Instruction Manual HI 253 Microprocessor-Based pH/mV/Ion/ºC Bench Meter pH CAL Func tions CF GLPM GLP Show GLP Data Store Log Da ta Reca ll Log RANG Data E Selec Scrol t pH/O SET/C l Log RP/ LR Set ClearInstrume Data Rel mV/Io n Calib nt Pa ration ram /Log eters Data LOG C RCL C LOG RCL CA / CFM HI pH / 253 ORP / Ion Mete pH Ca libratio n-3 L points Start/E xit Ca librat Selec ion t pH Bu Co ffer in senfirm Ca RANG lected librat E pH Bu ion ffer SE CLR T r w w w. h a n n a i n s t .
Dear Customer, Thank you for choosing a Hanna Instruments product. Please read this instruction manual carefully before using the instrument. This manual will provide you with the necessary information for correct use of the instrument, as well as a precise idea of its versatility. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com or turn to the back cover for our worldwide contact list. This instrument is in compliance with directives.
PRELIMINARY EXAMINATION Remove the instrument from the packing material and examine it carefully to make sure that no damage has occurred during shipping. If there is any damage, notify your Dealer or the nearest Hanna Customer Service Center. Each instrument is supplied with: • HI 1131B Glass-body Combination pH Electrode with 1 m (3.3') Cable • HI 7669/2W Temperature Probe • HI 76404 Electrode Holder • pH 4.01 & 7.
FUNCTIONAL DESCRIPTION Primary LCD Secondary LCD Front Panel CAL LOG 1 % TIME DATE INTV BUFFER pH C mV pH CFM 1 ppm READY 2 WRONG BUF 3 10 2 Functions 3 4 CAL CFM GLP 5 Show GLP Data Store Log Data / RCL Recall Log Data CFM RANGE 6 C C LOG RCL pH Calibration - 3 points GLP LOG SET/CLR CAL Select pH Buffer RANGE Confirm Calibration in selected pH Buffer Select pH/ORP/Rel mV/Ion Scroll Log Data Set Instrument Parameters Clear Calibration/Log Data 7 9 Start/Exit Calibration 8
HI 253 SPECIFICATIONS –2.0 to 16.0 pH –2.00 to 16.00 pH –2.000 to 16.000 pH ±699.9 mV ±2000 mV RANGE 0.001 to 19999 ppm –9.9 to 120.0 ºC 0.1 pH 0.01 pH 0.001 pH 0.1 mV (±699.9 mV) 1 mV (±2000 mV) RE SOLUTION 0.001 (0.001 to 9.999) ppm 0.01 (10 to 99.99) ppm 0.1 (100 to 999.9) ppm 1 (1000 to 19999) ppm 0.1 ºC ±0.1 pH ±0.01 pH ±0.002 pH ±0.2 mV (±699.9 mV) ±1 mV (±2000 mV) ACCURACY @ 20 °C/68 °F ±0.5% f.s. ±0.
HI 253 SPECIFICATIONS (cont. ) Ion Calibration Up to two-point calibration, 5 buffers available (0.1, 1, 10, 100, 1000) Temperature compensation Manual or Automatic from: –9.9 to 120.0 ºC pH E lectrode HI 1131B Temperature probe HI 7669/2W Input impedance 1012 ohm Power supply 12 VD C adapter D imensions 240x182x74 mm (9.4x7.1x2.9”) Weight 1.1 Kg (2.5 lb); kit with holder 2.5 Kg (5.
OPERATIONAL GUIDE POWER CONNECTION Plug the 12 VDC adapter into the power supply socket. Note: This instrument uses non volatile memory to retain the pH, mV, temperature calibrations and all other settings, even when unplugged. Note: Make sure a fuse protects the main line. ELECTRODE AND PROBE CONNECTIONS For pH or ORP measurements connect an electrode with internal reference to the BNC connector on the back of the instrument.
If measurements are taken successively in different samples, it is recommended to rinse the electrode thoroughly with deionized water or tap water and then with some of the next sample to prevent cross-contamination. The pH reading is affected by temperature. In order to measure the pH accurately, the temperature effect must be compensated for.
RELATIVE mV MEASUREMENTS • Press RANGE until “rEL” message will be displayed on the secondary LCD line for about one second and “mV” tag will blink. After one second the temperature will be displayed on the secondary LCD. mV C The reading displayed by the instrument is equal to the difference between the current mV input value and relative mV offset established in the relative mV calibration. ION MEASUREMENTS • Press RANGE to enter ppm measurement mode.
pH CALIBRATION Calibrate the instrument frequently, especially if high accuracy is required. The instrument should be recalibrated : • Whenever the pH electrode is replaced. • At least once a week. • After testing aggressive chemicals. • If “CAL” “INTV” tags are blinking during measurement. Every time you calibrate the instrument use fresh buffers and perform an electrode Cleaning Procedure (see page 34). PREPARATION Pour small quantities of the buffer solutions into clean beakers.
• The “ ” tag will blink on the LCD until the reading is stable. • When the reading is stable and close to the selected buffer, the “READY” tag will be displayed and the “CFM” tag will blink. • Press CFM to confirm calibration. • The calibrated value will be displayed on the primary LCD and the second expected buffer value on the secondary LCD. CAL pH BUFFER pH BUF 2 Note: The instrument will automatically skip the buffer used for the first point. It also skips 6.86 if 7.01 buffer was used and viceversa.
Note: • If the value measured by the instrument is not close to the selected buffer, “WRONG” “ ” and “WRONG” “ ” tags will blink alternately. In this case, check if the correct buffer has been used or regenerate the electrode by following the Cleaning Procedure (see page 34). If necessary, change the buffer or the electrode. • If “WRONG”, “Buffer pH” tags and “Old” tag on the secondary LCD are displayed blinking, an inconsistency between new and previous (old) calibration is detected.
ION CALIBRATION For greatest accuracy, it is recommended to calibrate the instrument frequently. Due to electrode conditioning time, the electrode must be kept immersed a few seconds to stabilize. The user will be guided step by step during calibration with easy to follow tags on the display. This will make the calibration a simple and error-free procedure. PREPARATION In SETUP menu, select the proper ion charge.
Note: The instrument will automatically skip the buffer used for the first point. • After the first calibration point is confirmed, immerse the ISE electrode approximately 4 cm (1½”) into the second calibration solution. • If necessary, press the ARROW keys to select a different buffer value. • The “ ” tag will blink on the LCD until the reading is stable. • When the reading is stable and close to the selected buffer, the “READY” tag will be displayed and the “CFM” tag will blink.
GOOD LABORATORY PRACTICE (GLP) GLP is a set of functions that allows storage and retrieval of data regarding the maintenance and status of the electrode. All data regarding last pH calibration (one, two or three-point) and Ion calibration is stored for the user to review when necessary. This data includes the following: calibration time stamp, offset (mV), average of pH slope (%) or Ion slope (mV), calibration buffers and the time until a new calibration is required (only for pH calibration).
• The pH calibration slope (the GLP slope is the average of the calibration slopes; the percentage is referred to the ideal value of 59.16 mV/pH). % CAL • The pH calibration buffers in calibrating order. The first pH calibration buffer: CAL pH BUF The second pH calibration buffer: CAL pH BUF The third pH calibration buffer: CAL pH BUF Note: • The “OLd” message displayed beside the pH value means that this buffer was not used during last calibration.
or the number of days until the calibration alarm will be displayed. or if expired (7 days ago). INTV CAL • The instrument ID. LAST ION CALIBRATION DATA Last Ion calibration data is stored automatically after a successful calibration. To view Ion calibration data, press GLP when the instrument is in Ion measurement mode. The instrument will display the time (hh:mm) of the last calibration.
• The second mV value. CAL mV BUF 2 • The Ion calibration slope (mV/decade). CAL mV • The instrument ID. Note: • If a one-point calibration is performed after a two-point calibration, the instrument will keep the old slope. • If “no bUF” message appears on the LCD, the instrument informs you that calibration was performed in only one point. • Press GLP at any moment and the instrument will return to measurement mode.
If the log space is full, “FULL LOC” message will be displayed and no more data will be saved. LOG When LOG is pressed, a complete set of information is stored: date, time, pH or ppm, mV, temperature and calibration data. VIEW LOGGED DATA Press RCL to retrive the information stored while in measurement mode.
• The temperature value on the primary LCD and the record number on the secondary LCD. LOG C Note: When the pH, mV or temperature is displayed, if SET/CLR is pressed and hold down, the secondary LCD will display the record date. • The time on the primary LCD, along with “TIME” tag. TIME LOG • The date on the primary LCD, along with “DATE” tag. DATE LOG • The calibration offset on the primary LCD and “OFS” message on the secondary LCD.
• The “dEL” message on the primary LCD and the selected record on the secondary LCD, along with ”LOG” tag. LOG Note: • If one of the ARROW keys is pressed while “dEL” and record number is displayed, the next/previous record number is selected. • If LOG is pressed, the secondary LCD will display “ALL” message. LOG • Pressing LOG again, the secondary LCD will display the record number. • Press SET/CLR to delete the selected or all records.
SETUP Setup mode allows viewing and modifying the following parameters: • Current Time • Current Date • pH Resolution • Ion Charge • Beep Status • Baud Rate (serial communication) • Command prefix (serial communication) • Calibration Alarm Time-Out • Instrument ID To enter SETUP mode press SET while the instrument is in measurement mode. Select a parameter with the ARROW keys. Press CAL if you want to change a parameter value. The selected parameter will start blinking.
CURRENT DATE SET Press CAL when the current date is displayed. The year will start blinking. DATE Press the ARROW keys to change the year. Press RANGE. The month will start blinking. DATE Press the ARROW keys to change the month. Press RANGE. The day will start blinking. DATE Press the ARROW keys to change the day. Press CFM to save the modified value. Press CAL to escape without saving. pH RESOLUTION SET Press CAL when the pH range is displayed. The pH range (0.1, 0.01 or 0.001) will start blinking.
Press the ARROW keys to change the ion charge. Note: To select the right ion charge, different ion types and their charge are presented in the table below. ION CHARGE ION t ypes –2 (divalent anions) S, CO3 –1 (monovalent anions) F, Cl, Br, I, CN, SCN, ClO4, NO3 1 (monovalent cations) Na, K, Ag, NH4 2 (divalent cations) Mg, Ca, Ba, Cd, Cu, Pb undF undefined ion Press CFM to save the modified ion charge. Press CAL to escape without saving.
SERIAL COMMUNICATION COMMAND PREFIX SET Press CAL when the command prefix is displayed. The command prefix (0 to 47) will start blinking. Press the ARROW keys to change the command prefix. Press CFM to save the modified command prefix value. Press CAL to escape without saving. Note: • See the PC interface section on page 29 for a complete explanation. • The command prefix must be 16 if HI 92000 PC software is used. CALIBRATION ALARM TIME-OUT SET Press CAL when the calibration time-out is displayed.
TEMPERATURE CALIBRATION (for technical personnel only) All the instruments are factory calibrated for temperature. Hanna’s temperature probes are interchangeable and no temperature calibration is needed when they are replaced. If the temperature measurements are inaccurate, temperature recalibration should be performed. For an accurate recalibration, contact your dealer or the nearest Hanna Customer Service Center, or follow the instructions bellow.
• Use the ARROW keys to set the reading on the secondary LCD to that of the hot water. C C • When the reading is stable and close to the selected calibration point, “READY” tag will appear and “CFM” tag will blink. • Press CFM to confirm. The instrument returns to measurement mode. Note: If the reading is not close to the selected calibration point, “WRONG” tag will blink. Change the temperature probe and restart calibration.
Note: • If the reading is not close to the selected calibration point, “WRONG” tag will blink. Verify calibration condition or contact your vendor if you can not calibrate. • Press CAL in any moment of the calibration process. The instrument will return to measurement mode. If calibration process is stopped after 600 mV is confirmed, the 600 mV range is calibrated and calibration parameters are memorized. RELATIVE mV CALIBRATION • Press CAL when the instrument is in RELATIVE mV measurement mode.
PC INTERFACE Data transmission from the instrument to the PC can be done with the HI 92000 Windows® compatible software (optional). HI 92000 also offers graphing and on-line help feature. Data can be exported to the most popular spreadsheet programs for further analysis. To connect your instrument to a PC, use the optional Hanna HI 920010 cable connector. Make sure that your instrument is switched off and plug one connector to the instrument RS232C socket and the other into the serial port of your PC.
CHR n Change the instrument range according with the parameter value (n): • n=0 pH range/0.001 resolution • n=1 pH range/0.01 resolution • n=2 pH range/0.1 resolution • n=3 mV range • n=4 Relative mV range • n=5 Ion range COMMANDS REQUIRING AN ANSWER pH? MV? MR? TM? pM? RAS MDR PAR NSL GLP LOD xxx LOD ALL Note: • • • Causes the instrument to send the pH reading. If the range is set to mV, “Err6” is received. Causes the instrument to send the mV reading. If the range is set to pH, “Err6” is received.
pH VALUES AT DIFFERENT TEMPERATURES The temperature has an effect on pH. The calibration buffer solutions are affected by temperature changes to a lesser degree than normal solutions. During calibration the instrument will automatically calibrate to the pH value corresponding to the measured or set temperature. TEMP pH VALUES ºC ºF 4.01 6.86 7.01 9.18 10.01 0 32 4.01 6.98 7.13 9.46 10.32 5 41 4.00 6.95 7.10 9.39 10.24 10 50 4.00 6.92 7.07 9.33 10.18 15 59 4.00 6.90 7.
ELECTRODE CONDITIONING & MAINTENANCE PREPARATION PROCEDURE Remove the protective cap of the pH electrode. DO NOT BE ALARMED IF SALT DEPOSITS ARE PRESENT. This is normal with electrodes. They will disappear when rinsed with water. During transport, tiny bubbles of air may form inside the glass bulb affecting proper functioning of the electrode. These bubbles can be removed by “shaking down” the electrode as you would do with a glass thermometer.
For refillable electrodes: If the filling solution (electrolyte) is more than 2½ cm (1”) below the fill hole, add HI 7082 or HI 8082 3.5M KCl Electrolyte Solution for double junction or HI 7071 or HI 8071 3.5M KCl+AgCl Electrolyte Solution for single junction electrodes. For faster response, unscrew the fill hole screw during measurements. For AMPHEL ® electrodes: If the electrode does not respond to pH changes, the battery is run down and the electrode should be replaced.
CLEANING PROCEDURE • General Soak in Hanna HI 7061 or HI 8061 General Cleaning Solution for approximately ½ hour. • Protein Soak in Hanna HI 7073 or HI 8073 Protein Cleaning Solution for 15 minutes. • Inorganic Soak in Hanna HI 7074 Inorganic Cleaning Solution for 15 minutes. • Oil/grease Rinse with Hanna HI 7077 or HI 8077 Oil and Fat Cleaning Solution.
TROUBLESHOOTING GUIDE SYMPTOMS Slow reponse/excessive drift. PROBLEM D irty pH electrode. SOLUTION Soak the electrode tip in HI 7061 or HI 8061 solution for 30 minutes and then clean the electrode. Readings fluctuate up Clogged/dirty junction. and down (noise). Low electrolyte level (refillable electrodes only). Clean the electrode. Refill with fresh solution (for refillable electrodes only). Out of range in the mV scale. D ry membrane/junction. Soak in HI 70300 or HI 80300 storage solution.
TEMPERATURE CORRELATION FOR pH SENSITIVE GLASS The resistance of glass electrodes partially depends on the temperature. The lower the temperature, the higher the resistance. It takes more time for the reading to stabilize if the resistance is higher. In addition, the response time will suffer to a greater degree at temperatures below 25 ºC. Since the resistance of the pH electrode is in the range of 50 – 200 Mohm, the current across the membrane is in the pico Ampere range.
ACCESSORIES pH BUFFER SOLUTIONS HI HI HI HI HI HI HI HI HI HI HI HI HI HI HI 70004P 70007P 70010P 7001L 7004L 7006L 7007L 7009L 7010L 7001L 8004L 8006L 8007L 8009L 8010L pH 4.01 Buffer Sachets, 20 mL, 25 pcs pH 7.01 Buffer Sachets, 20 mL, 25 pcs pH 10.01 Buffer Sachets, 20 mL, 25 pcs pH 1.68 Buffer Solution, 500 mL pH 4.01 Buffer Solution, 500 mL pH 6.86 Buffer Solution, 500 mL pH 7.01 Buffer Solution, 500 mL pH 9.18 Buffer Solution, 500 mL pH 10.01 Buffer Solution, 500 mL pH 1.
ORP PRETREATMENT SOLUTIONS HI 7091L Reducing Pretreatment Solution, 460 mL HI 7092L Oxidizing Pretreatment Solution, 460 mL pH ELECTRODES All electrodes part numbers ending in B are supplied with a BNC connector and 1 m (3.3') cable, as shown below : HI 1043B Glass-body, double junction, refillable, combination pH electrode. Use: strong acid/alkali. 9.5mm DIA 0.37" 12 mm 0.5" HI 1043 120 mm 4.7" "S" VERSION HI 1053B Glass-body, triple ceramic, conic shape, refillable, combination pH electrode.
HI 1330B Glass-body, semimicro, single junction, refillable, combination pH electrode. Use: laboratory, vials. 5mm DIA 0.2" 5mm 0.2" HI 1330 120 mm 4.7" "S" VERSION HI 1331B Glass-body, semimicro, single junction, refillable, combination pH electrode. Use: flasks. HI 1310 8 mm 0.3" 7.5mm DIA 0.29" HI 1331 210 mm 8.25" "S" VERSION HI 1230B Plastic-body (Ultem®), double junction, gel-filled, combination pH electrode. Use: general, field. HI 1311 12 mm 0.5" HI 1230 120 mm 4.
FC 100B Plastic-body (Kynar®), double junction, refillable, combination pH electrode. Use: general purpose for food industry. 12 mm 0.5" FC 100 120 mm 4.7" FC 200B Plastic-body (Kynar®), open junction, conic, Viscolene, non-refillable, combination pH electrode. Use: meat & cheese. 6 mm 0.25" FC 200 75 mm 2.95" FC 210B Glass-body, double junction, conic, Viscolene, non-refillable, combination pH electrode. Use: milk, yogurt. 12 mm 0.5" FC 210 120 mm 4.
HI 1413B Glass-body, single junction, flat tip, Viscolene, non-refillable, combination pH electrode. Use: surface measurement. 12 mm 0.5" HI 1413 110 mm 4.3" ORP ELECTRODES HI 3131B Glass-body, refillable, combination platinum ORP electrode. Use: titration. 12 mm 0.5" HI 3131 150 mm 5.9" "S" VERSION HI 3230B Plastic-body (Ultem®), gel-filled, combination platinum ORP electrode. Use: general purpose. HI 3111 12 mm 0.5" HI 3230 120 mm 4.
EXTENSION CABLE FOR SCREW-TYPE ELECTRODES (SCREW TO BNC ADAPTER) HI 7855/1 Extension cable 1 m (3.3') long HI 7855/3 Extension cable 3 m (9.9') long HI 7855 SERIES CABLE CONNECTORS CONNECTOR AND 3.0 mm (0.
RECOMMENDATIONS FOR USERS Before using this product, make sure that it is entirely suitable for the environment in which it is used. Operation of this instrument in residential areas could cause unacceptable interferences to radio and TV equipment, requiring the operator to follow all necessary steps to correct interferences. The glass bulb at the end of the pH electrode is sensitive to electrostatic discharges. Avoid touching this glass bulb at all times.
SALES AND TECHNICAL SERVICE CONTACTS Australia: Tel. (03) 9769.0666 • Fax (03) 9769.0699 China: Tel. (10) 88570068 • Fax (10) 88570060 Egypt: Tel. & Fax (02) 2758.683 Germany: Tel. (07851) 9129-0 • Fax (07851) 9129-99 Greece: Tel. (210) 823.5192 • Fax (210) 884.0210 Indonesia: Tel. (21) 4584.2941 • Fax (21) 4584.2942 Japan: Tel. (03) 3258.9565 • Fax (03) 3258.9567 Korea: Tel. (02) 2278.5147 • Fax (02) 2264.1729 Malaysia: Tel. (603) 5638.9940 • Fax (603) 5638.9829 Singapore: Tel. 6296.
