Instruction Manual HI 84429 TITRATABLE ACIDITY MINITITRATOR & pH METER for Dairy Products w w w. h a n n a i n s t .
Dear Customer, Thank you for choosing a Hanna product. This manual will provide you with the necessary information for the correct use of the instrument. Please read it carefully before using the meter. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com. TABLE OF CONTENTS PRELIMINARY EXAMINATION................................................................................................. 3 GENERAL DESCRIPTION .................................................
PRELIMINARY EXAMINATION Please examine this product carefully. Make sure that the instrument is not damaged. If any damage occured during shipment, please notify your Dealer. Each HI 84429 minititrator is supplied complete with: • FC 260B pH electrode • HI 5315 Reference electrode • HI 7072 Filling solution (30 mL) • HI 7662-M Temperature probe • HI 84429-50 Titrant (100 mL) • HI 84429-55 Standard (500 mL) • HI 700640 Cleaning solution for milk deposits (2x20mL) • pH 4.01 buffer solution (230 mL) • pH 6.
GENERAL DESCRIPTION The HI 84429 is an easy to use microprocessor-based automatic minititrator and pH meter designed for the rapid and accurate analysis of Total Titratable Acidity in milk. By eliminating subjective factors including color indicators, errors in mathematical calculations or erratic titrant additions from the measurement, the HI 84429 makes Total Titratable acidity analysis precisely and totally impartially. This will quickly become a valuable analysis tool in your dairy lab.
Titratable acidity measures the total titratable acidity and differs from pH as it also includes the buffering capacity of the milk constituents. Titratable acidity in dairy products, is determined by titrating a sample with sodium hydroxide to a fixed endpoint pH (pH 7.00 or a phenolphtalein endpoint of pH 8.30). The actual neutralization of milk occurs at an endpoint of pH 7.00, however, standard methods utilize the phenolphtalein endpoint value.
SPECIFICATIONS Titrator Range Titratable acidity (low range) °SH: 0.0 to 15.0 °SH °Th: 0 to 40 °Th °D: 0 to 35 °D %I.a.: 0.00 to 0.35 %I.a. Titratable acidity (high range) °SH: 10.0 to 75.0 °SH °Th: 20 to 200 °Th °D: 20 to 175 °D %I.a.: 0.0 to 2.0 %I.a. Resolution Titratable acidity (low range): 0.1 °SH 1 °Th 1 °D 0.01 %I.a. Titratable acidity (high range): 0.5 °SH 1 °Th 1 °D 0.1 %I.a. Accuracy 5% of reading Titration method Acid-base titration Principle End point titration, 8.30 pH Pump debit 0.
Temperature Range Resolution Accuracy -20.0 to 120.0 °C (-4.0 to 248.0 °F) 0.1 °C ±0.4 °C without probe error Electrode FC 260B pH electrode (included) HI 5315 reference electrode (included) Temperature Probe HI 7662-M (included) Environment 0 to 50 °C (32 to 122 °F); max 95% RH non-condensing Power supply 12 Vdc power adapter Dimensions 208 × 214 × 163 mm (8.2×8.4×6.4”) (with beaker) Weight 2200 g (77 oz.) REQUIRED REAGENTS Code HI 84429-50 HI 84429-55 Description Titrant Standard Quantity/Test 0.
PRINCIPLE OF OPERATION A neutralization titration is one where an acid and base react to form a neutral pH solution. H++OH¯→H2O A potentiometric acid-base titration employs an indicator electrode (such as a pH sensor) to establish the equivalence point of the titration. The accepted methodology for Titratable Acidity in dairy products employs a neutralization titration. Acids (such as lactic acid) in the milk products react with a base (the titrant sodium hydroxide) to produce water.
FUNCTIONAL DESCRIPTION FRONT PANEL 1. Titrant bottle 2. Graphic LCD 3. Keypad 4. Electrode holder 5. Peristaltic pump tubes REAR PANEL 6. 7. 8. 9. pH Electrode Temperature probe Reference electrode Beaker 10. Power adapter connector 11. Reference electrode socket 12. BNC electrode connector 13. Temperature probe socket 9 14. Power switch 15.
KEYPAD FUNCTION AND INDICATORS - used to leave the current screen and to return either to the previous screen or to the main screen, depending on the context, while pressed in SETUP the new value of the set parameter is not changed.
GUIDE TO INCDICATORS During the instrument’s operation a set of information are displayed on the LCD. Displayed icons: Pump activity. Unstable reading. Stirrer on. Parameter can be changed. 1 9 2 8 3 1. 2. 3. 4. 5. 6. 7. 8. 9.
PERISTALTIC PUMP Peristaltic pumps are self priming. Liquid never contacts the pump components. The titrant tubing is pressed along the rotating rollers of the pump. The rollers compress the tubing drawing titrant along and out the titrator tip. TITRATOR STARTUP • • • • • • • • • • • • • • Place the instrument on a flat table. Do not place the instrument in direct sun light. Connect the power adapter to the instrument. Turn the instrument ON using the power switch from the rear panel of the instrument.
SETUP CONFIGURATION MENU The titrator’s setup configuration menu may be accessed from the pH or titration screens by pressing the MENU key, then Setup. A list of setup parameters will be displayed with currently configured setting. While in the setup menu it is possible to modify the instrument’s operation parameters. The ARROW keys permit the user to scroll the setup parameters. Press HELP to view the contextual help. Press ESC to return to the main screen. Concentration unit Option: °SH, °Th, °D, % l.a.
Calibration timeout Option: Disabled or 1 to 7 days. This option is used to set the number of days before the pH calibration expired warning message is flagged. Press Modify to access the calibration timeout value modify parameter. Use the ARROW keys in order to increase/decrease the value. Press Accept to confirm or ESC to return to the setup menu without saving the new value. pH resolution Option: 0.1 or 0.01. Press the displayed function key in order to change the option.
Use the ARROW keys or / in order to increase/decrease the displayed constrast. Press Accept to confirm or ESC to return to the setup menu. Contrast Option: 0 to 20. This option is used to set the display’s contrast. Press Modify to change the display’s contrast. Use the ARROW keys or / in order to increase/decrease the value. Press Accept to confirm the value or ESC to return to the setup menu. Date / Time This option is used to set the instrument’s date and time. Press Modify to change the date/time.
Time format Option: AM/PM or 24 hours. Press the functional key to select the new value. Date format Press Modify to change the Date Format. Use the ARROW keys to select the desired format. Press Accept to confirm the value or ESC to return to the setup menu. Language Press the corresponding function key to change the option. If the new selected language cannot be loaded, the previously selected language will be reloaded. If no language can be loaded the instrument will work in the “safe mode”.
Beep On Press the function key to select the new option. When enabled, a short beep is heard every time a key is pressed or when the calibration can be confirmed. A long beep alert sounds when the pressed key is not active or a wrong condition is detected while in calibration. Meter information Press Select to view the firmware version, language version, mV factory calibration date and time and temperature factory calibration date and time. Press ESC to return to the Setup mode.
ELECTRODE PREPARATION PREPARATION PROCEDURE Remove the electrode protective cap. DO NOT BE ALARMED IF ANY SALT DEPOSITS ARE PRESENT. This is normal with electrodes and they will disappear when rinsed with water. During transport tiny bubbles of air may have formed inside the glass bulb. The electrode cannot function properly under these conditions. These bubbles can be removed by "shaking down" the electrode as you would do with a glass thermometer.
ELECTRODE CALIBRATION PROCEDURE It is recommended to calibrate the instrument frequently, especially if high accuracy is required. The pH range should be recalibrated: a) Whenever the pH electrode is replaced b) At least once a week c) After testing aggresive chemicals and after electrode is cleaned d) When extreme accuracy is required e) If the pH calibration expired warning is displayed during measurement.
THREE POINT CALIBRATION • From the pH meter/titrator calibration menu screen select the Electrode calibration option. The electrode calibration screen will be displayed. • The 6.00 buffer will be selected by default. If necessary press the ARROW keys in order to select a different buffer value. • The (unstable measurement) symbol will be shown on the display until the reading becomes stable.
• When the reading is stable and close to the selected buffer, the (unstable measurement) symbol will disappear and the CFM key will become active. • Press CFM to confirm the calibration. • The calibrated value will be shown on the display and the third expected buffer value will be automatically selected. • After the second calibration point has been confirmed, press STIR to stop stirring. • Remove the electrode holder with electrodes from the top of the beaker.
• If the computed slope isn’t within the preset limits the meter will display the message will be displayed. If the slope is “Wrong Slope”. If the slope is too high the symbol will be displayed. too low the symbol • If the “Wrong Old Slope” error message is displayed, an inconsistency exists between the current and the previous (old) calibration. Clear the calibration parameters by pressing Clear and proceed with calibration from the current calibration point.
Notes: • To clear a previous calibration and to return to the default value, press Clear at any time after entering calibration mode. The “Calibration cleared” message will be shown for a few seconds on the display. If Clear is invoked during the first calibration point the instrument returns to the measurement mode. • The Clear key is displayed only if a previous calibration exists. pH BUFFER TEMPERATURE DEPENDENCE The temperature has an effect on pH.
PUMP TUBE INSTALLATION To mount the new peristaltic pump tube follow next steps: • Position one peristaltic pump fixing ring on its location. • Stretch the tube over the peristaltic pump cylinders. • Fix the second pump fixing ring on its location. • Attach the tube to the reagent bottle and to the dosing tip. Note: Purge the peristaltic pump until drops of reagent appears on the dosing tip by pressing the Purge key from the titrator main screen.
Press CAL to access the titration calibration menu where electrode and pump calibration may be accessed. Press HELP to view the contextual help. • Press Continue or ESC to skip the message and enter Titrator main screen. • Press Purge to begin a purge cycle. The purging stops automatically after 5 minutes. To stop purging at any time and return to the main screen press ESC or Stop. During a purge, the remaining time until the purge process will be completed is shown on the lower right side of the display.
After the 5 minutes purging interval has elapsed the “Completed” message is displayed. Another purge period can be initiated by pressing Restart. PUMP CALIBRATION PROCEDURE The calibration of the pump must be performed each time the pump tube, the reagent bottle or the pH electrode is changed. It is recommended to perform the pump calibration before each set of measurements. Verify: The electrode has been calibrated in 8.30 pH buffer.
• From the titrator main screen press CAL. The instrument displays the date and time of the last electrode calibration, and the date and time of the last pump calibration, or calibration expired messages. • Press Pump. The next screen will be displayed. • Press Start. • After the pump calibration is started, on the upper right side of the display two animations will be shown in order to indicate that the pump and the stirrer are working.
• After the pump calibration is complete a confirmation message is displayed for a few seconds, then the instrument will return to the titrator calibration menu and will display the new time and date for the last pump calibration. Notes: • The calibration of the pump is independent of the selected range and concentration unit. • If an erroneous situation is encountered during the calibration, an error message is displayed and the calibration can be restarted by pressing Restart.
TITRATION PROCEDURE The instrument has been calibrated (pH and pump) before performing a titration. An electrode calibration in 8.30 pH buffer is recommended. • Refer to Setup Configuration Menu (see page 13) to set up instrument for your measurement. • Select the corresponding Low Range or High Range according to the table bellow. Verify: U NI T Low Range (50 mL milk sample) High Range (10 mL milk sample) °SH 0.0 to 15.0 10.0 to 75.0 °TH 0 to 40 20 to 200 °D 0 to 35 20 to 175 %l.a. 0.00 to 0.
• From the titrator main screen press Titration. To enter titrator main screen from pH meter mode press Titrator and then Continue. • Press Start to begin the titration process. • After the titration is started on the upper right side of the display two animations will be shown in order to indicate that the pump and the stirrer are running. On the lower right side of the display is shown the period of time since the titration has been started.
• If the concentration is out of limits (15 SH for Low Range, 10 or 75 SH for High Range - see page 6 for range limits) the exceeded range limit will be displayed blinking and the message “Out of range” will be shown. Another titration can be initiated by pressing Restart. Note: If the end-point is not reached or it is not recognized or the input reading is out of 3.00 to 8.30 pH range, an error message will be displayed. The titration can be restarted after a new sample is prepared by pressing Restart.
The instrument will display a list of all the titration records stored in the titration log. Use the ARROW keys to scroll the stored records list. If the saved concentration was out of range the “!” symbol is displayed in front of the reading. Press Delete to enter record deleting mode. Press Delete All to enter all records deleting mode. Press More to view more information. Press Unit to convert the result to other unit. Use the ARROW keys when is displayed to scroll between the log records.
If the titrator log is empty the message “No Records!” will be displayed. TITRATOR GLP INFORMATION Press MENU while in Titration mode and then GLP. From this screen it is possible to select between viewing the electrode GLP or the pump GLP. Press GLP elec. to view the electrode’s last calibration parameters and date. Press GLP pump to view the pump’s last calibration time and date. If GLP elec. is pressed one of the next screens will be displayed.
If GLP pump is pressed, one of the next screens is displayed. The pump GLP displays the Time and Date of the last pump calibration. If a calibration hasn’t been performed the message “Not Calibrated” will be displayed. pH MEASUREMENT The HI 84429 may be used as a pH meter for direct measurements. Verify that the instrument has been calibrated before taking pH measurements. Set the instrument to pH meter. At power up the instrument enters pH meter mode.
Press LOG to memorize the current reading. A message indicating the free log space will be displayed for a few seconds. Up to 50 log samples can be saved into the instrument’s memory. When the free pH log space is under 12% a message will be permanently shown on the display, indicating the remaining free log space. In order to take pH measurements follow the next steps: • Submerge the tip of the electrode (4 cm/1 1/2’’) and the temperature probe into the sample to be tested and stir gently.
VIEW/DELETE pH MEMORIZED SAMPLES Press MENU key while in pH meter screen. Press Recall to access the pH recall. A list of records is stored in the pH log. Use the ARROW keys to scroll the list of records. Press More to see detailed information about focused record. Press Delete to enter record deleting mode. Press Delete All to enter all records deleting mode. If More is pressed a complete set of data is displayed. Use ARROW keys when is displayed to scroll between the records.
If Delete All was pressed the instrument will ask for confirmation. Press CFM to delete all records or ESC to return to the previous screen without deleting. If the pH log is empty the message “No Records!” will be displayed. pH METER GLP INFORMATION The pH meter GLP information refers to the pH calibration date. To view this information press MENU key while in pH meter mode then GLP. A set of information regarding electrode calibration is displayed.
TEMPERATURE CALIBRATION PROCEDURE (for technical personnel only) All the instruments are factory calibrated for temperature. HANNA’s temperature probes are interchangeable and no temperature calibration is needed when they are replaced. If the temperature measurements are inaccurate, temperature recalibration should be performed. For an accurate recalibration, contact your dealer or the nearest HANNA Customer Service Center, or follow the instructions bellow.
• Immerse the temperature probe in the second vessel as near as possible to the reference thermometer. Allow a few seconds for the probe to stabilize. • Use the ARROW keys to set the calibration point value to that of the hot water, measured by the reference thermometer. • The (unstable measurement) symbol will be shown on the display until the reading becomes stable.
TROUBLESHOOTING GUIDE SYMPTO MS PRO BLEM SO LU TI O N Slow resp on se/excessive d rift. D irty p H or referen ce electrod e. Soa k th e electrod e tip HI 7061 or HI 8061 solu tion for 30 min u tes a n d follow th e clea n in g p roced u re. Pop th e referen ce electrod e a n d refill with electrolyte. Rea d in g flu ctu a tes u p a n d d own (n oise). Clog g ed /d irty ju n ction . L ow electrolyte level (refilla b le p H electrod es on ly). Clea n th e electrod es a n d th e referen ce.
SYMPTO MS PRO BLEM SO LU TI O N After a titra tion in High Range th e in stru m en t d isp la ys 75°SH or 200°TH or 75°D or 2.0%I.a . (a ccord in g with th e selected u n it) b lin kin g . Broken electrod es. In stru m en t n ot ca lib ra ted . Wron g sa m p le. Con cen tra tion ou t of ra n g e. Ch eck/clea n th e electrod es. Reca lib ra te th e in stru m en t (p u m p a n d p H). Ta ke ca re a t sa m p le p rep a ra tion . At sta rtu p th e m eter d isp la ys th e HANNA log o p erm a n en tly.
REFERENCE ELECTRODE CLEANING • Drain the old filling solution, rinse with an adequate HI 7072 solution, drain, then refill with HI 7072 solution. • Do not use an electrode if crystalized salts are visible inside the electrode. Drain electrode, dissasamble and rinse internal body with deionized water. Reassemble and refill with fresh fill solution. • The internal chamber of this electrode is gell filled.
WARRANTY HI 84429 is warranted for two years against defects in workmanship and materials when used for its intended purpose and maintained according to the instructions. This warranty is limited to repair or replacement free of charge. Damage due to accident, misuse, tampering or lack of prescribed maintenance is not covered. If service is required, contact your dealer. If under warranty, report the model number, date of purchase, serial number and the nature of the failure.
Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA Technical Support for Customers Tel. (800) 426 6287 Fax (401) 765 7575 E-mail tech@hannainst.com www.hannainst.
