Instruction Manual HI 83742 COLOR & PHENOLS ISM for wine analysis MAN83742R3 04/06 w w w . h a n n a i n s t . c o m www.hannainst.
Dear Customer, Thank you for choosing a Hanna product. This manual will provide you with the necessary information for the correct use of the instrument. Please read it carefully before using the meter. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com. This instrument is in compliance with directives. TABLE OF CONTENTS PRELIMINARY EXAMINATION ...............................................................................................
ACCESSORIES PRELIMINARY EXAMINATION REAGENT SETS HI 83742-20 Phenols reagent set for wine (20 tests) HI 83742-25 Color reagent set for wine (20 tests) containing HI 83742-0 Wine Solvent 1 HI 83742-27 Color reagent set for wine (20 tests) containing HI 83742-3 Wine Solvent 3 OTHER ACCESSORIES DEMI-10 Bottle to prepare 10 liters of demineralized water HI 740027P 1.
GENERAL DESCRIPTION MEASUREMENT The HI 83742 is an auto-diagnostic portable microprocessor meter that benefits from Hanna’s years of experience as a manufacturer of analytical instruments. It has an advanced optical system based on a special tungsten lamp and a narrow band interference filter that allows most accurate and repeatable readings. All instruments are factory calibrated. The auto-diagnostic feature of this meter ensures always optimal measurement conditions to ensure most precise readings.
• Use the 200 µL automatic pipette with a clean pipette tip to add exactly 0,2 mL of diluted red wine sample. • Add 6 drops of HI 83742B-0 Folin & Ciocalteu’s Reagent to the cuvet. x6 The reaction between phenols and the Folin-Ciocalteu reagent involves oxidation of the phenolic groups (ROH) with a mixture of phosphotungstenic acid (H3PW12O40) and phosphomolybdenic acid (H3PMo12O40) to the quinoid form (R=O).
PRINCIPLE OF OPERATION TOTAL PHENOLS, RED WINE Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules or crystal lattices.
MEASUREMENT • Press “RANGE” to select the parameter code “P4” for total phenols, white wine (see page 13). #1 (Zero) • Fill an empty cuvet with deionized water and replace the cap. This is the zero zero. • Insert the zero cuvet into the holder and close the lid. • Press ZERO and “----” will blink on the display. • After a few seconds the display will show “-0.0-”. The meter is now zeroed and ready for measurement.
FUNCTIONAL DESCRIPTION • Add 6 drops of HI 83742B-0 Folin & Ciocalteu’s Reagent to the cuvet. INSTRUMENT DESCRIPTION x6 1) 2) 3) 4) 5) 6) 7) 8) FRONT Lid Cuvet Holder Liquid Crystal Display (LCD) ON/OFF key, to turn the meter on and off ZERO key, to zero the meter RANGE key, to select parameter READ/TIMER key, to perform measurement Power Socket 12V to 20V DC 2.5 Watt • Replace the cap and shake gently to mix.
TOTAL PHENOLS, WHITE WINE SPECIFICATIONS Range Resolution Precision Light Source Method GUIDE TO DISPLAY CODES 0.000 to 0.750 g/L 0.001 ±0.015 @ 0.350 g/L Tungsten lamp with narrow band interference filter @ 610 nm The reaction between Phenols and the reagents causes a blue tint in the sample. REQUIRED REAGENTS Code Description HI 83742A-0 Acid Reagent HI 83742B-0 Folin & Ciocalteu’s Reagent HI 83742C-0 Carbonate Buffer Quantity/test 5 mL 6 drops 4.
Indicates that the batteries are dead and must be replaced. After this message appears, the instrument is switched off. Change the batteries and restart the meter. #1 (Zero) • Fill an empty cuvet with deionized water and replace the cap. This is the zero zero. ERROR MESSAGES The meter has lost its configuration. Contact your dealer or the nearest Hanna Customer Service Center. a) on zero reading: • Insert the zero cuvet into the holder and close the lid.
COLOR HUE GENERAL TIPS FOR AN ACCURATE MEASUREMENT SPECIFICATIONS Range Resolution Precision Light Source Method The instructions listed below should be carefully followed during testing to ensure best accuracy. 0.00 to 9.99 0.01 ±0.03 @ 0.75 Tungsten lamp with narrow band interference filters @ 420 and 520 nm Direct reading. REQUIRED REAGENTS Code Description HI 83742-0 Wine Solvent-1 Quantity/test 8 mL For reagent set and other accessories see page 26.
• Proper use of the dropper: (a) to get good reproducible results, tap the dropper on the table for several times and wipe the outside of the dropper tip with a cloth. (b) always keep the dropper bottle in a vertical position while dosing the reagent. MEASUREMENT • Press “RANGE” to select the parameter code “P2” for color density - red wine (see page 13). #1 (Zero) • Fill an empty cuvet with deionized water and replace the cap. This is the zero zero.
COLOR DENSITY, RED WINE OPERATIONAL GUIDE SPECIFICATIONS Range Resolution Precision Light Source Method MEASUREMENT PROCEDURE 0.00 to 15.00 0.01 ±0.20 @ 5.00 Tungsten lamp with narrow band interference filters @ 420 and 520 nm Direct reading. REQUIRED REAGENTS Code Description HI 83742-0 Wine Solvent-1 HI 83742-3 Wine Solvent-3 For reagent set and other accessories see page 26. • The meter will first perform an LCD self diagnostic test by displaying a full set of figures.
COLOR DENSITY, WHITE WINE SPECIFICATIONS Range Resolution Precision Light Source Method • After a few seconds the display will show “-0.0-”. The meter is now zeroed and ready for measurement. 0.000 to 1.000 0.001 ±0.010 @ 0.200 Tungsten lamp with narrow band interference filter @ 420 nm Direct reading. • Remove the cuvet from the instrument. #2 (Sample) REQUIRED REAGENTS No reagents required. For other accessories see page 26. • Insert the sample (cuvet #2) into the holder and close the lid.
