Instruction Manual HI 83225 Grow Master for Nutrient Analyses www.hannainst.
Dear Customer, Thank you for choosing a Hanna product. Please read this instruction manual carefully before using the instrument. This manual will provide you with the necessary information for the correct use of the instrument. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com. TABLE OF CONTENTS PRELIMINARY EXAMINATION ..............................................................................................................................................
PRELIMINARY EXAMINATION Please examine this product carefully. Make sure that the instrument is not damaged. If any damage occurred during shipment, please notify your local Hanna Office.
ABBREVIATIONS °C: °F: μg/L: mg/L: g/L: mL: HR: MR: LR: degree Celsius degree Fahrenheit micrograms per liter (ppb) milligrams per liter (ppm) grams per liter (ppt) milliliter high range medium range low range SPECIFICATIONS Life of the instrument Silicon Photocell 0 to 50°C (32 to 122°F); max 95% RH non-condensing Power Supply external 12 Vdc power adapter built-in rechargeable battery Dimensions 235 x 200 x 110 mm (9.2 x 7.87 x 4.33") Weight 0.9 Kg For specifications related to each method (e.g.
PRINCIPLE OF OPERATION Absorption of light is a typical phenomenon of interaction between electromagnetic radiation and matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules or crystal lattices.
The measurement process is carried out in two phases: first the meter is zeroed and then the actual measurement is performed. The cuvette has a very important role because it is an optical element and thus requires particular attention. It is important that both the measurement and the calibration (zeroing) cuvette are optically identical to provide the same measurement conditions. Most methods use the same cuvette for both, so it is important that measurements are taken at the same optical point.
1) 2) 3) 4) 5) 6) 7) 8) Open Cuvette Lid Indexing mark Cuvette point Liquid Crystal Display (LCD) Splash proof keypad ON/OFF power switch Power input connector USB connector KEYPAD DESCRIPTION The keypad contains 8 direct keys and 3 functional keys with the following functions: Press to perform the function displayed above it on the LCD. ESC Press to exit the current screen. Press to access the select method menu.
TIPS FOR AN ACCURATE MEASUREMENT The instructions listed below should be carefully followed during testing to ensure most accurate results. • Color or suspended matter in large amounts may cause interference, and should be removed by treatment with active carbon and filtration: refere to SAMPLE PREPARATION Chapter (see page 20). • Ensure the cuvette is filled correctly: the liquid in the cuvette forms a convexity on the top; the bottom of this convexity must be at the same level as the 10 mL mark.
• Proper use of the powder reagent packet: (a) use scissors to open the powder packet; (b) push the edges of the packet to form a spout; (c) pour out the content of the packet. USING CUVETTES • Proper mixing is very important for reproducibility of the measurements. The right way of mixing a cuvette is specified for each method in the related chapter. (a) invert the cuvette a couple of times or for a specified time: hold the cuvette in the vertical position with the cap up.
• Pay attention to push the cuvette completely down in the holder and to align the white point on the cap to the indexing mark on the meter. • In order to avoid reagent leaking and to obtain more accurate measurements, close the cuvette first with the supplied HDPE plastic stopper and then the black cap. • Each time the cuvette is used, the cap must be tightened to the same degree. • Whenever the cuvette is placed into the measurement cell, it must be dry outside, and free of fingerprints, oil or dirt.
HEALTH & SAFETY • The chemicals contained in the reagent kits may be hazardous if improperly handled. • Read the Material Safety Data Sheet (MSDS) before performing tests. • Safety equipment: Wear suitable eye protection and clothing when required, and follow instructions carefully. • Reagent spills: If a reagent spill occurs, wipe up immediately and rinse with plenty of water. If reagent contacts skin, rinse the affected area thoroughly with water. Avoid breathing released vapors.
OPERATIONAL GUIDE POWER CONNECTION AND BATTERY MANAGEMENT The meter can be powered from an AC/DC adapter (included) or from the built-in rechargeable battery. Note: Always turn the meter off before unplugging it to ensure no data is lost. When the meter switches ON, it verifies if the power supply adapter is connected.
• After the desired method is selected, follow the measurement described in the related section. • Before performing a test you should read all the instructions carefully. DATA MANAGEMENT The instrument features a data log function to help you keep track of all your analysis. The data log can hold 200 individual measurements. Storing, viewing and deleting the data is possible using the LOG and RCL keys.. Storing data: You can store only a valid measurement.
SETUP In the Setup mode the instrument’s parameters can be changed. Some parameters affect the measuring sequence and others are general parameters that change the behavior or appearance of the instrument. Press SETUP to enter the setup mode. Press ESC or SETUP to return to the main screen. A list of setup parameters will be displayed with currently configured settings. Press HELP for additional information. Press the keys to select a parameter and change the value as follows: Backlight Values: 0 to 8.
Date / Time This option is used to set the instrument’s date and time. Press the Modify key to change the date/time. Press the W X functional keys to highlight the value to be modified (year, month, day, hour, minute or second). Use the keys to change the value. Press the Accept key to confirm or ESC to return to the setup without saving the new date or time. Time format Option: AM/PM or 24 hour. Press the functional key to select the desired time format.
Instrument ID Option: 0 to 9999. This option is used to set the instrument’s ID (identification number). The instrument ID is used while exchanging data with a PC. Press the Modify key to access the instrument ID screen. Press the keys in order to set the desired value. Press the Accept key to confirm the value or ESC to return to the setup menu without saving the new value. Meter information Press the Select key to view the instrument model, firmware version, language version and instrument serial number.
INTRODUCTION THE ROLE OF NUTRIENTS IN THE GROWTH AND PRODUCTION OF PLANTS The three elements that are mostly needed by the plants are nitrogen (N), phosphorus (P) and potassium (K). They are called the macronutrients while other elements, needed by plants in smaller amounts, are called microelements. In hydroponics, plants need a balanced nutritive solution, composed of macro and microelements.
PHOSPHORUS Phosphorus (P) has an important role in many fundamental biochemical and physiological processes. Plants take up phosphorus in the form of phosphate ion (PO43¯).
IRRIGATION WATER (LOW RANGE) In agricultural areas it is quite common to find altered values in the chemical composition of irrigation waters. The problem concerns mostly the high nitrate concentration, usually determined by excessive fertilization or irrational liquid manure spreading. The analysis of irrigation waters allows us to find out which are the substances present in major or minor quantity and to organize an advantageous fertilization plan.
SAMPLE PREPARATION SAMPLE PREPARATION PROCEDURE Note: If the water sample to be analyzed is very turbid, let it stand in a beaker for a while until most of the solid particles have settled. Then, use the pipette to transfer the supernatant solution to the other beaker and prepare the samples as described below. To prevent the displacement of the settled solids at the bottom of the beaker, do not induce air bubbles into the solution.
• Fold a filter disc twice as shown in the figure. Separate one side from the other three to form a cone. Insert the folded filter disc in the funnel. • Filter the treated sample into an empty beaker. The sample is now ready. Note: Filter at least 40 mL of solution if all the four methods will be tested. If the solution is still turbid or colored, treat it again with a packet of active carbon. After use, throw the filter disc away and wash the syringe and the filter assembly well.
• Replace the cap and shake gently for at least 2 minutes. • Open the upper part of the Demineralizer Bottle cap and gently squirt the demineralized water into the cylinder, up to the 100 mL mark. Note: The ion exchange resin contained in the Demineralizer Bottle is provided with an indicator substance. The indicator will change from green to blue when the resin has been exhausted and needs to be replaced. • Pour the solution in the large 170 mL beaker, replace the cap and invert several times to mix.
Note: To measure exactly 5 mL of sample with the syringe, push the plunger completely into the syringe and insert the tip into the sample. Pull the plunger out until the lower edge of the seal is on the 5 mL mark of the syringe. • Remove the cap and fill the Demineralizer Bottle with tap water. • Replace the cap and shake gently for at least 2 minutes. • Open the upper part of the Demineralizer Bottle cap and squirt gently the demineralized water into the cylinder, up to the 100 mL mark.
AMMONIA HIGH RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Dev. Light Source Method 0 to 100 mg/L 1 mg/L ±1 mg/L ±4% of reading at 25 °C ±1 mg/L Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92, Nessler method. The reaction between ammonia and reagents causes a yellow tint in the sample.
• Remove the cuvette. • Add 4 drops of HI 93715A-0 First Reagent (6 drops for seawater analysis). Replace the cap and mix the solution by inverting the cuvette a couple of times. • Add 4 drops of HI 93715B-0 Second Reagent (10 drops for seawater analysis). Replace the cap and mix the solution by inverting the cuvette a couple of times. • Reinsert the cuvette into the instrument.
AMMONIA MEDIUM RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Dev. Light Source Method 0.0 to 50.0 mg/L 0.5 mg/L ±0.5 mg/L ±4% of reading at 25 °C ±0.5 mg/L Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92, Nessler method. The reaction between ammonia and reagents causes a yellow tint in the sample.
• Remove the cuvette. • Add 4 drops of HI 93715A-0 First Reagent (6 drops for seawater analysis). Replace the cap and mix the solution by inverting the cuvette a couple of times. • Add 4 drops of HI 93715B-0 Second Reagent (10 drops for seawater analysis). Replace the cap and mix the solution by inverting the cuvette a couple of times. • Reinsert the cuvette into the instrument.
AMMONIA LOW RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Dev. Light Source Method 0.0 to 10.0 mg/L 0.01 mg/L ±0.1 mg/L ±4% of reading at 25 °C ±0.1 mg/L Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92, Nessler method. The reaction between ammonia and reagents causes a yellow tint in the sample.
• Remove the cuvette. • Add 4 drops of HI 93715A-0 First Reagent (6 drops for seawater analysis). Replace the cap and mix the solution by inverting the cuvette a couple of times. • Add 4 drops of HI 93715B-0 Second Reagent (10 drops for seawater analysis). Replace the cap and mix the solution by inverting the cuvette a couple of times. • Reinsert the cuvette into the instrument.
NITRATE HIGH RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Dev. Light Source Method 0 to 300 mg/L 5 mg/L ±10 mg/L ±8% of reading at 25 °C ±5 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the cadmium reduction method. The reaction between nitrate-nitrogen and the reagent causes an amber tint in the sample.
• Replace the cap and immediately shake vigorously up and down for exactly 10 seconds. Continue to mix by inverting the cuvette gently for 50 seconds, while taking care not to induce air bubbles. Powder will not completely dissolve. Time and way of shaking could sensitively affect the measurement. • Reinsert the cuvette into the instrument, taking care not to shake it.
NITRATE MEDIUM RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Dev. Light Source Method 0 to 150 mg/L 2.5 mg/L ±5 mg/L ±8% of reading at 25 °C ±2.5 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the cadmium reduction method. The reaction between nitrate-nitrogen and the reagent causes an amber tint in the sample.
• Replace the cap and immediately shake vigorously up and down for exactly 10 seconds. Continue to mix by inverting the cuvette gently for 50 seconds, while taking care not to induce air bubbles. Powder will not completely dissolve. Time and way of shaking could sensitively affect the measurement. • Reinsert the cuvette into the instrument, taking care not to shake it.
NITRATE LOW RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Dev. Light Source Method 0.0 to 30.0 mg/L 0.5 mg/L ±1.0 mg/L ±8% of reading at 25 °C ±0.5 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the cadmium reduction method. The reaction between nitrate-nitrogen and the reagent causes an amber tint in the sample.
• Replace the cap and immediately shake vigorously up and down for exactly 10 seconds. Continue to mix by inverting the cuvette gently for 50 seconds, while taking care not to induce air bubbles. Powder will not completely dissolve. Time and way of shaking could sensitively affect the measurement. • Reinsert the cuvette into the instrument, taking care not to shake it.
PHOSPHORUS HIGH RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Dev. Light Source Method 0 to 100 mg/L 1 mg/L ±5 mg/L ±4% of reading at 25 °C ±1 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Amino Acid method. The reaction between phosphorus and reagents causes a blue tint in the sample.
• Add the content of one packet of HI 93706B-0 Phosphorus Reagent B (Amino Acid) to the cuvette. Replace the cap and shake gently until completely dissolved. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 5 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of phosphorus (P). • Press or to access the second level functions.
PHOSPHORUS MEDIUM RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Dev. Light Source Method 0.0 to 50.0 mg/L 0.5 mg/L ±2.5 mg/L ±4% of reading at 25 °C ±0.5 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Amino Acid method. The reaction between phosphate and reagents causes a blue tint in the sample.
• Add the content of one packet of HI 93706B-0 Phosphorus Reagent B (Amino Acid) to the cuvette. Replace the cap and shake gently until completely dissolved. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 5 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of phosphorus (P). • Press or to access the second level functions.
PHOSPHORUS LOW RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Dev. Light Source Method 0.0 to 10.0 mg/L 0.1 mg/L ±0.5 mg/L ±4% of reading at 25 °C ±0.1 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Amino Acid method. The reaction between phosphate and reagents causes a blue tint in the sample.
• Add the content of one packet of HI 93706B-0 Phosphorus Reagent B (Amino Acid) to the cuvette. Replace the cap and shake gently until completely dissolved. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 5 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of phosphorus (P). • Press or to access the second level functions.
POTASSIUM HIGH RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Dev. Light Source Method 20 to 200 mg/L 5 mg/L ±30 mg/L ±7% of reading at 25 °C ±5 mg/L Tungsten lamp with narrow band interference filter @ 610 nm Adaptation of the Turbidimetric Tetraphenylborate method. The reaction between Potassium and reagents causes turbidity in the sample.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette and add the content of one packet of HI 93750B-0 reagent. Replace the cap and gently mix for one minute by slowly turning the cuvette upside down. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 2 minutes and press Read. When the timer ends the meter will perform the reading.
INTERFERENCES Interferences may be caused by: Ammonium above 10 ppm Calcium above 10000 ppm as CaCO3 Chloride above 12000 ppm Magnesium above 8000 ppm as CaCO3 Sodium above 8000 ppm POTASSIUM ULTRA HIGH RANGE For samples containing more than 200 ppm of Potassium: follow the sample preparation procedure described at page 22 for NUTRIENTS SOLUTION (HR).
POTASSIUM MEDIUM RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Dev. Light Source Method 10 to 100 mg/L 2.5 mg/L ±15 mg/L ±7% of reading at 25 °C ±2.5 mg/L Tungsten lamp with narrow band interference filter @ 610 nm Adaptation of the Turbidimetric Tetraphenylborate method. The reaction between Potassium and reagents causes turbidity in the sample.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette and add the content of one packet of HI 93750B-0 reagent. Replace the cap and gently mix for one minute by slowly turning the cuvette upside down. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 2 minutes and press Read. When the timer ends the meter will perform the reading.
POTASSIUM LOW RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Dev. Light Source Method 0.0 to 20.0 mg/L 0.5 mg/L ±3.0 mg/L ±7% of reading at 25 °C ±0.5 mg/L Tungsten lamp with narrow band interference filter @ 610 nm Adaptation of the Turbidimetric Tetraphenylborate method. The reaction between Potassium and reagents causes turbidity in the sample.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette and add the content of one packet of HI 93750B-0 reagent. Replace the cap and gently mix for one minute by slowly turning the cuvette upside down. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 2 minutes and press Read. When the timer ends the meter will perform the reading.
CALCIUM SPECIFICATIONS 0 to 400 mg/L 10 mg/L ±10 mg/L ±5% of reading at 25 °C Tungsten lamp with narrow band interference filter @ 466 nm Adaptation of the Oxalate method. Range Resolution Accuracy Light Source Method REQUIRED REAGENTS Code HI 93752A-0 Ca HI 93752B-0 Ca Description Buffer Reagent Calcium Buffer Reagent Calcium Oxalate Reagent Quantity 4 drops 7 mL 1 mL REAGENT SETS HI 937521-01 Reagents for 50 tests HI 937521-03 Reagents for 150 tests For other accessories see page 57.
• Replace the cap and invert several times to mix. • Place the cuvette into the holder and close the lid. • Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette. • Using the 1 mL syringe, add exactly 1 mL of the HI 93752B-0 Calcium Oxalate Reagent. Replace the cap and invert the cuvette 10 times to mix (about 15 seconds). • Press Timer or wait for 5 minutes. Then invert again the cuvette 10 times to mix (about 15 seconds).
MAGNESIUM SPECIFICATIONS Range Resolution Accuracy Light Source Method 0 to 150 mg/L 5 mg/L ±5 mg/L ±3% of reading at 25 °C Tungsten lamp with narrow band interference filter @ 466 nm Adaptation of the Calmagite method. REQUIRED REAGENTS Code Description Quantity HI 93752A-0 Mg Magnesium Buffer Reagent 1 mL HI 93752B-0 Mg Magnesium Indicator Reagent 9 mL REAGENT SETS HI 937520-01 Reagents for 50 tests HI 937520-03 Reagents for 150 tests For other accessories see page 57.
• Remove the cuvette. • Using the other 1 mL syringe, add to the cuvette exactly 0.5 mL of sample. Note: Do not mix up the two syringes! 0.5 mL of sample • Replace the cap and invert several times to mix. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 15 seconds and press Read. When the timer ends the meter will perform the reading. • The instrument displays the results in mg/L of Magnesium (Mg).
SULFATE SPECIFICATIONS Range Resolution Accuracy Light Source Method 0 to 100 mg/L 5 mg/L ±5 mg/L ±3% of reading at 25 °C Tungsten lamp with narrow band interference filter @ 466 nm Sulfate is precipitated with barium chloride crystals. Light absorbance of the suspension is measured. REQUIRED REAGENTS Code HI 93751-0 Description Indicator reagent Quantity 1 packet REAGENT SETS HI 93751-01 Reagents for 100 tests HI 93751-03 Reagents for 300 tests For other accessories see page 57.
• Add the content of one packet of HI 93751-0 Indicator reagent. • Replace the cap and invert gently to mix for 1 minute (about 30 inversions). • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 5 minutes and press Read. When the timer ends the meter will perform the reading. • The instrument displays concentration in mg/L of Sulfate (SO42¯).
ERRORS AND WARNINGS The instrument shows clear warning messages when erroneous conditions appear and when measured values are outside the expected range. These messages are described bellow. No Light: The light source is not functioning properly. Light Leak: There is an excess amount of ambient light reaching the detector. Inverted cuvettes: The sample and the zero cuvettes are inverted. Battery Low: The battery capacity is lower than 10%. Light Low: The instrument cannot adjust the light level.
DATA MANAGEMENT The analyzed data can be managed using Hanna’s product HI92000, Windows ® Compatible Software. Windows® is registered Trademark of "Microsoft Co.
STANDARD METHODS Description Range Ammonia HR Ammonia MR Ammonia LR Nitrate HR Nitrate MR Nitrate LR Phosphorus HR Phosphorus MR Phosphorus LR Potassium HR Potassium MR Potassium LR Calcium Magnesium Sulfate 0 to 100 mg/L 0.0 to 50.0 mg/L 0.0 to 10.0 mg/L 0 to 300 mg/L 0 to 150 mg/L 0.0 to 30.0 mg/L 0 to 100 mg/L 0.0 to 50.0 mg/L 0.0 to 10.0 mg/L 20 to 200 mg/L 10 to 100 mg/L 0.0 to 20.
OTHER ACCESSORIES HI 731318 HI 731321 HI 731325W HI 740034 HI 740036 HI 740157 HI 740223 HI 740224 HI 740225 HI 740226 HI 740227 HI 740228 HI 740229 HI 740230 HI 92000 HI 920013 HI 93703-50 HI 93703-54 HI 93703-55 cloth for wiping cuvettes (4 pcs) glass cuvettes (4 pcs) new cap for cuvette (4 pcs) cap for 100 mL beaker (6 pcs) 100 mL plastic beaker (6 pcs) plastic refilling pipette (20 pcs) 170 mL plastic beaker 170 mL plastic beakers (12 pcs) 60 mL graduated syringe 5 mL graduated syringe filter assembly
WARRANTY All Hanna Instruments meters are warranted for two years against defects in workmanship and materials when used for its intended purpose and maintained according to the instructions. This warranty is limited to repair or replacement free of charge. Damages due to accident, misuse, tampering or lack of prescribed maintenance are not covered. If service is required, contact your dealer. If under warranty, report the model number, date of purchase, serial number and the nature of the failure.
Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA Technical Support for Customers Tel. (800) 426 6287 Fax (401) 765 7575 E-mail tech@hannainst.com www.hannainst.
