Instruction Manual HI 84529 Titratable Acidity Mini Titrator & pH Meter for Dairy Products
Dear Customer Thank you for choosing a Hanna Instrument product. Please read this instruction manual carefully before using this instrument. This manual will provide you with the necessary information for correct use of this instrument, as well as a precise idea of its versatility. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com or view our worldwide contact list for a Hanna representative near you at www.hannainst.com. All rights are reserved.
Included ....................................................................................................................... 4 Safety Measures .........................................................................................................5 Table of Contents Description .............................................................................................................. 6-8 Significance of Use ...........................................................................................
Included Please examine this product carefully. Make sure that the instrument is not damaged. If any damage occurred during shipment, please notify your local Hanna Representative.
Before using this product, make sure that it is entirely suitable for your specific application and for the environment in which it is used. Safety Measures Operation of this instrument may cause unacceptable interferences to other electronic equipment, thus requiring the operator to take all necessary steps to correct interferences. Any variation introduced by the user to the supplied equipment may degrade the instrument’s EMC performance.
Description The HI 84529 is a low-cost easy to use, microprocessor-based automatic minititrator and pH meter designed for the rapid and accurate analysis of Total Titratable Acidity in Dairy Products. The HI 84529 will quickly become a valuable tool by eliminating subjective factors including color indicators, errors in calculations or erratic titrant additions. The instrument benefits from Hanna’s many years of experience as a manufacturer of quality analytical instrumentation.
Thorner degrees (°Th) - mostly used in Sweden and the CIS. This value is obtained by titrating 100 mL of milk, diluted with 2 parts of distilled water, with 0.1N NaOH, using phenolphtalein as indicator. Significance of Use Dornic degrees (°D) - mostly used in Netherlands and France. This value is obtained by titrating 100 mL of milk, with N/9 (0.11 N) NaOH, using phenolphtalein as indicator. Percent lactic acid (%l.a.) - frequently used in the UK, USA, Canada, Australia and New Zealand.
Principle of Operation In an ideal solution, the endpoint of an acid titration corresponds stoichiometrically to the complete neutralization of the acids present. This endpoint for this reaction can be determined visually using a color indicator (i.e. phenolphthalein), however this endpoint becomes very objective in opaque and colored samples. The HI 84529 removes this issue by titrating to a fixed pH endpoint that can be set by the user.
Product Diagram Overhead View 1. Titrant bottle 2. Liquid Crystal Display (LCD) 3. Keypad 4. Electrode holder 5. Dispensing tube Front View www.hannainst.
Product Diagram 1 15 2 3 4 5 Rear View 6 14 7 13 12 8 9 1. 2. 3. 4. 5. 6. 7. 8. 10 Product Diagram Dispensing tube pH Electrode Temperature probe Reference electrode Syringe Electrode holder Power switch USB connector (PC interface) 10 11 9. 10. 11. 12. 13. 14. 15. USB connector (Storage interface) Power adapter BNC electrode connector Temperature connector Reference connector Titrant bottle Dosing Pump Valve www.hannainst.
Keypad Function ESC - used to leave the current screen and to return either to the previous screen or to the main screen. In Setup, exits a parameter without changing the value / - used to modify the parameters’ values, to scroll the information displayed while viewing a help screen or to move between the options from the instrument’s Setup CAL - used to access the Electrode and Pump calibration options HELP - used to access/exit the instrument’s contextual help www.hannainst.
Guide to Indicators During the instrument’s operation a set of information are displayed on the LCD. Displayed icons: Stirrer on Pump running Stirrer is not working properly Unstable reading Parameter can be modified 1. Current time and instrument mode information (pH meter or Titrator) 2. Procedural Indicators 3. Instrument status 4. Virtual option keys 5. Stirrer and reading status 6. Main reading information 7. pH temperature compensation mode (Manual or Automatic) 8.
The dosing pump is based on a valve that automatically moves the titrant between the titrant bottle and syringe when filling the syringe and between the syringe and sample when dispensing. A replaceable 5 mL plastic syringe is used to limit the amount of titrant used per test to ensure the highest possible accuracy. Before a set of titrations, it is necessary to prime the dosing system.
Startup Menu The titrator’s setup menu may be accessed from the main screen or titration screens (meter or titrator) by pressing the MENU key, then SETUP. A list of setup parameters will be displayed with currently configured settings. While in the setup menu, it is possible to modify the instrument’s operation parameters. The ARROW keys permit the user to scroll the setup parameters. Press HELP to view the contextual help. Press ESC to return to the main screen. Concentration Unit % l.a.
Use the ARROW keys to select the value. Press Accept to confirm or ESC to return to the setup menu without saving the changes. Startup Menu pH Resolution 0.1 or 0.01. Press the displayed virtual option key to change the option. Temperature Unit °C, °F or K. Press the virtual option key to change the option. Time Press the Modify key to change the time and time format Press Format to switch between 12 hour (am/pm) and 24 hour mode. Press the arrow to highlight the value to be modified.
Startup Menu Date Press the MODIFY key to change the date and date format. Key Beep Select ENABLE to activate or DISABLE to deactivate the Key Beep function. If enabled, a short beep will be heard every time a key is pressed. Error Beep Select ENABLE to activate or DISABLE to deactivate the Error Beep function. If enabled, a beep will be heard when an error condition occurs. Decimal Separator Select the symbol used for a decimal separator. You can chose either period or a comma.
Use the ARROW keys or ← / → to increase/ decrease the value. Press ACCEPT to confirm the value or ESC to return to the setup menu. Startup Menu LCD Backlight Press Modify to change the backlight level. The default value is 3. Use the ARROW keys or ← / → to increase/ decrease the backlight level. Press ACCEPT to confirm or ESC to return to the setup menu. pH endpoint Press Modify to change the pH endpoint value. The default value is 8.3 pH. Use the ARROW keys to select the value.
Startup Menu Language Press the corresponding virtual option key to change the language. If the selected language cannot be loaded, the previously selected language will be reloaded. If no language can be loaded at startup, the instrument will work in “safe mode”. In “safe mode” all messages are displayed in English. Tutorial ENABLE or DISABLE the Tutorial. This helpful tool offers additional information that guides the user step by step during calibration and titration.
Press YES to confirm the restore process or NO to return without restoring. Press ESC to return to the Setup mode. This screen appears when the instrument is turned on during the initialization process. Startup Menu Guide to Display Codes Titration screen display. Titration screen when a titration is in progress. Prime burette screen. www.hannainst.
Guide to Display Codes Prime burette screen when the dosing system is running. This error message appears when the pump is not working properly. Check the tubing, valve and syringe. Press RESTART to try again This screen appears when the titrator is in calibration mode. Press PUMP to calibrate Pump. Press ELECTRODE to calibrate pH electrode. Pump Calibration Messages Pump calibration is initiated by pressing the START key. This screen appears while pump calibration is in progress.
This error message appears during pump calibration when the endpoint cannot be reached and the maximum amount of titrant is exceeded. Check standard, electrode and/or dosing system and try again Pump Calibration Messages The calibration was outside the acceptable limits. Prepare a new standard and try again. This error message appears when the pH reading exceeds the acceptable input limits (pH <-2.00 or pH> 16.00). This screen appears when the stirrer is not working properly.
pH Calibration Messages pH calibration mode. When the reading has stabilized, press CONFIRM to accept the calibration or CLEAR to restore the default calibration. The “Wrong Buffer” message is displayed when the pH value is outside of the acceptable range. Clean the electrode by following the cleaning procedure and/or check the buffer value before continuing the pH calibration. Press the ESC key to exit pH calibration mode.
This error message appears when the input reading (pH or temperature) exceeds the specified limits. The pH or temperature value and concentration will blink indicating an error. Titration Messages This screen appears when the sample concentration is out of range. This screen appears when the stirrer is not working properly. Check the stir bar and beaker content. Press RESTART to try again. This error message appears when the pump is not working properly. Check the tubing, valve and syringe.
Electrode Preparation Procedure 24 Remove the electrode protective cap. If the bulb is dry, soak the electrode in HI 70300 Storage Solution for at least one hour. Preparation of reference electrode: 1. Unwrap Parafilm™ seal found over ceramic junction on inner stern of the reference electrode and discard. This is only used for shipping. 2. Rinse inner stern with deionized water, making certain to wet o-ring found on the inner stern. 3.
Immerse the pH and reference electrodes 1.5 cm (0.6”) in the sample and stir gently for a few seconds. For a faster response and to avoid cross-contamination of the samples, rinse the electrodes with a few drops of the solution to be tested, before taking measurements. Electrode Preparation Procedure It is recommended to calibrate the instrument frequently, especially if high accuracy is required. The pH electrode should be recalibrated: 1. Whenever the pH electrode is replaced 2.
Electrode Calibration Procedure 1 Point Calibration 1. The 8.30 buffer will be selected by default. If necessary, press the ARROW keys in order to select a different buffer value. 2. The (unstable measurement) symbol will be shown on the display until the reading becomes stable. 3. When the reading is stable and close to the selected buffer, the (unstable measurement) symbol will disappear and the CONFIRM key will become active. 4. Press CONFIRM to confirm the calibration or ESC to exit calibration. 5.
buffer value will be automatically selected. 14. After the second calibration point has been confirmed, press ESC to exit without performing the third calibration point. 3 Point Calibration 1. Remove the electrode holder with electrode from the top of the beaker. 2. Place the third beaker with calibration buffer in the beaker holder. Rinse the electrodes in a beaker containing the third buffer rinsing solution. 3.
Electrode Calibration Procedure If the computed slope isn’t within the preset limits, the meter will display the message “Wrong Slope”. If the slope is too high the symbol will be displayed. If symbol will the slope is too low the be displayed. If the “Wrong Old Slope” error message is displayed, an inconsistency exists between the current and the previous (old) calibration. Clear the previous calibration by pressing CLEAR and proceed with calibration from the current calibration point.
Note: To clear a previous calibration and to return to the default value, press CLEAR at any time after entering calibration mode. If CLEAR is invoked during the first calibration, point the instrument returns to the measurement mode. The CLEAR key is displayed only if a previous calibration exists. Electrode Calibration Procedure Temperature has an effect on pH. The calibration buffer solutions are affected by temperature changes to a lesser degree than normal solutions.
Dosing Pump Installation To install the dosing pump follow the procedure below: 1. 2. 3. 4. 5. 6. 7. Dosing Pump Prime Procedure Extend the plunger on the 5 mL syringe to its maximum volume. Place the syringe in the dedicated spot on the top of the meter (1). Arrange the bottom of the syringe into the holder on the pump (2). Once the syringe is in place lower the barrel until it sits flush on the holder.
Dosing Pump Prime Procedure Note: The aspiration tube must be inserted in the titrant bottle. The dosing tip must be placed over a rinse beaker. Before starting the prime procedure, make sure you are using the appropriate titrant solution for the selected range. To prime the burette, select PRIME option from TITRATION mode. Adjust the rinses number by pressing the and keys and press START.
Dosing Pump Prime Procedure NOTE: This error message appears when the pump is not working properly. Check the tubing, valve and syringe. Press RESTART to try again. Pump Calibration Procedure The calibration of the pump must be performed every time the syringe, pump tube, the titrant bottle or the pH electrode is changed. It is recommended to perform the pump calibration before each set of titrations, after the titrator is left idle for several hours, or once daily.
NOTE: DO NOT PLACE THE DISPENSING TIP INTO THE CALIBRATION BEAKER, PLACE THE TIP OVER A WASTE BEAKER. A SMALL AMOUNT OF TITRANT IS DISPENSED WHEN THE PUMP RESETS. Pump Calibration Procedure Press START, wait for the syringe to refill. Place the stir bar in the beaker and put the beaker in the minititrator top. Place the probe holder on the top of the beaker and secure it by turning clockwise. Rinse the pH electrode, reference electrode and temperature probe with deionized water and immerse them roughly 1.
Pump Calibration Procedure If an erroneous situation is encountered during the calibration, an error message is displayed and the calibration can be restarted by pressing RESTART. Prepare a new standard, rinse the electrode, temperature probe and dosing tip and try again. If the calibration doesn’t complete and the max titrant volume of titrant is reached, an error message will be displayed. The calibration can be restarted by pressing RESTART.
For best accuracy, before taking any measurement, ensure that the pump is calibrated on the selected range following the “Pump Calibration Procedure”. Note: Verify that the instrument has been calibrated (pH and pump) before performing any titrations. Refer to “Setup Menu” to set up the instrument for your measurement. Select the corresponding measurement range. Unit Low Range 20 and 50 %l.a 0.01 to 0.20 0.1 to 2.0 °SH 0.4 to 8.9 4.4 to 88.9 °D 1.0 to 20.0 10 to 200 °TH 1.1 to 22.2 11.1 to 222.
Titration Procedure For 20 g and 20 mL samples add deionized or distilled water to the 60 mL mark. Place the stir bar in the beaker and put the beaker in the mini titrator top. Place the probe holder on the top of the beaker and secure it by turning clockwise. Rinse the pH electrode, reference electrode and temperature probe with deionized water and immerse it roughly 1.5 cm (0.6”) into the sample. Be sure that the tip of the electrode is not hitting the stir bar.
At the end of the titration, the concentration is displayed in the selected unit. The titration curve can be viewed by pressing Plot ON. Press Plot OFF or ESC to exit this mode. Titration Procedure Press LOG to record the concentration value and the titration curve into the instrument’s memory. A message will be displayed for a few seconds indicating the amount of free log space. Up to 200 log samples can be recorded in the instrument’s memory.
Titration Procedure This screen appears when the stirrer is not working properly. Check the stir bar and beaker content. Press RESTART to try again. This error message appears when the pump is not working properly. Check the tubing, valve and syringe. Press Restart to try again. Tips for an Accurate Measurement The instructions listed below should be followed carefully to ensure measurements are conducted with the highest possible accuracy and precision.
text format files on the storage device. Press METER or TITRATOR to view the respective logs. The instrument will display a list of all the records stored in the log. Use the ARROW keys to scroll the list of records. If the saved concentration was out of range, the “<” or “>” symbols are displayed in front of the reading. Press DELETE to delete the selected log from the memory. Press DEL.ALL to delete all records. Press INFO to see detailed information about the highlighted record.
Press MENU then GLP (Good laboratory Practice). Titrator GLP Information From this screen it is possible to select ELECTRODE or the PUMP GLP. Press PUMP to view the pump’s last calibration date, time, and slope. If a calibration hasn’t been performed, the message “Not Calibrated” will be displayed. pH Measurement 40 The HI 84529 can be used as a pH meter for direct measurements. Verify that the instrument has been calibrated before taking pH measurements. Set the instrument to pH meter.
If CAL DUE is displayed perform an electrode calibration. Options: Press MENU to access the instrument’s menu. Press HELP to view the contextual help whenever time you need additional information. Press STIR to start/stop the stirrer. Press TITRATOR to enter titration mode. Press CAL to access the calibration menu. Press LOG to save the current reading. A message indicating the free log space will be displayed for a few seconds.
pH Measurement display. Automatic Temperature Compensation will provide pH corrected values for the measured temperature. If Manual Temperature Compensation (MTC) is desired, the temperature probe must be disconnected from the instrument. The default temperature of 25 °C (77 °F) or the last temperature reading will be displayed, preceded by the symbol and the “MTC” message. The temperature can be adjusted with the ARROW keys (from -20.0 to 120.0 °C).
If the pH log is empty, the message “No records available!” will be displayed. pH Measurement The pH meter GLP screens displays the last pH calibration data. To view this information press MENU key then GLP. Press ELECTRODE to view information regarding electrode calibration. pH Meter GLP Information The following items are included in electrode GLP: the time and date of the last calibration, offset, slope, electrode condition, calibration timeout and the calibration buffers.
Data stored on the meter with the LOG function during mV measurement and titrations can be transferred from the meter to a USB stick using the Export function from the log recall menu.Two text files are transferred on the USB stick. These files can be used for further analysis on a PC. The logged data can also be transferred from the instrument to the PC using a USB cable.Connect the USB cable and the following screen will be displayed. Press METER to generate the text file with Meter log data.
Storage Procedure To assure a quick response time, the glass bulb should be kept moist and not allowed to dry out. Replace the solution in the protective cap with a few drops of HI 70300 or HI 80300 Storage Solution. The HI 5315 Reference may be stored with its black cap and Fill hole covered. Rinse and refill before using. Follow the Preparation Procedure on page 20 before taking measurements. Electrode Conditioning and Maintenance Periodic Maintenance Inspect the electrodes and the cables.
Troubleshooting Guide 46 Maintenance Symptoms Problem Solution Slow response/excessive drift. Dirty pH or reference electrode. Soak the electrode tip HI 70640 solution for 30 minutes and follow the cleaning procedure. Refill reference electrode with new fill solution. Reading fluctuates up and down (noise). Clogged/dirty junction. Low electrolyte level (reference electrode). Cable connection. Clean the pH electrode. Refill reference electrode with fresh fill solution.
Symptoms Problem Solution After a titration the following is displayed blinking: Low Range: 0.20 %I.a., 8.9 °SH, 20.0 °D or 22.2 °TH. High Range: 0.1 %l.a., 4.4 °SH, 10 °D or 11.1 °TH. Broken electrode. Instrument not calibrated. Wrong sample. Concentration out of range. Check/clean the electrodes. Recalibrate the instrument (pump and pH). Use care during sample preparation. Check sample size and permitted range. At startup, the meter displays the Hanna logo permanently. One of the keys is stuck.
Specifications Titrator Range (Low Range): %l.a.: 0.01 to 0.20; ºSH: 0.4 to 8.9; ºD: 1.0 to 20.0; ºTH: 1.1 to 22.2 (High Range): %l.a.: 0.1 to 2.0; ºSH: 4.4 to 88.9; ºD: 10 to 200; ºTH: 11.1 to 222.2 Resolution (Low Range): %l.a.: 0.01; ºSH: 0.1; ºD: 0.1; ºTH: 0.1 (High Range): %l.a.: 0.1; ºSH: 0.1; ºD: 1; ºTH: 0.1 Accuracy (@25ºC/77ºF) (Low Range): ± 0.01 %l.a. (High Range): ± 0.1 %l.a. Method Acid-base titration Principle Endpoint titration, 8.30 pH adjustable pH 8.1 - 8.
Specifications Temperature Range -20.0 to 120.0°C (-4.0 to 248.0°F) Resolution 0.1°C Accuracy ±0.4°C without probe error Additional Specifications pH Electrode FC 260B pH electrode with 1 m (3.3’) cable (included), Reference Electrode HI 5315 reference probe with 1 m (3.3’) cable (included) Temperature Probe HI 7662-M stainless steel temperature probe with 1 m (3.
Accessories Reagents HI 84529-50 Titrant solution for Low Range 20 (120 ml) HI 84529-51 Titrant solution for High Range 20 (120 ml) HI 84529-52 Titrant solution for Low Range 50 (120 ml) HI 84529-55 Pump Calibration Standard (230 mL) pH Calibration Solutions HI 7004M Buffer solution pH 4.01 (230 mL) HI 70060M Buffer solution pH 6.00 (230 mL) HI 70083M Buffer solution pH 8.30 (230 mL) HI 7010M Buffer solution pH 10.
Electrodes Accessories FC 260B pH electrode HI 5315 Reference electrode HI 7662-M Temperature probe Other Accessories HI 70500 Tube set with cap for titrant bottle, tip and valve HI 71005/8 115 Vac to 12 Vdc, 800 mA HI 71006/8 230 Vac to 12 Vdc, 800 mA HI 731319 Stir bar, 25 x 7 mm (10 pcs.) HI 740036P 100 mL Beaker (10 pcs.) HI 740037P 20 mL Beaker (10 pcs.) HI 740236 5 mL syringe for minititrator HI 920013 PC Connection Cable www.hannainst.
Warranty HI 84529 is guaranteed for two years against defects in workmanship and materials when used for it’s intended purpose and maintained according to instructions. Electrodes and probes are guaranteed for six months. This warranty is limited to repair or replacement free of charge. Damage due to accidents, misuse, tampering or lack of prescribed maintenance is not covered. If service is required, contact your local Hanna Representative from whom you purchased the instrument.
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contacts Local Office Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 www.Hannainst.com Hanna Instruments USA 270 George Washington Highway Smithfield, RI 02917 Phone: 800.426.6287 Fax: 401.765.7575 e-mail: tech@Hannainst.
