Instruction Manual HI 83215 Grow Master Basic for Nutrient Analyses www.hannainst.
Dear Customer, Thank you for choosing a Hanna product. Please read this instruction manual carefully before using the instrument. This manual will provide you with the necessary information for the correct use of the instrument. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com. TABLE OF CONTENTS PRELIMINARY EXAMINATION ..............................................................................................................................................
PRELIMINARY EXAMINATION Please examine this product carefully. Make sure that the instrument is not damaged. If any damage occurred during shipment, please notify your local Hanna Office.
ABBREVIATIONS °C: °F: μg/L: mg/L: g/L: mL: HR: MR: LR: degree Celsius degree Fahrenheit micrograms per liter (ppb) milligrams per liter (ppm) grams per liter (ppt) milliliter high range medium range low range SPECIFICATIONS Life of the instrument Silicon Photocell 0 to 50°C (32 to 122°F); max 95% RH non-condensing Power Supply external 12 Vdc power adapter built-in rechargeable battery Dimensions 235 x 200 x 110 mm (9.2 x 7.87 x 4.33") Weight 0.9 Kg For specifications related to each method (e.g.
PRINCIPLE OF OPERATION Absorption of light is a typical phenomenon of interaction between electromagnetic radiation and matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules or crystal lattices.
The measurement process is carried out in two phases: first the meter is zeroed and then the actual measurement is performed. The cuvette has a very important role because it is an optical element and thus requires particular attention. It is important that both the measurement and the calibration (zeroing) cuvette are optically identical to provide the same measurement conditions. Most methods use the same cuvette for both, so it is important that measurements are taken at the same optical point.
1) 2) 3) 4) 5) 6) 7) 8) Open Cuvette Lid Indexing mark Cuvette point Liquid Crystal Display (LCD) Splash proof keypad ON/OFF power switch Power input connector USB connector KEYPAD DESCRIPTION The keypad contains 8 direct keys and 3 functional keys with the following functions: Press to perform the function displayed above it on the LCD. Press to exit the current screen. Press to access the select method menu.
(b) pull the plunger up until the lower edge of the seal is exactly on the 0.0 mL mark. (c) take out the syringe and clean the outside of the syringe tip. Be sure that no drops are hanging on the tip of the syringe, if so eliminate them. Then, keeping the syringe in vertical position above the cuvette, push the plunger down into the syringe until the lower edge of the seal is exactly on the 0.5 mL mark. Now the exact amount of 0.
USING CUVETTES • Proper mixing is very important for reproducibility of the measurements. The right way of mixing a cuvette is specified for each method in the related chapter. (a) invert the cuvette a couple of times or for a specified time: hold the cuvette in the vertical position. Turn the cuvette upside-down and wait for all of the solution to flow to the cap end, then return the cuvette to the upright vertical position and wait for all of the solution to flow to the cuvette bottom.
• In order to avoid reagent leaking and to obtain more accurate measurements, close the cuvette first with the supplied HDPE plastic stopper and then the black cap. • Whenever the cuvette is placed into the measurement cell, it must be dry outside, and free of fingerprints, oil or dirt. Wipe it thoroughly with HI 731318 or a lint-free cloth prior to insertion. • Shaking the cuvette can generate bubbles in the sample, causing higher readings.
METHOD REFERENCE TABLE Method 1 2 3 4 5 6 Method description Ammonia HR Ammonia MR Ammonia LR Nitrate HR Nitrate MR Nitrate LR Page Method 23 25 27 29 31 33 7 8 9 10 11 Method description Phosphorus HR Phosphorus MR Phosphorus LR Potassium HR Potassium MR 12 Potassium LR Page 35 37 39 41 44 46 OPERATIONAL GUIDE POWER CONNECTION AND BATTERY MANAGEMENT The meter can be powered from an AC/DC adapter (included) or from the built-in rechargeable battery.
METHOD SELECTION • Turn the instrument ON via the ON/OFF power switch. • The meter will perform an autodiagnostic test. During this test, the Hanna Instrument logo will appear on the LCD. After 5 seconds, if the test was successful, the last method used will appear on the display. • In order to select the desired method press the METHOD key and a screen with the available methods will appear. • Press the keys to highlight the desired method. Press Select.
CHEMICAL FORM Chemical form conversion factors are pre-programmed into the instrument and are method specific. In order to view the displayed result in the desired chemical form press or to access the second level functions and then press the Chem Frm key to toggle between the available chemical forms for the selected method. SETUP In the Setup mode the instrument’s parameters can be changed.
Date / Time This option is used to set the instrument’s date and time. Press the Modify key to change the date/time. Press the W X functional keys to highlight the value to be modified (year, month, day, hour, minute or second). Use the keys to change the value. Press the Accept key to confirm or ESC to return to the setup without saving the new date or time. Time format Option: AM/PM or 24 hour. Press the functional key to select the desired time format.
Instrument ID Option: 0 to 9999. This option is used to set the instrument’s ID (identification number). The instrument ID is used while exchanging data with a PC. Press the Modify key to access the instrument ID screen. Press the keys in order to set the desired value. Press the Accept key to confirm the value or ESC to return to the setup menu without saving the new value. Meter information Press the Select key to view the instrument model, firmware version, language version and instrument serial number.
INTRODUCTION THE ROLE OF NUTRIENTS IN THE GROWTH AND PRODUCTION OF PLANTS The three elements that are mostly needed by the plants are nitrogen (N), phosphorus (P) and potassium (K). They are called the macronutrients while other elements, needed by plants in smaller amounts, are called microelements. In hydroponics, plants need a balanced nutritive solution, composed of macro and microelements.
PHOSPHORUS Phosphorus (P) has an important role in many fundamental biochemical and physiological processes. Plants take up phosphorus in the form of phosphate ion (PO43¯).
IRRIGATION WATER (LOW RANGE) In agricultural areas it is quite common to find altered values in the chemical composition of irrigation waters. The problem concerns mostly the high nitrate concentration, usually determined by excessive fertilization or irrational liquid manure spreading. The analysis of irrigation waters allows us to find out which are the substances present in major or minor quantity and to organize an advantageous fertilization plan.
SAMPLE PREPARATION SAMPLE PREPARATION PROCEDURE Note: If the water sample to be analyzed is very turbid, let it stand in a beaker for a while until most of the solid particles have settled. Then, use the pipette to transfer the supernatant solution to the other beaker and prepare the samples as described below. To prevent the displacement of the settled solids at the bottom of the beaker, do not induce air bubbles into the solution.
• Fold a filter disc twice as shown in the figure. Separate one side from the other three to form a cone. Insert the folded filter disc in the funnel. • Filter the treated sample into an empty beaker. The sample is now ready. Note: Filter at least 40 mL of solution if all the four methods will be tested. If the solution is still turbid or colored, treat it again with a packet of active carbon. After use, throw the filter disc away and wash the syringe and the filter assembly well.
• Replace the cap and shake gently for at least 2 minutes. • Open the upper part of the Demineralizer Bottle cap and gently squirt the demineralized water into the cylinder, up to the 100 mL mark. Note: The ion exchange resin contained in the Demineralizer Bottle is provided with an indicator substance. The indicator will change from green to blue when the resin has been exhausted and needs to be replaced. • Pour the solution in the large 170 mL beaker, replace the cap and invert several times to mix.
Note: To measure exactly 5 mL of sample with the syringe, push the plunger completely into the syringe and insert the tip into the sample. Pull the plunger out until the lower edge of the seal is on the 5 mL mark of the syringe. • Remove the cap and fill the Demineralizer Bottle with tap water. • Replace the cap and shake gently for at least 2 minutes. • Open the upper part of the Demineralizer Bottle cap and squirt gently the demineralized water into the cylinder, up to the 100 mL mark.
AMMONIA HIGH RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0 to 100 mg/L 1 mg/L ±1 mg/L ±4% of reading at 25 °C ±1 mg/L Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92, Nessler method. The reaction between ammonia and reagents causes a yellow tint in the sample.
• Remove the cuvette. • Add 4 drops of HI 93715A-0 First Reagent (6 drops for seawater analysis). Replace the cap and mix the solution by inverting the cuvette a couple of times. • Add 4 drops of HI 93715B-0 Second Reagent (10 drops for seawater analysis). Replace the cap and mix the solution by inverting the cuvette a couple of times. • Reinsert the cuvette into the instrument.
AMMONIA MEDIUM RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0.0 to 50.0 mg/L 0.5 mg/L ±0.5 mg/L ±4% of reading at 25 °C ±0.5 mg/L Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92, Nessler method. The reaction between ammonia and reagents causes a yellow tint in the sample.
• Remove the cuvette. • Add 4 drops of HI 93715A-0 First Reagent (6 drops for seawater analysis). Replace the cap and mix the solution by inverting the cuvette a couple of times. • Add 4 drops of HI 93715B-0 Second Reagent (10 drops for seawater analysis). Replace the cap and mix the solution by inverting the cuvette a couple of times. • Reinsert the cuvette into the instrument.
AMMONIA LOW RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0.0 to 10.0 mg/L 0.1 mg/L ±0.1 mg/L ±4% of reading at 25 °C ±0.1 mg/L Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92, Nessler method. The reaction between ammonia and reagents causes a yellow tint in the sample.
• Remove the cuvette. • Add 4 drops of HI 93715A-0 First Reagent (6 drops for seawater analysis). Replace the cap and mix the solution by inverting the cuvette a couple of times. • Add 4 drops of HI 93715B-0 Second Reagent (10 drops for seawater analysis). Replace the cap and mix the solution by inverting the cuvette a couple of times. • Reinsert the cuvette into the instrument.
NITRATE HIGH RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0 to 300 mg/L 5 mg/L ±10 mg/L ±8% of reading at 25 °C ±5 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the cadmium reduction method. The reaction between nitrate-nitrogen and the reagent causes an amber tint in the sample.
• Replace the cap and immediately shake vigorously up and down for exactly 10 seconds. Continue to mix by inverting the cuvette gently for 50 seconds, while taking care not to induce air bubbles. Powder will not completely dissolve. Time and way of shaking could sensitively affect the measurement. • Reinsert the cuvette into the instrument, taking care not to shake it.
NITRATE MEDIUM RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0 to 150 mg/L 2.5 mg/L ±5 mg/L ±8% of reading at 25 °C ±2.5 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the cadmium reduction method. The reaction between nitrate-nitrogen and the reagent causes an amber tint in the sample.
• Replace the cap and immediately shake vigorously up and down for exactly 10 seconds. Continue to mix by inverting the cuvette gently for 50 seconds, while taking care not to induce air bubbles. Powder will not completely dissolve. Time and way of shaking could sensitively affect the measurement. • Reinsert the cuvette into the instrument, taking care not to shake it.
NITRATE LOW RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0.0 to 30.0 mg/L 0.5 mg/L ±1.0 mg/L ±8% of reading at 25 °C ±0.5 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the cadmium reduction method. The reaction between nitrate-nitrogen and the reagent causes an amber tint in the sample.
• Replace the cap and immediately shake vigorously up and down for exactly 10 seconds. Continue to mix by inverting the cuvette gently for 50 seconds, while taking care not to induce air bubbles. Powder will not completely dissolve. Time and way of shaking could sensitively affect the measurement. • Reinsert the cuvette into the instrument, taking care not to shake it.
PHOSPHORUS HIGH RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Dev. Light Source Method 0 to 100 mg/L 1 mg/L ±5 mg/L ±4% of reading at 25 °C ±1 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Amino Acid method. The reaction between phosphorus and reagents causes a blue tint in the sample.
• Add the content of one packet of HI 93706B-0 Phosphorus Reagent B (Amino Acid) to the cuvette. Replace the cap and shake gently until completely dissolved. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 5 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of phosphorus (P). • Press or to access the second level functions.
PHOSPHORUS MEDIUM RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Dev. Light Source Method 0.0 to 50.0 mg/L 0.5 mg/L ±2.5 mg/L ±4% of reading at 25 °C ±0.5 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Amino Acid method. The reaction between phosphorus and reagents causes a blue tint in the sample.
• Add the content of one packet of HI 93706B-0 Phosphorus Reagent B (Amino Acid) to the cuvette. Replace the cap and shake gently until completely dissolved. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 5 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of phosphorus (P). • Press or to access the second level functions.
PHOSPHORUS LOW RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Dev. Light Source Method 0.0 to 10.0 mg/L 0.1 mg/L ±0.5 mg/L ±4% of reading at 25 °C ±0.1 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Amino Acid method. The reaction between phosphorus and reagents causes a blue tint in the sample.
• Add the content of one packet of HI 93706B-0 Phosphorus Reagent B (Amino Acid) to the cuvette. Replace the cap and shake gently until completely dissolved. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 5 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of phosphorus (P). • Press or to access the second level functions.
POTASSIUM HIGH RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 20 to 200 mg/L 5 mg/L ±30 mg/L ±7% of reading at 25 °C ±5 mg/L Tungsten lamp with narrow band interference filter @ 610 nm Adaptation of the Turbidimetric Tetraphenylborate method. The reaction between Potassium and reagents causes turbidity in the sample.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette and add the content of one packet of HI 93750B-0 reagent. Replace the cap and gently mix for one minute by slowly turning the cuvette upside down. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 2 minutes and press Read. When the timer ends the meter will perform the reading.
INTERFERENCES Interferences may be caused by: Ammonium above 10 ppm Calcium above 10000 ppm as CaCO3 Chloride above 12000 ppm Magnesium above 8000 ppm as CaCO3 Sodium above 8000 ppm POTASSIUM ULTRA HIGH RANGE For samples containing more than 200 ppm of Potassium: follow the sample preparation procedure described at page 21 for NUTRIENTS SOLUTION (HR).
POTASSIUM MEDIUM RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 10 to 100 mg/L 2.5 mg/L ±15 mg/L ±7% of reading at 25 °C ±2.5 mg/L Tungsten lamp with narrow band interference filter @ 610 nm Adaptation of the Turbidimetric Tetraphenylborate method. The reaction between Potassium and reagents causes turbidity in the sample.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette and add the content of one packet of HI 93750B-0 reagent. Replace the cap and gently mix for one minute by slowly turning the cuvette upside down. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 2 minutes and press Read. When the timer ends the meter will perform the reading.
POTASSIUM LOW RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0.0 to 20.0 mg/L 0.5 mg/L ±3.0 mg/L ±7% of reading at 25 °C ±0.5 mg/L Tungsten lamp with narrow band interference filter @ 610 nm Adaptation of the Turbidimetric Tetraphenylborate method. The reaction between Potassium and reagents causes turbidity in the sample.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette and add the content of one packet of HI 93750B-0 reagent. Replace the cap and gently mix for one minute by slowly turning the cuvette upside down. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 2 minutes and press Read. When the timer ends the meter will perform the reading.
ERRORS AND WARNINGS The instrument shows clear warning messages when erroneous conditions appear and when measured values are outside the expected range. These messages are described bellow. No Light: The light source is not functioning properly. Light Leak: There is an excess amount of ambient light reaching the detector. Inverted cuvettes: The sample and the zero cuvettes are inverted. Battery Low: The battery capacity is lower than 10%. Light Low: The instrument cannot adjust the light level.
DATA MANAGEMENT The analyzed data can be managed using Hanna’s product HI92000, Windows® Compatible Software. STANDARD METHODS Description Range Ammonia HR Ammonia MR Ammonia LR Nitrate HR Nitrate MR Nitrate LR Phosphorus HR Phosphorus MR Phosphorus LR Potassium HR Potassium MR Potassium LR 0 to 100 mg/L 0.0 to 50.0 mg/L 0.0 to 10.0 mg/L 0 to 300 mg/L 0 to 150 mg/L 0.0 to 30.0 mg/L 0 to 100 mg/L 0.0 to 50.0 mg/L 0.0 to 10.0 mg/L 20 to 200 mg/L 10 to 100 mg/L 0.0 to 20.
ACCESSORIES REAGENT SETS HI 93706-01 100 phosphorus tests HI 93706-03 300 phosphorus tests HI 93715-01 100 ammonia tests HI 93715-03 300 ammonia tests HI 93728-01 100 nitrate tests HI 93728-03 300 nitrate tests HI 93750-01 100 potassium tests HI 93750-03 300 potassium tests OTHER ACCESSORIES HI 731318 cloth for wiping cuvettes (4 pcs) HI 731321 glass cuvettes (4 pcs) HI 731325W new cap for cuvette (4 pcs) HI 740034 cap for 100 mL beaker (6 pcs) HI 740036 100 mL plastic beaker (6 pcs) HI 740157 plastic refil
WARRANTY All Hanna Instruments meters are warranted for two years against defects in workmanship and materials when used for its intended purpose and maintained according to the instructions. This warranty is limited to repair or replacement free of charge. Damages due to accident, misuse, tampering or lack of prescribed maintenance are not covered. If service is required, contact your dealer. If under warranty, report the model number, date of purchase, serial number and the nature of the failure.
Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA Technical Support for Customers Tel. (800) 426 6287 Fax (401) 765 7575 E-mail tech@hannainst.com www.hannainst.
