5 minutes, the instrument can be calibrated (see page 17). ON ppm If the probe is disconnected, the meter will display "----". °C This also indicates the possibility of a broken probe cable. PROBE POLARIZATION The probe is under polarization with a fixed voltage of approximately 800 mV.
to the meter and properly polarized. For an accurate calibration, it is recommended to wait for 5 or 10 minutes to ensure precise conditioning of the probe. Keep the protective cap on during the polarization time and remove it for the calibration and the measurements. Follow the calibration procedure on page 17. D.O. MEASUREMENTS (in ppm or in %) Make sure the meter has been calibrated (see page 17) and the protective cap has been removed.
Press the RANGE key to change the reading from ppm to % and vice-versa. RANGE For accurate dissolved oxygen measurements a water movement of 0.3 m/sec is required at a minimum. This is to ensure that the oxygen-depleted membrane surface is constantly replenished. A moving stream will provide adequate circulation. The use of a magnetic stirrer to ensure a certain velocity in the fluid is recommended.
Allow the probe to reach the thermal equilibrium before taking any measurement. Reaching thermal equilibrium can take several minutes. The greater the difference between the temperature at which the probe was stored and the temperature of the sample, the longer the time will be. If "----" is displayed, it indicates that the D.O. probe is not properly connected or the temperature is out of range. This also indicates the possibility of a broken probe cable.
Press the RANGE key, the day starts blinking. DATE RANGE Use the UP or DOWN arrow keys to select the day. Press the RANGE key and the year on the secondary LCD will blink. DATE RANGE Use the UP or DOWN arrow keys to select the year. Press the CAL key to exit the date setting mode.
Press the TIME key to display the time. The hour and the minutes will be displayed on the primary LCD, the logging interval on the secondary one. TIME TIME INTV S Press the CAL key to enter the setting mode, the hour will start blinking. TIME CAL INTV S Use the UP or DOWN arrow keys to select the hour. Press the RANGE key and the minutes will start blinking. TIME RANGE INTV S Use the UP or DOWN arrow keys to select the minutes.
Press the RANGE key and the logging interval on the secondary LCD will start blinking (m = minutes, s = seconds). TIME RANGE INTV S Use the UP or DOWN arrow keys to select the logging interval. Press the CAL key to exit the time setting mode. CAL VIEWING THE DATE AND THE TIME Press the DATE key to display the date. The month and the day will be displayed on the primary LCD, the year on the secondary one.
Press the TIME key to display the time. The hour and the minutes will be displayed on the primary LCD, the logging interval on the secondary one. TIME TIME INTV m RESET BUTTON The RESET button is used when the instrument displays erroneous messages due to strong electrical interference or when the instrument's power supply was disconnected before the meter was switched off. It is necessary to press the RESET button and restart the entire operation. Calibration points should remain memorized.
D.O. CALIBRATION For greatest accuracy, it is recommended that the instrument is calibrated frequently. The standard calibration program of the meter is prepared for 2 (maximum) values: 0.0% (zero calibration) and 100.0% (slope calibration). The meter is equipped with a stability indicator and the user will be guided step by step with easy indications on the display during the D.O. calibration. This will make the calibration a simple and error-free procedure.
• • minutes to ensure precise conditioning of the probe. Remove the protective cap from the D.O. probe. Set the appropriate altitude factor (see page 23). Make sure the salinity factor is set to zero (see page 25). ZERO CALIBRATION • Dip the probe into HI 7040 zero oxygen solution and stir gently for 2-3 minutes. • Press the CAL key and the "BUF" indicator will blink until the reading is stable. % CAL BUF • As soon as the reading is stable, the "CFM" indicator will start blinking.
• If the reading is within the margins (±15%), the meter stores the value (and adjusts the offset point). The meter will then proceed with the next calibration point. Press the CAL key and the calibration process is ended CAL with only the zero of the meter calibrated. For a twopoint calibration do not press the CAL key and follow the procedure below. SLOPE CALIBRATION It is suggested to perform the slope calibration in saturated air.
• As soon as the reading is stable, the "CFM" indicator will start blinking. Press the CFM key to confirm the "100.0%" D.O. reading. % CFM CFM • If the reading is not close to the selected value, "WRONG " and "WRONG " will blink alternatively. 1 BUF % % WRONG WRONG BUF • If the reading is within the margins (±15%), the meter stores the value (and adjusts the slope point). The calibration is ended and the meter will then revert to the normal measurement mode.
TEMPERATURE CALIBRATION Each meter has been factory calibrated for the temperature with the D.O. probe supplied and is ready for measurements. The D.O. probes are interchangeable and no temperature calibration is needed should the probe be replaced. If, for any reason, the temperature measurements are out of accuracy, the temperature re-calibration should be carried out. For an accurate re-calibration, contact your nearest Hanna Service Center or follow the procedure below (for technical personnel only).
• Immerse the D.O. probe in the vessel with the ice and water. • Wait for about 30 seconds. Press the CFM key. The secondary LCD section will show "50.0°C". CFM CAL °C • Immerse the D.O. probe in the vessel with hot water. • Wait for about 30 seconds. Press the CFM key. • The temperature calibration procedure is now completed.
ALTITUDE COMPENSATION Press the ALT key and the altitude factor will be displayed. ALT Use the UP and the DOWN keys to set the altitude between 0 and 1900 m, in steps of 100 m (1 meter = 3.28 feet). Altitude affects D.O. concentration decreasing its value. The table on the following page reports the maximum oxygen solubility at various temperatures and altitudes.
Altitude, Meters above Sea Level °C 0m 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 14.6 13.8 13.1 12.4 11.8 11.3 10.8 10.3 9.9 9.5 9.1 8.7 8.4 8.1 7.8 7.5 7.3 7.1 6.8 6.6 6.4 300 m 600 m 900 m 1200 m 1500 m 1800 m 14.1 13.3 12.7 12.0 11.4 10.9 10.4 9.9 9.7 9.2 8.8 8.4 8.1 7.8 7.5 7.2 7.1 6.9 6.6 6.4 6.2 13.6 12.9 12.2 11.6 11.0 10.5 10.1 9.6 9.2 8.7 8.5 8.1 7.8 7.5 7.3 7.0 6.8 6.6 6.3 6.2 6.0 13.2 12.4 11.9 11.2 10.6 10.2 9.7 9.3 8.9 8.6 8.2 7.8 7.5 7.3 7.0 6.8 6.6 6.4 6.1 5.9 5.
SALINITY COMPENSATION Press the NaCl key and the salinity factor will be displayed. NaCl Use the UP and DOWN keys to set the salinity between 0 and 40 g/l. Salinity affects D.O. concentration decreasing its value. Below is a table showing the maximum solubility of oxygen at various temperature and salinity. Salinity (g/l) at Sea Level °C 0 g/l 10 g/l 20 g/l 30 g/l 35 g/l °F 10 12 14 16 18 20 22 24 26 28 11.3 10.8 10.3 9.9 9.5 9.1 8.7 8.4 8.1 7.8 10.6 10.1 9.7 9.3 8.9 8.5 8.2 7.9 7.6 7.4 9.9 9.
LOGGING FUNCTION This function allows to log D.O. (in ppm or %) together with the temperature automatically without the necessity of an operator and for a long period of time. All logged data can be stored into a PC through the RS232C port. The lot number goes from 1 to 99 and then back to #1. The maximum capacity per lot is 8000 samples. Set the appropriate logging interval (see page 12). Select between 1, 15, 30 seconds or 1, 5, 30, 60, 120, 180 minutes. Press the RANGE key first (to select D.O.
Once in the logging mode, the interval cannot be changed. Exit the logging mode first (by pressing the LOG and then CAL key) before setting a new interval. CAL LOG During logging, you can check some information about the logged data. Press the LOG key and the primary LCD will show the current lot number and the secondary LCD will display the current page number. LOG Press the LOG key again to display the current sample number (the number of readings that have been stored in the current lot).
TO STOP LOGGING To stop logging press the LOG and then CAL key. CAL LOG The display will show the next lot number. L LOG 2 OFF Note: if the OFF key is pressed while logging, the meter will stop the logging first and then will turn off. MEMORY ORGANIZATION The memory used for storing the logged data is divided into 16 pages. The capacity of each page is 500 samples. It starts to log from page 16 downwards until 1 and then 16 again, overwriting the previous data.
When the samples collected for a single lot are more than the limit (8000 samples) the meter will stop logging automatically. TO CLEAR LOGGED DATA The entire logged data can be cleared by pressing the ON and the LOG keys simultaneously. ON + LOG The choice has to be confirmed by pressing the CFM key. CFM The next logging will start from page 16.
INTERFACE WITH PC Data transmission from the instrument to the PC is now much easier with the new HI 92000 Windows® compatible application software offered by Hanna Instruments. User friendly, HI 92000 offers a variety of features and has an on line help feature to support you throughout all situations. HI 92000 allows you to use the powerful means of the most diffused spread sheet programs (e.g. Excel©, Lotus 1-2-3©). Simply run your favorite spread sheet and open the file downloaded by HI 92000.
PROBE & MEMBRANE MAINTENANCE The oxygen probe body is made of reinforced plastic for maximum durability. A thermistor temperature sensor provides temperature measurements of the sample tested. It is always recommended that the protective cap be kept on the probe when the probe is not in use to provide protection against damage and dirt. To replace the membrane or refill with electrolyte, proceed as follows: • Remove the protective cap by gently twisting and pulling it off the body of the probe (see fig.
• • with your finger as this will damage the membrane. Make sure that the rubber O-ring sits properly inside the membrane cap. With the sensor facing down, slowly screw the membrane cap clockwise. Some electrolyte will overflow. The Platinum cathode (#8 in the Functional Description at page 3) should always be bright and untarnished. If it is tarnished or stained, which could be due to contact with certain gases or extended use with a loose or damaged membrane, the cathode should be cleaned.
ADDITIONAL INFORMATION (for Technical Personnel only) If you are not using Hanna Instruments HI92000 application software, please find here below some additional information to help your connection to the PC. SETTING THE BAUD RATE AND THE COMMAND PREFIX The transmission speed (baud rate) of your HI964400 and of the external device must be the same. To set the baud rate of the meter press the COMM key, the primary LCD shows the current baud rate.
Press the COMM key to confirm the setting and the primary LCD shows the current command prefix; 16 is the factory setting. COMM Note: the Command Prefix does not have to be changed using HI92000 Hanna Software. Select a different command prefix (between 0 to 47 decimal) by pressing the UP or DOWN arrow keys. Press the COMM key to confirm the setting.
SENDING COMMANDS FROM PC With terminal programs such as Telix® and Windows Terminal®, it is possible to remotely control your HI 964400. Use HI 920010 cable to connect the meter to the PC, start the terminal program and set the communication options as follows: 8, N, 1, no flow control. Command Types To send a command to the D.O.
TM? Causes the meter to send the temperature value. If the reading is out of range "Err 3" is sent. DA? Requests the meter to send the date E.g. "022896" for 28th Feb. 96 TI? Requests the meter to send the time E.g. "233001" for 23:30 hr, 1 sec. as interval "233002" for 23:30 hr, 15 sec. as interval "233003" for 23:30 hr, 30 sec. as interval "233004" for 23:30 hr, 1 min. as interval "233005" for 23:30 hr, 5 min. as interval "233006" for 23:30 hr, 30 min. as interval "233007" for 23:30 hr, 60 min.
4) Channel #1 status E.g. "1" for ppm logging selected in this lot "0" for ppm logging not selected in this lot 5) Channel #2 status E.g. "1" for % logging selected in this lot "0" for % logging not selected in this lot 6) Channel #3 status E.g. "1" for not used "0" for not used 7) Channel #4 status E.g. "1" for temperature logging selected in this lot "0" for temperature logging not selected in this lot 8) ... Repeat from 2 to 7 for the next available lot No.
5) 6) 7) 8) 9) 10) 11) 12) 13) 14) Channel #2 status E.g. "1" for % logging selected in this lot "0" for % logging not selected in this lot Channel #3 status E.g. "1" for not used "0" for not used Channel #4 status E.g. "1" for temperature logging selected in this lot "0" for temperature logging not selected in this lot begin sample time, min E.g. "59" for 59 minute begin sample time, hour E.g. "12" for 12 hour begin sample time, day E.g. "09" for 9th day begin sample time, month E.g.
15) 16) 17) 18) 19) last sample time, hour E.g. "12" for 12 hour last sample time, day E.g. "09" for 9th day last sample time, month E.g. "09" for September last sample time, year E.g. "96" for year 1996 etx end ?DM Requests the meter to send the selected lot data memory. The data are sent in the following order: 1) stx 2) Lot number E.g. "01" for lot No. 1 3) Channel #1 status E.g. "1" for ppm logging selected in this lot "0" for ppm logging not selected in this lot 4) Channel #2 status E.g.
8) begin sample time, hour E.g. "12" for 12 hour 9) begin sample time, day E.g. "09" for 9th day 10) begin sample time, month E.g. "09" for September 11) begin sample time, year E.g. "96" for year 1996 12) logging interval E.g. "0" for 1 second "1" for 15 seconds "2" for 30 seconds "3" for 1 minute "4" for 5 minutes "5" for 30 minutes "6" for 60 minutes "7" for 120 minutes "8" for 180 minutes 13) Total number of samples per lot E.g. "1234" for total no. of samples: 1234.
15) last sample time, min E.g. "59" for 59 minute 16) last sample time, hour E.g. "12" for 12 hour 17) last sample time, day E.g. "09" for 9th day 18) last sample time, month E.g. "09" for September 19) last sample time, year E.g. "96" for year 1996 20) etx end The meter will send "Err6" if in a different measurement range. Commands setting parameters: /ML To select the data lot for data transfer. E.g. send "/ML05" to select lot no. 5. If the lot no.
/PF To set the RS232C command prefix. E.g. send "/PF05" to set the command prefix to 05. Note: will be sent by the meter if the command received is accepted, otherwise it will send . equals to ASCII code 06 and equals to ASCII code 24. If sample data is out of range "07FFFH" is sent. These commands may be sent with either capital or small letters. Invalid commands will be ignored. The characters sent by the pH meter are always capital letters.
ACCESSORIES ChecktempC Electronic termometer (range: -50.0 to 150.0°C) ChecktempF Electronic termometer (range: -58.0 to 302°F) HI 7040M Zero Oxygen Solution, 230 ml HI 7040L Zero Oxygen Solution, 460 ml HI 7041S Refilling Electrolyte Solution, 30 ml HI 710005 115VAC to 12VDC converter HI 710006 230VAC to 12VDC converter HI 76407/2 Spare probe with 2 meters (6.
WARRANTY All Hanna Instruments are warranted for two years against defects in workmanship and materials when used for their intended purpose and maintained according to the instructions. The probes are warranted for a period of six months. Damages due to accident, misuse, tampering or lack of prescribed maintenance are not covered. This warranty is limited to repair or replacement free of charge of the meter only, whenever due to defect of manufacturing.
CE DECLARATION OF CONFORMITY DECLARATION OF CONFORMITY We Hanna Instruments Srl V.le delle industrie 12 35010 Ronchi di Villafranca (PD) ITALY herewith certify that the bench D.O. meter HI 964400 has been tested and found to be in compliance with the following regulations: IEC 801-2 IEC 801-3 IEC 801-4 EN 55022 Electrostatic Discharge RF Radiated Fast Transient Radiated, Class B Date of Issue: 18-04-1996 D.Volpato - Engineering Manager On behalf of Hanna Instruments S.r.l.
PRINTED IN ITALY MANDOBNR1 02/00 h t t p : / / w w w . h a n n a i n s t .
