SALES AND TECHNICAL SERVICE CONTACTS Instruction Manual Australia: Tel. (03) 9769.0666 • Fax (03) 9769.0699 HI 83749 China: Tel. (10) 88570068 • Fax (10) 88570060 Turbidity & Bentocheck Egypt: Tel. & Fax (02) 2758.683 Germany: Tel. (07851) 9129-0 • Fax (07851) 9129-99 Greece: Tel. (210) 823.5192 • Fax (210) 884.0210 Indonesia: Tel. (21) 4584.2941 • Fax (21) 4584.2942 Japan: Tel. (03) 3258.9565 • Fax (03) 3258.9567 Korea: Tel. (02) 2278.5147 • Fax (02) 2264.1729 Malaysia: Tel.
Dear Customer, Thank you for choosing a Hanna Instruments product. This manual will provide you with the necessary information for correct use of the instrument. Please read this instruction manual carefully before using the instrument. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com or see the back side of this manual for our worldwide sales and technical service contacts. This instrument is in compliance with directives. TABLE OF CONTENTS WARRANTY ........
PC INTERFACE PRELIMINARY EXAMINATION To fully use the instrument tag identification system function, the measured data has to be downloaded to a computer. The instrument can use RS232 or USB connection to communicate with the PC. When using the RS232 protocol, simply connect a HI 920011 serial cable between the instrument and the computer. To use the USB protocol, simply connect a regular USB cable between instrument and PC.
GENERAL DESCRIPTION GENERAL DESCRIPTION The HI 83749 is an auto diagnostic microprocessor meter that benefits from Hanna’s years of experience as manufacturer of analytical instruments. The meter is especially designed for wine analyses and, beside turbidity measurements, it allows to make tests to verify protein stability (e.g. Bentocheck). The instrument compensates wine color to guarantee accurate readings during the vinification process; also for the darkest red wine samples.
BATTERIES MANAGEMENT For field measurements, HI 83749 is powered by 4 AA batteries. The battery life is enough for 1500 normal measurements. When you turn the instrument on, the remaining battery life is estimated and reported in percents. To save the battery life, the instrument will turn off after 15 minutes of non-use. The backlight will be turn off after 25 seconds since the last key was pressed.
PRINCIPLE OF OPERATION A light beam that passes through the sample is scattered in all directions. The intensity and pattern of the scattered light is affected by many variables like wavelenght of the incident light, particle size, shape, refractive index and color. The optical system includes a tungsten filament lamp, a scattered light detector (90 0) and a transmitted light detector (1800). The microprocessor of the instrument calculates the NTU value from the signals that reaches the two detectors.
SPECIFICATIONS SET INSTRUMENT ID The instrument ID is a four digit number that can be edited by the user. The instrument ID is downloaded on the PC application, together with the logged data. By setting a different ID for each instrument it is possible to mix information from many turbidimeters into the same database. • To set the instrument ID, press CAL/RCL when the “Set instrument ID” panel is displayed. The default instrument ID is 0000. The existing ID value and the CFM tag will start blinking.
FUNCTIONAL DESCRIPTION • Press LOG/CFM or READ to start editing the day value. The day value will start blinking. INSTRUMENT DESCRIPTION • Press the UP or DOWN keys to set the day value. Note: to edit the year again, after the day was set, press READ . or • Press LOG/CFM to save the new date. The new date will be displayed on the LCD. Alternatively, press CAL/RCL to exit without saving the changes. SET THE TIME 1) 2) 3) 4) Cuvet Lid. Cuvet Holder. Backlit Liquid Crystal Display (LCD).
SHOW / HIDE THE TIME You can choose between showing or hiding the current hour and minutes on the secondary LCD. • To set hiding or showing the time, press CAL/RCL when the “Show/hide time” panel is displayed. The time show status and the CFM tag will start blinking. • Press the UP or DOWN keys to set Lcd / hide for time. 11) CAL/RCL: this is a bi-functional key. Just press to enter/exit calibration or in setup mode to start/stop editing a parameter.
GENERAL TIPS FOR AN ACCURATE MEASUREMENT The instructions listed below should be carefully followed to ensure best accuracy. GENERAL TIPS • • • • Always cap the cuvets to avoid spillage of the sample into the instrument. Always close the lid of the instrument during measurement. Keep the lid of the instrument closed when it is not used to prevent dust or dirt entering. Put always the instrument on a flat, rugged surface when taking measurements.
REMOVING AIR BUBBLES • Add HI 83749-0 Bentocheck reagent, mix and wait for 1 minute. Then take a new reading (T2). • If the difference between T1 and T2 is less than 10%, the instrument gives a beep of 1 second and displays alternating the turbidity value (NTU) and the difference (%). • If the difference between T1 and T2 is more than 10%, the instrument just displays T2 in the primary LCD and T1 in the secondary LCD.
CALIBRATION PROCEDURE SETUP It is recommended to calibrate the meter only with Hanna ready-to-use calibration standard solutions. Alternatively, formazin standards can be used. The prepared formazin solutions should be close to the default calibration points. The first point must be near 0 NTU, the second point between 10 and 20 NTU, the third point between 50 and 150 NTU and the fourth point between 400 and 600 NTU. The Setup mode allows the user to view and modify the instrument parameters.
• Remove the <0,10 NTU standard cuvet and place the 15.0 NTU standard cuvet into the holder. • Third calibration point (if available). • Fourth calibration point (if available). • Close the lid and press READ . “----” and the “Lamp and Read Status” indicator will blink again while making the reading. • Delete calibration panel. • At the end of the reading, the third calibration point (100 NTU) and “CAL P.3” is displayed. If desired, the value can be changed using the UP or DOWN keys.
• Remove the 100 NTU standard cuvet from the meter and insert the 500 NTU standard cuvet into the holder. • The instrument asks for confirmation. Press the LOG/CFM key to confirm all records are deleted. To abort the delete function, press READ instead of LOG/CFM. • After all records are deleted the instrument returns to measurement mode. • Close the lid and press READ . “----” and the “Lamp and Read Status” indicator will blink again while making the reading.
DELETE CALIBRATION HI 83749 is factory calibrated. It is possible to restore factory calibration by deleting last performed calibration. To delete last calibration, follow the next steps: • Enter the GLP feature by pressing GLP . The date of the last calibration will be displayed on the LCD (e.g. 2005.02.23). • Measurement date in YYYY.MM.DD format. • Measurement time in hh:mm format. • Delete the last record panel (only for last record). • Delete all records.
TURBIDITY MEASUREMENT VIEW LOGGED DATA Note: For wine analysis it is recommended to work always with the AVG mode on. • Turn the instrument ON by pressing ON/OFF. • When the LCD displays “----”, the instrument is ready. The current time appears on the secondary LCD, if selected in Setup menu. • Fill a clean, dry cuvet with 10 mL of wine up to the mark, taking care to handle the cuvet by the top. Replace the cap. Note: To remove any fingerprints or dirt, wipe the cuvet thoroughly with a lint-free cloth.
LOGGING AVG (SIGNAL AVERAGE MODE) HI 83749 has a log space for up to 200 records. With each measurement, the date, time and tag ID is stored. In this way, each record is fully characterized and can be easily analyzed when downloading data on the PC application (HI 92000). LOGGING The log function is active after a valid measurement is obtained (no errors). • To log a value, press LOG/CFM when the measurement result is displayed.
BENTOCHECK (PROTEIN STABILITY TEST) • Turn the instrument ON by pressing ON/OFF. When the LCD displays “----”, the instrument is ready. Note: On the secondary LCD the current time appears, if selected in Setup menu. • To define the g/hL of bentonite to be added to the wine tank, just multiply by 100 the mL of 2,5% bentonite suspension that was added to the HANNA vial (0.25 mL for vial #1, 0.50 mL for #2, 0.75 mL for #3 and 1.
• Place the cuvet #1 into the instrument and close the lid. • Replace the cap. Invert several times to mix and then wait for 1 minute. • Press READ and “----” will blink on the display. After a few seconds the instrument displays the first reading. The meter continues updating the readings until the “Lamp and Read Status” indicator turns off. • The final displayed value is the averaged reading. This is T1 for sample #1. Record the value. • Remove cuvet #1 from the instrument and open the cap.
• Wait for 15 minutes to allow suspended matter to settle. DETERMINATION OF BENTONITE REQUIREMENT SAMPLE PREPARATION • Fill 4 HANNA vials with 25 mL of unfiltered wine sample. • Fold a filter disc twice as shown in the figure. • Prepare a bentonite suspension of 2.5%. Note: use always a bentonite suspension with the same wetting degree as the suspension that is used in production. • Use the 1 mL syringe to add 0.25 mL of bentonite suspension to the vial #1; then add 0.50 mL to #2, 0.
