Instruction Manual HI 84102 TITRATABLE TOTAL ACIDITY MINITITRATOR for wine analysis w w w. h a n n a i n s t .
Dear Customer, Thank you for choosing a Hanna product. This manual will provide you with the necessary information for the correct use of the instrument. Please read it carefully before using the meter. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com. This instrument is in compliance with directives. TABLE OF CONTENTS PRELIMINARY EXAMINATION .................................................................................................
PRELIMINARY EXAMINATION Please examine this product carefully. Make sure that the instrument is not damaged. If any damage occured during shipment, please notify your Dealer.
By simply pressing the START STOP button, the instrument will automatically make the titration up to the end point. The result is immediately displayed in convenient units, then the instrument is ready for another titration. SIGNIFICANCE OF USE Acids occur naturally during the growing of grapes and as part of the fermentation process. Wines show lower levels of acid when there are hot growing seasons or when the grapes come from hotter regions.
SPECIFICATIONS Range Resolution Accuracy Method Principle pH Calibration Sample volume Temperature Compensation pH Electrode Temperature Probe Pump debit Stirring speed Environment Power supply Dimensions Weight REQUIRED REAGENTS Code HI 84102-50 HI 84102-55 HI 84102-60 HI 84100-61 0.0 to 25.0 g/L of tartaric acid 0.1 g/L 5% of reading Acid-base titration method End-point titration One-point in selected end-point: 7.00 pH or 8.20 pH 2 mL Automatic from 0.0 to 100.
PRINCIPLE OF OPERATION The determination of total acids in wine is made according to a neutralization reaction, that is the reaction between the acids found in wine and a base. This type of reaction forms the basis of titration methods of analysing acids. Titratable acidity is measured on a degassed sample at the end-point of 8.20 for Australian requirements and 7.00 to fulfil the requirements of the European Union. Both results are expressed as g/L tartaric acid.
FUNCTIONAL DESCRIPTION INSTRUMENT DESCRIPTION FRONT PANEL 1) 2) 3) 4) 5) Titrant bottle Liquid Crystal Display (LCD) Keypad Electrode holder Peristaltic pump tube REAR PANEL 7
6) 7) 8) 9) 10) 11) 12) 13) 14) pH Electrode Temperature probe Beaker Temperature probe socket BNC electrode connector Fuse Power switch Power cable connector Peristaltic pump KEYPAD DESCRIPTION 1) 2) 3) 4) 5) 6) PURGE - to start/stop purging (max purging time is 5 min) CAL PUMP - to enter pump calibration mode START STOP - to start/stop titration, pump calibration or pH calibration SETUP - to enter/exit SETUP menu STIR - to start/stop the stirrer while in measurement, pH calibration or purging mode CAL
1) 2) 3) 4) 5) 6) 7) 8) 9) 10) 11) 12) Selected mode: SETUP, CAL or CFM for confirming different values Stability indicator: when reading is unstable or calibration is in progress Stirrer active tags Calibration messages Pump active tags Calibration messages “pH” tag: when a buffer is displayed on the secondary display Four digit and half secondary display “Time” tag: when the time is displayed on the secondary display Four digit and half main display “pH” upper tag: when performing a pH calibration or ent
GUIDE TO DISPLAY CODES This prompt appears for a few seconds each time the instrument is turned ON. Main screen display. Main screen display with stirrer active. Purging mode message. PUMP CALIBRATION MESSAGES This screen appears each time the meter enters pump calibration mode. The meter is ready to start pump calibration by pressing the START STOP button. This screen appears while pump calibration is in progress. Pressing CAL PUMP or START STOP button, the minititrator returns to the main screen.
This error message appears when the standard solution is wrong. This error message appears when the input readings (pH, temperature) exceed the input limits. pH CALIBRATION MESSAGES This screen appears each time the titrator enters pH calibration mode. This screen appears when the pH calibration is started by pressing the START STOP button. Pressing again START STOP or the CAL button, the pH calibration is aborted and the instrument returns to the main screen.
TITRATION MESSAGES This screen appears each time the minititrator enters TITRATION mode. Press the START STOP button in order to stop the titration and return to the main screen. The titration result, expressed as concentration of tartaric acid in g/L, is displayed at the end of the titration process. Press the START STOP button to return to the main screen. This error message appears when the input reading (pH, temperature) exceeds the input limits.
MEASUREMENT PROCEDURE Warning: Make sure the instrument has been calibrated (pH and pump calibration) before performing a wine sample analysis. • Use the 2000 µL automatic pipette to add exactly 2 mL of wine sample to the 50 mL beaker. • Fill the beaker up to the 50 mL mark with deionized water, place the stirrer bar into the beaker and then place the beaker in the appropriate place on the minititrator top. • Place the electrode holder on the top of the beaker and secure it by turning clockwise.
pH CALIBRATION PROCEDURE The pH calibration must be performed each time the pH electrode is changed. • Turn the instrument ON using the power switch from the rear panel of the instrument. • Fill the 50 mL beaker up to the 50 mL mark with Buffer Solution 1 (7.00 pH) or Buffer Solution 2 (8.20 pH). • Place the pH electrode into solution and press the CAL button. The CAL message will appear blinking along with pH value. • Press START STOP in order to start the electrode calibration.
• Press START STOP in order to start the pump calibration. • At the end of the calibration procedure done appears for a few seconds and then the meter automatically returns to measurement mode. PUMP TUBE REPLACEMENT To remove the tube of the peristaltic pump follow next steps: • Detach the old tube system from the reagent bottle. • Grasp one fixing ring of the peristaltic pump tube. • Pull the tube until it’s taken out from its location. • Remove the other side of the tube.
FUSE REPLACEMENT To change the fuse follow next steps: • Disconnect the power cord from the rear panel of the instrument. • Pull out the fuse holder located near the power cord connector. • Replace the fuse with a similar one. • Push the fuse holder with the fuse in the appropriate place. ELECTRODE CONDITIONING & MAINTENANCE PREPARATION PROCEDURE Remove the protective cap of the pH electrode (HI 1048B). DO NOT BE ALARMED IF SALT DEPOSITS ARE PRESENT. This is normal with electrodes.
If the filling solution (electrolyte) is more than 2½ cm (1”) below the fill hole, add HI 7082 3.5M KCl Electrolyte Solution. For faster response, unscrew the fill hole screw during measurements. STORAGE PROCEDURE To minimize clogging and assure a quick response time, the glass bulb and the junction of the electrode should be kept moist and not allowed to dry out. Replace the solution in the protective cap with a few drops of HI 70300 Storage Solution or, in its absence, Filling Solution (HI 7082).
ACCESSORIES REAGENT SETS HI 70300LElectrode storage solution (500mL) HI 70635LCleaning solution for wine deposits (500mL) HI 70636LCleaning solution for wine stains (500 mL) HI 7082 Electrode filling solution (4 X 30 mL) HI 84102-50 Titrant solution (110mL) HI 84102-55 Calibration standard (100mL) HI 84102-60 Buffer solution 1 pH 7.00 (500 mL) HI 84102-61 Buffer solution 2 pH 8.
WARRANTY HI 84102 is warranted for two years against defects in workmanship and materials when used for its intended purpose and maintained according to the instructions. This warranty is limited to repair or replacement free of charge. Damage due to accident, misuse, tampering or lack of prescribed maintenance is not covered. If service is required, contact your dealer. If under warranty, report the model number, date of purchase, serial number and the nature of the failure.
SALES AND TECHNICAL SERVICE CONTACTS Australia: Tel. (03) 9769.0666 • Fax (03) 9769.0699 China: Tel. (10) 88570068 • Fax (10) 88570060 Egypt: Tel. & Fax (02) 2758.683 Germany: Tel. (07851) 9129-0 • Fax (07851) 9129-99 Greece: Tel. (210) 823.5192 • Fax (210) 884.0210 Indonesia: Tel. (21) 4584.2941 • Fax (21) 4584.2942 Japan: Tel. (03) 3258.9565 • Fax (03) 3258.9567 Korea: Tel. (02) 2278.5147 • Fax (02) 2264.1729 Malaysia: Tel. (603) 5638.9940 • Fax (603) 5638.9829 Singapore: Tel. 6296.
